Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS02345

Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS02340 and MMJJ_RS02345 are separated by 349 nucleotidesMMJJ_RS02345 and MMJJ_RS02350 are separated by 208 nucleotides MMJJ_RS02340: MMJJ_RS02340 - SAM-dependent methyltransferase, at 442,590 to 443,138 _RS02340 MMJJ_RS02345: MMJJ_RS02345 - ATP-binding protein, at 443,488 to 444,759 _RS02345 MMJJ_RS02350: MMJJ_RS02350 - Fic family protein, at 444,968 to 446,257 _RS02350 Position (kb) 443 444 445Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5 6at 442.532 kb on + strandat 442.536 kb on + strandat 442.536 kb on + strandat 442.546 kb on + strandat 442.592 kb on + strandat 442.614 kb on - strandat 442.619 kb on - strandat 442.620 kb on + strandat 442.670 kb on + strand, within MMJJ_RS02340at 442.670 kb on + strand, within MMJJ_RS02340at 442.740 kb on - strand, within MMJJ_RS02340at 442.740 kb on - strand, within MMJJ_RS02340at 442.740 kb on - strand, within MMJJ_RS02340at 442.740 kb on - strandat 442.740 kb on - strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.741 kb on + strand, within MMJJ_RS02340at 442.765 kb on + strand, within MMJJ_RS02340at 442.765 kb on + strand, within MMJJ_RS02340at 442.906 kb on - strand, within MMJJ_RS02340at 442.906 kb on - strand, within MMJJ_RS02340at 442.906 kb on - strand, within MMJJ_RS02340at 442.907 kb on + strand, within MMJJ_RS02340at 443.078 kb on - strand, within MMJJ_RS02340at 443.079 kb on + strand, within MMJJ_RS02340at 443.082 kb on + strand, within MMJJ_RS02340at 443.082 kb on + strand, within MMJJ_RS02340at 443.214 kb on + strandat 443.218 kb on - strandat 443.236 kb on - strandat 443.236 kb on - strandat 443.237 kb on + strandat 443.238 kb on - strandat 443.238 kb on - strandat 443.239 kb on + strandat 443.240 kb on - strandat 443.240 kb on - strandat 443.288 kb on + strandat 443.301 kb on + strandat 443.337 kb on - strandat 443.350 kb on - strandat 443.354 kb on - strandat 443.355 kb on + strandat 443.384 kb on - strandat 443.384 kb on - strandat 443.394 kb on + strandat 443.398 kb on - strandat 443.402 kb on - strandat 443.463 kb on - strandat 443.463 kb on - strandat 443.463 kb on - strandat 443.463 kb on - strandat 443.463 kb on - strandat 443.464 kb on + strandat 443.464 kb on + strandat 443.464 kb on + strandat 443.464 kb on + strandat 443.464 kb on + strandat 443.464 kb on + strandat 443.465 kb on - strandat 443.465 kb on - strandat 443.465 kb on - strandat 443.465 kb on - strandat 443.466 kb on + strandat 443.466 kb on + strandat 443.467 kb on - strandat 443.467 kb on - strandat 443.468 kb on + strandat 443.468 kb on + strandat 443.468 kb