Experiment: McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05825 and MMJJ_RS05830 are separated by 158 nucleotides MMJJ_RS05830 and MMJJ_RS05835 are separated by 46 nucleotides MMJJ_RS05835 and MMJJ_RS05840 are separated by 122 nucleotides MMJJ_RS05840 and MMJJ_RS05845 are separated by 116 nucleotides MMJJ_RS05845 and MMJJ_RS05850 are separated by 38 nucleotides MMJJ_RS05850 and MMJJ_RS05855 are separated by 14 nucleotides MMJJ_RS05855 and MMJJ_RS05860 are separated by 13 nucleotides MMJJ_RS05860 and MMJJ_RS05865 are separated by 29 nucleotides MMJJ_RS05865 and MMJJ_RS05870 are separated by 114 nucleotides
MMJJ_RS05825: MMJJ_RS05825 - aspartate carbamoyltransferase, at 1,084,513 to 1,085,421
_RS05825
MMJJ_RS05830: MMJJ_RS05830 - 30S ribosomal protein S8e, at 1,085,580 to 1,085,966
_RS05830
MMJJ_RS05835: MMJJ_RS05835 - bis-aminopropyl spermidine synthase family protein, at 1,086,013 to 1,087,185
_RS05835
MMJJ_RS05840: MMJJ_RS05840 - tRNA-Phe, at 1,087,308 to 1,087,381
_RS05840
MMJJ_RS05845: MMJJ_RS05845 - tRNA-Asn, at 1,087,498 to 1,087,574
_RS05845
MMJJ_RS05850: MMJJ_RS05850 - tRNA-Ile, at 1,087,613 to 1,087,687
_RS05850
MMJJ_RS05855: MMJJ_RS05855 - tRNA-Glu, at 1,087,702 to 1,087,776
_RS05855
MMJJ_RS05860: MMJJ_RS05860 - tRNA-Leu, at 1,087,790 to 1,087,874
_RS05860
MMJJ_RS05865: MMJJ_RS05865 - tRNA-His, at 1,087,904 to 1,087,978
_RS05865
MMJJ_RS05870: MMJJ_RS05870 - pyridoxal 5'-phosphate synthase glutaminase subunit PdxT, at 1,088,093 to 1,088,656
_RS05870
Position (kb)
1086
1087
1088 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1085.216 kb on + strand, within MMJJ_RS05825 at 1085.225 kb on - strand, within MMJJ_RS05825 at 1085.235 kb on + strand, within MMJJ_RS05825 at 1085.391 kb on + strand at 1085.423 kb on - strand at 1085.428 kb on - strand at 1085.432 kb on + strand at 1085.437 kb on - strand at 1085.438 kb on + strand at 1085.441 kb on - strand at 1085.441 kb on - strand at 1085.442 kb on + strand at 1085.447 kb on + strand at 1085.495 kb on + strand at 1085.525 kb on + strand at 1085.953 kb on - strand at 1085.964 kb on - strand at 1085.965 kb on + strand at 1086.025 kb on - strand at 1086.095 kb on + strand at 1086.140 kb on - strand, within MMJJ_RS05835 at 1086.141 kb on + strand, within MMJJ_RS05835 at 1086.144 kb on + strand, within MMJJ_RS05835 at 1086.149 kb on + strand, within MMJJ_RS05835 at 1086.189 kb on - strand, within MMJJ_RS05835 at 1086.197 kb on + strand, within MMJJ_RS05835 at 1086.249 kb on - strand, within MMJJ_RS05835 at 1086.250 kb on + strand, within MMJJ_RS05835 at 1086.266 kb on - strand, within MMJJ_RS05835 