Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01300 and MMJJ_RS01305 are separated by 12 nucleotides MMJJ_RS01305 and MMJJ_RS01310 are separated by 13 nucleotides MMJJ_RS01310 and MMJJ_RS01315 are separated by 10 nucleotides MMJJ_RS01315 and MMJJ_RS01320 overlap by 1 nucleotides
MMJJ_RS01300: MMJJ_RS01300 - MarR family transcriptional regulator, at 249,295 to 249,831
_RS01300
MMJJ_RS01305: MMJJ_RS01305 - ACT domain-containing protein, at 249,844 to 250,269
_RS01305
MMJJ_RS01310: MMJJ_RS01310 - phenylacetate--CoA ligase, at 250,283 to 251,581
_RS01310
MMJJ_RS01315: MMJJ_RS01315 - indolepyruvate oxidoreductase subunit beta, at 251,592 to 252,158
_RS01315
MMJJ_RS01320: MMJJ_RS01320 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 252,158 to 253,894
_RS01320
Position (kb) 250
251
252 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 249.314 kb on - strand at 249.314 kb on - strand at 249.315 kb on + strand at 249.315 kb on + strand at 249.315 kb on + strand at 249.315 kb on + strand at 249.315 kb on + strand at 249.319 kb on - strand at 249.328 kb on - strand at 249.344 kb on - strand at 249.344 kb on - strand at 249.352 kb on + strand, within MMJJ_RS01300 at 249.352 kb on + strand, within MMJJ_RS01300 at 249.371 kb on - strand, within MMJJ_RS01300 at 249.455 kb on - strand, within MMJJ_RS01300 at 249.455 kb on - strand, within MMJJ_RS01300 at 249.499 kb on - strand, within MMJJ_RS01300 at 249.500 kb on + strand, within MMJJ_RS01300 at 249.502 kb on - strand, within MMJJ_RS01300 at 249.502 kb on - strand, within MMJJ_RS01300 at 249.502 kb on - strand, within MMJJ_RS01300 at 249.503 kb on + strand, within MMJJ_RS01300 at 249.503 kb on + strand, within MMJJ_RS01300 at 249.503 kb on + strand, within MMJJ_RS01300 at 249.503 kb on + strand, within MMJJ_RS01300 at 249.530 kb on - strand, within MMJJ_RS01300 at 249.554 kb on - strand, within MMJJ_RS01300 at 249.555 kb on + strand, within MMJJ_RS01300 at 249.555 kb on + strand, within MMJJ_RS01300 at 249.595 kb on + strand, within MMJJ_RS01300 at 249.622 kb on + strand, within MMJJ_RS01300 at 249.626 kb on - strand, within MMJJ_RS01300 at 249.627 kb on + strand, within MMJJ_RS01300 at 249.643 kb on - strand, within MMJJ_RS01300 at 249.643 kb on - strand, within MMJJ_RS01300 at 249.716 kb on - strand, within MMJJ_RS01300 at 249.727 kb on - strand, within MMJJ_RS01300 at 249.728 kb on + strand, within MMJJ_RS01300 at 249.728 kb on + strand, within MMJJ_RS01300 at 249.732 kb on + strand, within MMJJ_RS01300 at 249.772 kb on + strand, within MMJJ_RS01300 at 249.775 kb on + strand, within MMJJ_RS01300 at 249.856 kb on - strand at 249.857 kb on + strand at 249.857 kb on + strand at 249.857 kb on + strand at 249.857 kb on + strand at 249.857 kb on + strand at 249.858 kb on - strand at 249.859 kb on + strand at 249.871 kb on - strand at 249.896 kb on + strand, within MMJJ_RS01305 at 249.898 kb on - strand, within MMJJ_RS01305 at 249.898 kb on - strand, within MMJJ_RS01305 at 249.898 kb on - strand, within MMJJ_RS01305 at 249.899 kb on + strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.970 kb on - strand, within MMJJ_RS01305 at 249.971 kb on + strand, within MMJJ_RS01305 at 249.971 kb on + strand, within MMJJ_RS01305 at 249.971 kb on + strand, within MMJJ_RS01305 at 249.974 kb on - strand, within MMJJ_RS01305 at 249.974 kb on - strand, within MMJJ_RS01305 at 249.974 kb on - strand, within MMJJ_RS01305 at 249.975 kb on + strand, within MMJJ_RS01305 at 249.975 kb on + strand, within MMJJ_RS01305 at 249.975 kb on + strand, within MMJJ_RS01305 at 249.975 kb on + strand, within MMJJ_RS01305 at 250.003 kb on - strand, within MMJJ_RS01305 at 250.005 kb on - strand, within MMJJ_RS01305 at 250.006 kb on + strand, within MMJJ_RS01305 at 250.006 kb on + strand, within MMJJ_RS01305 at 250.008 kb on - strand, within MMJJ_RS01305 at 250.009 kb on + strand, within MMJJ_RS01305 at 250.009 kb on + strand, within MMJJ_RS01305 at 250.009 kb on + strand, within MMJJ_RS01305 at 250.009 kb on + strand, within MMJJ_RS01305 at 250.045 kb on + strand, within MMJJ_RS01305 at 250.048 kb on - strand, within MMJJ_RS01305 at 250.048 kb on - strand, within MMJJ_RS01305 at 250.048 kb on - strand, within MMJJ_RS01305 at 250.048 kb on - strand, within MMJJ_RS01305 at 250.048 kb on - strand, within MMJJ_RS01305 at 250.048 kb on - strand, within MMJJ_RS01305 at 250.049 kb on + strand, within MMJJ_RS01305 at 250.049 kb on + strand, within MMJJ_RS01305 at 250.049 kb on + strand, within MMJJ_RS01305 at 250.049 kb on + strand, within MMJJ_RS01305 at 250.049 kb on + strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.050 kb on - strand, within MMJJ_RS01305 at 250.051 kb on + strand, within MMJJ_RS01305 at 250.051 kb on + strand, within MMJJ_RS01305 at 250.051 kb on + strand, within MMJJ_RS01305 at 250.051 kb on + strand, within MMJJ_RS01305 at 250.054 kb on - strand, within MMJJ_RS01305 at 250.055 kb on + strand, within MMJJ_RS01305 at 250.084 kb on + strand, within MMJJ_RS01305 at 250.094 kb on + strand, within MMJJ_RS01305 at 250.210 kb on - strand, within MMJJ_RS01305 at 250.246 kb on - strand at 250.284 kb on - strand at 250.286 kb on - strand at 250.370 kb on - strand at 250.371 kb on + strand at 250.393 kb on - strand at 250.412 kb on - strand at 250.412 kb on - strand at 250.412 kb on - strand at 250.413 kb on + strand, within MMJJ_RS01310 at 250.499 kb on + strand, within MMJJ_RS01310 at 250.508 kb on - strand, within MMJJ_RS01310 at 250.512 kb on + strand, within MMJJ_RS01310 at 250.572 kb on + strand, within MMJJ_RS01310 at 250.587 kb on + strand, within MMJJ_RS01310 at 250.588 kb on - strand, within MMJJ_RS01310 at 250.588 kb on - strand, within MMJJ_RS01310 at 250.588 kb on - strand, within MMJJ_RS01310 at 250.593 kb on + strand, within MMJJ_RS01310 at 250.668 kb on + strand, within MMJJ_RS01310 at 250.697 kb on - strand, within MMJJ_RS01310 at 250.729 kb on - strand, within MMJJ_RS01310 at 250.729 kb on - strand, within MMJJ_RS01310 at 250.733 kb on - strand, within MMJJ_RS01310 at 250.734 kb on + strand, within MMJJ_RS01310 at 250.775 kb on + strand, within