Experiment: McCas_formate_complete Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08465 and MMJJ_RS08470 are separated by 207 nucleotides MMJJ_RS08470 and MMJJ_RS08475 are separated by 175 nucleotides MMJJ_RS08475 and MMJJ_RS08480 overlap by 11 nucleotides MMJJ_RS08480 and MMJJ_RS08485 are separated by 204 nucleotides
MMJJ_RS08465: MMJJ_RS08465 - ATP-dependent helicase, at 1,556,465 to 1,559,089
_RS08465
MMJJ_RS08470: MMJJ_RS08470 - ferredoxin family protein, at 1,559,297 to 1,559,542
_RS08470
MMJJ_RS08475: MMJJ_RS08475 - thiamine phosphate synthase, at 1,559,718 to 1,560,341
_RS08475
MMJJ_RS08480: MMJJ_RS08480 - hydroxyethylthiazole kinase, at 1,560,331 to 1,561,131
_RS08480
MMJJ_RS08485: MMJJ_RS08485 - Lrp/AsnC family transcriptional regulator, at 1,561,336 to 1,561,758
_RS08485
Position (kb)
1559
1560
1561 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1558.821 kb on - strand, within MMJJ_RS08465 at 1558.821 kb on - strand, within MMJJ_RS08465 at 1558.821 kb on - strand, within MMJJ_RS08465 at 1558.821 kb on - strand, within MMJJ_RS08465 at 1558.821 kb on - strand, within MMJJ_RS08465 at 1558.822 kb on + strand, within MMJJ_RS08465 at 1558.822 kb on + strand, within MMJJ_RS08465 at 1558.823 kb on - strand, within MMJJ_RS08465 at 1558.824 kb on + strand, within MMJJ_RS08465 at 1558.830 kb on - strand at 1558.830 kb on - strand at 1558.831 kb on + strand at 1558.835 kb on + strand at 1558.882 kb on - strand at 1558.939 kb on - strand at 1558.955 kb on - strand at 1558.993 kb on + strand at 1559.000 kb on + strand at 1559.027 kb on + strand at 1559.030 kb on - strand at 1559.060 kb on - strand at 1559.069 kb on - strand at 1559.128 kb on - strand at 1559.129 kb on + strand at 1559.129 kb on + strand at 1559.144 kb on + strand at 1559.144 kb on + strand at 1559.144 kb on + strand at 1559.164 kb on - strand at 1559.165 kb on + strand at 1559.165 kb on + strand at 1559.186 kb on - strand at 1559.186 kb on - strand at 1559.209 kb on - strand at 1559.210 kb on + strand at 1559.227 kb on + strand at 1559.230 kb on - strand at 1559.231 kb on + strand at 1559.231 kb on + strand at 1559.231 kb on + strand at 1559.231 kb on + strand at 1559.231 kb on + strand at 1559.253 kb on + strand at 1559.253 kb on + strand at 1559.258 kb on - strand at 1559.258 kb on - strand at 1559.259 kb on + strand at 1559.260 kb on - strand at 1559.260 kb on - strand at 1559.260 kb on - strand at 1559.260 kb on - strand at 1559.260 kb on - strand at 1559.260 kb on - strand at 1559.260 kb on - strand at 1559.261 kb on + strand at 1559.261 kb on + strand at 1559.261 kb on + strand at 1559.261 kb on + strand at 1559.261 kb on + strand at 1559.264 kb on - strand at 1559.264 kb on - strand at 1559.265 kb on + strand at 1559.315 kb on - strand at 1559.363 kb on - strand, within MMJJ_RS08470 at 1559.482 kb on - strand, within MMJJ_RS08470 at 1559.535 kb on + strand at 1559.542 kb on - strand at 1559.543 kb on + strand at 1559.572 kb on - strand at 1559.572 kb on - strand at 1559.588 kb on + strand at 1559.588 kb on + strand at 1559.609 kb on + strand at 1559.610 kb on - strand at 1559.611 kb on + strand at 1559.611 kb on + strand at 1559.611 kb on + strand at 1559.653 kb on - strand at 1559.695 kb on - strand at 1559.701 kb on + strand at 1559.701 kb on + strand at 1560.078 kb on + strand, within MMJJ_RS08475 at 1560.370 kb on - strand at 1560.404 kb on + strand at 1560.472 kb on - strand, within MMJJ_RS08480 at 1560.472 kb on - strand, within MMJJ_RS08480 at 1560.473 kb on + strand, within MMJJ_RS08480 at 1560.473 kb on + strand, within MMJJ_RS08480 at 1560.528 kb on - strand, within MMJJ_RS08480 at 1560.538 kb on + strand, within MMJJ_RS08480 at 1560.538 kb on + strand, within MMJJ_RS08480 at 1560.538 kb on + strand, within MMJJ_RS08480 at 1560.596 kb on - strand, within MMJJ_RS08480 at 1560.597 kb on + strand, within MMJJ_RS08480 at 1560.616 kb on - strand, within MMJJ_RS08480 at 1560.616 kb on - strand, within MMJJ_RS08480 at 1560.616 kb on - strand, within MMJJ_RS08480 at 1560.616 kb on - strand, within MMJJ_RS08480 at 1560.616 kb on - strand, within MMJJ_RS08480 at 1560.617 kb on + strand, within MMJJ_RS08480 at 1560.704 kb on - strand, within MMJJ_RS08480 at 1560.866 kb on + strand, within MMJJ_RS08480 at 1560.949 kb on - strand, within MMJJ_RS08480 at 1560.952 kb on - strand, within MMJJ_RS08480 at 1560.991 kb on - strand, within MMJJ_RS08480 at 1561.055 kb on + strand at 1561.062 kb on + strand at 1561.068 kb on - strand at 1561.120 kb on - strand at 1561.120 kb on - strand at 1561.155 kb on - strand at 1561.177 kb on - strand at 1561.177 kb on - strand at 1561.177 kb on - strand at 1561.179 kb on - strand at 1561.179 kb on - strand at 1561.179 kb on - strand at 1561.183 kb on - strand at 1561.183 kb on - strand at 1561.184 kb on + strand at 1561.233 kb on - strand at 1561.234 kb on + strand at 1561.234 kb on + strand at 1561.234 kb on + strand at 1561.238 kb on + strand at 1561.242 kb on - strand at 1561.243 kb on + strand at 1561.246 kb on - strand at 1561.247 kb on + strand at 1561.254 kb on + strand at 1561.254 kb on + strand at 1561.255 kb on - strand at 1561.260 kb on - strand at 1561.260 kb on - strand at 1561.260 kb on - strand at 1561.260 kb on - strand at 1561.260 kb on - strand at 1561.260 kb on - strand at 1561.260 kb on - strand at 1561.261 kb on + strand at 1561.261 kb on + strand at 1561.261 kb on + strand at 1561.261 kb on + strand at 1561.262 kb on - strand at 1561.262 kb on - strand at 1561.262 kb on - strand at 1561.263 kb on + strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.264 kb on - strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.265 kb on + strand at 1561.266 kb on - strand at 1561.266 kb on - strand at 1561.266 kb on - strand at 1561.266 kb on - strand at 1561.267 kb on + strand at 1561.267 kb on + strand at 1561.267 kb on + strand at 1561.267 kb on + strand at 1561.267 kb on + strand at 1561.267 kb on + strand at 1561.268 kb on - strand at 1561.268 kb on - strand at 1561.268 kb on - strand at 1561.268 kb on - strand at 1561.268 kb on - strand at 1561.268 kb on - strand at 1561.299 kb on - strand at 1561.311 kb on - strand at 1561.312 kb on + strand at 1561.315 kb on + strand at 1561.316 kb on - strand at 1561.316 kb on - strand at 1561.316 kb on - strand at 1561.316 kb on - strand at 1561.317 kb on + strand at 1561.317 kb on + strand at 1561.317 kb on + strand at 1561.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Sulfaquinoxaline 0.1 mM remove 1,558,821 - MMJJ_RS08465 0.90 -0.4 1,558,821 - MMJJ_RS08465 0.90 -0.8 1,558,821 - MMJJ_RS08465 0.90 +1.6 1,558,821 - MMJJ_RS08465 0.90 -0.6 1,558,821 - MMJJ_RS08465 0.90 +0.7 1,558,822 + MMJJ_RS08465 0.90 -0.6 1,558,822 + MMJJ_RS08465 0.90 -4.1 1,558,823 - MMJJ_RS08465 0.90 -0.4 1,558,824 + MMJJ_RS08465 0.90 +0.3 1,558,830 - +0.1 1,558,830 - +1.5 1,558,831 + +0.3 1,558,835 + -1.8 1,558,882 - -0.7 1,558,939 - +0.7 1,558,955 - +0.1 1,558,993 + +0.5 1,559,000 + -1.7 1,559,027 + +1.0 1,559,030 - +0.2 1,559,060 - +0.5 