Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00425

Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00415 and MMJJ_RS00420 are separated by 14 nucleotidesMMJJ_RS00420 and MMJJ_RS00425 are separated by 185 nucleotidesMMJJ_RS00425 and MMJJ_RS00430 are separated by 28 nucleotidesMMJJ_RS00430 and MMJJ_RS00435 are separated by 14 nucleotides MMJJ_RS00415: MMJJ_RS00415 - DedA family protein, at 76,461 to 76,943 _RS00415 MMJJ_RS00420: MMJJ_RS00420 - CBS domain-containing protein, at 76,958 to 77,338 _RS00420 MMJJ_RS00425: MMJJ_RS00425 - hypothetical protein, at 77,524 to 77,916 _RS00425 MMJJ_RS00430: MMJJ_RS00430 - ribonuclease Z, at 77,945 to 78,871 _RS00430 MMJJ_RS00435: MMJJ_RS00435 - hypothetical protein, at 78,886 to 79,464 _RS00435 Position (kb) 77 78Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 76.582 kb on + strand, within MMJJ_RS00415at 76.647 kb on - strand, within MMJJ_RS00415at 76.647 kb on - strand, within MMJJ_RS00415at 76.680 kb on + strand, within MMJJ_RS00415at 76.751 kb on - strand, within MMJJ_RS00415at 76.751 kb on - strand, within MMJJ_RS00415at 76.763 kb on - strand, within MMJJ_RS00415at 76.783 kb on + strand, within MMJJ_RS00415at 76.833 kb on + strand, within MMJJ_RS00415at 76.833 kb on + strand, within MMJJ_RS00415at 76.836 kb on - strand, within MMJJ_RS00415at 76.837 kb on + strand, within MMJJ_RS00415at 76.837 kb on + strand, within MMJJ_RS00415at 76.838 kb on - strand, within MMJJ_RS00415at 76.839 kb on + strand, within MMJJ_RS00415at 76.839 kb on + strand, within MMJJ_RS00415at 76.839 kb on + strand, within MMJJ_RS00415at 76.845 kb on - strand, within MMJJ_RS00415at 76.903 kb on - strandat 76.908 kb on + strandat 76.908 kb on + strandat 76.911 kb on - strandat 76.982 kb on - strandat 76.998 kb on + strand, within MMJJ_RS00420at 76.999 kb on - strand, within MMJJ_RS00420at 77.030 kb on - strand, within MMJJ_RS00420at 77.045 kb on - strand, within MMJJ_RS00420at 77.108 kb on - strand, within MMJJ_RS00420at 77.138 kb on - strand, within MMJJ_RS00420at 77.138 kb on - strand, within MMJJ_RS00420at 77.138 kb on - strand, within MMJJ_RS00420at 77.139 kb on + strand, within MMJJ_RS00420at 77.139 kb on + strand, within MMJJ_RS00420at 77.199 kb on + strand, within MMJJ_RS00420at 77.199 kb on + strand, within MMJJ_RS00420at 77.199 kb on + strand, within MMJJ_RS00420at 77.200 kb on - strand, within MMJJ_RS00420at 77.222 kb on - strand, within MMJJ_RS00420at 77.222 kb on - strand, within MMJJ_RS00420at 77.223 kb on + strand, within MMJJ_RS00420at 77.228 kb on - strand, within MMJJ_RS00420at 77.229 kb on + strand, within MMJJ_RS00420at 77.252 kb on + strand, within MMJJ_RS00420at 77.262 kb on + strand, within