Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02250 and MMJJ_RS02255 are separated by 4 nucleotides MMJJ_RS02255 and MMJJ_RS02260 are separated by 5 nucleotides MMJJ_RS02260 and MMJJ_RS02265 are separated by 120 nucleotides MMJJ_RS02265 and MMJJ_RS02270 are separated by 157 nucleotides MMJJ_RS02270 and MMJJ_RS02275 overlap by 14 nucleotides
MMJJ_RS02250: MMJJ_RS02250 - mechanosensitive ion channel family protein, at 425,846 to 426,928
_RS02250
MMJJ_RS02255: MMJJ_RS02255 - hypothetical protein, at 426,933 to 427,163
_RS02255
MMJJ_RS02260: MMJJ_RS02260 - hypothetical protein, at 427,169 to 427,738
_RS02260
MMJJ_RS02265: MMJJ_RS02265 - hypothetical protein, at 427,859 to 429,328
_RS02265
MMJJ_RS02270: MMJJ_RS02270 - HEPN domain-containing protein, at 429,486 to 429,995
_RS02270
MMJJ_RS02275: MMJJ_RS02275 - nucleotidyltransferase domain-containing protein, at 429,982 to 430,599
_RS02275
Position (kb) 427
428
429
430 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 427.146 kb on + strand at 427.164 kb on + strand at 427.255 kb on - strand, within MMJJ_RS02260 at 427.256 kb on + strand, within MMJJ_RS02260 at 427.272 kb on + strand, within MMJJ_RS02260 at 427.274 kb on - strand, within MMJJ_RS02260 at 427.275 kb on + strand, within MMJJ_RS02260 at 427.275 kb on + strand, within MMJJ_RS02260 at 427.280 kb on - strand, within MMJJ_RS02260 at 427.280 kb on - strand, within MMJJ_RS02260 at 427.293 kb on + strand, within MMJJ_RS02260 at 427.293 kb on + strand, within MMJJ_RS02260 at 427.294 kb on - strand, within MMJJ_RS02260 at 427.295 kb on + strand, within MMJJ_RS02260 at 427.298 kb on - strand, within MMJJ_RS02260 at 427.298 kb on - strand, within MMJJ_RS02260 at 427.301 kb on - strand, within MMJJ_RS02260 at 427.302 kb on + strand, within MMJJ_RS02260 at 427.362 kb on - strand, within MMJJ_RS02260 at 427.362 kb on - strand, within MMJJ_RS02260 at 427.363 kb on + strand, within MMJJ_RS02260 at 427.363 kb on + strand, within MMJJ_RS02260 at 427.363 kb on + strand, within MMJJ_RS02260 at 427.363 kb on + strand, within MMJJ_RS02260 at 427.371 kb on + strand, within MMJJ_RS02260 at 427.371 kb on + strand, within MMJJ_RS02260 at 427.379 kb on - strand, within MMJJ_RS02260 at 427.380 kb on + strand, within MMJJ_RS02260 at 427.380 kb on + strand, within MMJJ_RS02260 at 427.383 kb on + strand, within MMJJ_RS02260 at 427.383 kb on + strand, within MMJJ_RS02260 at 427.383 kb on + strand, within MMJJ_RS02260 at 427.392 kb on + strand, within MMJJ_RS02260 at 427.392 kb on + strand, within MMJJ_RS02260 at 427.392 kb on + strand, within MMJJ_RS02260 at 427.436 kb on - strand, within MMJJ_RS02260 at 427.488 kb on + strand, within MMJJ_RS02260 at 427.622 kb on - strand, within MMJJ_RS02260 at 427.625 kb on - strand, within MMJJ_RS02260 at 427.626 kb on + strand, within MMJJ_RS02260 at 427.643 kb on - strand, within MMJJ_RS02260 at 427.644 kb on + strand, within MMJJ_RS02260 at 