Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01730 and MMJJ_RS01735 are separated by 154 nucleotides MMJJ_RS01735 and MMJJ_RS01740 are separated by 133 nucleotides MMJJ_RS01740 and MMJJ_RS01745 are separated by 181 nucleotides MMJJ_RS01745 and MMJJ_RS01750 are separated by 111 nucleotides
MMJJ_RS01730: MMJJ_RS01730 - ABC transporter permease, at 334,820 to 336,013
_RS01730
MMJJ_RS01735: MMJJ_RS01735 - rubrerythrin family protein, at 336,168 to 336,758
_RS01735
MMJJ_RS01740: MMJJ_RS01740 - ATP-binding protein, at 336,892 to 338,058
_RS01740
MMJJ_RS01745: MMJJ_RS01745 - metal-dependent transcriptional regulator, at 338,240 to 338,645
_RS01745
MMJJ_RS01750: MMJJ_RS01750 - ferrous iron transport protein A, at 338,757 to 338,963
_RS01750
Position (kb) 336
337
338
339 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 335.905 kb on - strand at 335.905 kb on - strand at 335.909 kb on - strand at 335.910 kb on + strand at 335.910 kb on + strand at 335.910 kb on + strand at 335.924 kb on - strand at 335.972 kb on - strand at 335.972 kb on - strand at 335.973 kb on + strand at 336.053 kb on + strand at 336.067 kb on + strand at 336.081 kb on + strand at 336.090 kb on + strand at 336.098 kb on - strand at 336.099 kb on + strand at 336.111 kb on + strand at 336.111 kb on + strand at 336.112 kb on - strand at 336.113 kb on + strand at 336.116 kb on + strand at 336.116 kb on + strand at 336.149 kb on + strand at 336.170 kb on + strand at 336.173 kb on + strand at 336.196 kb on - strand at 336.196 kb on - strand at 336.196 kb on - strand at 336.196 kb on - strand at 336.197 kb on + strand at 336.197 kb on + strand at 336.249 kb on - strand, within MMJJ_RS01735 at 336.282 kb on - strand, within MMJJ_RS01735 at 336.282 kb on - strand, within MMJJ_RS01735 at 336.282 kb on - strand, within MMJJ_RS01735 at 336.282 kb on - strand, within MMJJ_RS01735 at 336.282 kb on - strand, within MMJJ_RS01735 at 336.283 kb on + strand, within MMJJ_RS01735 at 336.283 kb on + strand, within MMJJ_RS01735 at 336.330 kb on - strand, within MMJJ_RS01735 at 336.331 kb on + strand, within MMJJ_RS01735 at 336.382 kb on + strand, within MMJJ_RS01735 at 336.493 kb on + strand, within MMJJ_RS01735 at 336.497 kb on - strand, within MMJJ_RS01735 at 336.497 kb on - strand, within MMJJ_RS01735 at 336.497 kb on - strand, within MMJJ_RS01735 at 336.506 kb on + strand, within MMJJ_RS01735 at 336.506 kb on + strand, within MMJJ_RS01735 at 336.512 kb on - strand, within MMJJ_RS01735 at 336.676 kb on - strand, within MMJJ_RS01735 at 336.683 kb on - strand, within MMJJ_RS01735 at 336.683 kb on - strand, within MMJJ_RS01735 at 336.684 kb on + strand, within MMJJ_RS01735 at 336.685 kb on - strand, within MMJJ_RS01735 at 336.690 kb on + strand, within MMJJ_RS01735 at 336.690 kb on + strand, within MMJJ_RS01735 at 336.770 kb on - strand at 336.772 kb on - strand at 336.773 kb on + strand at 336.773 kb on + strand at 336.780 kb on - strand at 336.781 kb on + strand at 336.786 kb on + strand at 336.794 kb on - strand at 336.795 kb on + strand at 336.795 kb on + strand at 336.796 kb on - strand at 336.798 kb on - strand at 336.799 kb on + strand at 336.801 kb on - strand at 336.801 kb on - strand at 336.824 kb on - strand at 336.824 kb on - strand at 336.825 kb on + strand at 336.825 kb on + strand at 