MMJJ_RS00420at 77.266 kb on - strand, within MMJJ_RS00420at 77.367 kb on - strandat 77.370 kb on - strandat 77.370 kb on - strandat 77.371 kb on + strandat 77.372 kb on - strandat 77.372 kb on - strandat 77.373 kb on + strandat 77.373 kb on + strandat 77.374 kb on - strandat 77.374 kb on - strandat 77.375 kb on + strandat 77.379 kb on + strandat 77.427 kb on + strandat 77.441 kb on - strandat 77.441 kb on - strandat 77.445 kb on - strandat 77.446 kb on + strandat 77.446 kb on + strandat 77.446 kb on + strandat 77.446 kb on + strandat 77.446 kb on + strandat 77.447 kb on - strandat 77.451 kb on + strandat 77.456 kb on + strandat 77.465 kb on - strandat 77.469 kb on + strandat 77.469 kb on + strandat 77.484 kb on - strandat 77.484 kb on - strandat 77.485 kb on + strandat 77.485 kb on + strandat 77.485 kb on + strandat 77.486 kb on - strandat 77.486 kb on - strandat 77.486 kb on - strandat 77.486 kb on - strandat 77.486 kb on - strandat 77.486 kb on - strandat 77.486 kb on - strandat 77.487 kb on + strandat 77.487 kb on + strandat 77.487 kb on + strandat 77.487 kb on + strandat 77.487 kb on + strandat 77.488 kb on - strandat 77.488 kb on - strandat 77.489 kb on + strandat 77.489 kb on + strandat 77.489 kb on + strandat 77.489 kb on + strandat 77.500 kb on - strandat 77.500 kb on - strandat 77.506 kb on + strandat 77.541 kb on - strandat 77.541 kb on - strandat 77.541 kb on - strandat 77.541 kb on - strandat 77.542 kb on + strandat 77.542 kb on + strandat 77.542 kb on + strandat 77.544 kb on - strandat 77.562 kb on - strandat 77.563 kb on + strandat 77.563 kb on + strandat 77.577 kb on - strand, within MMJJ_RS00425at 77.620 kb on - strand, within MMJJ_RS00425at 77.621 kb on + strand, within MMJJ_RS00425at 77.621 kb on + strand, within MMJJ_RS00425at 77.675 kb on - strand, within MMJJ_RS00425at 77.874 kb on + strand, within MMJJ_RS00425at 77.927 kb on - strandat 77.932 kb on - strandat 77.933 kb on + strandat 77.933 kb on + strandat 77.933 kb on + strandat 77.934 kb on - strandat 77.935 kb on + strandat 77.935 kb on + strandat 77.984 kb on + strandat 78.087 kb on - strand, within MMJJ_RS00430at 78.357 kb on - strand, within MMJJ_RS00430at 78.485 kb on + strand, within MMJJ_RS00430at 78.742 kb on + strand, within MMJJ_RS00430at 78.775 kb on + strand, within MMJJ_RS00430at 78.876 kb on + strandat 78.876 kb on + strandat 78.876 kb on + strandat 78.895 kb on - strandat 78.904 kb on - strandat 78.905 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM
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76,582 + MMJJ_RS00415 0.25 -0.3
76,647 - MMJJ_RS00415 0.39 -0.5
76,647 - MMJJ_RS00415 0.39 -1.7
76,680 + MMJJ_RS00415 0.45 -3.8
76,751 - MMJJ_RS00415 0.60 -2.0
