Experiment: McCas_formate_complete Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03555 and MMJJ_RS03560 are separated by 45 nucleotides MMJJ_RS03560 and MMJJ_RS03565 are separated by 212 nucleotides MMJJ_RS03565 and MMJJ_RS03570 are separated by 27 nucleotides MMJJ_RS03570 and MMJJ_RS03575 are separated by 110 nucleotides
MMJJ_RS03555: MMJJ_RS03555 - acetyl-CoA carboxylase biotin carboxylase subunit, at 664,424 to 665,929
_RS03555
MMJJ_RS03560: MMJJ_RS03560 - sodium-extruding oxaloacetate decarboxylase subunit alpha, at 665,975 to 667,684
_RS03560
MMJJ_RS03565: MMJJ_RS03565 - tRNA-Glu, at 667,897 to 667,971
_RS03565
MMJJ_RS03570: MMJJ_RS03570 - tRNA-Arg, at 667,999 to 668,076
_RS03570
MMJJ_RS03575: MMJJ_RS03575 - NUDIX hydrolase, at 668,187 to 668,702
_RS03575
Position (kb) 665
666
667
668 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 665.807 kb on + strand at 665.963 kb on - strand at 666.085 kb on - strand at 666.200 kb on - strand at 667.257 kb on - strand at 667.782 kb on - strand at 667.841 kb on - strand at 667.841 kb on - strand at 667.842 kb on + strand at 667.842 kb on + strand at 667.980 kb on + strand at 668.149 kb on + strand at 668.165 kb on - strand at 668.166 kb on + strand at 668.166 kb on + strand at 668.173 kb on + strand at 668.174 kb on - strand at 668.179 kb on - strand at 668.179 kb on - strand at 668.181 kb on - strand at 668.182 kb on + strand at 668.182 kb on + strand at 668.182 kb on + strand at 668.196 kb on - strand at 668.196 kb on - strand at 668.197 kb on + strand at 668.197 kb on + strand at 668.198 kb on - strand at 668.198 kb on - strand at 668.198 kb on - strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.201 kb on + strand at 668.217 kb on - strand at 668.218 kb on + strand at 668.256 kb on - strand, within MMJJ_RS03575 at 668.270 kb on - strand, within MMJJ_RS03575 at 668.270 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.307 kb on - strand, within MMJJ_RS03575 at 668.348 kb on - strand, within MMJJ_RS03575 at 668.390 kb on - strand, within MMJJ_RS03575 at 668.409 kb on + strand, within MMJJ_RS03575 at 668.487 kb on - strand, within MMJJ_RS03575 at 668.548 kb on - strand, within MMJJ_RS03575 at 668.549 kb on + strand, within MMJJ_RS03575 at 668.552 kb on - strand, within MMJJ_RS03575 at 668.571 kb on - strand, within MMJJ_RS03575 at 668.572 kb on + strand, within MMJJ_RS03575 at 668.649 kb on - strand, within MMJJ_RS03575
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Sulfaquinoxaline 0.1 mM remove 665,807 + -1.6 665,963 - +0.3 666,085 - +0.7 666,200 - +1.2 667,257 - +0.7 667,782 - -0.3 667,841 - -0.5 667,841 - +1.2 667,842 + +0.9 667,842 + +0.7 667,980 + +0.2 668,149 + +0.8 668,165 - +0.2 668,166 + -0.7 668,166 + -1.8 668,173 + -1.8 668,174 - +0.5 668,179 - +1.0 668,179 - +0.5 668,181 - -1.0 668,182 + -2.5 668,182 + +0.9 668,182 + +0.2 668,196 - +1.0 668,196 - -0.2 668,197 + +1.0 668,197 + -1.8 668,198 - -1.1 668,198 - -1.2 668,198 - +0.1 668,199 + -1.3 668,199 + +0.8 668,199 + -0.8 668,199 + -3.1 668,199 + +1.0 668,199 + -0.3 668,199 + -2.5 668,199 + -0.8 668,201 + +1.4 668,217 - +1.9 668,218 + +1.4 668,256 - MMJJ_RS03575 0.13 -0.2 668,270 - MMJJ_RS03575 0.16 +1.5 668,270 - MMJJ_RS03575 0.16 -0.8 668,274 - MMJJ_RS03575 0.17 -2.6 668,274 - MMJJ_RS03575 0.17 +1.9 668,274 - MMJJ_RS03575 0.17 +2.4 668,274 - MMJJ_RS03575 0.17 -4.1 668,307 - MMJJ_RS03575 0.23 +0.4 668,348 - MMJJ_RS03575 0.31 -0.3 668,390 - MMJJ_RS03575 0.39 +0.5 668,409 + MMJJ_RS03575 0.43 +1.8 668,487 - MMJJ_RS03575 0.58 +1.5 668,548 - MMJJ_RS03575 0.70 +1.1 668,549 + MMJJ_RS03575 0.70 +2.0 668,552 - MMJJ_RS03575 0.71 +0.4 668,571 - MMJJ_RS03575 0.74 -3.9 668,572 + MMJJ_RS03575 0.75 +0.1 668,649 - MMJJ_RS03575 0.90 +1.5
Or see this region's nucleotide sequence