Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS04965

Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS04955 and MMJJ_RS04960 are separated by 85 nucleotidesMMJJ_RS04960 and MMJJ_RS04965 are separated by 48 nucleotidesMMJJ_RS04965 and MMJJ_RS04970 are separated by 100 nucleotidesMMJJ_RS04970 and MMJJ_RS04975 are separated by 22 nucleotides MMJJ_RS04955: MMJJ_RS04955 - tRNA pseudouridine(54/55) synthase Pus10, at 920,964 to 922,376 _RS04955 MMJJ_RS04960: MMJJ_RS04960 - 50S ribosomal protein L21e, at 922,462 to 922,755 _RS04960 MMJJ_RS04965: MMJJ_RS04965 - RNA polymerase Rpb4 family protein, at 922,804 to 923,127 _RS04965 MMJJ_RS04970: MMJJ_RS04970 - DUF655 domain-containing protein, at 923,228 to 923,812 _RS04970 MMJJ_RS04975: MMJJ_RS04975 - glycosyltransferase family 4 protein, at 923,835 to 924,902 _RS04975 Position (kb) 922 923 924Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 921.874 kb on - strand, within MMJJ_RS04955at 921.958 kb on - strand, within MMJJ_RS04955at 922.026 kb on - strand, within MMJJ_RS04955at 922.026 kb on - strand, within MMJJ_RS04955at 922.027 kb on + strand, within MMJJ_RS04955at 922.171 kb on - strand, within MMJJ_RS04955at 922.172 kb on + strand, within MMJJ_RS04955at 922.242 kb on - strandat 922.243 kb on + strandat 922.256 kb on - strandat 922.303 kb on - strandat 922.307 kb on - strandat 922.316 kb on - strandat 922.316 kb on - strandat 922.317 kb on + strandat 922.317 kb on + strandat 922.322 kb on - strandat 922.330 kb on - strandat 922.353 kb on - strandat 922.375 kb on + strandat 922.747 kb on + strandat 922.757 kb on - strandat 922.762 kb on - strandat 922.803 kb on - strandat 922.821 kb on - strandat 922.943 kb on - strand, within MMJJ_RS04965at 922.943 kb on - strand, within MMJJ_RS04965at 922.943 kb on - strand, within MMJJ_RS04965at 922.947 kb on - strand, within MMJJ_RS04965at 922.947 kb on - strand, within MMJJ_RS04965at 922.948 kb on + strand, within MMJJ_RS04965at 922.966 kb on + strand, within MMJJ_RS04965at 923.061 kb on + strand, within MMJJ_RS04965at 923.065 kb on - strand, within MMJJ_RS04965at 923.065 kb on - strand, within MMJJ_RS04965at 923.068 kb on + strand, within MMJJ_RS04965at 923.108 kb on - strandat 923.108 kb on - strandat 923.109 kb on + strandat 923.116 kb on + strandat 923.144 kb on - strandat 923.145 kb on + strandat 923.145 kb on + strandat 923.147 kb on - strandat 923.187 kb on + strandat 923.190 kb on - strandat 923.190 kb on - strandat 923.191 kb on + strandat 923.196 kb on - strandat 923.318 kb on + strand, within MMJJ_RS04970at 923.429 kb on - strand, within MMJJ_RS04970at 923.613 kb on - strand, within MMJJ_RS04970at 923.613 kb on - strand, within MMJJ_RS04970at 923.860 kb on + strandat 924.025 kb on - strand, within MMJJ_RS04975at 924.112 kb on + strand, within MMJJ_RS04975

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
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921,874 - MMJJ_RS04955 0.64 +3.3
921,958 - MMJJ_RS04955 0.70 -1.7
922,026 - MMJJ_RS04955 0.75 +0.2
922,026 - MMJJ_RS04955 0.75 -2.5
922,027 + MMJJ_RS04955 0.75 -0.8
922,171 - MMJJ_RS04955 0.85 +2.5
922,172 + MMJJ_RS04955 0.85 -2.5
922,242 - +5.7
922,243 + -2.9
922,256 - -3.7
922,303 - +2.2
922,307 - +1.9
922,316 - +0.4
922,316 - +1.7
922,317 + -1.2
922,317 + -1.3
922,322 - +4.6
922,330 - +1.3
922,353 - +3.3
922,375 + +0.0
922,747 + -1.4
922,757 - -2.0
922,762 - +0.8
922,803 - -1.4
922,821 - -0.6
922,943 - MMJJ_RS04965 0.43 -1.4
922,943 - MMJJ_RS04965 0.43 -1.9
922,943 - MMJJ_RS04965 0.43 -1.5
922,947 - MMJJ_RS04965 0.44 -1.5
922,947 - MMJJ_RS04965 0.44 -0.2
922,948 + MMJJ_RS04965 0.44 -0.3
922,966 + MMJJ_RS04965 0.50 +0.5
923,061 + MMJJ_RS04965 0.79 +0.5
923,065 - MMJJ_RS04965 0.81 -0.6
923,065 - MMJJ_RS04965 0.81 +1.2
923,068 + MMJJ_RS04965 0.81 -1.0
923,108 - +1.6
923,108 - -2.2
923,109 + +1.2
923,116 + -2.5
923,144 - +2.5
923,145 + -1.6
923,145 + -3.3
923,147 - +3.2
923,187 + +0.0
923,190 - -1.9
923,190 - +0.5
923,191 + -1.2
923,196 - +1.2
923,318 + MMJJ_RS04970 0.15 -1.8
923,429 - MMJJ_RS04970 0.34 -1.3
923,613 - MMJJ_RS04970 0.66 -2.2
923,613 - MMJJ_RS04970 0.66 -0.6
923,860 + +0.5
924,025 - MMJJ_RS04975 0.18 -1.0
924,112 + MMJJ_RS04975 0.26 -1.3

Or see this region's nucleotide sequence