Experiment: McCas_formate_complete Fluorophosphate 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06060 and MMJJ_RS06065 are separated by 86 nucleotides MMJJ_RS06065 and MMJJ_RS06070 are separated by 69 nucleotides MMJJ_RS06070 and MMJJ_RS06075 are separated by 35 nucleotides MMJJ_RS06075 and MMJJ_RS06080 are separated by 111 nucleotides
MMJJ_RS06060: MMJJ_RS06060 - aspartate--tRNA(Asn) ligase, at 1,125,780 to 1,127,096
_RS06060
MMJJ_RS06065: MMJJ_RS06065 - metal-sulfur cluster assembly factor, at 1,127,183 to 1,127,482
_RS06065
MMJJ_RS06070: MMJJ_RS06070 - histidine--tRNA ligase, at 1,127,552 to 1,128,802
_RS06070
MMJJ_RS06075: MMJJ_RS06075 - DNA-binding protein Alba, at 1,128,838 to 1,129,107
_RS06075
MMJJ_RS06080: MMJJ_RS06080 - serine/threonine protein kinase, at 1,129,219 to 1,129,896
_RS06080
Position (kb)
1127
1128
1129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1127.119 kb on - strand at 1127.122 kb on - strand at 1127.122 kb on - strand at 1127.125 kb on - strand at 1127.133 kb on - strand at 1127.192 kb on + strand at 1127.223 kb on - strand, within MMJJ_RS06065 at 1127.266 kb on - strand, within MMJJ_RS06065 at 1127.314 kb on + strand, within MMJJ_RS06065 at 1127.443 kb on + strand at 1127.504 kb on - strand at 1127.506 kb on - strand at 1127.507 kb on + strand at 1128.351 kb on + strand, within MMJJ_RS06070 at 1128.353 kb on - strand, within MMJJ_RS06070 at 1128.787 kb on - strand at 1128.787 kb on - strand at 1128.844 kb on + strand at 1128.868 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.898 kb on - strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.899 kb on + strand, within MMJJ_RS06075 at 1128.900 kb on - strand, within MMJJ_RS06075 at 1128.901 kb on + strand, within MMJJ_RS06075 at 1129.009 kb on - strand, within MMJJ_RS06075 at 1129.039 kb on + strand, within MMJJ_RS06075 at 1129.048 kb on + strand, within MMJJ_RS06075 at 1129.064 kb on - strand, within MMJJ_RS06075 at 1129.064 kb on - strand, within MMJJ_RS06075 at 1129.073 kb on + strand, within MMJJ_RS06075 at 1129.091 kb on - strand at 1129.092 kb on + strand at 1129.098 kb on - strand at 1129.131 kb on - strand at 1129.132 kb on + strand at 1129.145 kb on - strand at 1129.145 kb on - strand at 1129.145 kb on - strand at 1129.145 kb on - strand at 1129.146 kb on + strand at 1129.146 kb on + strand at 1129.147 kb on - strand at 1129.147 kb on - strand at 1129.147 kb on - strand at 1129.148 kb on + strand at 1129.148 kb on + strand at 1129.149 kb on - strand at 1129.149 kb on - strand at 1129.149 kb on - strand at 1129.150 kb on + strand at 1129.162 kb on + strand at 1129.195 kb on + strand at 1129.195 kb on + strand at 1129.257 kb on - strand at 1129.312 kb on - strand, within MMJJ_RS06080 at 1129.330 kb on - strand, within MMJJ_RS06080 at 1129.330 kb on - strand, within MMJJ_RS06080 at 1129.331 kb on + strand, within MMJJ_RS06080 at 1129.331 kb on + strand, within MMJJ_RS06080 at 1129.356 kb on + strand, within MMJJ_RS06080 at 1129.382 kb on + strand, within MMJJ_RS06080 at 1129.405 kb on + strand, within MMJJ_RS06080 at 1129.546 kb on - strand, within MMJJ_RS06080 at 1129.547 kb on + strand, within MMJJ_RS06080 at 1129.606 kb on - strand, within MMJJ_RS06080 at 1129.606 kb on - strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.607 kb on + strand, within MMJJ_RS06080 at 1129.610 kb on - strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.611 kb on + strand, within MMJJ_RS06080 at 1129.644 kb on + strand, within MMJJ_RS06080 at 1129.692 kb on - strand, within MMJJ_RS06080 at 1129.693 kb on + strand, within MMJJ_RS06080 at 1129.714 kb on - strand, within MMJJ_RS06080 at 1129.722 kb on + strand, within MMJJ_RS06080 at 1129.723 kb on - strand, within MMJJ_RS06080 at 1129.779 kb on + strand, within MMJJ_RS06080 at 1129.782 kb on + strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.795 kb on - strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.798 kb on + strand, within MMJJ_RS06080 at 1129.799 kb on - strand, within MMJJ_RS06080 at 1129.800 kb on + strand, within MMJJ_RS06080 at 1129.800 kb on + strand, within MMJJ_RS06080