on + strandat 443.472 kb on + strandat 443.490 kb on + strandat 443.494 kb on + strandat 443.537 kb on + strandat 443.538 kb on - strandat 443.539 kb on + strandat 443.564 kb on + strandat 443.583 kb on + strandat 443.584 kb on - strandat 443.585 kb on + strandat 443.742 kb on + strand, within MMJJ_RS02345at 443.807 kb on - strand, within MMJJ_RS02345at 443.807 kb on - strand, within MMJJ_RS02345at 443.808 kb on + strand, within MMJJ_RS02345at 444.001 kb on + strand, within MMJJ_RS02345at 444.001 kb on + strand, within MMJJ_RS02345at 444.007 kb on + strand, within MMJJ_RS02345at 444.030 kb on - strand, within MMJJ_RS02345at 444.059 kb on + strand, within MMJJ_RS02345at 444.059 kb on + strand, within MMJJ_RS02345at 444.073 kb on - strand, within MMJJ_RS02345at 444.104 kb on - strand, within MMJJ_RS02345at 444.105 kb on + strand, within MMJJ_RS02345at 444.123 kb on - strand, within MMJJ_RS02345at 444.125 kb on - strand, within MMJJ_RS02345at 444.126 kb on + strand, within MMJJ_RS02345at 444.135 kb on - strand, within MMJJ_RS02345at 444.143 kb on - strand, within MMJJ_RS02345at 444.209 kb on + strand, within MMJJ_RS02345at 444.225 kb on - strand, within MMJJ_RS02345at 444.225 kb on - strand, within MMJJ_RS02345at 444.238 kb on + strand, within MMJJ_RS02345at 444.238 kb on + strand, within MMJJ_RS02345at 444.275 kb on + strand, within MMJJ_RS02345at 444.275 kb on + strand, within MMJJ_RS02345at 444.282 kb on - strand, within MMJJ_RS02345at 444.293 kb on - strand, within MMJJ_RS02345at 444.317 kb on + strand, within MMJJ_RS02345at 444.328 kb on - strand, within MMJJ_RS02345at 444.328 kb on - strand, within MMJJ_RS02345at 444.329 kb on + strand, within MMJJ_RS02345at 444.402 kb on - strand, within MMJJ_RS02345at 444.433 kb on + strand, within MMJJ_RS02345at 444.490 kb on + strand, within MMJJ_RS02345at 444.517 kb on - strand, within MMJJ_RS02345at 444.554 kb on + strand, within MMJJ_RS02345at 444.682 kb on - strandat 444.682 kb on - strandat 444.683 kb on + strandat 444.697 kb on - strandat 444.704 kb on + strandat 444.704 kb on + strandat 444.719 kb on - strandat 444.726 kb on - strandat 444.837 kb on + strandat 444.873 kb on - strandat 444.873 kb on - strandat 444.878 kb on - strandat 444.899 kb on - strandat 444.918 kb on + strandat 444.919 kb on - strandat 444.920 kb on + strandat 444.956 kb on + strandat 444.962 kb on - strandat 445.021 kb on - strandat 445.045 kb on + strandat 445.052 kb on + strandat 445.064 kb on - strandat 445.102 kb on - strand, within MMJJ_RS02350at 445.130 kb on + strand, within MMJJ_RS02350at 445.153 kb on - strand, within MMJJ_RS02350at 445.198 kb on - strand, within MMJJ_RS02350at 445.199 kb