336.825 kb on + strand at 336.838 kb on + strand at 336.887 kb on + strand at 337.047 kb on - strand, within MMJJ_RS01740 at 337.076 kb on + strand, within MMJJ_RS01740 at 337.124 kb on + strand, within MMJJ_RS01740 at 337.126 kb on - strand, within MMJJ_RS01740 at 337.143 kb on - strand, within MMJJ_RS01740 at 337.155 kb on + strand, within MMJJ_RS01740 at 337.155 kb on + strand, within MMJJ_RS01740 at 337.212 kb on + strand, within MMJJ_RS01740 at 337.213 kb on - strand, within MMJJ_RS01740 at 337.214 kb on + strand, within MMJJ_RS01740 at 337.264 kb on + strand, within MMJJ_RS01740 at 337.320 kb on - strand, within MMJJ_RS01740 at 337.321 kb on + strand, within MMJJ_RS01740 at 337.393 kb on - strand, within MMJJ_RS01740 at 337.396 kb on + strand, within MMJJ_RS01740 at 337.426 kb on + strand, within MMJJ_RS01740 at 337.518 kb on - strand, within MMJJ_RS01740 at 337.578 kb on - strand, within MMJJ_RS01740 at 337.614 kb on + strand, within MMJJ_RS01740 at 337.683 kb on - strand, within MMJJ_RS01740 at 337.683 kb on - strand, within MMJJ_RS01740 at 337.716 kb on - strand, within MMJJ_RS01740 at 337.717 kb on + strand, within MMJJ_RS01740 at 337.717 kb on + strand, within MMJJ_RS01740 at 337.717 kb on + strand, within MMJJ_RS01740 at 337.718 kb on - strand, within MMJJ_RS01740 at 337.718 kb on - strand, within MMJJ_RS01740 at 337.719 kb on + strand, within MMJJ_RS01740 at 337.719 kb on + strand, within MMJJ_RS01740 at 337.719 kb on + strand, within MMJJ_RS01740 at 337.723 kb on + strand, within MMJJ_RS01740 at 337.723 kb on + strand, within MMJJ_RS01740 at 337.741 kb on - strand, within MMJJ_RS01740 at 337.771 kb on + strand, within MMJJ_RS01740 at 337.771 kb on + strand, within MMJJ_RS01740 at 337.846 kb on + strand, within MMJJ_RS01740 at 337.882 kb on - strand, within MMJJ_RS01740 at 337.882 kb on - strand, within MMJJ_RS01740 at 337.884 kb on - strand, within MMJJ_RS01740 at 337.901 kb on + strand, within MMJJ_RS01740 at 337.905 kb on - strand, within MMJJ_RS01740 at 337.906 kb on + strand, within MMJJ_RS01740 at 337.954 kb on + strand at 338.003 kb on - strand at 338.003 kb on - strand at 338.004 kb on + strand at 338.014 kb on - strand at 338.015 kb on + strand at 338.015 kb on + strand at 338.032 kb on + strand at 338.046 kb on - strand at 338.047 kb on + strand at 338.047 kb on + strand at 338.058 kb on - strand at 338.075 kb on - strand at 338.075 kb on - strand at 338.076 kb on + strand at 338.076 kb on + strand at 338.076 kb on + strand at 338.082 kb on + strand at 338.082 kb on + strand at 338.082 kb on + strand at 338.093 kb on + strand at 338.097 kb on - strand at 338.112 kb on - strand at 338.113 kb on + strand at 338.117 kb on + strand at 338.125 kb on - strand at 338.126 kb on + strand at 338.126 kb on + strand at 338.126 kb on + strand at 338.127 kb on - strand at 338.128 kb on + strand at 338.128 kb on + strand at 338.141 kb on + strand at 338.152 kb on + strand at 338.158 kb on - strand at 338.159 kb on + strand at 338.159 kb on + strand at 338.196 kb on - strand at 338.205 kb on - strand at 338.205 kb on - strand at 338.206 kb on + strand at 338.206 kb on + strand at 338.234 kb on - strand at 338.234 kb on - strand at 338.235 kb on + strand at 338.239 kb on + strand at 338.289 kb on + strand, within MMJJ_RS01745 at 338.298 kb on - strand, within