Experiment: McCas_formate_complete Spiramycin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01215 and MMJJ_RS01220 are separated by 98 nucleotides MMJJ_RS01220 and MMJJ_RS01225 are separated by 21 nucleotides MMJJ_RS01225 and MMJJ_RS01230 are separated by 9 nucleotides MMJJ_RS01230 and MMJJ_RS01235 are separated by 126 nucleotides
MMJJ_RS01215: MMJJ_RS01215 - MATE family efflux transporter, at 232,784 to 234,142
_RS01215
MMJJ_RS01220: MMJJ_RS01220 - exodeoxyribonuclease VII large subunit, at 234,241 to 235,464
_RS01220
MMJJ_RS01225: MMJJ_RS01225 - exodeoxyribonuclease VII small subunit, at 235,486 to 235,689
_RS01225
MMJJ_RS01230: MMJJ_RS01230 - EamA family transporter, at 235,699 to 236,118
_RS01230
MMJJ_RS01235: MMJJ_RS01235 - excinuclease ABC subunit UvrA, at 236,245 to 239,100
_RS01235
Position (kb) 234
235
236 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 233.254 kb on - strand, within MMJJ_RS01215 at 233.254 kb on - strand, within MMJJ_RS01215 at 233.254 kb on - strand, within MMJJ_RS01215 at 233.312 kb on + strand, within MMJJ_RS01215 at 233.318 kb on + strand, within MMJJ_RS01215 at 233.320 kb on + strand, within MMJJ_RS01215 at 233.327 kb on - strand, within MMJJ_RS01215 at 233.327 kb on - strand, within MMJJ_RS01215 at 233.329 kb on - strand, within MMJJ_RS01215 at 233.329 kb on - strand, within MMJJ_RS01215 at 233.330 kb on + strand, within MMJJ_RS01215 at 233.331 kb on - strand, within MMJJ_RS01215 at 233.331 kb on - strand, within MMJJ_RS01215 at 233.331 kb on - strand, within MMJJ_RS01215 at 233.332 kb on + strand, within MMJJ_RS01215 at 233.332 kb on + strand, within MMJJ_RS01215 at 233.337 kb on - strand, within MMJJ_RS01215 at 233.341 kb on - strand, within MMJJ_RS01215 at 233.342 kb on + strand, within MMJJ_RS01215 at 233.361 kb on - strand, within MMJJ_RS01215 at 233.416 kb on + strand, within MMJJ_RS01215 at 233.522 kb on + strand, within MMJJ_RS01215 at 233.554 kb on + strand, within MMJJ_RS01215 at 233.556 kb on - strand, within MMJJ_RS01215 at 233.616 kb on - strand, within MMJJ_RS01215 at 233.617 kb on + strand, within MMJJ_RS01215 at 233.619 kb on - strand, within MMJJ_RS01215 at 233.619 kb on - strand, within MMJJ_RS01215 at 233.621 kb on - strand, within MMJJ_RS01215 at 233.621 kb on - strand, within MMJJ_RS01215 at 233.621 kb on - strand, within MMJJ_RS01215 at 233.621 kb on - strand, within MMJJ_RS01215 at 233.622 kb on + strand, within MMJJ_RS01215 at 233.623 kb on - strand, within MMJJ_RS01215 at 233.623 kb on - strand, within MMJJ_RS01215 at 233.624 kb on + strand, within MMJJ_RS01215 at 233.648 kb on - strand, within MMJJ_RS01215 at 233.687 kb on - strand, within MMJJ_RS01215 at 233.727 kb on - strand, within MMJJ_RS01215 at 233.728 kb on + strand, within MMJJ_RS01215 at 233.887 kb on + strand, within MMJJ_RS01215 at 233.902 kb on - strand, within MMJJ_RS01215 at 233.903 kb on + strand, within MMJJ_RS01215 at 233.919 kb