on + strand, within MMJJ_RS02350at 445.203 kb on + strand, within MMJJ_RS02350at 445.308 kb on + strand, within MMJJ_RS02350at 445.309 kb on - strand, within MMJJ_RS02350at 445.309 kb on - strand, within MMJJ_RS02350at 445.309 kb on - strand, within MMJJ_RS02350at 445.309 kb on - strand, within MMJJ_RS02350at 445.309 kb on - strand, within MMJJ_RS02350at 445.310 kb on + strand, within MMJJ_RS02350at 445.310 kb on + strand, within MMJJ_RS02350at 445.310 kb on + strand, within MMJJ_RS02350at 445.310 kb on + strand, within MMJJ_RS02350at 445.310 kb on + strand, within MMJJ_RS02350at 445.314 kb on - strand, within MMJJ_RS02350at 445.315 kb on + strand, within MMJJ_RS02350at 445.369 kb on - strand, within MMJJ_RS02350at 445.370 kb on + strand, within MMJJ_RS02350at 445.464 kb on + strand, within MMJJ_RS02350at 445.484 kb on + strand, within MMJJ_RS02350at 445.595 kb on - strand, within MMJJ_RS02350at 445.634 kb on - strand, within MMJJ_RS02350at 445.665 kb on - strand, within MMJJ_RS02350at 445.665 kb on - strand, within MMJJ_RS02350

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
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442,532 + -2.2
442,536 + +3.8
442,536 + -1.4
442,546 + -1.0
442,592 + +0.9
442,614 - +1.4
442,619 - -3.9
442,620 + -3.7
442,670 + MMJJ_RS02340 0.15 +1.4
442,670 + MMJJ_RS02340 0.15 +0.9
442,740 - MMJJ_RS02340 0.27 -1.3
442,740 - MMJJ_RS02340 0.27 +1.9
442,740 - MMJJ_RS02340 0.27 -0.4
442,740 - +1.7
442,740 - MMJJ_RS02340 0.27 -1.7
442,741 + MMJJ_RS02340 0.28 +2.2
442,741 + MMJJ_RS02340 0.28 +1.9
442,741 + MMJJ_RS02340 0.28 +3.1
442,741 + MMJJ_RS02340 0.28 +2.0
442,741 + MMJJ_RS02340 0.28 +2.1
442,741 + MMJJ_RS02340 0.28 +3.9
442,741 + MMJJ_RS02340 0.28 +4.7
442,741 + MMJJ_RS02340 0.28 -1.0
442,765 + MMJJ_RS02340 0.32 +0.7
442,765 + MMJJ_RS02340 0.32 -3.3
442,906 - MMJJ_RS02340 0.58 +1.3
442,906 - MMJJ_RS02340 0.58 +2.3
442,906 - MMJJ_RS02340 0.58 -2.3
442,907 + MMJJ_RS02340 0.58 -0.4
443,078 - MMJJ_RS02340 0.89 -1.3
443,079 + MMJJ_RS02340 0.89 -1.8
443,082 + MMJJ_RS02340 0.90 +2.7
443,082 + MMJJ_RS02340 0.90 +0.2
443,214 + -1.4
443,218 - +3.7
443,236 - +2.2
443,236 - -0.4
443,237 + +0.7
443,238 - +0.6
443,238 - -2.2
443,239 + -2.9
443,240 - -2.4
443,240 - -1.5
443,288 + +1.5
443,301 + -1.9
443,337 - -3.2
443,350 - -1.0
443,354 - -0.9
443,355 + +1.1
443,384 - -3.2
443,384 - -1.9
443,394 + +2.7
443,398 - +0.3
443,402 - -1.8
443,463 - -1.7
443,463 - +0.4
443,463 - +1.2
443,463 - -3.7
443,463 - +1.8
443,464 + -0.9
443,464 + +0.0
443,464 + -2.1
443,464 + +1.7
443,464 + -1.7
443,464 + -1.1
443,465 - +0.2
443,465 - -0.8
443,465 - -0.5
443,465 - +1.0
443,466 + +1.7
443,466 + -2.4
443,467 - -2.8
