Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01365 and MMJJ_RS01370 are separated by 40 nucleotides MMJJ_RS01370 and MMJJ_RS01375 are separated by 13 nucleotides MMJJ_RS01375 and MMJJ_RS01380 are separated by 39 nucleotides
MMJJ_RS01365: MMJJ_RS01365 - Mrp/NBP35 family ATP-binding protein, at 264,032 to 264,901
_RS01365
MMJJ_RS01370: MMJJ_RS01370 - MnmC family methyltransferase, at 264,942 to 265,964
_RS01370
MMJJ_RS01375: MMJJ_RS01375 - tetrahydromethanopterin:alpha-L-glutamate ligase, at 265,978 to 266,856
_RS01375
MMJJ_RS01380: MMJJ_RS01380 - DUF1890 domain-containing protein, at 266,896 to 267,339
_RS01380
Position (kb) 264
265
266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 263.948 kb on - strand at 263.950 kb on - strand at 263.950 kb on - strand at 263.951 kb on + strand at 263.978 kb on - strand at 263.978 kb on - strand at 263.978 kb on - strand at 263.979 kb on + strand at 263.979 kb on + strand at 263.981 kb on - strand at 263.981 kb on - strand at 263.987 kb on + strand at 263.993 kb on - strand at 263.993 kb on - strand at 263.994 kb on + strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.995 kb on - strand at 263.996 kb on + strand at 263.996 kb on + strand at 263.996 kb on + strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.997 kb on - strand at 263.998 kb on + strand at 263.998 kb on + strand at 263.998 kb on + strand at 264.004 kb on - strand at 264.005 kb on + strand at 264.009 kb on + strand at 264.012 kb on - strand at 264.012 kb on - strand at 264.061 kb on - strand at 264.136 kb on - strand, within MMJJ_RS01365 at 264.152 kb on - strand, within MMJJ_RS01365 at 264.255 kb on - strand, within MMJJ_RS01365 at 264.336 kb on - strand, within MMJJ_RS01365 at 264.338 kb on - strand, within MMJJ_RS01365 at 264.338 kb on - strand, within MMJJ_RS01365 at 264.384 kb on + strand, within MMJJ_RS01365 at 264.412 kb on - strand, within MMJJ_RS01365 at 264.414 kb on - strand, within MMJJ_RS01365 at 264.489 kb on - strand, within MMJJ_RS01365 at 264.520 kb on - strand, within MMJJ_RS01365 at 264.591 kb on - strand, within MMJJ_RS01365 at 264.618 kb on - strand, within MMJJ_RS01365 at 264.655 kb on - strand, within MMJJ_RS01365 at 264.681 kb on - strand, within MMJJ_RS01365 at 264.709 kb on - strand, within MMJJ_RS01365 at 264.750 kb on - strand, within MMJJ_RS01365 at 264.827 kb on + strand at 264.838 kb on - strand at 264.899 kb on - strand at 264.899 kb on - strand at 264.904 kb on - strand at 264.938 kb on + strand at 265.036 kb on - strand at 265.140 kb on + strand, within MMJJ_RS01370 at 265.262 kb on + strand, within MMJJ_RS01370 at 265.262 kb on + strand, within MMJJ_RS01370 at 265.265 kb on - strand, within MMJJ_RS01370 at 265.265 kb on - strand, within MMJJ_RS01370 at 265.265 kb on - strand, within MMJJ_RS01370 at 265.265 kb on - strand, within MMJJ_RS01370 at 265.266 kb on + strand, within MMJJ_RS01370 at 265.266 kb on + strand, within MMJJ_RS01370 at 265.267 kb on - strand, within MMJJ_RS01370 at 265.267 kb on - strand, within MMJJ_RS01370 at 265.267 kb on - strand, within MMJJ_RS01370 at 265.267 kb on - strand, within MMJJ_RS01370 at 265.268 kb on + strand, within MMJJ_RS01370 at 265.271 kb on + strand, within MMJJ_RS01370 at 265.271 kb on + strand, within MMJJ_RS01370 at 265.271 kb on + strand, within MMJJ_RS01370 at 265.275 kb on - strand, within MMJJ_RS01370 at 265.297 kb on - strand, within MMJJ_RS01370 at 265.334 kb on - strand, within MMJJ_RS01370 at 265.351 kb on - strand, within MMJJ_RS01370 at 265.374 kb on + strand, within MMJJ_RS01370 at 265.453 kb on - strand, within MMJJ_RS01370 at 265.478 kb on + strand, within MMJJ_RS01370 at 265.493 kb on + strand, within MMJJ_RS01370 at 265.493 kb on + strand, within MMJJ_RS01370 at 265.493 kb on + strand, within MMJJ_RS01370 at 265.496 kb on - strand, within MMJJ_RS01370 at 265.497 kb on + strand, within MMJJ_RS01370 at 265.557 kb on - strand, within MMJJ_RS01370 at 265.646 kb on - strand, within MMJJ_RS01370 at 265.646 kb on - strand, within MMJJ_RS01370 at 265.647 kb on + strand, within MMJJ_RS01370 at 265.657 kb on + strand, within MMJJ_RS01370 at 265.729 kb on - strand, within MMJJ_RS01370 at 265.733 kb on - strand, within MMJJ_RS01370 at 265.734 kb on + strand, within MMJJ_RS01370 at 265.786 kb on - strand, within MMJJ_RS01370 at 265.839 kb on + strand, within MMJJ_RS01370 at 265.842 kb on - strand, within MMJJ_RS01370 at 265.843 kb on + strand, within MMJJ_RS01370 at 265.843 kb on + strand, within MMJJ_RS01370 at 265.843 kb on + strand, within MMJJ_RS01370 at 265.853 kb on - strand, within MMJJ_RS01370 at 265.862 kb on - strand at 265.863 kb on + strand at 265.876 kb on - strand at 265.876 kb on - strand at 265.913 kb on + strand at 265.951 kb on + strand at 265.979 kb on - strand at 266.032 kb on - strand at 266.033 kb on + strand at 266.078 kb on + strand, within MMJJ_RS01375 at 266.114 kb on + strand, within MMJJ_RS01375 at 266.114 kb on + strand, within MMJJ_RS01375 at 266.119 kb on - strand, within MMJJ_RS01375 at 266.119 kb on - strand, within MMJJ_RS01375 at 266.119 kb on - strand, within MMJJ_RS01375 at 266.120 kb on + strand, within MMJJ_RS01375 at 266.174 kb on + strand, within MMJJ_RS01375 at 266.247 kb on - strand, within MMJJ_RS01375 at 266.250 kb on + strand, within MMJJ_RS01375 at 266.336 kb on - strand, within MMJJ_RS01375 at 266.337 kb on + strand, within MMJJ_RS01375 at 266.337 kb on + strand, within MMJJ_RS01375 at 266.358 kb on - strand, within MMJJ_RS01375 at 266.398 kb on + strand, within MMJJ_RS01375 at 266.410 kb on - strand, within MMJJ_RS01375 at 266.411 kb on + strand, within MMJJ_RS01375 at 266.411 kb on + strand, within MMJJ_RS01375 at 266.447 kb on + strand, within MMJJ_RS01375 at 266.466 kb on - strand, within MMJJ_RS01375 at 266.562 kb on - strand, within MMJJ_RS01375 at 266.599 kb on - strand, within MMJJ_RS01375 at 266.599 kb on - strand, within MMJJ_RS01375 at 266.600 kb on + strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.605 kb on - strand, within MMJJ_RS01375 at 266.609 kb on - strand, within MMJJ_RS01375 at 266.621 kb on - strand, within MMJJ_RS01375 at 266.621 kb on - strand, within MMJJ_RS01375 at 266.621 kb on - strand, within MMJJ_RS01375 at 266.626 kb on - strand, within MMJJ_RS01375 at 266.627 kb on + strand, within MMJJ_RS01375 at 266.627 kb on + strand, within MMJJ_RS01375 at 266.674 kb on + strand, within MMJJ_RS01375 at 266.706 kb on - strand, within MMJJ_RS01375 at 266.725 kb on + strand, within MMJJ_RS01375 at 266.764 kb on - strand, within MMJJ_RS01375 at 266.764 kb on - strand, within MMJJ_RS01375 at 266.767 kb on + strand, within MMJJ_RS01375 at 266.779 kb on - strand at 266.779 kb on - strand at 266.779 kb on - strand at 266.780 kb on + strand at 266.857 kb on + strand at 266.857 kb on + strand at 266.859 kb on - strand at 266.868 kb on + strand at 266.888 kb on - strand at 266.889 kb on + strand at 266.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 263,948 - +0.4 263,950 - +1.1 263,950 - -0.5 263,951 + -1.7 263,978 - -1.1 263,978 - -0.5 263,978 - +0.9 263,979 + -0.2 263,979 + +0.4 263,981 - +0.9 263,981 - -0.9 263,987 + +2.3 263,993 - +1.2 263,993 - -0.2 263,994 + -0.5 263,995 - -0.7 263,995 - -1.3 263,995 - -0.4 263,995 - -1.2 263,996 + -2.8 263,996 + -1.9 263,996 + -0.2 263,997 - -1.4 263,997 - +0.3 263,997 - +0.5 263,997 - -1.4 263,998 + +1.0 263,998 + -2.4 263,998 + -1.5 264,004 - -3.0 264,005 + +3.2 264,009 + +1.9 264,012 - +0.7 264,012 - +1.9 264,061 - +0.6 264,136 - MMJJ_RS01365 0.12 -3.6 264,152 - MMJJ_RS01365 0.14 -1.1 264,255 - MMJJ_RS01365 0.26 -0.5 264,336 - MMJJ_RS01365 0.35 +1.3 264,338 - MMJJ_RS01365 0.35 +0.5 264,338 - MMJJ_RS01365 0.35 -0.5 264,384 + MMJJ_RS01365 0.40 -3.1 264,412 - MMJJ_RS01365 0.44 -3.1 264,414 - MMJJ_RS01365 0.44 +0.4 264,489 - MMJJ_RS01365 0.53 +0.3 264,520 - MMJJ_RS01365 0.56 -1.4 264,591 - MMJJ_RS01365 0.64 -1.3 264,618 - MMJJ_RS01365 0.67 -0.5 264,655 - MMJJ_RS01365 0.72 -1.3 264,681 - MMJJ_RS01365 0.75 -3.9 264,709 - MMJJ_RS01365 0.78 +0.8 264,750 - MMJJ_RS01365 0.83 -1.2 264,827 + +1.4 264,838 - -1.6 264,899 - -0.7 264,899 - -3.4 264,904 - +0.2 264,938 + -3.8 265,036 - +0.4 265,140 + MMJJ_RS01370 0.19 -0.2 265,262 + MMJJ_RS01370 0.31 -0.6 265,262 + MMJJ_RS01370 0.31 -0.9 265,265 - MMJJ_RS01370 0.32 +0.3 265,265 - MMJJ_RS01370 0.32 +0.3 265,265 - MMJJ_RS01370 0.32 -1.2 265,265 - MMJJ_RS01370 0.32 -0.5 265,266 + MMJJ_RS01370 0.32 -2.2 265,266 + MMJJ_RS01370 0.32 -0.8 265,267 - MMJJ_RS01370 