427.673 kb on - strand, within MMJJ_RS02260 at 427.673 kb on - strand, within MMJJ_RS02260 at 427.716 kb on - strand at 427.723 kb on - strand at 427.724 kb on + strand at 427.738 kb on - strand at 427.739 kb on + strand at 427.739 kb on + strand at 427.755 kb on + strand at 427.763 kb on - strand at 427.763 kb on - strand at 427.768 kb on - strand at 427.769 kb on + strand at 427.769 kb on + strand at 427.839 kb on - strand at 427.889 kb on - strand at 427.910 kb on - strand at 428.055 kb on + strand, within MMJJ_RS02265 at 428.168 kb on - strand, within MMJJ_RS02265 at 428.170 kb on - strand, within MMJJ_RS02265 at 428.173 kb on - strand, within MMJJ_RS02265 at 428.178 kb on + strand, within MMJJ_RS02265 at 428.200 kb on - strand, within MMJJ_RS02265 at 428.201 kb on + strand, within MMJJ_RS02265 at 428.202 kb on - strand, within MMJJ_RS02265 at 428.318 kb on + strand, within MMJJ_RS02265 at 428.358 kb on + strand, within MMJJ_RS02265 at 428.416 kb on + strand, within MMJJ_RS02265 at 428.417 kb on + strand, within MMJJ_RS02265 at 428.450 kb on - strand, within MMJJ_RS02265 at 428.451 kb on + strand, within MMJJ_RS02265 at 428.453 kb on - strand, within MMJJ_RS02265 at 428.483 kb on - strand, within MMJJ_RS02265 at 428.484 kb on + strand, within MMJJ_RS02265 at 428.484 kb on + strand, within MMJJ_RS02265 at 428.540 kb on - strand, within MMJJ_RS02265 at 428.574 kb on + strand, within MMJJ_RS02265 at 428.574 kb on + strand, within MMJJ_RS02265 at 428.669 kb on + strand, within MMJJ_RS02265 at 428.671 kb on + strand, within MMJJ_RS02265 at 428.837 kb on - strand, within MMJJ_RS02265 at 428.837 kb on - strand, within MMJJ_RS02265 at 428.838 kb on + strand, within MMJJ_RS02265 at 428.838 kb on + strand, within MMJJ_RS02265 at 428.838 kb on + strand, within MMJJ_RS02265 at 428.844 kb on - strand, within MMJJ_RS02265 at 428.844 kb on - strand, within MMJJ_RS02265 at 428.845 kb on + strand, within MMJJ_RS02265 at 428.846 kb on - strand, within MMJJ_RS02265 at 428.875 kb on - strand, within MMJJ_RS02265 at 428.876 kb on + strand, within MMJJ_RS02265 at 428.876 kb on + strand, within MMJJ_RS02265 at 428.877 kb on - strand, within MMJJ_RS02265 at 428.877 kb on - strand, within MMJJ_RS02265 at 428.878 kb on + strand, within MMJJ_RS02265 at 428.878 kb on + strand, within MMJJ_RS02265 at 428.878 kb on + strand, within MMJJ_RS02265 at 428.880 kb on + strand, within MMJJ_RS02265 at 428.880 kb on + strand, within MMJJ_RS02265 at 428.882 kb on - strand, within MMJJ_RS02265 at 428.882 kb on - strand, within MMJJ_RS02265 at 428.883 kb on + strand, within MMJJ_RS02265 at 428.933 kb on - strand, within MMJJ_RS02265 at 428.933 kb on - strand, within MMJJ_RS02265 at 428.934 kb on + strand, within MMJJ_RS02265 at 428.988 kb on - strand, within MMJJ_RS02265 at 429.030 kb on + strand, within MMJJ_RS02265 at 429.125 kb on - strand, within MMJJ_RS02265 at 429.181 kb on - strand, within