Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01875

Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01870 and MMJJ_RS01875 are separated by 36 nucleotidesMMJJ_RS01875 and MMJJ_RS01880 are separated by 22 nucleotidesMMJJ_RS01880 and MMJJ_RS01885 are separated by 52 nucleotides MMJJ_RS01870: MMJJ_RS01870 - methanogenesis marker 15 protein, at 358,786 to 360,021 _RS01870 MMJJ_RS01875: MMJJ_RS01875 - global nitrogen regulator NrpR, at 360,058 to 361,668 _RS01875 MMJJ_RS01880: MMJJ_RS01880 - RlmE family RNA methyltransferase, at 361,691 to 362,467 _RS01880 MMJJ_RS01885: MMJJ_RS01885 - KH domain-containing protein, at 362,520 to 363,074 _RS01885 Position (kb) 360 361 362Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 359.061 kb on + strandat 359.082 kb on + strand, within MMJJ_RS01870at 359.528 kb on - strand, within MMJJ_RS01870at 359.528 kb on - strand, within MMJJ_RS01870at 359.733 kb on + strand, within MMJJ_RS01870at 359.855 kb on - strand, within MMJJ_RS01870at 359.856 kb on + strandat 359.965 kb on + strandat 360.045 kb on - strandat 360.045 kb on - strandat 360.046 kb on + strandat 360.046 kb on + strandat 360.046 kb on + strandat 360.046 kb on + strandat 360.046 kb on + strandat 360.158 kb on + strandat 360.191 kb on - strandat 360.294 kb on - strand, within MMJJ_RS01875at 360.414 kb on + strand, within MMJJ_RS01875at 360.437 kb on + strandat 360.477 kb on - strand, within MMJJ_RS01875at 360.477 kb on - strand, within MMJJ_RS01875at 360.478 kb on + strand, within MMJJ_RS01875at 360.488 kb on - strand, within MMJJ_RS01875at 360.501 kb on - strand, within MMJJ_RS01875at 360.501 kb on - strand, within MMJJ_RS01875at 360.540 kb on + strand, within MMJJ_RS01875at 360.540 kb on + strand, within MMJJ_RS01875at 360.651 kb on - strand, within MMJJ_RS01875at 360.675 kb on + strand, within MMJJ_RS01875at 360.900 kb on - strand, within MMJJ_RS01875at 360.911 kb on - strand, within MMJJ_RS01875at 360.912 kb on + strand, within MMJJ_RS01875at 360.912 kb on + strand, within MMJJ_RS01875at 361.046 kb on - strand, within MMJJ_RS01875at 361.085 kb on - strandat 361.112 kb on + strand, within MMJJ_RS01875at 361.245 kb on + strandat 361.449 kb on + strand, within MMJJ_RS01875at 361.679 kb on + strandat 361.740 kb on + strandat 361.743 kb on + strandat 361.785 kb on + strand, within MMJJ_RS01880at 361.848 kb on - strand, within MMJJ_RS01880at 362.107 kb on - strand, within MMJJ_RS01880at 362.278 kb on + strand, within MMJJ_RS01880at 362.280 kb on + strand, within MMJJ_RS01880at 362.340 kb on - strand, within MMJJ_RS01880at 362.341 kb on + strand, within MMJJ_RS01880at 362.478 kb on + strandat 362.481 kb on + strandat 362.482 kb on - strandat 362.491 kb on - strandat 362.491 kb on - strandat 362.492 kb on + strandat 362.505 kb on + strandat 362.516 kb on + strandat 362.516 kb on + strandat 362.522 kb on + strandat 362.535 kb on + strandat 362.550 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
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359,061 + -0.1
359,082 + MMJJ_RS01870 0.24 -0.9
359,528 - MMJJ_RS01870 0.60 +2.5
359,528 - MMJJ_RS01870 0.60 -0.3
359,733 + MMJJ_RS01870 0.77 +1.5
359,855 - MMJJ_RS01870 0.86 +0.2
359,856 + +2.5
359,965 + -0.3
360,045 - +0.2
360,045 - +0.2
360,046 + +0.5
360,046 + +0.2
360,046 + -0.9
360,046 + -1.6
360,046 + -0.5
360,158 + -1.7
360,191 - -1.1
360,294 - MMJJ_RS01875 0.15 +0.5
360,414 + MMJJ_RS01875 0.22 -1.2
360,437 + +1.9
360,477 - MMJJ_RS01875 0.26 -0.1
360,477 - MMJJ_RS01875 0.26 -1.2
360,478 + MMJJ_RS01875 0.26 -0.1
360,488 - MMJJ_RS01875 0.27 -0.1
360,501 - MMJJ_RS01875 0.27 -2.2
360,501 - MMJJ_RS01875 0.27 -0.7
360,540 + MMJJ_RS01875 0.30 -0.3
360,540 + MMJJ_RS01875 0.30 -0.1
360,651 - MMJJ_RS01875 0.37 -3.3
360,675 + MMJJ_RS01875 0.38 -1.1
360,900 - MMJJ_RS01875 0.52 +0.4
360,911 - MMJJ_RS01875 0.53 -0.5
360,912 + MMJJ_RS01875 0.53 -1.2
360,912 + MMJJ_RS01875 0.53 -1.3
361,046 - MMJJ_RS01875 0.61 -0.1
361,085 - +2.5
361,112 + MMJJ_RS01875 0.65 +0.2
361,245 + +1.5
361,449 + MMJJ_RS01875 0.86 -1.4
361,679 + -2.0
361,740 + -2.1
361,743 + -1.2
361,785 + MMJJ_RS01880 0.12 +1.3
361,848 - MMJJ_RS01880 0.20 -1.2
362,107 - MMJJ_RS01880 0.54 -2.2
362,278 + MMJJ_RS01880 0.76 -1.6
362,280 + MMJJ_RS01880 0.76 +0.9
362,340 - MMJJ_RS01880 0.84 -2.0
362,341 + MMJJ_RS01880 0.84 -1.7
362,478 + -2.9
362,481 + +1.6
362,482 - -3.5
362,491 - -3.6
362,491 - -1.8
362,492 + -0.8
362,505 + -3.8
362,516 + -0.3
362,516 + +0.5
362,522 + +2.8
362,535 + +0.2
362,550 + -3.0

Or see this region's nucleotide sequence