at 1086.274 kb on + strand, within MMJJ_RS05835 at 1086.299 kb on + strand, within MMJJ_RS05835 at 1086.338 kb on - strand, within MMJJ_RS05835 at 1086.371 kb on - strand, within MMJJ_RS05835 at 1086.372 kb on + strand, within MMJJ_RS05835 at 1086.372 kb on + strand, within MMJJ_RS05835 at 1086.376 kb on - strand, within MMJJ_RS05835 at 1086.433 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.438 kb on - strand, within MMJJ_RS05835 at 1086.439 kb on + strand, within MMJJ_RS05835 at 1086.439 kb on + strand, within MMJJ_RS05835 at 1086.439 kb on + strand, within MMJJ_RS05835 at 1086.439 kb on + strand, within MMJJ_RS05835 at 1086.439 kb on + strand, within MMJJ_RS05835 at 1086.439 kb on + strand, within MMJJ_RS05835 at 1086.440 kb on - strand, within MMJJ_RS05835 at 1086.457 kb on - strand, within MMJJ_RS05835 at 1086.458 kb on + strand, within MMJJ_RS05835 at 1086.458 kb on + strand, within MMJJ_RS05835 at 1086.463 kb on + strand, within MMJJ_RS05835 at 1086.471 kb on - strand, within MMJJ_RS05835 at 1086.501 kb on - strand, within MMJJ_RS05835 at 1086.501 kb on - strand, within MMJJ_RS05835 at 1086.514 kb on - strand, within MMJJ_RS05835 at 1086.522 kb on - strand, within MMJJ_RS05835 at 1086.522 kb on - strand, within MMJJ_RS05835 at 1086.523 kb on + strand, within MMJJ_RS05835 at 1086.523 kb on + strand, within MMJJ_RS05835 at 1086.586 kb on - strand, within MMJJ_RS05835 at 1086.587 kb on + strand, within MMJJ_RS05835 at 1086.658 kb on - strand, within MMJJ_RS05835 at 1086.659 kb on + strand, within MMJJ_RS05835 at 1086.757 kb on - strand, within MMJJ_RS05835 at 1086.758 kb on + strand, within MMJJ_RS05835 at 1086.775 kb on + strand, within MMJJ_RS05835 at 1086.779 kb on - strand at 1086.828 kb on - strand, within MMJJ_RS05835 at 1086.829 kb on + strand, within MMJJ_RS05835 at 1086.829 kb on + strand, within MMJJ_RS05835 at 1086.844 kb on - strand, within MMJJ_RS05835 at 1086.845 kb on + strand, within MMJJ_RS05835 at 1086.867 kb on - strand, within MMJJ_RS05835 at 1086.867 kb on - strand, within MMJJ_RS05835 at 1086.867 kb on - strand, within MMJJ_RS05835 at 1086.868 kb on + strand, within MMJJ_RS05835 at 1086.873 kb on - strand, within MMJJ_RS05835 at 1086.873 kb on - strand, within MMJJ_RS05835 at 1086.873 kb on - strand, within MMJJ_RS05835 at 1086.874 kb on + strand, within MMJJ_RS05835 at 1086.874 kb on + strand, within MMJJ_RS05835 at 1086.972 kb on - strand, within MMJJ_RS05835 at 1086.972 kb on - strand, within MMJJ_RS05835 at 1086.973 kb on + strand, within MMJJ_RS05835 at 1086.978 kb on - strand, within MMJJ_RS05835 at 1086.982 kb