MMJJ_RS02265 at 429.235 kb on - strand at 429.289 kb on - strand at 429.302 kb on + strand at 429.309 kb on + strand at 429.331 kb on + strand at 429.357 kb on - strand at 429.357 kb on - strand at 429.357 kb on - strand at 429.357 kb on - strand at 429.358 kb on + strand at 429.358 kb on + strand at 429.359 kb on - strand at 429.359 kb on - strand at 429.360 kb on + strand at 429.365 kb on - strand at 429.365 kb on - strand at 429.365 kb on - strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.366 kb on + strand at 429.382 kb on - strand at 429.383 kb on + strand at 429.383 kb on + strand at 429.394 kb on + strand at 429.406 kb on - strand at 429.410 kb on - strand at 429.423 kb on + strand at 429.433 kb on + strand at 429.435 kb on + strand at 429.458 kb on + strand at 429.516 kb on - strand at 429.546 kb on - strand, within MMJJ_RS02270 at 429.547 kb on + strand, within MMJJ_RS02270 at 429.610 kb on + strand, within MMJJ_RS02270 at 429.638 kb on - strand, within MMJJ_RS02270 at 429.703 kb on + strand, within MMJJ_RS02270 at 429.803 kb on - strand, within MMJJ_RS02270 at 429.843 kb on - strand, within MMJJ_RS02270 at 429.858 kb on + strand, within MMJJ_RS02270 at 429.943 kb on - strand, within MMJJ_RS02270 at 429.976 kb on - strand at 430.109 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.111 kb on + strand, within MMJJ_RS02275 at 430.114 kb on - strand, within MMJJ_RS02275
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM remove 427,146 + -0.4 427,164 + +0.9 427,255 - MMJJ_RS02260 0.15 -0.6 427,256 + MMJJ_RS02260 0.15 +2.3 427,272 + MMJJ_RS02260 0.18 -0.7 427,274 - MMJJ_RS02260 0.18 +2.3 427,275 + MMJJ_RS02260 0.19 -3.5 427,275 + MMJJ_RS02260 0.19 +0.3 427,280 - MMJJ_RS02260 0.19 -3.3 427,280 - MMJJ_RS02260 0.19 -2.6 427,293 + MMJJ_RS02260 0.22 -1.2 427,293 + MMJJ_RS02260 0.22 -4.6 427,294 - MMJJ_RS02260 0.22 +0.6 427,295 + MMJJ_RS02260 0.22 -4.2 427,298 - MMJJ_RS02260 0.23 +1.2 427,298 - MMJJ_RS02260 0.23 +1.7 427,301 - MMJJ_RS02260 0.23 +2.6 427,302 + MMJJ_RS02260 0.23 +2.5 427,362 - MMJJ_RS02260 0.34 +0.6 427,362 - MMJJ_RS02260 0.34 +1.2 427,363 + MMJJ_RS02260 0.34 -1.6 427,363 + MMJJ_RS02260 0.34 +0.6 427,363 + MMJJ_RS02260 0.34 -2.5 427,363 + MMJJ_RS02260 0.34 -2.6 427,371 + MMJJ_RS02260 0.35 -2.1 427,371 + MMJJ_RS02260 0.35 +2.5 427,379 - MMJJ_RS02260 0.37 +1.7 427,380 + MMJJ_RS02260 0.37 +4.3 427,380 + MMJJ_RS02260 0.37 -1.9 427,383 + MMJJ_RS02260 0.38 -2.1 427,383 + MMJJ_RS02260 0.38 -2.6 427,383 + MMJJ_RS02260 0.38 +1.0 427,392 + MMJJ_RS02260 0.39 -0.4 427,392 + MMJJ_RS02260 0.39 +3.9 427,392 + MMJJ_RS02260 0.39 -1.2 427,436 - MMJJ_RS02260 0.47 +3.0 427,488 + MMJJ_RS02260 0.56 +2.0 427,622 - MMJJ_RS02260 0.79 +0.0 427,625 - MMJJ_RS02260 0.80 +0.6 427,626 + MMJJ_RS02260 0.80 +1.0 427,643 - MMJJ_RS02260 0.83 +1.4 427,644 + MMJJ_RS02260 0.83 +1.2 427,673 - MMJJ_RS02260 0.88 -0.0 427,673 - MMJJ_RS02260 0.88 -0.5 427,716 - -0.4 427,723 - +1.0 427,724 + -0.2 427,738 - +1.4 427,739 + -2.4 427,739 + -0.4 427,755 + +2.5 427,763 - -2.9 427,763 - -0.5 427,768 - -2.1 427,769 + +1.9 427,769 + -2.6 427,839 - +3.8 427,889 - +1.3 427,910 - -0.5 428,055 + MMJJ_RS02265 0.13 -2.3 428,168 - MMJJ_RS02265 0.21 -2.9 428,170 - MMJJ_RS02265 0.21 +2.9 428,173 - MMJJ_RS02265 0.21 +2.3 428,178 + MMJJ_RS02265 0.22 -0.1 428,200 - MMJJ_RS02265 0.23 -2.7 428,201 + MMJJ_RS02265 0.23 +1.2 428,202 - MMJJ_RS02265 0.23 +0.6 428,318 + MMJJ_RS02265 0.31 +1.0 428,358 + MMJJ_RS02265 0.34 -0.8 428,416 + MMJJ_RS02265 0.38 +1.2 428,417 + MMJJ_RS02265 0.38 +1.4 428,450 - MMJJ_RS02265 0.40 +2.6 428,451 + MMJJ_RS02265 0.40 +2.3 428,453 - MMJJ_RS02265 0.40 -1.8 428,483 - MMJJ_RS02265 0.42 +0.6 428,484 + MMJJ_RS02265 0.43 +1.3 428,484 + MMJJ_RS02265 0.43 +3.9 428,540 - MMJJ_RS02265 0.46 +1.8 428,574 + MMJJ_RS02265 0.49 +0.2 428,574 + MMJJ_RS02265 0.49 +1.3 428,669 + MMJJ_RS02265 0.55 -0.9 428,671 + MMJJ_RS02265 0.55 +0.2 428,837 - MMJJ_RS02265 0.67 +0.1 428,837 - MMJJ_RS02265 0.67 -0.1 428,838 + MMJJ_RS02265 0.67 +1.0 428,838 + MMJJ_RS02265 0.67 +4.3 428,838 + MMJJ_RS02265 0.67 -2.9 428,844 - MMJJ_RS02265 0.67 +5.3 428,844 - MMJJ_RS02265 0.67 +2.2 428,845 + MMJJ_RS02265 0.67 +0.1 428,846 - MMJJ_RS02265 0.67 +1.0 428,875 - MMJJ_RS02265 0.69 -3.9 428,876 + MMJJ_RS02265 0.69 -2.3 428,876 + MMJJ_RS02265 0.69 +0.1 428,877 - MMJJ_RS02265 0.69 +3.3 428,877 - MMJJ_RS02265 0.69 +0.8 428,878 + MMJJ_RS02265 0.69 -2.9 428,878 + MMJJ_RS02265 0.69 +3.1 428,878 + MMJJ_RS02265 0.69 -3.4 428,880 + MMJJ_RS02265 0.69 -2.9 428,880 + MMJJ_RS02265 0.69 +2.2 428,882 - MMJJ_RS02265 0.70 -2.1 428,882 - MMJJ_RS02265 0.70 +2.0 428,883 + MMJJ_RS02265 0.70 -2.3 428,933 - MMJJ_RS02265 0.73 -1.5 428,933 - MMJJ_RS02265 0.73 -0.7 428,934 + MMJJ_RS02265 0.73 -1.6 428,988 - MMJJ_RS02265 0.77 +0.0 429,030 + MMJJ_RS02265 0.80 -1.9 429,125 - MMJJ_RS02265 0.86 +2.3 429,181 - MMJJ_RS02265 0.90 +0.7 429,235 - -0.6 429,289 - -1.9 429,302 + +0.0 429,309 + -2.8 429,331 + +1.4 429,357 - +2.9 429,357 - +2.8 429,357 - +1.2 429,357 - +0.0 429,358 + -1.6 429,358 + +0.3 429,359 - +1.5 429,359 - +2.7 429,360 + +2.1 429,365 - -1.4 429,365 - -1.1 429,365 - -1.8 429,366 + -2.6 429,366 + +3.0 429,366 + -3.1 429,366 + -3.2 429,366 + +2.6 429,382 - -2.7 429,383 + -1.0 429,383 + -2.1 429,394 + -1.3 429,406 - -2.2 429,410 - -0.2 429,423 + +2.4 429,433 + +0.7 429,435 + -2.6 429,458 + +3.1 429,516 - +1.8 429,546 - MMJJ_RS02270 0.12 +1.6 429,547 + MMJJ_RS02270 0.12 +0.2 429,610 + MMJJ_RS02270 0.24 -2.6 429,638 - MMJJ_RS02270 0.30 -2.8 429,703 + MMJJ_RS02270 0.43 -2.2 429,803 - MMJJ_RS02270 0.62 +1.6 429,843 - MMJJ_RS02270 0.70 -0.2 429,858 + MMJJ_RS02270 0.73 -4.1 429,943 - MMJJ_RS02270 0.90 +1.0 429,976 - -2.5 430,109 + MMJJ_RS02275 0.21 +1.1 430,111 + MMJJ_RS02275 0.21 -2.1 430,111 + MMJJ_RS02275 0.21 +0.4 430,111 + MMJJ_RS02275 0.21 +0.4 430,114 - MMJJ_RS02275 0.21 -2.8
Or see this region's nucleotide sequence