76,751 - MMJJ_RS00415 0.60 -3.5
76,763 - MMJJ_RS00415 0.63 -1.3
76,783 + MMJJ_RS00415 0.67 -2.5
76,833 + MMJJ_RS00415 0.77 -2.4
76,833 + MMJJ_RS00415 0.77 -1.4
76,836 - MMJJ_RS00415 0.78 -1.8
76,837 + MMJJ_RS00415 0.78 -2.9
76,837 + MMJJ_RS00415 0.78 -0.2
76,838 - MMJJ_RS00415 0.78 -1.5
76,839 + MMJJ_RS00415 0.78 -1.5
76,839 + MMJJ_RS00415 0.78 -3.8
76,839 + MMJJ_RS00415 0.78 -1.0
76,845 - MMJJ_RS00415 0.80 -2.3
76,903 - -2.8
76,908 + -2.0
76,908 + -1.9
76,911 - -1.4
76,982 - -3.1
76,998 + MMJJ_RS00420 0.10 -1.6
76,999 - MMJJ_RS00420 0.11 -2.9
77,030 - MMJJ_RS00420 0.19 -2.0
77,045 - MMJJ_RS00420 0.23 -1.3
77,108 - MMJJ_RS00420 0.39 -1.5
77,138 - MMJJ_RS00420 0.47 -2.0
77,138 - MMJJ_RS00420 0.47 -3.6
77,138 - MMJJ_RS00420 0.47 +0.6
77,139 + MMJJ_RS00420 0.48 +2.0
77,139 + MMJJ_RS00420 0.48 -2.4
77,199 + MMJJ_RS00420 0.63 -4.0
77,199 + MMJJ_RS00420 0.63 +0.3
77,199 + MMJJ_RS00420 0.63 -3.5
77,200 - MMJJ_RS00420 0.64 +0.2
77,222 - MMJJ_RS00420 0.69 -0.2
77,222 - MMJJ_RS00420 0.69 -2.9
77,223 + MMJJ_RS00420 0.70 -1.3
77,228 - MMJJ_RS00420 0.71 +0.8
77,229 + MMJJ_RS00420 0.71 -1.5
77,252 + MMJJ_RS00420 0.77 -3.7
77,262 + MMJJ_RS00420 0.80 +0.9
77,266 - MMJJ_RS00420 0.81 -1.6
77,367 - +1.4
77,370 - +1.4
77,370 - +0.5
77,371 + -4.5
77,372 - -1.8
77,372 - -3.7
77,373 + -3.0
77,373 + +0.2
77,374 - -3.4
77,374 - +1.2
77,375 + -3.0
77,379 + -2.4
77,427 + -0.2
77,441 - -3.0
77,441 - -4.5
77,445 - -3.5
77,446 + -3.5
77,446 + +0.8
77,446 + +0.5
77,446 + -2.5
77,446 + -3.1
77,447 - -0.9
77,451 + -3.9
77,456 + -3.2
77,465 - -4.2
77,469 + -3.4
77,469 + -2.3
77,484 - -3.9
77,484 - -2.2
77,485 + -3.1
77,485 + -0.1
77,485 + -0.7
77,486 - -2.0
77,486 - -2.4
77,486 - -2.9
77,486 - -3.5
77,486 - -3.6
77,486 - -2.9
77,486 - -1.0
77,487 + -1.9
77,487 + -2.0
77,487 + -1.9
77,487 + -1.3
77,487 + +1.8
77,488 - -1.0
77,488 - -0.2
77,489 + -1.8
77,489 + -2.9
77,489 + -3.2
77,489 + -1.9
77,500 - -3.5
77,500 - +0.3
77,506 + -3.0
77,541 - -3.2
77,541 - -2.8
77,541 - -2.4
77,541 - -3.2
77,542 + -2.7
77,542 + -3.0
77,542 + -3.7
77,544 - +0.8
77,562 - -3.2
77,563 + -4.0
77,563 + +0.5
77,577 - MMJJ_RS00425 0.13 -1.7
77,620 - MMJJ_RS00425 0.24 -1.9
77,621 + MMJJ_RS00425 0.25 -2.2
77,621 + MMJJ_RS00425 0.25 -2.8
77,675 - MMJJ_RS00425 0.38 -1.9
77,874 + MMJJ_RS00425 0.89 -3.0
77,927 - -1.0
77,932 - -0.7
77,933 + -4.7
77,933 + -4.1
77,933 + -4.2
77,934 - +0.8
77,935 + -3.1
77,935 + -1.3
77,984 + +0.3
78,087 - MMJJ_RS00430 0.15 -0.4
78,357 - MMJJ_RS00430 0.44 -2.5
78,485 + MMJJ_RS00430 0.58 -1.3
78,742 + MMJJ_RS00430 0.86 -1.2
78,775 + MMJJ_RS00430 0.90 +0.8
78,876 + +0.5
78,876 + -2.8
78,876 + -1.7
78,895 - -0.1
78,904 - -0.2
78,905 + +0.7

Or see this region's nucleotide sequence