Experiment: McCas_formate_complete Spiramycin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03155 and MMJJ_RS03160 are separated by 304 nucleotides MMJJ_RS03160 and MMJJ_RS03165 are separated by 18 nucleotides MMJJ_RS03165 and MMJJ_RS03170 are separated by 37 nucleotides
MMJJ_RS03155: MMJJ_RS03155 - hypothetical protein, at 595,568 to 596,653
_RS03155
MMJJ_RS03160: MMJJ_RS03160 - winged helix-turn-helix transcriptional regulator, at 596,958 to 598,319
_RS03160
MMJJ_RS03165: MMJJ_RS03165 - methionine synthase, at 598,338 to 599,276
_RS03165
MMJJ_RS03170: MMJJ_RS03170 - DUF5612 domain-containing protein, at 599,314 to 599,967
_RS03170
Position (kb) 596
597
598
599 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 595.960 kb on + strand, within MMJJ_RS03155 at 595.960 kb on + strand, within MMJJ_RS03155 at 595.986 kb on + strand, within MMJJ_RS03155 at 596.024 kb on - strand, within MMJJ_RS03155 at 596.029 kb on - strand, within MMJJ_RS03155 at 596.052 kb on + strand, within MMJJ_RS03155 at 596.060 kb on - strand, within MMJJ_RS03155 at 596.065 kb on - strand, within MMJJ_RS03155 at 596.065 kb on - strand, within MMJJ_RS03155 at 596.066 kb on + strand, within MMJJ_RS03155 at 596.067 kb on - strand, within MMJJ_RS03155 at 596.068 kb on + strand, within MMJJ_RS03155 at 596.081 kb on + strand, within MMJJ_RS03155 at 596.153 kb on + strand, within MMJJ_RS03155 at 596.155 kb on + strand, within MMJJ_RS03155 at 596.167 kb on - strand, within MMJJ_RS03155 at 596.192 kb on - strand, within MMJJ_RS03155 at 596.193 kb on + strand, within MMJJ_RS03155 at 596.247 kb on + strand, within MMJJ_RS03155 at 596.247 kb on + strand, within MMJJ_RS03155 at 596.299 kb on - strand, within MMJJ_RS03155 at 596.377 kb on + strand, within MMJJ_RS03155 at 596.384 kb on - strand, within MMJJ_RS03155 at 596.385 kb on + strand, within MMJJ_RS03155 at 596.388 kb on + strand, within MMJJ_RS03155 at 596.439 kb on + strand, within MMJJ_RS03155 at 596.439 kb on + strand, within MMJJ_RS03155 at 596.441 kb on + strand, within MMJJ_RS03155 at 596.536 kb on - strand, within MMJJ_RS03155 at 596.636 kb on + strand at 596.636 kb on + strand at 596.636 kb on + strand at 596.636 kb on + strand at 596.642 kb on + strand at 596.656 kb on - strand at 596.683 kb on + strand at 596.685 kb on + strand at 596.689 kb on - strand at 596.724 kb on - strand at 596.725 kb on + strand at 596.778 kb on - strand at 596.778 kb on - strand at 596.787 kb on - strand at 596.787 kb on - strand at 596.788 kb on + strand at 596.803 kb on - strand at 596.803 kb on - strand at 596.804 kb on + strand at 596.822 kb on - strand at 596.829 kb on - strand at 596.829 kb on - strand at 596.829 kb on - strand at 596.829 kb on - strand at 596.830 kb on + strand at 596.831 kb on - strand at 596.831 kb on - strand at 596.831 kb on - strand at 596.831 kb on - strand at 596.832 kb on + strand at 596.832 kb on + strand at 596.832 kb on + strand at 596.834 kb on - strand at 596.834 kb on - strand at 596.834 kb on - strand at 596.834 kb on - strand at 596.834 kb on - strand at 596.835 kb on + strand at 596.835 kb on + strand at 596.835 kb on + strand at 596.835 kb on + strand at 596.835 kb on + strand at 596.835 kb on + strand at 596.835 kb on + strand at 596.837 kb on + strand at 596.837 kb on + strand at 596.840 kb on + strand at 596.842 kb on - strand at 596.842 kb on - strand at 596.843 kb on + strand at 596.843 kb on + strand at 596.884 kb on - strand at 596.884 kb on - strand at 596.890 kb on - strand at 596.890 kb on - strand at 596.891 kb on + strand at 596.899 kb on - strand at 596.899 kb on - strand at 596.901 kb on - strand at 596.901 kb on - strand at 596.901 kb on - strand at 596.901 kb on - strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.902 kb on + strand at 596.906 kb on - strand at 596.909 kb on - strand at 596.910 kb on + strand at 596.914 kb on - strand at 596.915 kb on + strand at 596.915 kb on + strand at 596.922 kb on + strand at 596.944 kb on + strand at 596.950 kb on - strand at 596.950 kb on - strand at 596.973 kb on - strand at 596.974 kb on + strand at 596.974 kb on + strand at 596.974 kb on + strand at 597.000 kb on + strand at 597.077 kb on - strand at 597.087 kb on - strand at 597.088 kb on + strand at 597.141 kb on + strand, within MMJJ_RS03160 at 597.165 kb on - strand, within MMJJ_RS03160 at 597.166 kb on + strand, within MMJJ_RS03160 at 597.166 kb on + strand, within MMJJ_RS03160 at 597.167 kb on - strand, within MMJJ_RS03160 at 597.167 kb on - strand, within MMJJ_RS03160 at 597.168 kb on + strand, within MMJJ_RS03160 at 597.232 kb on - strand, within MMJJ_RS03160 at 597.277 kb on + strand, within MMJJ_RS03160 at 597.363 kb on + strand, within MMJJ_RS03160 at 597.383 kb on - strand, within MMJJ_RS03160 at 597.384 kb on + strand, within MMJJ_RS03160 at 597.393 kb on - strand, within MMJJ_RS03160 at 597.406 kb on + strand, within MMJJ_RS03160 at 597.420 kb on - strand, within MMJJ_RS03160 at 597.440 kb on - strand, within MMJJ_RS03160 at 597.451 kb on + strand, within MMJJ_RS03160 at 597.613 kb on + strand, within MMJJ_RS03160 at 597.614 kb on - strand, within MMJJ_RS03160 at 597.615 kb on + strand, within MMJJ_RS03160 at 597.646 kb on + strand, within MMJJ_RS03160 at 597.709 kb on + strand, within MMJJ_RS03160 at 597.717 kb on - strand, within MMJJ_RS03160 at 597.717 kb on - strand, within MMJJ_RS03160 at 597.717 kb on - strand, within MMJJ_RS03160 at 597.718 kb on + strand, within MMJJ_RS03160 at 597.718 kb on + strand, within MMJJ_RS03160 at 597.720 