on + strand, within MMJJ_RS01215 at 233.919 kb on + strand, within MMJJ_RS01215 at 233.926 kb on + strand, within MMJJ_RS01215 at 233.984 kb on + strand, within MMJJ_RS01215 at 233.998 kb on - strand, within MMJJ_RS01215 at 233.999 kb on + strand, within MMJJ_RS01215 at 234.003 kb on - strand, within MMJJ_RS01215 at 234.013 kb on + strand at 234.013 kb on + strand at 234.014 kb on - strand at 234.014 kb on - strand at 234.014 kb on - strand at 234.014 kb on - strand at 234.014 kb on - strand at 234.014 kb on - strand at 234.014 kb on - strand at 234.015 kb on + strand at 234.015 kb on + strand at 234.026 kb on + strand at 234.033 kb on - strand at 234.033 kb on - strand at 234.059 kb on - strand at 234.060 kb on + strand at 234.060 kb on + strand at 234.071 kb on + strand at 234.074 kb on + strand at 234.083 kb on - strand at 234.083 kb on - strand at 234.091 kb on + strand at 234.091 kb on + strand at 234.104 kb on + strand at 234.127 kb on + strand at 234.127 kb on + strand at 234.152 kb on - strand at 234.153 kb on + strand at 234.153 kb on + strand at 234.172 kb on + strand at 234.175 kb on - strand at 234.176 kb on + strand at 234.176 kb on + strand at 234.178 kb on - strand at 234.179 kb on + strand at 234.180 kb on - strand at 234.181 kb on + strand at 234.190 kb on - strand at 234.193 kb on - strand at 234.206 kb on - strand at 234.206 kb on - strand at 234.207 kb on + strand at 234.241 kb on + strand at 234.308 kb on - strand at 234.352 kb on + strand at 234.376 kb on + strand, within MMJJ_RS01220 at 234.380 kb on - strand, within MMJJ_RS01220 at 234.381 kb on + strand, within MMJJ_RS01220 at 234.385 kb on + strand, within MMJJ_RS01220 at 234.454 kb on - strand, within MMJJ_RS01220 at 234.457 kb on + strand, within MMJJ_RS01220 at 234.489 kb on - strand, within MMJJ_RS01220 at 234.497 kb on - strand, within MMJJ_RS01220 at 234.497 kb on - strand, within MMJJ_RS01220 at 234.500 kb on - strand, within MMJJ_RS01220 at 234.500 kb on - strand, within MMJJ_RS01220 at 234.550 kb on - strand, within MMJJ_RS01220 at 234.551 kb on + strand, within MMJJ_RS01220 at 234.551 kb on + strand, within MMJJ_RS01220 at 234.555 kb on - strand, within MMJJ_RS01220 at 234.587 kb on - strand, within MMJJ_RS01220 at 234.693 kb on - strand, within MMJJ_RS01220 at 234.693 kb on - strand, within MMJJ_RS01220 at 234.723 kb on - strand, within MMJJ_RS01220 at 234.764 kb on - strand, within MMJJ_RS01220 at 234.765 kb on + strand, within MMJJ_RS01220 at 234.768 kb on - strand, within MMJJ_RS01220 at 234.769 kb on + strand, within MMJJ_RS01220 at 234.771 kb on + strand, within MMJJ_RS01220 at 234.923 kb on - strand, within MMJJ_RS01220 at 234.924 kb on + strand, within MMJJ_RS01220 at 235.049 kb on - strand, within MMJJ_RS01220 at 235.050 kb on + strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.051 kb on - strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.052 kb on + strand, within