1,559,069 - -0.9 1,559,128 - -1.1 1,559,129 + -0.2 1,559,129 + -1.2 1,559,144 + +1.0 1,559,144 + -4.7 1,559,144 + +2.3 1,559,164 - -1.9 1,559,165 + -0.3 1,559,165 + -2.6 1,559,186 - +1.0 1,559,186 - -1.5 1,559,209 - -0.2 1,559,210 + +0.1 1,559,227 + -3.3 1,559,230 - -3.2 1,559,231 + +1.0 1,559,231 + +1.8 1,559,231 + -1.2 1,559,231 + -1.6 1,559,231 + -1.1 1,559,253 + -0.1 1,559,253 + +1.0 1,559,258 - -1.3 1,559,258 - -2.0 1,559,259 + +1.1 1,559,260 - +0.8 1,559,260 - +0.2 1,559,260 - +1.6 1,559,260 - +0.1 1,559,260 - -1.2 1,559,260 - +1.0 1,559,260 - +0.6 1,559,261 + +1.2 1,559,261 + +1.2 1,559,261 + +2.1 1,559,261 + +0.8 1,559,261 + -0.8 1,559,264 - +2.1 1,559,264 - +0.2 1,559,265 + -0.2 1,559,315 - -0.7 1,559,363 - MMJJ_RS08470 0.27 +0.1 1,559,482 - MMJJ_RS08470 0.75 -0.8 1,559,535 + -0.7 1,559,542 - -1.4 1,559,543 + -4.6 1,559,572 - -2.6 1,559,572 - -1.5 1,559,588 + -3.5 1,559,588 + -3.0 1,559,609 + -0.4 1,559,610 - -3.1 1,559,611 + -0.3 1,559,611 + -0.5 1,559,611 + -0.6 1,559,653 - +1.6 1,559,695 - -0.1 1,559,701 + +0.9 1,559,701 + -3.2 1,560,078 + MMJJ_RS08475 0.58 -0.4 1,560,370 - -0.3 1,560,404 + -1.4 1,560,472 - MMJJ_RS08480 0.18 +1.1 1,560,472 - MMJJ_RS08480 0.18 -0.5 1,560,473 + MMJJ_RS08480 0.18 -1.4 1,560,473 + MMJJ_RS08480 0.18 -2.9 1,560,528 - MMJJ_RS08480 0.25 -1.0 1,560,538 + MMJJ_RS08480 0.26 -3.0 1,560,538 + MMJJ_RS08480 0.26 +0.1 1,560,538 + MMJJ_RS08480 0.26 -1.7 1,560,596 - MMJJ_RS08480 0.33 -1.4 1,560,597 + MMJJ_RS08480 0.33 -0.2 1,560,616 - MMJJ_RS08480 0.36 -1.3 1,560,616 - MMJJ_RS08480 0.36 -2.3 1,560,616 - MMJJ_RS08480 0.36 +1.2 1,560,616 - MMJJ_RS08480 0.36 +2.2 1,560,616 - MMJJ_RS08480 0.36 -3.4 1,560,617 + MMJJ_RS08480 0.36 -0.2 1,560,704 - MMJJ_RS08480 0.47 +1.6 1,560,866 + MMJJ_RS08480 0.67 -1.3 1,560,949 - MMJJ_RS08480 0.77 -4.2 1,560,952 - MMJJ_RS08480 0.78 -0.2 1,560,991 - MMJJ_RS08480 0.82 -1.8 1,561,055 + -3.0 1,561,062 + +1.1 1,561,068 - +0.8 1,561,120 - -1.5 1,561,120 - +1.0 1,561,155 - -0.4 1,561,177 - +0.9 1,561,177 - -0.4 1,561,177 - -1.3 1,561,179 - -2.9 1,561,179 - -0.5 1,561,179 - -2.4 1,561,183 - +2.9 1,561,183 - +0.6 1,561,184 + -0.3 1,561,233 - -0.4 1,561,234 + -0.4 1,561,234 + +0.4 1,561,234 + +0.8 1,561,238 + -1.6 1,561,242 - +0.0 1,561,243 + -0.2 1,561,246 - -0.2 1,561,247 + -4.0 1,561,254 + -0.9 1,561,254 + -1.9 1,561,255 - -0.1 1,561,260 - -0.9 1,561,260 - -3.0 1,561,260 - -2.7 1,561,260 - -4.7 1,561,260 - -1.4 1,561,260 - -3.3 1,561,260 - -0.4 1,561,261 + -1.6 1,561,261 + +0.3 1,561,261 + +0.7 1,561,261 + +1.1 1,561,262 - +1.1 1,561,262 - -0.4 1,561,262 - -3.0 1,561,263 + +0.1 1,561,264 - +0.0 1,561,264 - +0.4 1,561,264 - -2.4 1,561,264 - -3.8 1,561,264 - +2.4 1,561,264 - -2.8 1,561,264 - -0.4 1,561,264 - +2.1 1,561,264 - -0.7 1,561,264 - -1.0 1,561,264 - +3.1 1,561,264 - +0.3 1,561,264 - +0.9 1,561,264 - +1.0 1,561,265 + -4.2 1,561,265 + -1.3 1,561,265 + +0.7 1,561,265 + +0.1 1,561,265 + +0.6 1,561,265 + -0.5 1,561,265 + +0.6 1,561,265 + +1.5 1,561,265 + +0.1 1,561,265 + -1.7 1,561,265 + -1.0 1,561,266 - -0.2 1,561,266 - -0.3 1,561,266 - +0.5 1,561,266 - +0.7 1,561,267 + +0.9 1,561,267 + +0.2 1,561,267 + -2.2 1,561,267 + +2.1 1,561,267 + +1.8 1,561,267 + +1.0 1,561,268 - +1.3 1,561,268 - -0.1 1,561,268 - -1.6 1,561,268 - +2.1 1,561,268 - +1.2 1,561,268 - +0.7 1,561,299 - -0.2 1,561,311 - -1.1 1,561,312 + -2.4 1,561,315 + -0.3 1,561,316 - -2.6 1,561,316 - -0.3 1,561,316 - -1.6 1,561,316 - -1.0 1,561,317 + -3.2 1,561,317 + -2.2 1,561,317 + +2.9 1,561,320 - -0.2
Or see this region's nucleotide sequence