MMJJ_RS01310 at 250.787 kb on + strand, within MMJJ_RS01310 at 250.865 kb on - strand, within MMJJ_RS01310 at 250.865 kb on - strand, within MMJJ_RS01310 at 250.881 kb on - strand, within MMJJ_RS01310 at 250.882 kb on + strand, within MMJJ_RS01310 at 250.883 kb on - strand, within MMJJ_RS01310 at 250.883 kb on - strand, within MMJJ_RS01310 at 250.884 kb on + strand, within MMJJ_RS01310 at 250.884 kb on + strand, within MMJJ_RS01310 at 250.884 kb on + strand, within MMJJ_RS01310 at 250.886 kb on + strand, within MMJJ_RS01310 at 250.886 kb on + strand, within MMJJ_RS01310 at 251.022 kb on - strand, within MMJJ_RS01310 at 251.023 kb on + strand, within MMJJ_RS01310 at 251.023 kb on + strand, within MMJJ_RS01310 at 251.031 kb on - strand, within MMJJ_RS01310 at 251.118 kb on + strand, within MMJJ_RS01310 at 251.145 kb on - strand, within MMJJ_RS01310 at 251.168 kb on + strand, within MMJJ_RS01310 at 251.184 kb on - strand, within MMJJ_RS01310 at 251.229 kb on + strand, within MMJJ_RS01310 at 251.243 kb on - strand, within MMJJ_RS01310 at 251.244 kb on + strand, within MMJJ_RS01310 at 251.282 kb on - strand, within MMJJ_RS01310 at 251.379 kb on + strand, within MMJJ_RS01310 at 251.396 kb on + strand, within MMJJ_RS01310 at 251.396 kb on + strand, within MMJJ_RS01310 at 251.452 kb on - strand at 251.453 kb on + strand at 251.453 kb on + strand at 251.500 kb on - strand at 251.506 kb on - strand at 251.564 kb on + strand at 251.564 kb on + strand at 251.665 kb on + strand, within MMJJ_RS01315 at 251.674 kb on + strand, within MMJJ_RS01315 at 251.742 kb on - strand, within MMJJ_RS01315 at 251.742 kb on - strand, within MMJJ_RS01315 at 251.769 kb on - strand, within MMJJ_RS01315 at 251.778 kb on - strand, within MMJJ_RS01315 at 251.787 kb on + strand, within MMJJ_RS01315 at 251.908 kb on + strand, within MMJJ_RS01315 at 251.920 kb on - strand, within MMJJ_RS01315 at 251.920 kb on - strand, within MMJJ_RS01315 at 251.920 kb on - strand, within MMJJ_RS01315 at 251.921 kb on + strand, within MMJJ_RS01315 at 252.008 kb on - strand, within MMJJ_RS01315 at 252.013 kb on - strand, within MMJJ_RS01315 at 252.013 kb on - strand, within MMJJ_RS01315 at 252.013 kb on - strand, within MMJJ_RS01315 at 252.014 kb on + strand, within MMJJ_RS01315 at 252.014 kb on + strand, within MMJJ_RS01315 at 252.164 kb on - strand at 252.176 kb on - strand at 252.176 kb on - strand at 252.176 kb on - strand at 252.290 kb on - strand at 252.290 kb on - strand at 252.291 kb on + strand at 252.295 kb on - strand at 252.295 kb on - strand at 252.326 kb on + strand at 252.362 kb on - strand, within MMJJ_RS01320 at 252.363 kb on + strand, within MMJJ_RS01320 at 252.441 kb on + strand, within MMJJ_RS01320 at 252.470 kb on + strand, within MMJJ_RS01320 at 252.473 kb on - strand, within MMJJ_RS01320 at 252.474 kb on + strand, within MMJJ_RS01320 at 252.577 kb on - strand, within MMJJ_RS01320 at 252.577 kb on - strand, within MMJJ_RS01320 at 252.578 kb on + strand, within MMJJ_RS01320