MMJJ_RS01220 at 235.053 kb on - strand, within MMJJ_RS01220 at 235.053 kb on - strand, within MMJJ_RS01220 at 235.054 kb on + strand, within MMJJ_RS01220 at 235.084 kb on + strand, within MMJJ_RS01220 at 235.101 kb on + strand, within MMJJ_RS01220 at 235.113 kb on + strand, within MMJJ_RS01220 at 235.138 kb on - strand, within MMJJ_RS01220 at 235.145 kb on - strand, within MMJJ_RS01220 at 235.188 kb on - strand, within MMJJ_RS01220 at 235.310 kb on + strand, within MMJJ_RS01220 at 235.310 kb on + strand, within MMJJ_RS01220 at 235.311 kb on - strand, within MMJJ_RS01220 at 235.332 kb on - strand, within MMJJ_RS01220 at 235.340 kb on + strand, within MMJJ_RS01220 at 235.350 kb on + strand at 235.373 kb on - strand at 235.373 kb on - strand at 235.447 kb on + strand at 235.678 kb on + strand at 235.702 kb on - strand at 235.737 kb on - strand at 235.737 kb on - strand at 235.738 kb on + strand at 235.738 kb on + strand at 235.787 kb on + strand, within MMJJ_RS01230 at 235.803 kb on - strand, within MMJJ_RS01230 at 235.803 kb on - strand, within MMJJ_RS01230 at 235.804 kb on + strand, within MMJJ_RS01230 at 235.842 kb on - strand, within MMJJ_RS01230 at 235.842 kb on - strand, within MMJJ_RS01230 at 235.847 kb on - strand, within MMJJ_RS01230 at 235.871 kb on - strand, within MMJJ_RS01230 at 235.872 kb on + strand, within MMJJ_RS01230 at 235.872 kb on + strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.903 kb on - strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.904 kb on + strand, within MMJJ_RS01230 at 235.947 kb on + strand, within MMJJ_RS01230 at 235.997 kb on + strand, within MMJJ_RS01230 at 236.027 kb on - strand, within MMJJ_RS01230 at 236.113 kb on + strand at 236.143 kb on - strand at 236.144 kb on + strand at 236.144 kb on + strand at 236.145 kb on - strand at 236.145 kb on - strand at 236.145 kb on - strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.146 kb on + strand at 236.147 kb on - strand at 236.188 kb on + strand at 236.199 kb on - strand at 236.199 kb on - strand at 236.199 kb on - strand at 236.199 kb on - strand at 236.202 kb on + strand at 236.240 kb on - strand at 236.241 kb on + strand at 236.245 kb on + strand at 236.253 kb on - strand at 236.254 kb on + strand at 236.261 kb on - strand at 236.280 kb on - strand at 236.281 kb on + strand at 236.286 kb on + strand at 236.325 kb on + strand at 236.330 kb on - strand at 236.364 kb on + strand at 236.373 kb on - strand at 236.374 kb on + strand at 236.379 kb on + strand at 236.380 kb on - strand at 236.381 kb on + strand at 236.401 kb on - strand at 236.402 kb on + strand at 236.402 kb on + strand at 236.423 kb on + strand at 236.