kb on - strand, within MMJJ_RS03160 at 597.721 kb on + strand, within MMJJ_RS03160 at 597.721 kb on + strand, within MMJJ_RS03160 at 597.724 kb on - strand, within MMJJ_RS03160 at 597.724 kb on - strand, within MMJJ_RS03160 at 597.725 kb on + strand, within MMJJ_RS03160 at 597.729 kb on - strand, within MMJJ_RS03160 at 597.730 kb on + strand, within MMJJ_RS03160 at 597.731 kb on - strand, within MMJJ_RS03160 at 597.731 kb on - strand, within MMJJ_RS03160 at 597.746 kb on + strand, within MMJJ_RS03160 at 597.762 kb on - strand, within MMJJ_RS03160 at 597.781 kb on + strand, within MMJJ_RS03160 at 597.781 kb on + strand, within MMJJ_RS03160 at 597.786 kb on - strand, within MMJJ_RS03160 at 597.789 kb on + strand, within MMJJ_RS03160 at 597.795 kb on + strand, within MMJJ_RS03160 at 597.813 kb on + strand, within MMJJ_RS03160 at 597.813 kb on + strand, within MMJJ_RS03160 at 597.814 kb on - strand, within MMJJ_RS03160 at 597.828 kb on - strand, within MMJJ_RS03160 at 597.828 kb on - strand, within MMJJ_RS03160 at 597.830 kb on - strand, within MMJJ_RS03160 at 597.918 kb on - strand, within MMJJ_RS03160 at 597.918 kb on - strand, within MMJJ_RS03160 at 597.924 kb on + strand, within MMJJ_RS03160 at 597.925 kb on - strand, within MMJJ_RS03160 at 597.925 kb on - strand, within MMJJ_RS03160 at 597.926 kb on + strand, within MMJJ_RS03160 at 597.926 kb on + strand, within MMJJ_RS03160 at 597.927 kb on - strand, within MMJJ_RS03160 at 597.927 kb on - strand, within MMJJ_RS03160 at 597.927 kb on - strand, within MMJJ_RS03160 at 597.928 kb on + strand, within MMJJ_RS03160 at 597.972 kb on + strand, within MMJJ_RS03160 at 597.979 kb on + strand, within MMJJ_RS03160 at 598.053 kb on + strand, within MMJJ_RS03160 at 598.071 kb on - strand, within MMJJ_RS03160 at 598.088 kb on - strand, within MMJJ_RS03160 at 598.089 kb on + strand, within MMJJ_RS03160 at 598.121 kb on - strand, within MMJJ_RS03160 at 598.121 kb on - strand, within MMJJ_RS03160 at 598.121 kb on - strand, within MMJJ_RS03160 at 598.122 kb on + strand, within MMJJ_RS03160 at 598.122 kb on + strand, within MMJJ_RS03160 at 598.124 kb on + strand, within MMJJ_RS03160 at 598.127 kb on - strand, within MMJJ_RS03160 at 598.128 kb on + strand, within MMJJ_RS03160 at 598.128 kb on + strand, within MMJJ_RS03160 at 598.204 kb on + strand at 598.210 kb on - strand at 598.212 kb on - strand at 598.213 kb on + strand at 598.213 kb on + strand at 598.213 kb on + strand at 598.255 kb on + strand at 598.281 kb on - strand at 598.281 kb on - strand at 598.285 kb on - strand at 598.286 kb on + strand at 598.286 kb on + strand at 598.293 kb on + strand at 598.295 kb on + strand at 598.299 kb on + strand at 598.322 kb on - strand at 598.322 kb on - strand at 598.322 kb on - strand at 598.323 kb on + strand at 598.323 kb on + strand at 598.327 kb on - strand at 598.383 kb on + strand at 598.458 kb on - strand, within MMJJ_RS03165 at 598.663 kb on + strand, within MMJJ_RS03165 at 599.