MMJJ_RS01745 at 338.301 kb on + strand, within MMJJ_RS01745 at 338.324 kb on - strand, within MMJJ_RS01745 at 338.324 kb on - strand, within MMJJ_RS01745 at 338.337 kb on + strand, within MMJJ_RS01745 at 338.341 kb on - strand, within MMJJ_RS01745 at 338.341 kb on - strand, within MMJJ_RS01745 at 338.342 kb on + strand, within MMJJ_RS01745 at 338.473 kb on - strand, within MMJJ_RS01745 at 338.482 kb on - strand, within MMJJ_RS01745 at 338.586 kb on + strand, within MMJJ_RS01745 at 338.601 kb on - strand, within MMJJ_RS01745 at 338.620 kb on - strand at 338.630 kb on - strand at 338.631 kb on + strand at 338.666 kb on - strand at 338.666 kb on - strand at 338.667 kb on + strand at 338.667 kb on + strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.668 kb on - strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.669 kb on + strand at 338.670 kb on - strand at 338.671 kb on + strand at 338.673 kb on + strand at 338.737 kb on - strand at 338.740 kb on + strand at 338.741 kb on - strand at 338.791 kb on + strand, within MMJJ_RS01750 at 338.792 kb on - strand, within MMJJ_RS01750 at 338.793 kb on + strand, within MMJJ_RS01750 at 338.797 kb on - strand, within MMJJ_RS01750 at 338.869 kb on - strand, within MMJJ_RS01750 at 338.894 kb on + strand, within MMJJ_RS01750 at 338.956 kb on - strand at 338.962 kb on + strand at 338.962 kb on + strand at 338.983 kb on - strand at 338.992 kb on + strand at 338.994 kb on + strand at 338.994 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 338.999 kb on + strand at 339.003 kb on - strand at 339.018 kb on + strand at 339.046 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM remove 335,905 - -0.1 335,905 - +1.7 335,909 - -1.2 335,910 + +1.5 335,910 + +4.7 335,910 + +1.7 335,924 - -3.1 335,972 - -2.7 335,972 - +0.7 335,973 + +2.2 336,053 + -1.1 336,067 + -3.5 336,081 + +1.6 336,090 + +0.3 336,098 - -1.6 336,099 + -0.0 336,111 + -0.0 336,111 + -3.7 336,112 - -0.9 336,113 + +1.8 336,116 + +3.4 336,116 + -2.0 336,149 + +1.5 336,170 + -1.4 336,173 + -4.9 336,196 - -3.0 336,196 - +1.0 336,196 - +0.7 336,196 - -1.0 336,197 + +0.6 336,197 + -0.3 336,249 - MMJJ_RS01735 0.14 +3.0 336,282 - MMJJ_RS01735 0.19 -0.2 336,282 - MMJJ_RS01735 0.19 +3.0 336,282 - MMJJ_RS01735 0.19 -2.3 336,282 - MMJJ_RS01735 0.19 -1.3 336,282 - MMJJ_RS01735 0.19 -4.1 336,283 + MMJJ_RS01735 0.19 +1.5 336,283 + MMJJ_RS01735 0.19 -2.9 336,330 - MMJJ_RS01735 0.27 +2.2 336,331 + MMJJ_RS01735 0.28 +0.3 336,382 + MMJJ_RS01735 0.36 -2.1 336,493 + MMJJ_RS01735 0.55 +1.4 336,497 - MMJJ_RS01735 0.56 -3.8 336,497 - MMJJ_RS01735 0.56 -0.9 336,497 - MMJJ_RS01735 0.56 +4.6 336,506 + MMJJ_RS01735 0.57 -2.2 336,506 + MMJJ_RS01735 0.57 +0.4 336,512 - MMJJ_RS01735 0.58 -3.2 336,676 - MMJJ_RS01735 0.86 +3.0 336,683 - MMJJ_RS01735 0.87 +2.4 336,683 - MMJJ_RS01735 0.87 -1.9 336,684 + MMJJ_RS01735 0.87 -0.1 336,685 - MMJJ_RS01735 0.87 -1.7 336,690 + MMJJ_RS01735 0.88 -2.2 336,690 + MMJJ_RS01735 0.88 -3.8 336,770 - -0.5 336,772 - +2.9 336,773 + +0.7 336,773 + -0.1 336,780 - +1.1 336,781 + +1.7 336,786 + +1.3 336,794 - +0.3 336,795 + -4.1 336,795 + -2.1 336,796 - -3.2 336,798 - +0.2 336,799 + -3.9 336,801 - -0.5 336,801 - +1.1 336,824 - +3.2 336,824 - +0.1 336,825 + +0.1 336,825 + +1.1 336,825 + -2.3 336,838 + +1.2 336,887 + +2.4 337,047 - MMJJ_RS01740 0.13 +0.6 337,076 + MMJJ_RS01740 0.16 -1.5 337,124 + MMJJ_RS01740 0.20 -0.7 337,126 - MMJJ_RS01740 0.20 +0.7 337,143 - MMJJ_RS01740 0.22 +1.0 337,155 + MMJJ_RS01740 0.23 -1.5 337,155 + MMJJ_RS01740 0.23 -2.7 337,212 + MMJJ_RS01740 0.27 +2.0 337,213 - MMJJ_RS01740 0.28 +2.8 337,214 + MMJJ_RS01740 0.28 +0.7 337,264 + MMJJ_RS01740 0.32 +1.8 337,320 - MMJJ_RS01740 0.37 -4.0 337,321 + MMJJ_RS01740 0.37 +0.2 337,393 - MMJJ_RS01740 0.43 -0.3 337,396 + MMJJ_RS01740 0.43 -2.4 337,426 + MMJJ_RS01740 0.46 -1.9 337,518 - MMJJ_RS01740 0.54 +0.2 337,578 - MMJJ_RS01740 0.59 -3.5 337,614 + MMJJ_RS01740 0.62 -1.7 337,683 - MMJJ_RS01740 0.68 -2.3 337,683 - MMJJ_RS01740 0.68 +1.7 337,716 - MMJJ_RS01740 0.71 -2.8 337,717 + MMJJ_RS01740 0.71 +1.3 337,717 + MMJJ_RS01740 0.71 +1.1 337,717 + MMJJ_RS01740 0.71 -1.7 337,718 - MMJJ_RS01740 0.71 +1.0 337,718 - MMJJ_RS01740 0.71 -1.9 337,719 + MMJJ_RS01740 0.71 -1.3 337,719 + MMJJ_RS01740 0.71 +2.0 337,719 + MMJJ_RS01740 0.71 -2.4 337,723 + MMJJ_RS01740 0.71 -2.5 337,723 + MMJJ_RS01740 0.71 -0.2 337,741 - MMJJ_RS01740 0.73 -1.0 337,771 + MMJJ_RS01740 0.75 -2.2 337,771 + MMJJ_RS01740 0.75 +0.1 337,846 + MMJJ_RS01740 0.82 +2.7 337,882 - MMJJ_RS01740 0.85 -3.9 337,882 - MMJJ_RS01740 0.85 +1.6 337,884 - MMJJ_RS01740 0.85 +1.8 337,901 + MMJJ_RS01740 0.86 -1.5 337,905 - MMJJ_RS01740 0.87 -1.1 337,906 + MMJJ_RS01740 0.87 -0.5 337,954 + -3.0 338,003 - +3.9 338,003 - +1.8 338,004 + -0.5 338,014 - -1.6 338,015 + +0.2 338,015 + -2.5 338,032 + -1.6 338,046 - -3.6 338,047 + +0.3 338,047 + +2.6 338,058 - -0.8 338,075 - +0.4 338,075 - +0.2 338,076 + -0.3 338,076 + +0.1 338,076 + +1.3 338,082 + -3.4 338,082 + +0.2 338,082 + -1.8 338,093 + +0.4 338,097 - +3.2 338,112 - +2.9 338,113 + +0.2 338,117 + -2.6 338,125 - -1.7 338,126 + +1.2 338,126 + +1.8 338,126 + -3.8 338,127 - -2.8 338,128 + +2.4 338,128 + -3.6 338,141 + -1.1 338,152 + -1.2 338,158 - +1.9 338,159 + -0.3 338,159 + +2.0 338,196 - +2.4 338,205 - -2.5 338,205 - -0.8 338,206 + -0.8 338,206 + -3.1 338,234 - -1.5 338,234 - -1.9 338,235 + -1.1 338,239 + +6.4 338,289 + MMJJ_RS01745 0.12 +0.4 338,298 - MMJJ_RS01745 0.14 -2.4 338,301 + MMJJ_RS01745 0.15 +2.0 338,324 - MMJJ_RS01745 0.21 +0.1 338,324 - MMJJ_RS01745 0.21 +0.3 338,337 + MMJJ_RS01745 0.24 +3.1 338,341 - MMJJ_RS01745 0.25 +2.6 338,341 - MMJJ_RS01745 0.25 +2.7 338,342 + MMJJ_RS01745 0.25 -4.3 338,473 - MMJJ_RS01745 0.57 -0.1 338,482 - MMJJ_RS01745 0.60 +2.3 338,586 + MMJJ_RS01745 0.85 -1.7 338,601 - MMJJ_RS01745 0.89 +1.0 338,620 - -0.1 338,630 - +2.2 338,631 + +0.9 338,666 - -0.1 338,666 - -0.3 338,667 + +1.4 338,667 + -3.0 338,668 - -3.2 338,668 - -2.2 338,668 - +1.2 338,668 - -3.0 338,668 - +1.3 338,669 + +2.6 338,669 + +0.4 338,669 + +2.3 338,669 + -2.1 338,669 + -2.2 338,670 - -0.8 338,671 + -3.4 338,673 + -2.1 338,737 - +0.2 338,740 + +0.5 338,741 - -2.9 338,791 + MMJJ_RS01750 0.16 -0.3 338,792 - MMJJ_RS01750 0.17 -1.9 338,793 + MMJJ_RS01750 0.17 +0.2 338,797 - MMJJ_RS01750 0.19 -0.7 338,869 - MMJJ_RS01750 0.54 +0.2 338,894 + MMJJ_RS01750 0.66 +2.5 338,956 - +0.2 338,962 + -1.4 338,962 + +1.6 338,983 - -3.7 338,992 + -2.6 338,994 + +0.3 338,994 + -3.8 338,999 + +2.5 338,999 + -0.4 338,999 + +2.6 338,999 + +3.5 338,999 + -1.1 338,999 + -1.8 338,999 + +0.9 339,003 - +3.3 339,018 + +0.9 339,046 + +0.9
Or see this region's nucleotide sequence