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Fluorophosphate 31.25 mM remove 1,127,119 - +0.9 1,127,122 - -1.0 1,127,122 - -0.3 1,127,125 - +0.6 1,127,133 - +0.3 1,127,192 + +1.9 1,127,223 - MMJJ_RS06065 0.13 -1.4 1,127,266 - MMJJ_RS06065 0.28 -0.7 1,127,314 + MMJJ_RS06065 0.44 -0.4 1,127,443 + +1.3 1,127,504 - +0.9 1,127,506 - +1.0 1,127,507 + +0.9 1,128,351 + MMJJ_RS06070 0.64 +0.6 1,128,353 - MMJJ_RS06070 0.64 +0.3 1,128,787 - -1.2 1,128,787 - -1.1 1,128,844 + +1.1 1,128,868 - MMJJ_RS06075 0.11 +0.1 1,128,898 - MMJJ_RS06075 0.22 +0.3 1,128,898 - MMJJ_RS06075 0.22 -1.2 1,128,898 - MMJJ_RS06075 0.22 +0.9 1,128,898 - MMJJ_RS06075 0.22 +2.2 1,128,898 - MMJJ_RS06075 0.22 +0.0 1,128,898 - MMJJ_RS06075 0.22 -1.0 1,128,898 - MMJJ_RS06075 0.22 -1.9 1,128,898 - MMJJ_RS06075 0.22 +0.2 1,128,898 - MMJJ_RS06075 0.22 +0.8 1,128,898 - MMJJ_RS06075 0.22 +1.4 1,128,899 + MMJJ_RS06075 0.23 -3.1 1,128,899 + MMJJ_RS06075 0.23 +0.6 1,128,899 + MMJJ_RS06075 0.23 +0.7 1,128,899 + MMJJ_RS06075 0.23 -0.4 1,128,899 + MMJJ_RS06075 0.23 +0.2 1,128,899 + MMJJ_RS06075 0.23 -1.7 1,128,899 + MMJJ_RS06075 0.23 -2.3 1,128,899 + MMJJ_RS06075 0.23 +0.1 1,128,900 - MMJJ_RS06075 0.23 -1.5 1,128,901 + MMJJ_RS06075 0.23 +0.2 1,129,009 - MMJJ_RS06075 0.63 -0.9 1,129,039 + MMJJ_RS06075 0.74 -1.4 1,129,048 + MMJJ_RS06075 0.78 +0.6 1,129,064 - MMJJ_RS06075 0.84 +0.6 1,129,064 - MMJJ_RS06075 0.84 -1.4 1,129,073 + MMJJ_RS06075 0.87 +1.7 1,129,091 - -1.8 1,129,092 + +1.1 1,129,098 - +1.7 1,129,131 - +0.5 1,129,132 + -1.1 1,129,145 - -1.6 1,129,145 - +0.7 1,129,145 - -2.2 1,129,145 - -3.0 1,129,146 + +0.0 1,129,146 + +2.7 1,129,147 - -1.5 1,129,147 - +1.1 1,129,147 - -0.6 1,129,148 + -2.7 1,129,148 + +3.7 1,129,149 - -2.4 1,129,149 - -0.5 1,129,149 - -3.4 1,129,150 + -0.2 1,129,162 + +2.1 1,129,195 + -2.3 1,129,195 + +0.7 1,129,257 - -0.6 1,129,312 - MMJJ_RS06080 0.14 -3.4 1,129,330 - MMJJ_RS06080 0.16 +2.6 1,129,330 - MMJJ_RS06080 0.16 -1.8 1,129,331 + MMJJ_RS06080 0.17 -0.7 1,129,331 + MMJJ_RS06080 0.17 +1.7 1,129,356 + MMJJ_RS06080 0.20 +1.2 1,129,382 + MMJJ_RS06080 0.24 +1.1 1,129,405 + MMJJ_RS06080 0.27 -1.3 1,129,546 - MMJJ_RS06080 0.48 -1.2 1,129,547 + MMJJ_RS06080 0.48 -0.8 1,129,606 - MMJJ_RS06080 0.57 +0.7 1,129,606 - MMJJ_RS06080 0.57 -2.2 1,129,607 + MMJJ_RS06080 0.57 +0.0 1,129,607 + MMJJ_RS06080 0.57 +1.3 1,129,607 + MMJJ_RS06080 0.57 +4.5 1,129,607 + MMJJ_RS06080 0.57 +0.6 1,129,607 + MMJJ_RS06080 0.57 -0.4 1,129,607 + MMJJ_RS06080 0.57 -2.9 1,129,607 + MMJJ_RS06080 0.57 +0.7 1,129,610 - MMJJ_RS06080 0.58 +2.7 1,129,611 + MMJJ_RS06080 0.58 -1.9 1,129,611 + MMJJ_RS06080 0.58 -3.0 1,129,611 + MMJJ_RS06080 0.58 +1.2 1,129,611 + MMJJ_RS06080 0.58 +0.8 1,129,644 + MMJJ_RS06080 0.63 +0.7 1,129,692 - MMJJ_RS06080 0.70 -2.0 1,129,693 + MMJJ_RS06080 0.70 -0.3 1,129,714 - MMJJ_RS06080 0.73 +1.5 1,129,722 + MMJJ_RS06080 0.74 -1.6 1,129,723 - MMJJ_RS06080 0.74 +0.1 1,129,779 + MMJJ_RS06080 0.83 -0.5 1,129,782 + MMJJ_RS06080 0.83 -1.1 1,129,795 - MMJJ_RS06080 0.85 +1.0 1,129,795 - MMJJ_RS06080 0.85 +0.8 1,129,795 - MMJJ_RS06080 0.85 -0.7 1,129,795 - MMJJ_RS06080 0.85 -1.7 1,129,798 + MMJJ_RS06080 0.85 -1.2 1,129,798 + MMJJ_RS06080 0.85 +2.7 1,129,798 + MMJJ_RS06080 0.85 +1.6 1,129,798 + MMJJ_RS06080 0.85 +2.3 1,129,799 - MMJJ_RS06080 0.86 +0.9 1,129,800 + MMJJ_RS06080 0.86 -0.8 1,129,800 + MMJJ_RS06080 0.86 +1.2
Or see this region's nucleotide sequence