Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00830 and MPMX20_00831 are separated by 59 nucleotides MPMX20_00831 and MPMX20_00832 are separated by 176 nucleotides MPMX20_00832 and MPMX20_00833 overlap by 11 nucleotides
MPMX20_00830: MPMX20_00830 - Glutamyl-Q tRNA(Asp) synthetase, at 872,181 to 873,071
_00830
MPMX20_00831: MPMX20_00831 - RNA polymerase-binding transcription factor DksA, at 873,131 to 873,586
_00831
MPMX20_00832: MPMX20_00832 - Sugar fermentation stimulation protein A, at 873,763 to 874,467
_00832
MPMX20_00833: MPMX20_00833 - RNA 2',3'-cyclic phosphodiesterase, at 874,457 to 875,011
_00833
Position (kb) 873
874 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 872.138 kb on - strand at 872.187 kb on - strand at 872.328 kb on + strand, within MPMX20_00830 at 872.336 kb on - strand, within MPMX20_00830 at 872.356 kb on + strand, within MPMX20_00830 at 872.395 kb on - strand, within MPMX20_00830 at 872.402 kb on - strand, within MPMX20_00830 at 872.492 kb on + strand, within MPMX20_00830 at 872.492 kb on + strand, within MPMX20_00830 at 872.492 kb on + strand, within MPMX20_00830 at 872.500 kb on - strand, within MPMX20_00830 at 872.500 kb on - strand, within MPMX20_00830 at 872.515 kb on - strand, within MPMX20_00830 at 872.560 kb on - strand, within MPMX20_00830 at 872.690 kb on + strand, within MPMX20_00830 at 872.690 kb on + strand, within MPMX20_00830 at 872.693 kb on - strand, within MPMX20_00830 at 872.693 kb on - strand, within MPMX20_00830 at 872.698 kb on - strand, within MPMX20_00830 at 872.698 kb on - strand, within MPMX20_00830 at 872.698 kb on - strand, within MPMX20_00830 at 872.734 kb on - strand, within MPMX20_00830 at 872.760 kb on + strand, within MPMX20_00830 at 872.769 kb on - strand, within MPMX20_00830 at 872.895 kb on + strand, within MPMX20_00830 at 872.900 kb on + strand, within MPMX20_00830 at 872.974 kb on - strand, within MPMX20_00830 at 873.047 kb on + strand at 873.069 kb on + strand at 873.103 kb on + strand at 873.111 kb on - strand at 873.111 kb on - strand at 873.111 kb on - strand at 873.113 kb on + strand at 873.127 kb on + strand at 873.187 kb on + strand, within MPMX20_00831 at 873.188 kb on - strand, within MPMX20_00831 at 873.195 kb on - strand, within MPMX20_00831 at 873.329 kb on + strand, within MPMX20_00831 at 873.339 kb on - strand, within MPMX20_00831 at 873.364 kb on + strand, within MPMX20_00831 at 873.364 kb on + strand, within MPMX20_00831 at 873.470 kb on - strand, within MPMX20_00831 at 873.470 kb on - strand, within MPMX20_00831 at 873.484 kb on - strand, within MPMX20_00831 at 873.486 kb on - strand, within MPMX20_00831 at 873.596 kb on + strand at 873.608 kb on + strand at 873.608 kb on + strand at 873.616 kb on - strand at 873.616 kb on - strand at 873.617 kb on + strand at 873.650 kb on - strand at 873.650 kb on - strand at 873.683 kb on + strand at 873.691 kb on + strand at 873.691 kb on - strand at 873.691 kb on - strand at 873.693 kb on + strand at 873.693 kb on + strand at 873.693 kb on + strand at 873.737 kb on - strand at 873.762 kb on - strand at 873.802 kb on - strand at 873.826 kb on + strand at 873.827 kb on + strand at 873.834 kb