Experiment: McCas_formate_complete Fluorophosphate 31.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02155 and MMJJ_RS02160 are separated by 77 nucleotides MMJJ_RS02160 and MMJJ_RS02165 are separated by 34 nucleotides MMJJ_RS02165 and MMJJ_RS02170 are separated by 169 nucleotides MMJJ_RS02170 and MMJJ_RS02175 are separated by 9 nucleotides
MMJJ_RS02155: MMJJ_RS02155 - DMT family transporter, at 409,412 to 410,302
_RS02155
MMJJ_RS02160: MMJJ_RS02160 - amino acid ABC transporter permease, at 410,380 to 411,039
_RS02160
MMJJ_RS02165: MMJJ_RS02165 - ABC transporter substrate-binding protein, at 411,074 to 411,868
_RS02165
MMJJ_RS02170: MMJJ_RS02170 - 2-thiouracil desulfurase family protein, at 412,038 to 412,586
_RS02170
MMJJ_RS02175: MMJJ_RS02175 - imidazoleglycerol-phosphate dehydratase HisB, at 412,596 to 413,168
_RS02175
Position (kb)
411
412 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 410.086 kb on + strand, within MMJJ_RS02155 at 410.150 kb on - strand, within MMJJ_RS02155 at 410.151 kb on + strand, within MMJJ_RS02155 at 410.275 kb on - strand at 410.276 kb on + strand at 410.305 kb on - strand at 410.331 kb on - strand at 410.351 kb on - strand at 410.352 kb on + strand at 410.352 kb on + strand at 410.352 kb on + strand at 410.355 kb on - strand at 410.356 kb on + strand at 410.356 kb on + strand at 410.370 kb on - strand at 410.371 kb on + strand at 410.374 kb on - strand at 410.438 kb on + strand at 410.457 kb on + strand, within MMJJ_RS02160 at 410.460 kb on - strand, within MMJJ_RS02160 at 410.460 kb on - strand, within MMJJ_RS02160 at 410.460 kb on - strand, within MMJJ_RS02160 at 410.461 kb on + strand, within MMJJ_RS02160 at 410.461 kb on + strand, within MMJJ_RS02160 at 410.474 kb on + strand, within MMJJ_RS02160 at 410.477 kb on - strand, within MMJJ_RS02160 at 410.477 kb on - strand, within MMJJ_RS02160 at 410.478 kb on + strand, within MMJJ_RS02160 at 410.508 kb on + strand, within MMJJ_RS02160 at 410.513 kb on + strand, within MMJJ_RS02160 at 410.555 kb on + strand, within MMJJ_RS02160 at 410.593 kb on - strand, within MMJJ_RS02160 at 410.611 kb on - strand, within MMJJ_RS02160 at 410.637 kb on - strand, within MMJJ_RS02160 at 410.638 kb on + strand, within MMJJ_RS02160 at 410.639 kb on - strand, within MMJJ_RS02160 at 410.646 kb on - strand, within MMJJ_RS02160 at 410.737 kb on + strand, within MMJJ_RS02160 at 410.794 kb on + strand, within MMJJ_RS02160 at 410.819 kb on - strand, within MMJJ_RS02160 at 410.820 kb on + strand, within MMJJ_RS02160 at 410.870 kb on - strand, within MMJJ_RS02160 at 410.871 kb on + strand, within MMJJ_RS02160 at 410.871 kb on + strand, within MMJJ_RS02160 at 410.878 kb on - strand, within MMJJ_RS02160 at 410.884 kb on - strand, within MMJJ_RS02160 at 410.885 kb on + strand, within MMJJ_RS02160 at 411.055 kb on + strand at 411.080 kb on - strand at 411.081 kb on + strand at 411.081 kb on + strand at 411.161 kb on - strand, within MMJJ_RS02165 at 411.162 kb on + strand, within MMJJ_RS02165 at 411.191 kb on - strand, within MMJJ_RS02165 at 411.260 kb on - strand, within MMJJ_RS02165 at 411.261 kb on + strand, within MMJJ_RS02165 at 411.261 kb on + strand, within MMJJ_RS02165 at 411.264 kb on - strand, within MMJJ_RS02165 at 411.264 kb on - strand, within MMJJ_RS02165 at 411.264 kb on - strand, within MMJJ_RS02165 at 411.265 kb on + strand, within MMJJ_RS02165 at 411.265 kb on + strand, within MMJJ_RS02165 at 411.265 kb on + strand, within MMJJ_RS02165 at 411.268 kb on - strand, within MMJJ_RS02165 at 411.268 kb on - strand, within MMJJ_RS02165 at 411.268 kb on - strand, within MMJJ_RS02165 at 411.331 kb on + strand, within MMJJ_RS02165 at 411.335 kb on + strand, within MMJJ_RS02165 at 411.336 kb on - strand, within MMJJ_RS02165 at 411.401 kb on - strand, within MMJJ_RS02165 at 411.411 kb on + strand, within MMJJ_RS02165 at 411.422 kb on - strand, within MMJJ_RS02165 at 411.471 kb on + strand, within MMJJ_RS02165 at 411.471 kb on + strand, within MMJJ_RS02165 at 411.549 kb on + strand, within MMJJ_RS02165 at 411.558 kb on - strand, within MMJJ_RS02165 at 411.559 kb on + strand, within MMJJ_RS02165 at 411.656 kb on - strand, within MMJJ_RS02165 at 411.657 kb on + strand, within MMJJ_RS02165 at 411.657 kb on + strand, within MMJJ_RS02165 at 411.735 kb on + strand, within MMJJ_RS02165 at 411.854 kb on - strand at 411.860 kb on + strand at 411.916 kb on - strand at 411.991 kb on + strand at 412.039 kb on - strand at 412.110 kb on - strand, within MMJJ_RS02170 at 412.149 kb on + strand, within MMJJ_RS02170 at 412.211 kb on - strand, within MMJJ_RS02170 at 412.211 kb on - strand, within MMJJ_RS02170 at 412.212 kb on + strand, within MMJJ_RS02170 at 412.212 kb on + strand, within MMJJ_RS02170 at 412.285 kb on + strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.287 kb on - strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.288 kb on + strand, within MMJJ_RS02170 at 412.294 kb on + strand, within MMJJ_RS02170 at 412.311 kb on - strand, within MMJJ_RS02170 at 412.314 kb on + strand, within MMJJ_RS02170 at 412.315 kb on - strand, within MMJJ_RS02170 at 412.316 kb on + strand, within MMJJ_RS02170 at 412.316 kb on + strand, within MMJJ_RS02170 at 412.333 kb on + strand, within MMJJ_RS02170 at 412.348 kb on + strand, within MMJJ_RS02170 at 412.396 kb on + strand, within MMJJ_RS02170 at 412.396 kb on + strand, within MMJJ_RS02170 at 412.413 kb on - strand, within MMJJ_RS02170 at 412.473 kb on - strand, within MMJJ_RS02170 at 412.478 kb on + strand, within MMJJ_RS02170 at 412.495 kb on + strand, within MMJJ_RS02170 at 412.543 kb on + strand at 412.