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM remove 249,314 - +0.5 249,314 - -1.1 249,315 + +2.9 249,315 + -1.8 249,315 + -2.3 249,315 + +0.2 249,315 + +2.2 249,319 - -3.1 249,328 - +2.9 249,344 - +0.8 249,344 - +2.6 249,352 + MMJJ_RS01300 0.11 +1.6 249,352 + MMJJ_RS01300 0.11 -1.8 249,371 - MMJJ_RS01300 0.14 +2.6 249,455 - MMJJ_RS01300 0.30 +3.0 249,455 - MMJJ_RS01300 0.30 -0.5 249,499 - MMJJ_RS01300 0.38 +0.4 249,500 + MMJJ_RS01300 0.38 +0.9 249,502 - MMJJ_RS01300 0.39 -2.1 249,502 - MMJJ_RS01300 0.39 +0.9 249,502 - MMJJ_RS01300 0.39 -2.0 249,503 + MMJJ_RS01300 0.39 -1.1 249,503 + MMJJ_RS01300 0.39 -2.9 249,503 + MMJJ_RS01300 0.39 -1.7 249,503 + MMJJ_RS01300 0.39 -2.7 249,530 - MMJJ_RS01300 0.44 -0.6 249,554 - MMJJ_RS01300 0.48 +1.6 249,555 + MMJJ_RS01300 0.48 -1.9 249,555 + MMJJ_RS01300 0.48 +1.2 249,595 + MMJJ_RS01300 0.56 +2.4 249,622 + MMJJ_RS01300 0.61 +1.1 249,626 - MMJJ_RS01300 0.62 -1.0 249,627 + MMJJ_RS01300 0.62 +0.5 249,643 - MMJJ_RS01300 0.65 +0.4 249,643 - MMJJ_RS01300 0.65 +1.5 249,716 - MMJJ_RS01300 0.78 +4.7 249,727 - MMJJ_RS01300 0.80 +1.7 249,728 + MMJJ_RS01300 0.81 +0.7 249,728 + MMJJ_RS01300 0.81 -1.7 249,732 + MMJJ_RS01300 0.81 +2.2 249,772 + MMJJ_RS01300 0.89 -1.3 249,775 + MMJJ_RS01300 0.89 -2.2 249,856 - +0.1 249,857 + -2.7 249,857 + -2.1 249,857 + -1.8 249,857 + +0.6 249,857 + +3.3 249,858 - -1.9 249,859 + -0.1 249,871 - -0.9 249,896 + MMJJ_RS01305 0.12 -2.0 249,898 - MMJJ_RS01305 0.13 +1.7 249,898 - MMJJ_RS01305 0.13 -1.9 249,898 - MMJJ_RS01305 0.13 +2.2 249,899 + MMJJ_RS01305 0.13 -2.9 249,970 - MMJJ_RS01305 0.30 +1.4 249,970 - MMJJ_RS01305 0.30 -2.3 249,970 - MMJJ_RS01305 0.30 +1.8 249,970 - MMJJ_RS01305 0.30 -1.2 249,970 - MMJJ_RS01305 0.30 -2.6 249,970 - MMJJ_RS01305 0.30 +0.1 249,970 - MMJJ_RS01305 0.30 +0.3 249,971 + MMJJ_RS01305 0.30 -2.3 249,971 + MMJJ_RS01305 0.30 +2.3 249,971 + MMJJ_RS01305 0.30 -1.2 249,974 - MMJJ_RS01305 0.31 -0.1 249,974 - MMJJ_RS01305 0.31 +1.8 249,974 - MMJJ_RS01305 0.31 -2.5 249,975 + MMJJ_RS01305 0.31 +1.0 249,975 + MMJJ_RS01305 0.31 -0.2 249,975 + MMJJ_RS01305 0.31 +2.4 249,975 + MMJJ_RS01305 0.31 -2.9 250,003 - MMJJ_RS01305 0.37 +2.6 250,005 - MMJJ_RS01305 0.38 +2.5 250,006 + MMJJ_RS01305 0.38 -2.7 250,006 + MMJJ_RS01305 0.38 +0.1 250,008 - MMJJ_RS01305 0.38 -0.2 250,009 + MMJJ_RS01305 0.39 -2.3 250,009 + MMJJ_RS01305 0.39 +0.6 250,009 + MMJJ_RS01305 0.39 +2.7 250,009 + MMJJ_RS01305 0.39 +0.2 250,045 + MMJJ_RS01305 0.47 +0.9 250,048 - MMJJ_RS01305 0.48 +0.9 250,048 - MMJJ_RS01305 0.48 -0.8 250,048 - MMJJ_RS01305 0.48 +0.3 250,048 - MMJJ_RS01305 0.48 -1.7 250,048 - MMJJ_RS01305 0.48 -0.4 250,048 - MMJJ_RS01305 0.48 +2.0 250,049 + MMJJ_RS01305 0.48 -2.1 250,049 + MMJJ_RS01305 0.48 -2.3 250,049 + MMJJ_RS01305 0.48 +1.7 250,049 + MMJJ_RS01305 0.48 -4.2 250,049 + MMJJ_RS01305 0.48 -0.3 250,050 - MMJJ_RS01305 0.48 +0.5 250,050 - MMJJ_RS01305 0.48 +3.2 250,050 - MMJJ_RS01305 0.48 -1.0 250,050 - MMJJ_RS01305 0.48 +0.1 250,050 - MMJJ_RS01305 0.48 -1.7 250,050 - MMJJ_RS01305 0.48 +1.1 250,050 - MMJJ_RS01305 0.48 +1.9 250,050 - MMJJ_RS01305 0.48 +2.6 250,050 - MMJJ_RS01305 0.48 -3.1 250,051 + MMJJ_RS01305 