on + strand, within MPMX20_00832 at 873.842 kb on + strand, within MPMX20_00832 at 873.930 kb on - strand, within MPMX20_00832 at 873.937 kb on + strand, within MPMX20_00832 at 873.937 kb on + strand, within MPMX20_00832 at 873.937 kb on + strand, within MPMX20_00832 at 873.945 kb on - strand, within MPMX20_00832 at 873.945 kb on - strand, within MPMX20_00832 at 873.945 kb on - strand, within MPMX20_00832 at 873.945 kb on - strand, within MPMX20_00832 at 873.945 kb on - strand, within MPMX20_00832 at 873.945 kb on - strand, within MPMX20_00832 at 873.971 kb on - strand, within MPMX20_00832 at 873.971 kb on - strand, within MPMX20_00832 at 873.971 kb on - strand, within MPMX20_00832 at 873.976 kb on - strand, within MPMX20_00832 at 873.976 kb on - strand, within MPMX20_00832 at 873.981 kb on - strand, within MPMX20_00832 at 873.999 kb on - strand, within MPMX20_00832 at 874.005 kb on + strand, within MPMX20_00832 at 874.007 kb on - strand, within MPMX20_00832 at 874.013 kb on - strand, within MPMX20_00832 at 874.092 kb on - strand, within MPMX20_00832 at 874.112 kb on - strand, within MPMX20_00832 at 874.125 kb on - strand, within MPMX20_00832 at 874.157 kb on + strand, within MPMX20_00832 at 874.166 kb on + strand, within MPMX20_00832 at 874.167 kb on + strand, within MPMX20_00832 at 874.175 kb on - strand, within MPMX20_00832 at 874.175 kb on - strand, within MPMX20_00832 at 874.175 kb on - strand, within MPMX20_00832 at 874.186 kb on + strand, within MPMX20_00832 at 874.186 kb on + strand, within MPMX20_00832 at 874.194 kb on - strand, within MPMX20_00832 at 874.194 kb on - strand, within MPMX20_00832 at 874.194 kb on - strand, within MPMX20_00832 at 874.194 kb on - strand, within MPMX20_00832 at 874.244 kb on + strand, within MPMX20_00832 at 874.271 kb on - strand, within MPMX20_00832 at 874.313 kb on - strand, within MPMX20_00832 at 874.340 kb on - strand, within MPMX20_00832 at 874.355 kb on + strand, within MPMX20_00832 at 874.355 kb on + strand, within MPMX20_00832 at 874.369 kb on - strand, within MPMX20_00832 at 874.370 kb on + strand, within MPMX20_00832 at 874.370 kb on + strand, within MPMX20_00832 at 874.374 kb on - strand, within MPMX20_00832 at 874.378 kb on - strand, within MPMX20_00832 at 874.378 kb on - strand, within MPMX20_00832 at 874.394 kb on - strand, within MPMX20_00832 at 874.404 kb on - strand at 874.434 kb on + strand at 874.480 kb on + strand at 874.510 kb on - strand at 874.528 kb on - strand, within MPMX20_00833 at 874.532 kb on + strand, within MPMX20_00833 at 874.540 kb on - strand, within MPMX20_00833 at 874.559 kb on + strand, within MPMX20_00833 at 874.560 kb on + strand, within MPMX20_00833 at 874.560 kb on + strand, within MPMX20_00833