on - strand, within MMJJ_RS05835 at 1086.982 kb on - strand, within MMJJ_RS05835 at 1087.003 kb on - strand, within MMJJ_RS05835 at 1087.003 kb on - strand, within MMJJ_RS05835 at 1087.004 kb on + strand, within MMJJ_RS05835 at 1087.007 kb on - strand, within MMJJ_RS05835 at 1087.007 kb on - strand, within MMJJ_RS05835 at 1087.008 kb on + strand, within MMJJ_RS05835 at 1087.047 kb on + strand, within MMJJ_RS05835 at 1087.048 kb on - strand, within MMJJ_RS05835 at 1087.049 kb on + strand, within MMJJ_RS05835 at 1087.049 kb on + strand, within MMJJ_RS05835 at 1087.049 kb on + strand, within MMJJ_RS05835 at 1087.058 kb on + strand, within MMJJ_RS05835 at 1087.062 kb on - strand, within MMJJ_RS05835 at 1087.199 kb on - strand at 1087.199 kb on - strand at 1087.199 kb on - strand at 1087.201 kb on - strand at 1087.201 kb on - strand at 1087.202 kb on + strand at 1087.214 kb on - strand at 1087.217 kb on - strand at 1087.217 kb on - strand at 1087.220 kb on - strand at 1087.220 kb on - strand at 1087.220 kb on - strand at 1087.220 kb on - strand at 1087.225 kb on - strand at 1087.226 kb on + strand at 1087.226 kb on + strand at 1087.226 kb on + strand at 1087.231 kb on - strand at 1087.238 kb on + strand at 1087.242 kb on - strand at 1087.243 kb on + strand at 1087.243 kb on + strand at 1087.253 kb on + strand at 1087.253 kb on + strand at 1088.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM remove 1,085,216 + MMJJ_RS05825 0.77 -0.9 1,085,225 - MMJJ_RS05825 0.78 +1.0 1,085,235 + MMJJ_RS05825 0.79 -1.9 1,085,391 + +2.0 1,085,423 - +0.1 1,085,428 - -1.1 1,085,432 + -2.2 1,085,437 - +1.4 1,085,438 + -1.2 1,085,441 - +1.5 1,085,441 - -1.5 1,085,442 + +0.3 1,085,447 + +2.5 1,085,495 + +1.6 1,085,525 + +0.6 1,085,953 - -0.2 1,085,964 - +0.3 1,085,965 + -1.4 1,086,025 - -2.4 1,086,095 + -1.0 1,086,140 - MMJJ_RS05835 0.11 +2.5 1,086,141 + MMJJ_RS05835 0.11 -1.9 1,086,144 + MMJJ_RS05835 0.11 -1.7 1,086,149 + MMJJ_RS05835 0.12 +1.5 1,086,189 - MMJJ_RS05835 0.15 +1.7 1,086,197 + MMJJ_RS05835 0.16 +1.1 1,086,249 - MMJJ_RS05835 0.20 +0.1 1,086,250 + MMJJ_RS05835 0.20 +1.9 1,086,266 - MMJJ_RS05835 0.22 +1.8 1,086,274 + MMJJ_RS05835 0.22 +1.5 1,086,299 + MMJJ_RS05835 0.24 +2.6 1,086,338 - MMJJ_RS05835 0.28 +0.5 1,086,371 - MMJJ_RS05835 0.31 +2.3 1,086,372 + MMJJ_RS05835 0.31 +0.9 1,086,372 + MMJJ_RS05835 0.31 -2.1 1,086,376 - MMJJ_RS05835 0.31 +0.1 1,086,433 - MMJJ_RS05835 0.36 -0.2 1,086,438 - MMJJ_RS05835 0.36 +2.0 1,086,438 - MMJJ_RS05835 0.36 +0.9 1,086,438 - MMJJ_RS05835 0.36 -0.2 1,086,438 - MMJJ_RS05835 0.36 +0.6 1,086,438 - MMJJ_RS05835 0.36 -1.2 1,086,438 - MMJJ_RS05835 0.36 -0.3 1,086,438 - MMJJ_RS05835 0.36 -2.0 1,086,439 + MMJJ_RS05835 0.36 +1.1 1,086,439 + MMJJ_RS05835 0.36 +1.1 1,086,439 + MMJJ_RS05835 0.36 +0.9 1,086,439 + MMJJ_RS05835 0.36 -4.1 1,086,439 + MMJJ_RS05835 0.36 +2.2 1,086,439 + MMJJ_RS05835 0.36 -3.1 1,086,440 - MMJJ_RS05835 0.36 +0.9 1,086,457 - MMJJ_RS05835 0.38 -0.5 1,086,458 + MMJJ_RS05835 0.38 +0.9 1,086,458 + MMJJ_RS05835 0.38 -1.7 1,086,463 + MMJJ_RS05835 0.38 +0.8 1,086,471 - MMJJ_RS05835 0.39 -2.5 1,086,501 - MMJJ_RS05835 0.42 +0.0 1,086,501 - MMJJ_RS05835 0.42 -0.6 1,086,514 - MMJJ_RS05835 0.43 +0.2 1,086,522 - MMJJ_RS05835 0.43 +0.5 1,086,522 - MMJJ_RS05835 0.43 -2.1 1,086,523 + MMJJ_RS05835 0.43 +1.0 1,086,523 + MMJJ_RS05835 0.43 -0.9 1,086,586 - MMJJ_RS05835 0.49 -0.6 1,086,587 + MMJJ_RS05835 0.49 +0.3 1,086,658 - MMJJ_RS05835 0.55 +2.3 1,086,659 + MMJJ_RS05835 0.55 -1.8 1,086,757 - MMJJ_RS05835 0.63 +0.3 1,086,758 + MMJJ_RS05835 0.64 +0.7 1,086,775 + MMJJ_RS05835 0.65 +1.7 1,086,779 - +1.6 1,086,828 - MMJJ_RS05835 0.69 +2.0 1,086,829 + MMJJ_RS05835 0.70 +2.1 1,086,829 + MMJJ_RS05835 0.70 -3.7 1,086,844 - MMJJ_RS05835 0.71 +1.1 1,086,845 + MMJJ_RS05835 0.71 +1.5 1,086,867 - MMJJ_RS05835 0.73 +0.3 1,086,867 - MMJJ_RS05835 0.73 -4.6 1,086,867 - MMJJ_RS05835 0.73 +0.4 1,086,868 + MMJJ_RS05835 0.73 +3.2 1,086,873 - MMJJ_RS05835 0.73 -1.6 1,086,873 - MMJJ_RS05835 0.73 -1.8 1,086,873 - MMJJ_RS05835 0.73 +0.5 1,086,874 + MMJJ_RS05835 0.73 -2.8 1,086,874 + MMJJ_RS05835 0.73 +0.4 1,086,972 - MMJJ_RS05835 0.82 -2.1 1,086,972 - MMJJ_RS05835 0.82 -0.6 1,086,973 + MMJJ_RS05835 0.82 +0.1 1,086,978 - MMJJ_RS05835 0.82 +1.6 1,086,982 - MMJJ_RS05835 0.83 +1.3 1,086,982 - MMJJ_RS05835 0.83 -3.4 1,087,003 - MMJJ_RS05835 0.84 +1.0 1,087,003 - MMJJ_RS05835 0.84 +2.5 1,087,004 + MMJJ_RS05835 0.84 +0.9 1,087,007 - MMJJ_RS05835 0.85 -1.6 1,087,007 - MMJJ_RS05835 0.85 -0.9 1,087,008 + MMJJ_RS05835 0.85 +0.6 1,087,047 + MMJJ_RS05835 0.88 +0.3 1,087,048 - MMJJ_RS05835 0.88 -1.3 1,087,049 + MMJJ_RS05835 0.88 +2.1 1,087,049 + MMJJ_RS05835 0.88 +2.9 1,087,049 + MMJJ_RS05835 0.88 +2.4 1,087,058 + MMJJ_RS05835 0.89 +1.0 1,087,062 - MMJJ_RS05835 0.89 +1.0 1,087,199 - +1.6 1,087,199 - +1.2 1,087,199 - +3.1 1,087,201 - -1.6 1,087,201 - -1.1 1,087,202 + +2.1 1,087,214 - +0.7 1,087,217 - -1.6 1,087,217 - +3.2 1,087,220 - +1.2 1,087,220 - +3.8 1,087,220 - +0.2 1,087,220 - +0.4 1,087,225 - -1.9 1,087,226 + +0.1 1,087,226 + -0.7 1,087,226 + +2.8 1,087,231 - -0.6 1,087,238 + +1.6 1,087,242 - -1.0 1,087,243 + -1.6 1,087,243 + -2.5 1,087,253 + -0.3 1,087,253 + -0.5 1,088,052 + +1.9
Or see this region's nucleotide sequence