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Spiramycin 0.1 mM remove 233,254 - MMJJ_RS01215 0.35 +1.2 233,254 - MMJJ_RS01215 0.35 -0.4 233,254 - MMJJ_RS01215 0.35 +0.0 233,312 + MMJJ_RS01215 0.39 -2.2 233,318 + MMJJ_RS01215 0.39 -1.7 233,320 + MMJJ_RS01215 0.39 -0.9 233,327 - MMJJ_RS01215 0.40 +0.9 233,327 - MMJJ_RS01215 0.40 +1.7 233,329 - MMJJ_RS01215 0.40 +0.6 233,329 - MMJJ_RS01215 0.40 -0.0 233,330 + MMJJ_RS01215 0.40 +1.9 233,331 - MMJJ_RS01215 0.40 +1.3 233,331 - MMJJ_RS01215 0.40 +2.0 233,331 - MMJJ_RS01215 0.40 -0.9 233,332 + MMJJ_RS01215 0.40 +1.7 233,332 + MMJJ_RS01215 0.40 -1.8 233,337 - MMJJ_RS01215 0.41 -1.9 233,341 - MMJJ_RS01215 0.41 +1.4 233,342 + MMJJ_RS01215 0.41 -1.0 233,361 - MMJJ_RS01215 0.42 -2.6 233,416 + MMJJ_RS01215 0.47 -0.5 233,522 + MMJJ_RS01215 0.54 -4.9 233,554 + MMJJ_RS01215 0.57 +1.8 233,556 - MMJJ_RS01215 0.57 -1.8 233,616 - MMJJ_RS01215 0.61 -1.6 233,617 + MMJJ_RS01215 0.61 +0.1 233,619 - MMJJ_RS01215 0.61 -0.2 233,619 - MMJJ_RS01215 0.61 -0.7 233,621 - MMJJ_RS01215 0.62 +1.0 233,621 - MMJJ_RS01215 0.62 +0.5 233,621 - MMJJ_RS01215 0.62 +0.6 233,621 - MMJJ_RS01215 0.62 +3.3 233,622 + MMJJ_RS01215 0.62 -0.6 233,623 - MMJJ_RS01215 0.62 -0.9 233,623 - MMJJ_RS01215 0.62 -1.1 233,624 + MMJJ_RS01215 0.62 +0.9 233,648 - MMJJ_RS01215 0.64 +2.3 233,687 - MMJJ_RS01215 0.66 +0.2 233,727 - MMJJ_RS01215 0.69 +1.8 233,728 + MMJJ_RS01215 0.69 +1.4 233,887 + MMJJ_RS01215 0.81 +0.0 233,902 - MMJJ_RS01215 0.82 +1.4 233,903 + MMJJ_RS01215 0.82 -2.1 233,919 + MMJJ_RS01215 0.84 -0.9 233,919 + MMJJ_RS01215 0.84 -0.6 233,926 + MMJJ_RS01215 0.84 +0.4 233,984 + MMJJ_RS01215 0.88 +3.4 233,998 - MMJJ_RS01215 0.89 +0.1 233,999 + MMJJ_RS01215 0.89 -1.0 234,003 - MMJJ_RS01215 0.90 -0.2 234,013 + +1.2 234,013 + +0.3 234,014 - +0.2 234,014 - -1.5 234,014 - -1.1 234,014 - +1.8 234,014 - -3.3 234,014 - +2.1 234,014 - -2.4 234,015 + -2.3 234,015 + +2.2 234,026 + +1.0 234,033 - -0.9 234,033 - -1.7 234,059 - +0.9 234,060 + +1.7 234,060 + +2.0 234,071 + -4.9 234,074 + -0.4 234,083 - -0.3 234,083 - +0.5 234,091 + +0.2 234,091 + -2.7 234,104 + +0.4 234,127 + +3.5 234,127 + +0.2 234,152 - +0.7 234,153 + -2.0 234,153 + -0.5 234,172 + +2.1 234,175 - -0.0 234,176 + +0.4 234,176 + +1.2 234,178 - -1.6 234,179 + -1.7 234,180 - -0.7 234,181 + -1.7 234,190 - +0.6 234,193 - +1.4 234,206 - +0.5 234,206 - +0.2 234,207 + -0.8 234,241 + -2.2 234,308 - -1.5 234,352 + +0.4 234,376 + MMJJ_RS01220 0.11 -2.3 234,380 - MMJJ_RS01220 0.11 +0.7 234,381 + MMJJ_RS01220 0.11 +0.2 234,385 + MMJJ_RS01220 0.12 +0.4 234,454 - MMJJ_RS01220 0.17 -1.6 234,457 + MMJJ_RS01220 0.18 -0.8 234,489 - MMJJ_RS01220 0.20 -0.4 234,497 - MMJJ_RS01220 0.21 -0.4 234,497 - MMJJ_RS01220 0.21 -1.3 234,500 - MMJJ_RS01220 0.21 +0.2 234,500 - MMJJ_RS01220 0.21 -2.8 234,550 - MMJJ_RS01220 0.25 -1.0 234,551 + MMJJ_RS01220 0.25 +0.1 234,551 + MMJJ_RS01220 0.25 +1.5 234,555 - MMJJ_RS01220 0.26 +1.3 234,587 - MMJJ_RS01220 0.28 +1.9 234,693 - MMJJ_RS01220 0.37 -2.8 234,693 - MMJJ_RS01220 0.37 -0.8 234,723 - MMJJ_RS01220 0.39 -1.3 234,764 - MMJJ_RS01220 0.43 +0.9 234,765 + MMJJ_RS01220 0.43 -0.9 234,768 - MMJJ_RS01220 0.43 +2.9 234,769 + MMJJ_RS01220 0.43 +2.4 234,771 + MMJJ_RS01220 0.43 -1.3 234,923 - MMJJ_RS01220 0.56 +1.3 234,924 + MMJJ_RS01220 0.56 +0.1 235,049 - MMJJ_RS01220 0.66 -0.2 235,050 + MMJJ_RS01220 0.66 -1.5 235,051 - MMJJ_RS01220 0.66 +0.3 235,051 - MMJJ_RS01220 0.66 -2.5 235,051 - MMJJ_RS01220 0.66 -0.6 235,051 - MMJJ_RS01220 0.66 -1.2 235,051 - MMJJ_RS01220 0.66 -2.4 235,051 - MMJJ_RS01220 0.66 +1.3 235,051 - MMJJ_RS01220 0.66 -3.3 235,051 - MMJJ_RS01220 0.66 +0.8 235,051 - MMJJ_RS01220 0.66 -1.7 235,052 + MMJJ_RS01220 0.66 -1.1 235,052 + MMJJ_RS01220 0.66 -0.1 235,052 + MMJJ_RS01220 0.66 -0.1 235,052 + MMJJ_RS01220 0.66 +0.9 235,052 + MMJJ_RS01220 0.66 +0.1 235,052 + MMJJ_RS01220 0.66 -1.2 235,052 + MMJJ_RS01220 0.66 -1.8 235,052 + MMJJ_RS01220 0.66 -0.0 235,052 + MMJJ_RS01220 0.66 -1.9 235,052 + MMJJ_RS01220 0.66 +1.1 235,052 + MMJJ_RS01220 0.66 +0.2 235,053 - MMJJ_RS01220 0.66 +1.9 235,053 - MMJJ_RS01220 0.66 -0.5 235,054 + MMJJ_RS01220 0.66 -2.0 235,084 + MMJJ_RS01220 0.69 +0.5 235,101 + MMJJ_RS01220 0.70 -1.5 235,113 + MMJJ_RS01220 0.71 -1.1 235,138 - MMJJ_RS01220 0.73 -0.9 235,145 - MMJJ_RS01220 0.74 +2.2 235,188 - MMJJ_RS01220 0.77 -1.7 235,310 + MMJJ_RS01220 0.87 +0.1 235,310 + MMJJ_RS01220 0.87 -1.0 235,311 - MMJJ_RS01220 0.87 -1.4 235,332 - MMJJ_RS01220 0.89 +1.8 235,340 + MMJJ_RS01220 0.90 -1.5 235,350 + -1.6 235,373 - -0.7 235,373 - -0.4 235,447 + -0.4 235,678 + +0.3 235,702 - -0.0 235,737 - -0.1 235,737 - -1.6 235,738 + -1.0 235,738 + -0.6 235,787 + MMJJ_RS01230 0.21 +0.3 235,803 - MMJJ_RS01230 0.25 +1.4 235,803 - MMJJ_RS01230 0.25 -1.2 235,804 + MMJJ_RS01230 0.25 -1.2 235,842 - MMJJ_RS01230 0.34 +1.4 235,842 - MMJJ_RS01230 0.34 -0.2 235,847 - MMJJ_RS01230 0.35 -1.2 235,871 - MMJJ_RS01230 0.41 +1.0 235,872 + MMJJ_RS01230 0.41 +0.3 235,872 + MMJJ_RS01230 0.41 -2.8 235,903 - MMJJ_RS01230 0.49 -0.9 235,903 - MMJJ_RS01230 0.49 -0.7 235,903 - MMJJ_RS01230 0.49 +2.3 235,903 - MMJJ_RS01230 0.49 +1.9 235,904 + MMJJ_RS01230 0.49 -0.7 235,904 + MMJJ_RS01230 0.49 +0.2 235,904 + MMJJ_RS01230 0.49 +0.7 235,904 + MMJJ_RS01230 0.49 -0.4 235,904 + MMJJ_RS01230 0.49 +0.8 235,904 + MMJJ_RS01230 0.49 -1.0 235,947 + MMJJ_RS01230 0.59 -4.2 235,997 + MMJJ_RS01230 0.71 -0.1 236,027 - MMJJ_RS01230 0.78 -2.7 236,113 + -0.2 236,143 - -0.7 236,144 + +0.4 236,144 + +0.7 236,145 - +1.3 236,145 - +3.1 236,145 - -0.7 236,146 + -3.1 236,146 + +0.6 236,146 + -0.4 236,146 + +2.2 236,146 + +0.3 236,146 + -0.3 236,146 + +0.7 236,146 + +0.6 236,146 + +1.2 236,146 + -3.6 236,147 - +0.7 236,188 + -0.5 236,199 - +2.0 236,199 - -3.9 236,199 - -0.1 236,199 - -2.2 236,202 + -1.2 236,240 - -2.6 236,241 + +0.3 236,245 + +0.6 236,253 - +1.1 236,254 + -0.6 236,261 - +0.5 236,280 - +0.0 236,281 + +0.7 236,286 + +0.4 236,325 + +0.3 236,330 - +0.4 236,364 + -0.2 236,373 - -3.5 236,374 + +1.7 236,379 + +1.2 236,380 - -1.4 236,381 + +0.8 236,401 - -0.4 236,402 + -0.1 236,402 + +0.4 236,423 + -1.3 236,452 + +1.5
Or see this region's nucleotide sequence