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 872,138 - +0.8 872,187 - -1.4 872,328 + MPMX20_00830 0.16 +0.6 872,336 - MPMX20_00830 0.17 +3.6 872,356 + MPMX20_00830 0.20 +1.6 872,395 - MPMX20_00830 0.24 -1.4 872,402 - MPMX20_00830 0.25 -2.2 872,492 + MPMX20_00830 0.35 -2.2 872,492 + MPMX20_00830 0.35 -0.7 872,492 + MPMX20_00830 0.35 -1.6 872,500 - MPMX20_00830 0.36 +0.9 872,500 - MPMX20_00830 0.36 -2.3 872,515 - MPMX20_00830 0.37 -2.0 872,560 - MPMX20_00830 0.43 +0.4 872,690 + MPMX20_00830 0.57 -2.6 872,690 + MPMX20_00830 0.57 +0.6 872,693 - MPMX20_00830 0.57 +1.2 872,693 - MPMX20_00830 0.57 +1.2 872,698 - MPMX20_00830 0.58 +0.5 872,698 - MPMX20_00830 0.58 -0.2 872,698 - MPMX20_00830 0.58 +0.3 872,734 - MPMX20_00830 0.62 -0.5 872,760 + MPMX20_00830 0.65 -0.0 872,769 - MPMX20_00830 0.66 +0.1 872,895 + MPMX20_00830 0.80 -1.5 872,900 + MPMX20_00830 0.81 +0.1 872,974 - MPMX20_00830 0.89 -2.0 873,047 + +0.9 873,069 + -0.7 873,103 + +0.8 873,111 - -0.7 873,111 - -0.2 873,111 - -0.5 873,113 + -0.7 873,127 + -0.7 873,187 + MPMX20_00831 0.12 -0.4 873,188 - MPMX20_00831 0.12 +0.0 873,195 - MPMX20_00831 0.14 -2.0 873,329 + MPMX20_00831 0.43 -0.4 873,339 - MPMX20_00831 0.46 -2.3 873,364 + MPMX20_00831 0.51 -1.0 873,364 + MPMX20_00831 0.51 -1.4 873,470 - MPMX20_00831 0.74 -2.5 873,470 - MPMX20_00831 0.74 -1.3 873,484 - MPMX20_00831 0.77 -0.9 873,486 - MPMX20_00831 0.78 -1.2 873,596 + -0.1 873,608 + -0.8 873,608 + -0.1 873,616 - -0.2 873,616 - -0.4 873,617 + -3.2 873,650 - +0.0 873,650 - +0.4 873,683 + -0.7 873,691 + -0.6 873,691 - -0.1 873,691 - -0.3 873,693 + -0.4 873,693 + -1.6 873,693 + -0.6 873,737 - +0.2 873,762 - -1.1 873,802 - -0.3 873,826 + -0.4 873,827 + +0.0 873,834 + MPMX20_00832 0.10 +0.3 873,842 + MPMX20_00832 0.11 -0.3 873,930 - MPMX20_00832 0.24 -1.7 873,937 + MPMX20_00832 0.25 +0.2 873,937 + MPMX20_00832 0.25 -0.7 873,937 + MPMX20_00832 0.25 -1.4 873,945 - MPMX20_00832 0.26 -0.2 873,945 - MPMX20_00832 0.26 -0.5 873,945 - MPMX20_00832 0.26 -0.2 873,945 - MPMX20_00832 0.26 +0.0 873,945 - MPMX20_00832 0.26 +3.5 873,945 - MPMX20_00832 0.26 -0.4 873,971 - MPMX20_00832 0.30 -1.2 873,971 - MPMX20_00832 0.30 +0.1 873,971 - MPMX20_00832 0.30 +0.0 873,976 - MPMX20_00832 0.30 -0.7 873,976 - MPMX20_00832 0.30 -0.2 873,981 - MPMX20_00832 0.31 -1.5 873,999 - MPMX20_00832 0.33 -0.3 874,005 + MPMX20_00832 0.34 -0.1 874,007 - MPMX20_00832 0.35 +0.1 874,013 - MPMX20_00832 0.35 -0.2 874,092 - MPMX20_00832 0.47 -0.2 874,112 - MPMX20_00832 0.50 +0.8 874,125 - MPMX20_00832 0.51 +0.7 874,157 + MPMX20_00832 0.56 +1.3 874,166 + MPMX20_00832 0.57 +0.2 874,167 + MPMX20_00832 0.57 -0.1 874,175 - MPMX20_00832 0.58 +0.6 874,175 - MPMX20_00832 0.58 +0.4 874,175 - MPMX20_00832 0.58 -0.1 874,186 + MPMX20_00832 0.60 -0.4 874,186 + MPMX20_00832 0.60 -1.2 874,194 - MPMX20_00832 0.61 +0.0 874,194 - MPMX20_00832 0.61 -0.8 874,194 - MPMX20_00832 0.61 -0.7 874,194 - MPMX20_00832 0.61 +0.9 874,244 + MPMX20_00832 0.68 +1.1 874,271 - MPMX20_00832 0.72 -1.0 874,313 - MPMX20_00832 0.78 -0.8 874,340 - MPMX20_00832 0.82 -0.4 874,355 + MPMX20_00832 0.84 -0.7 874,355 + MPMX20_00832 0.84 -0.4 874,369 - MPMX20_00832 0.86 +0.3 874,370 + MPMX20_00832 0.86 +1.0 874,370 + MPMX20_00832 0.86 +0.6 874,374 - MPMX20_00832 0.87 +0.3 874,378 - MPMX20_00832 0.87 +0.4 874,378 - MPMX20_00832 0.87 -2.3 874,394 - MPMX20_00832 0.90 +1.6 874,404 - -0.8 874,434 + -0.1 874,480 + -0.5 874,510 - +0.3 874,528 - MPMX20_00833 0.13 -0.0 874,532 + MPMX20_00833 0.14 +0.6 874,540 - MPMX20_00833 0.15 -0.4 874,559 + MPMX20_00833 0.18 -0.6 874,560 + MPMX20_00833 0.19 +0.4 874,560 + MPMX20_00833 0.19 -1.3
Or see this region's nucleotide sequence