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Spiramycin 0.1 mM remove 595,960 + MMJJ_RS03155 0.36 +0.8 595,960 + MMJJ_RS03155 0.36 -2.1 595,986 + MMJJ_RS03155 0.38 +0.2 596,024 - MMJJ_RS03155 0.42 +1.4 596,029 - MMJJ_RS03155 0.42 -0.3 596,052 + MMJJ_RS03155 0.45 -2.4 596,060 - MMJJ_RS03155 0.45 -3.0 596,065 - MMJJ_RS03155 0.46 -1.1 596,065 - MMJJ_RS03155 0.46 -0.7 596,066 + MMJJ_RS03155 0.46 -1.0 596,067 - MMJJ_RS03155 0.46 +0.0 596,068 + MMJJ_RS03155 0.46 -0.5 596,081 + MMJJ_RS03155 0.47 -1.8 596,153 + MMJJ_RS03155 0.54 -2.7 596,155 + MMJJ_RS03155 0.54 -1.2 596,167 - MMJJ_RS03155 0.55 -2.0 596,192 - MMJJ_RS03155 0.57 -0.2 596,193 + MMJJ_RS03155 0.58 +2.2 596,247 + MMJJ_RS03155 0.63 -0.5 596,247 + MMJJ_RS03155 0.63 -2.3 596,299 - MMJJ_RS03155 0.67 +0.6 596,377 + MMJJ_RS03155 0.74 -1.6 596,384 - MMJJ_RS03155 0.75 -2.2 596,385 + MMJJ_RS03155 0.75 -2.3 596,388 + MMJJ_RS03155 0.76 -1.2 596,439 + MMJJ_RS03155 0.80 -1.2 596,439 + MMJJ_RS03155 0.80 -0.0 596,441 + MMJJ_RS03155 0.80 +0.7 596,536 - MMJJ_RS03155 0.89 -0.7 596,636 + -0.7 596,636 + -0.1 596,636 + -1.6 596,636 + -0.3 596,642 + -2.4 596,656 - -2.7 596,683 + +0.2 596,685 + +0.8 596,689 - -0.5 596,724 - +0.4 596,725 + +2.4 596,778 - -3.3 596,778 - +1.1 596,787 - +1.6 596,787 - -1.2 596,788 + -0.3 596,803 - -0.9 596,803 - -0.2 596,804 + -1.3 596,822 - -4.1 596,829 - -1.6 596,829 - -0.9 596,829 - -0.5 596,829 - -1.7 596,830 + +0.1 596,831 - +0.2 596,831 - +1.5 596,831 - +0.7 596,831 - -2.2 596,832 + -1.9 596,832 + +1.1 596,832 + -0.5 596,834 - +1.6 596,834 - -0.9 596,834 - -2.1 596,834 - -2.5 596,834 - -1.0 596,835 + -1.0 596,835 + -0.7 596,835 + -2.0 596,835 + -2.0 596,835 + -2.1 596,835 + -2.1 596,835 + +0.0 596,837 + +2.0 596,837 + -2.2 596,840 + +2.0 596,842 - +1.0 596,842 - +0.1 596,843 + -0.2 596,843 + -0.2 596,884 - -0.9 596,884 - +2.0 596,890 - +1.7 596,890 - +1.9 596,891 + +1.3 596,899 - +0.8 596,899 - +1.1 596,901 - -1.5 596,901 - +4.7 596,901 - +2.8 596,901 - -0.9 596,902 + -1.7 596,902 + -0.5 596,902 + +1.0 596,902 + +1.1 596,902 + +1.6 596,902 + +0.4 596,902 + -2.0 596,902 + +0.3 596,906 - +3.9 596,909 - +0.1 596,910 + -1.2 596,914 - -0.1 596,915 + +1.8 596,915 + +0.1 596,922 + +0.5 596,944 + +2.2 596,950 - -1.2 596,950 - -0.6 596,973 - -2.4 596,974 + -0.2 596,974 + -0.7 596,974 + +1.1 597,000 + -1.8 597,077 - -4.1 597,087 - -2.4 597,088 + -0.9 597,141 + MMJJ_RS03160 0.13 -0.3 597,165 - MMJJ_RS03160 0.15 -2.5 597,166 + MMJJ_RS03160 0.15 -3.0 597,166 + MMJJ_RS03160 0.15 -2.3 597,167 - MMJJ_RS03160 0.15 -3.7 597,167 - MMJJ_RS03160 0.15 -4.0 