0.49 -3.3 250,051 + MMJJ_RS01305 0.49 -0.4 250,051 + MMJJ_RS01305 0.49 -3.2 250,051 + MMJJ_RS01305 0.49 -0.6 250,054 - MMJJ_RS01305 0.49 -2.6 250,055 + MMJJ_RS01305 0.50 -3.5 250,084 + MMJJ_RS01305 0.56 +1.2 250,094 + MMJJ_RS01305 0.59 -0.1 250,210 - MMJJ_RS01305 0.86 -3.1 250,246 - -2.6 250,284 - +0.2 250,286 - +3.3 250,370 - +2.2 250,371 + +2.7 250,393 - -3.8 250,412 - +2.5 250,412 - -1.0 250,412 - +2.1 250,413 + MMJJ_RS01310 0.10 -4.9 250,499 + MMJJ_RS01310 0.17 +2.9 250,508 - MMJJ_RS01310 0.17 +2.0 250,512 + MMJJ_RS01310 0.18 +2.9 250,572 + MMJJ_RS01310 0.22 -1.8 250,587 + MMJJ_RS01310 0.23 -0.1 250,588 - MMJJ_RS01310 0.23 +1.6 250,588 - MMJJ_RS01310 0.23 -4.5 250,588 - MMJJ_RS01310 0.23 -2.4 250,593 + MMJJ_RS01310 0.24 +2.5 250,668 + MMJJ_RS01310 0.30 +3.6 250,697 - MMJJ_RS01310 0.32 -3.3 250,729 - MMJJ_RS01310 0.34 -2.2 250,729 - MMJJ_RS01310 0.34 +2.2 250,733 - MMJJ_RS01310 0.35 -0.7 250,734 + MMJJ_RS01310 0.35 +2.3 250,775 + MMJJ_RS01310 0.38 -3.4 250,787 + MMJJ_RS01310 0.39 -2.5 250,865 - MMJJ_RS01310 0.45 -2.7 250,865 - MMJJ_RS01310 0.45 +2.5 250,881 - MMJJ_RS01310 0.46 +1.4 250,882 + MMJJ_RS01310 0.46 -0.6 250,883 - MMJJ_RS01310 0.46 -2.2 250,883 - MMJJ_RS01310 0.46 -2.9 250,884 + MMJJ_RS01310 0.46 -1.4 250,884 + MMJJ_RS01310 0.46 -1.9 250,884 + MMJJ_RS01310 0.46 +1.9 250,886 + MMJJ_RS01310 0.46 -0.8 250,886 + MMJJ_RS01310 0.46 -1.5 251,022 - MMJJ_RS01310 0.57 +1.4 251,023 + MMJJ_RS01310 0.57 -0.5 251,023 + MMJJ_RS01310 0.57 +2.4 251,031 - MMJJ_RS01310 0.58 +0.6 251,118 + MMJJ_RS01310 0.64 +2.2 251,145 - MMJJ_RS01310 0.66 +1.3 251,168 + MMJJ_RS01310 0.68 +0.6 251,184 - MMJJ_RS01310 0.69 +0.9 251,229 + MMJJ_RS01310 0.73 +2.5 251,243 - MMJJ_RS01310 0.74 +0.6 251,244 + MMJJ_RS01310 0.74 -1.6 251,282 - MMJJ_RS01310 0.77 -1.8 251,379 + MMJJ_RS01310 0.84 +1.1 251,396 + MMJJ_RS01310 0.86 +2.0 251,396 + MMJJ_RS01310 0.86 +0.8 251,452 - -0.2 251,453 + -0.7 251,453 + +3.6 251,500 - -3.0 251,506 - -1.8 251,564 + -0.2 251,564 + +0.2 251,665 + MMJJ_RS01315 0.13 -2.2 251,674 + MMJJ_RS01315 0.14 +0.3 251,742 - MMJJ_RS01315 0.26 -0.9 251,742 - MMJJ_RS01315 0.26 +0.1 251,769 - MMJJ_RS01315 0.31 +1.1 251,778 - MMJJ_RS01315 0.33 -0.2 251,787 + MMJJ_RS01315 0.34 +1.5 251,908 + MMJJ_RS01315 0.56 +1.2 251,920 - MMJJ_RS01315 0.58 +0.1 251,920 - MMJJ_RS01315 0.58 -0.6 251,920 - MMJJ_RS01315 0.58 -3.3 251,921 + MMJJ_RS01315 0.58 +1.4 252,008 - MMJJ_RS01315 0.73 -3.3 252,013 - MMJJ_RS01315 0.74 -3.2 252,013 - MMJJ_RS01315 0.74 +3.6 252,013 - MMJJ_RS01315 0.74 -4.3 252,014 + MMJJ_RS01315 0.74 -1.2 252,014 + MMJJ_RS01315 0.74 -0.6 252,164 - +1.5 252,176 - +0.5 252,176 - -1.2 252,176 - -1.8 252,290 - -0.4 252,290 - +0.7 252,291 + +2.1 252,295 - +1.6 252,295 - -1.6 252,326 + +0.2 252,362 - MMJJ_RS01320 0.12 +1.1 252,363 + MMJJ_RS01320 0.12 -0.4 252,441 + MMJJ_RS01320 0.16 +3.0 252,470 + MMJJ_RS01320 0.18 +0.5 252,473 - MMJJ_RS01320 0.18 +1.5 252,474 + MMJJ_RS01320 0.18 -0.2 252,577 - MMJJ_RS01320 0.24 -1.0 252,577 - MMJJ_RS01320 0.24 +2.3 252,578 + MMJJ_RS01320 0.24 -1.3
Or see this region's nucleotide sequence