0.32 -3.3 265,267 - MMJJ_RS01370 0.32 -0.1 265,267 - MMJJ_RS01370 0.32 -0.3 265,267 - MMJJ_RS01370 0.32 -1.0 265,268 + MMJJ_RS01370 0.32 -0.6 265,271 + MMJJ_RS01370 0.32 +0.1 265,271 + MMJJ_RS01370 0.32 -2.1 265,271 + MMJJ_RS01370 0.32 +0.9 265,275 - MMJJ_RS01370 0.33 -0.3 265,297 - MMJJ_RS01370 0.35 -0.6 265,334 - MMJJ_RS01370 0.38 -1.9 265,351 - MMJJ_RS01370 0.40 -0.6 265,374 + MMJJ_RS01370 0.42 -2.5 265,453 - MMJJ_RS01370 0.50 -0.4 265,478 + MMJJ_RS01370 0.52 -0.2 265,493 + MMJJ_RS01370 0.54 -1.6 265,493 + MMJJ_RS01370 0.54 -3.0 265,493 + MMJJ_RS01370 0.54 -1.5 265,496 - MMJJ_RS01370 0.54 -0.4 265,497 + MMJJ_RS01370 0.54 -0.3 265,557 - MMJJ_RS01370 0.60 +1.7 265,646 - MMJJ_RS01370 0.69 -1.4 265,646 - MMJJ_RS01370 0.69 -0.5 265,647 + MMJJ_RS01370 0.69 +0.4 265,657 + MMJJ_RS01370 0.70 -1.8 265,729 - MMJJ_RS01370 0.77 -0.9 265,733 - MMJJ_RS01370 0.77 +0.4 265,734 + MMJJ_RS01370 0.77 +0.3 265,786 - MMJJ_RS01370 0.83 +0.6 265,839 + MMJJ_RS01370 0.88 -0.9 265,842 - MMJJ_RS01370 0.88 -0.3 265,843 + MMJJ_RS01370 0.88 +1.6 265,843 + MMJJ_RS01370 0.88 -0.1 265,843 + MMJJ_RS01370 0.88 -2.6 265,853 - MMJJ_RS01370 0.89 -0.5 265,862 - -1.9 265,863 + +0.9 265,876 - -0.5 265,876 - +1.4 265,913 + -3.9 265,951 + -1.0 265,979 - -0.3 266,032 - -3.7 266,033 + -0.2 266,078 + MMJJ_RS01375 0.11 +1.7 266,114 + MMJJ_RS01375 0.15 -1.7 266,114 + MMJJ_RS01375 0.15 +1.0 266,119 - MMJJ_RS01375 0.16 +2.2 266,119 - MMJJ_RS01375 0.16 +0.7 266,119 - MMJJ_RS01375 0.16 +0.4 266,120 + MMJJ_RS01375 0.16 -0.9 266,174 + MMJJ_RS01375 0.22 -2.5 266,247 - MMJJ_RS01375 0.31 +1.6 266,250 + MMJJ_RS01375 0.31 +2.8 266,336 - MMJJ_RS01375 0.41 -2.0 266,337 + MMJJ_RS01375 0.41 +1.0 266,337 + MMJJ_RS01375 0.41 +0.8 266,358 - MMJJ_RS01375 0.43 -0.9 266,398 + MMJJ_RS01375 0.48 -1.1 266,410 - MMJJ_RS01375 0.49 +1.7 266,411 + MMJJ_RS01375 0.49 -2.0 266,411 + MMJJ_RS01375 0.49 +1.2 266,447 + MMJJ_RS01375 0.53 +1.2 266,466 - MMJJ_RS01375 0.56 +1.4 266,562 - MMJJ_RS01375 0.66 -1.2 266,599 - MMJJ_RS01375 0.71 +2.2 266,599 - MMJJ_RS01375 0.71 +0.6 266,600 + MMJJ_RS01375 0.71 +1.7 266,605 - MMJJ_RS01375 0.71 -1.6 266,605 - MMJJ_RS01375 0.71 +0.8 266,605 - MMJJ_RS01375 0.71 -0.2 266,605 - MMJJ_RS01375 0.71 +0.4 266,609 - MMJJ_RS01375 0.72 -0.2 266,621 - MMJJ_RS01375 0.73 +0.0 266,621 - MMJJ_RS01375 0.73 +1.5 266,621 - MMJJ_RS01375 0.73 -1.9 266,626 - MMJJ_RS01375 0.74 -1.4 266,627 + MMJJ_RS01375 0.74 +2.1 266,627 + MMJJ_RS01375 0.74 -0.2 266,674 + MMJJ_RS01375 0.79 +1.7 266,706 - MMJJ_RS01375 0.83 +0.7 266,725 + MMJJ_RS01375 0.85 +0.2 266,764 - MMJJ_RS01375 0.89 +1.3 266,764 - MMJJ_RS01375 0.89 +0.3 266,767 + MMJJ_RS01375 0.90 +0.3 266,779 - +1.9 266,779 - -1.7 266,779 - +0.7 266,780 + +0.6 266,857 + +0.6 266,857 + +0.8 266,859 - -3.1 266,868 + -1.3 266,888 - +1.2 266,889 + -2.5 266,889 + +0.5
Or see this region's nucleotide sequence