597,168 + MMJJ_RS03160 0.15 -3.6 597,232 - MMJJ_RS03160 0.20 -1.5 597,277 + MMJJ_RS03160 0.23 -3.5 597,363 + MMJJ_RS03160 0.30 -1.7 597,383 - MMJJ_RS03160 0.31 -1.5 597,384 + MMJJ_RS03160 0.31 -2.0 597,393 - MMJJ_RS03160 0.32 -1.8 597,406 + MMJJ_RS03160 0.33 -0.9 597,420 - MMJJ_RS03160 0.34 -4.1 597,440 - MMJJ_RS03160 0.35 -3.3 597,451 + MMJJ_RS03160 0.36 -2.3 597,613 + MMJJ_RS03160 0.48 -0.6 597,614 - MMJJ_RS03160 0.48 -1.8 597,615 + MMJJ_RS03160 0.48 -0.9 597,646 + MMJJ_RS03160 0.51 -1.3 597,709 + MMJJ_RS03160 0.55 -1.0 597,717 - MMJJ_RS03160 0.56 -3.1 597,717 - MMJJ_RS03160 0.56 -1.3 597,717 - MMJJ_RS03160 0.56 -0.9 597,718 + MMJJ_RS03160 0.56 -3.3 597,718 + MMJJ_RS03160 0.56 -2.0 597,720 - MMJJ_RS03160 0.56 -1.9 597,721 + MMJJ_RS03160 0.56 -2.0 597,721 + MMJJ_RS03160 0.56 -2.0 597,724 - MMJJ_RS03160 0.56 -3.6 597,724 - MMJJ_RS03160 0.56 -3.5 597,725 + MMJJ_RS03160 0.56 -1.2 597,729 - MMJJ_RS03160 0.57 -2.2 597,730 + MMJJ_RS03160 0.57 -2.7 597,731 - MMJJ_RS03160 0.57 -1.2 597,731 - MMJJ_RS03160 0.57 -0.5 597,746 + MMJJ_RS03160 0.58 -4.3 597,762 - MMJJ_RS03160 0.59 -0.1 597,781 + MMJJ_RS03160 0.60 -3.7 597,781 + MMJJ_RS03160 0.60 -3.0 597,786 - MMJJ_RS03160 0.61 -1.0 597,789 + MMJJ_RS03160 0.61 -1.4 597,795 + MMJJ_RS03160 0.61 -0.5 597,813 + MMJJ_RS03160 0.63 -3.3 597,813 + MMJJ_RS03160 0.63 -3.1 597,814 - MMJJ_RS03160 0.63 -2.9 597,828 - MMJJ_RS03160 0.64 -2.3 597,828 - MMJJ_RS03160 0.64 -5.0 597,830 - MMJJ_RS03160 0.64 -0.6 597,918 - MMJJ_RS03160 0.70 -2.5 597,918 - MMJJ_RS03160 0.70 -3.3 597,924 + MMJJ_RS03160 0.71 +0.3 597,925 - MMJJ_RS03160 0.71 -3.2 597,925 - MMJJ_RS03160 0.71 -2.9 597,926 + MMJJ_RS03160 0.71 -1.3 597,926 + MMJJ_RS03160 0.71 -1.7 597,927 - MMJJ_RS03160 0.71 -1.2 597,927 - MMJJ_RS03160 0.71 -2.3 597,927 - MMJJ_RS03160 0.71 -4.5 597,928 + MMJJ_RS03160 0.71 -2.7 597,972 + MMJJ_RS03160 0.74 -0.6 597,979 + MMJJ_RS03160 0.75 -2.6 598,053 + MMJJ_RS03160 0.80 -1.7 598,071 - MMJJ_RS03160 0.82 +0.7 598,088 - MMJJ_RS03160 0.83 -2.4 598,089 + MMJJ_RS03160 0.83 -1.9 598,121 - MMJJ_RS03160 0.85 -2.6 598,121 - MMJJ_RS03160 0.85 -1.1 598,121 - MMJJ_RS03160 0.85 -2.2 598,122 + MMJJ_RS03160 0.85 -0.4 598,122 + MMJJ_RS03160 0.85 -0.9 598,124 + MMJJ_RS03160 0.86 -1.6 598,127 - MMJJ_RS03160 0.86 -4.9 598,128 + MMJJ_RS03160 0.86 -2.3 598,128 + MMJJ_RS03160 0.86 -2.7 598,204 + -0.9 598,210 - -3.3 598,212 - -0.5 598,213 + -1.0 598,213 + -2.2 598,213 + -2.6 598,255 + -4.3 598,281 - -4.0 598,281 - -1.3 598,285 - -3.0 598,286 + -4.6 598,286 + -1.3 598,293 + -1.4 598,295 + -2.2 598,299 + -2.5 598,322 - -2.5 598,322 - -0.5 598,322 - -0.8 598,323 + +2.1 598,323 + -2.8 598,327 - -0.3 598,383 + +1.5 598,458 - MMJJ_RS03165 0.13 -1.5 598,663 + MMJJ_RS03165 0.35 -1.3 599,309 - -3.6
Or see this region's nucleotide sequence