443,467 - +1.8
443,468 + -0.3
443,468 + +3.1
443,468 + +0.1
443,472 + +3.7
443,490 + +0.8
443,494 + -1.4
443,537 + +0.2
443,538 - -1.7
443,539 + +0.3
443,564 + +2.1
443,583 + +2.0
443,584 - +3.9
443,585 + +3.0
443,742 + MMJJ_RS02345 0.20 -2.7
443,807 - MMJJ_RS02345 0.25 -0.5
443,807 - MMJJ_RS02345 0.25 -3.5
443,808 + MMJJ_RS02345 0.25 +1.7
444,001 + MMJJ_RS02345 0.40 +2.7
444,001 + MMJJ_RS02345 0.40 +0.1
444,007 + MMJJ_RS02345 0.41 -0.8
444,030 - MMJJ_RS02345 0.43 +0.6
444,059 + MMJJ_RS02345 0.45 -1.6
444,059 + MMJJ_RS02345 0.45 +4.6
444,073 - MMJJ_RS02345 0.46 -3.3
444,104 - MMJJ_RS02345 0.48 +1.2
444,105 + MMJJ_RS02345 0.49 -1.6
444,123 - MMJJ_RS02345 0.50 -0.4
444,125 - MMJJ_RS02345 0.50 -0.7
444,126 + MMJJ_RS02345 0.50 +2.6
444,135 - MMJJ_RS02345 0.51 +2.9
444,143 - MMJJ_RS02345 0.51 +4.1
444,209 + MMJJ_RS02345 0.57 +1.2
444,225 - MMJJ_RS02345 0.58 -0.1
444,225 - MMJJ_RS02345 0.58 +2.0
444,238 + MMJJ_RS02345 0.59 +3.3
444,238 + MMJJ_RS02345 0.59 +2.2
444,275 + MMJJ_RS02345 0.62 +2.5
444,275 + MMJJ_RS02345 0.62 +0.0
444,282 - MMJJ_RS02345 0.62 -1.7
444,293 - MMJJ_RS02345 0.63 -2.8
444,317 + MMJJ_RS02345 0.65 -1.2
444,328 - MMJJ_RS02345 0.66 -2.8
444,328 - MMJJ_RS02345 0.66 +3.3
444,329 + MMJJ_RS02345 0.66 +5.8
444,402 - MMJJ_RS02345 0.72 -0.7
444,433 + MMJJ_RS02345 0.74 -0.7
444,490 + MMJJ_RS02345 0.79 +0.8
444,517 - MMJJ_RS02345 0.81 -1.2
444,554 + MMJJ_RS02345 0.84 -1.5
444,682 - +1.0
444,682 - +2.6
444,683 + +2.2
444,697 - -2.4
444,704 + +2.6
444,704 + -2.8
444,719 - +0.2
444,726 - -1.0
444,837 + -3.3
444,873 - +1.3
444,873 - +1.5
444,878 - +1.1
444,899 - +0.1
444,918 + +1.5
444,919 - -2.0
444,920 + +0.2
444,956 + -3.1
444,962 - -1.4
445,021 - +0.7
445,045 + -2.3
445,052 + +3.0
445,064 - +1.7
445,102 - MMJJ_RS02350 0.10 +0.7
445,130 + MMJJ_RS02350 0.13 -1.1
445,153 - MMJJ_RS02350 0.14 -1.5
445,198 - MMJJ_RS02350 0.18 +2.2
445,199 + MMJJ_RS02350 0.18 -0.1
445,203 + MMJJ_RS02350 0.18 +0.9
445,308 + MMJJ_RS02350 0.26 -0.4
445,309 - MMJJ_RS02350 0.26 +0.2
445,309 - MMJJ_RS02350 0.26 +3.7
445,309 - MMJJ_RS02350 0.26 +2.8
445,309 - MMJJ_RS02350 0.26 +3.4
445,309 - MMJJ_RS02350 0.26 +0.6
445,310 + MMJJ_RS02350 0.27 -1.0
445,310 + MMJJ_RS02350 0.27 +1.4
445,310 + MMJJ_RS02350 0.27 -1.5
445,310 + MMJJ_RS02350 0.27 -0.3
445,310 + MMJJ_RS02350 0.27 -1.2
445,314 - MMJJ_RS02350 0.27 -3.7
445,315 + MMJJ_RS02350 0.27 +1.5
445,369 - MMJJ_RS02350 0.31 +3.2
445,370 + MMJJ_RS02350 0.31 -1.0
445,464 + MMJJ_RS02350 0.38 +0.7
445,484 + MMJJ_RS02350 0.40 -0.4
445,595 - MMJJ_RS02350 0.49 +3.7
445,634 - MMJJ_RS02350 0.52 -0.5
445,665 - MMJJ_RS02350 0.54 -2.5
445,665 - MMJJ_RS02350 0.54 +1.8

Or see this region's nucleotide sequence