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Fluorophosphate 31.25 mM remove 410,086 + MMJJ_RS02155 0.76 +0.4 410,150 - MMJJ_RS02155 0.83 -1.2 410,151 + MMJJ_RS02155 0.83 -0.3 410,275 - +1.3 410,276 + -1.8 410,305 - -1.0 410,331 - +0.7 410,351 - -2.8 410,352 + -0.8 410,352 + -0.6 410,352 + -0.0 410,355 - +0.8 410,356 + -0.6 410,356 + -0.8 410,370 - +1.2 410,371 + +2.3 410,374 - +2.3 410,438 + -1.2 410,457 + MMJJ_RS02160 0.12 -1.7 410,460 - MMJJ_RS02160 0.12 +0.2 410,460 - MMJJ_RS02160 0.12 -0.7 410,460 - MMJJ_RS02160 0.12 -1.1 410,461 + MMJJ_RS02160 0.12 -2.9 410,461 + MMJJ_RS02160 0.12 +2.4 410,474 + MMJJ_RS02160 0.14 -1.5 410,477 - MMJJ_RS02160 0.15 +0.1 410,477 - MMJJ_RS02160 0.15 +2.3 410,478 + MMJJ_RS02160 0.15 +0.3 410,508 + MMJJ_RS02160 0.19 -0.9 410,513 + MMJJ_RS02160 0.20 +0.6 410,555 + MMJJ_RS02160 0.27 +0.9 410,593 - MMJJ_RS02160 0.32 -1.4 410,611 - MMJJ_RS02160 0.35 +0.8 410,637 - MMJJ_RS02160 0.39 +0.9 410,638 + MMJJ_RS02160 0.39 -1.4 410,639 - MMJJ_RS02160 0.39 -0.7 410,646 - MMJJ_RS02160 0.40 +1.9 410,737 + MMJJ_RS02160 0.54 -0.5 410,794 + MMJJ_RS02160 0.63 -0.1 410,819 - MMJJ_RS02160 0.67 -1.3 410,820 + MMJJ_RS02160 0.67 +1.6 410,870 - MMJJ_RS02160 0.74 -0.4 410,871 + MMJJ_RS02160 0.74 -2.3 410,871 + MMJJ_RS02160 0.74 -1.3 410,878 - MMJJ_RS02160 0.75 +2.6 410,884 - MMJJ_RS02160 0.76 -1.3 410,885 + MMJJ_RS02160 0.77 +2.1 411,055 + +3.0 411,080 - +0.9 411,081 + -1.5 411,081 + -0.8 411,161 - MMJJ_RS02165 0.11 -2.5 411,162 + MMJJ_RS02165 0.11 -3.5 411,191 - MMJJ_RS02165 0.15 -1.3 411,260 - MMJJ_RS02165 0.23 +1.1 411,261 + MMJJ_RS02165 0.24 +1.2 411,261 + MMJJ_RS02165 0.24 +0.2 411,264 - MMJJ_RS02165 0.24 +0.2 411,264 - MMJJ_RS02165 0.24 -0.4 411,264 - MMJJ_RS02165 0.24 +2.0 411,265 + MMJJ_RS02165 0.24 +0.1 411,265 + MMJJ_RS02165 0.24 +0.4 411,265 + MMJJ_RS02165 0.24 +2.1 411,268 - MMJJ_RS02165 0.24 +4.0 411,268 - MMJJ_RS02165 0.24 -3.9 411,268 - MMJJ_RS02165 0.24 -2.1 411,331 + MMJJ_RS02165 0.32 -1.3 411,335 + MMJJ_RS02165 0.33 -1.4 411,336 - MMJJ_RS02165 0.33 -0.4 411,401 - MMJJ_RS02165 0.41 +2.1 411,411 + MMJJ_RS02165 0.42 +0.7 411,422 - MMJJ_RS02165 0.44 +1.5 411,471 + MMJJ_RS02165 0.50 -0.6 411,471 + MMJJ_RS02165 0.50 +2.4 411,549 + MMJJ_RS02165 0.60 +0.5 411,558 - MMJJ_RS02165 0.61 +1.4 411,559 + MMJJ_RS02165 0.61 -3.5 411,656 - MMJJ_RS02165 0.73 -0.5 411,657 + MMJJ_RS02165 0.73 +1.9 411,657 + MMJJ_RS02165 0.73 +2.0 411,735 + MMJJ_RS02165 0.83 +2.5 411,854 - +1.4 411,860 + -2.1 411,916 - +0.9 411,991 + +1.8 412,039 - +1.4 412,110 - MMJJ_RS02170 0.13 +0.3 412,149 + MMJJ_RS02170 0.20 -1.4 412,211 - MMJJ_RS02170 0.32 -1.8 412,211 - MMJJ_RS02170 0.32 -1.0 412,212 + MMJJ_RS02170 0.32 -1.8 412,212 + MMJJ_RS02170 0.32 +2.1 412,285 + MMJJ_RS02170 0.45 -1.4 412,287 - MMJJ_RS02170 0.45 -2.2 412,287 - MMJJ_RS02170 0.45 -1.3 412,287 - MMJJ_RS02170 0.45 -0.9 412,288 + MMJJ_RS02170 0.46 +0.6 412,288 + MMJJ_RS02170 0.46 +1.3 412,288 + MMJJ_RS02170 0.46 -0.6 412,294 + MMJJ_RS02170 0.47 +0.7 412,311 - MMJJ_RS02170 0.50 +0.9 412,314 + MMJJ_RS02170 0.50 -2.2 412,315 - MMJJ_RS02170 0.50 +6.5 412,316 + MMJJ_RS02170 0.51 -0.7 412,316 + MMJJ_RS02170 0.51 +1.1 412,333 + MMJJ_RS02170 0.54 -0.7 412,348 + MMJJ_RS02170 0.56 +1.7 412,396 + MMJJ_RS02170 0.65 -1.3 412,396 + MMJJ_RS02170 0.65 +1.1 412,413 - MMJJ_RS02170 0.68 -2.1 412,473 - MMJJ_RS02170 0.79 -1.8 412,478 + MMJJ_RS02170 0.80 -1.3 412,495 + MMJJ_RS02170 0.83 +1.8 412,543 + -1.2 412,717 + +0.7
Or see this region's nucleotide sequence