Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00419 and MPMX20_00420 are separated by 335 nucleotides MPMX20_00420 and MPMX20_00421 are separated by 42 nucleotides
MPMX20_00419: MPMX20_00419 - Aspartate ammonia-lyase, at 435,222 to 436,658
_00419
MPMX20_00420: MPMX20_00420 - hypothetical protein, at 436,994 to 437,458
_00420
MPMX20_00421: MPMX20_00421 - L-methionine/branched-chain amino acid exporter YjeH, at 437,501 to 438,739
_00421
Position (kb) 436
437
438 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 436.096 kb on - strand, within MPMX20_00419 at 436.127 kb on - strand, within MPMX20_00419 at 436.154 kb on + strand, within MPMX20_00419 at 436.154 kb on + strand, within MPMX20_00419 at 436.162 kb on - strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.193 kb on - strand, within MPMX20_00419 at 436.193 kb on - strand, within MPMX20_00419 at 436.209 kb on + strand, within MPMX20_00419 at 436.213 kb on + strand, within MPMX20_00419 at 436.271 kb on + strand, within MPMX20_00419 at 436.272 kb on + strand, within MPMX20_00419 at 436.306 kb on - strand, within MPMX20_00419 at 436.306 kb on - strand, within MPMX20_00419 at 436.306 kb on - strand, within MPMX20_00419 at 436.327 kb on - strand, within MPMX20_00419 at 436.330 kb on - strand, within MPMX20_00419 at 436.375 kb on + strand, within MPMX20_00419 at 436.404 kb on + strand, within MPMX20_00419 at 436.412 kb on - strand, within MPMX20_00419 at 436.588 kb on + strand at 436.588 kb on + strand at 436.596 kb on - strand at 436.637 kb on - strand at 436.656 kb on + strand at 436.664 kb on - strand at 436.690 kb on + strand at 436.698 kb on - strand at 436.715 kb on + strand at 436.745 kb on - strand at 436.777 kb on - strand at 436.779 kb on + strand at 436.779 kb on + strand at 436.793 kb on + strand at 436.793 kb on + strand at 436.793 kb on + strand at 436.816 kb on - strand at 436.853 kb on - strand at 436.913 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.961 kb on + strand at 436.961 kb on + strand at 436.961 kb on + strand at 436.961 kb on + strand at 436.969 kb on - strand at 436.969 kb on - strand at 436.969 kb on - strand at 436.969 kb on - strand at 436.976 kb on + strand at 436.984 kb on + strand at 436.984 kb on + strand at 436.992 kb on - strand at 436.992 kb on - strand at 436.992 kb on - strand at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.076 kb on - strand, within MPMX20_00420 at 437.076 kb on - strand, within MPMX20_00420 at 437.268 kb on - strand, within MPMX20_00420 at 437.270 kb on + strand, within MPMX20_00420 at 437.362 kb on - strand, within MPMX20_00420 at 437.374 kb on - strand, within MPMX20_00420 at 437.378 kb on + strand, within MPMX20_00420 at 437.381 kb on - strand, within MPMX20_00420 at 437.415 kb on + strand at 437.418 kb on - strand at 437.418 kb on - strand at 437.423 kb on - strand at 437.423 kb on - strand at 437.466 kb on - strand at 437.476 kb on - strand at 437.494 kb on - strand at 437.506 kb on - strand at 437.607 kb on + strand at 437.637 kb on + strand, within MPMX20_00421 at 437.652 kb on - strand, within MPMX20_00421 at 437.671 kb on + strand, within MPMX20_00421 at 437.713 kb on + strand, within MPMX20_00421 at 437.730 kb on - strand, within MPMX20_00421 at 437.745 kb on - strand, within MPMX20_00421 at 437.763 kb on + strand, within MPMX20_00421 at 437.776 kb on - strand, within MPMX20_00421 at 437.776 kb on - strand, within MPMX20_00421 at 437.786 kb on - strand, within MPMX20_00421 at 437.786 kb on - strand, within MPMX20_00421 at 437.805 kb on + strand, within MPMX20_00421 at 437.805 kb on + strand, within MPMX20_00421 at 437.805 kb on + strand, within MPMX20_00421 at 437.813 kb on - strand, within MPMX20_00421 at 437.848 kb on + strand, within MPMX20_00421 at 437.853 kb on + strand, within MPMX20_00421 at 437.858 kb on + strand, within MPMX20_00421 at 437.863 kb on + strand, within MPMX20_00421 at 437.873 kb on + strand, within MPMX20_00421 at 437.881 kb on - strand, within MPMX20_00421 at 437.896 kb on + strand, within MPMX20_00421 at 438.031 kb on + strand, within MPMX20_00421 at 438.033 kb on + strand, within MPMX20_00421 at 438.033 kb on + strand, within MPMX20_00421 at 438.033 kb on + strand, within MPMX20_00421 at 438.041 kb on - strand, within MPMX20_00421 at 438.041 kb on - strand, within MPMX20_00421 at 438.057 kb on + strand, within MPMX20_00421 at 438.057 kb on + strand, within MPMX20_00421 at 438.065 kb on - strand, within MPMX20_00421 at 438.136 kb on + strand, within MPMX20_00421 at 438.136 kb on + strand, within MPMX20_00421 at 438.144 kb on - strand, within MPMX20_00421 at 438.202 kb on + strand, within MPMX20_00421 at 438.210 kb on - strand, within MPMX20_00421 at 438.220 kb on - strand, within MPMX20_00421 at 438.321 kb on - strand, within MPMX20_00421 at 438.321 kb on - strand, within MPMX20_00421 at 438.433 kb on - strand, within MPMX20_00421 at 438.433 kb on - strand, within MPMX20_00421
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 435,997 - MPMX20_00419 0.54 +2.1 435,997 - MPMX20_00419 0.54 -2.7 435,997 - MPMX20_00419 0.54 -1.1 435,997 - MPMX20_00419 0.54 +1.1 435,997 - MPMX20_00419 0.54 -1.6 436,096 - MPMX20_00419 0.61 -0.3 436,127 - MPMX20_00419 0.63 +2.1 436,154 + MPMX20_00419 0.65 -2.2 436,154 + MPMX20_00419 0.65 -2.3 436,162 - MPMX20_00419 0.65 -0.3 436,185 + MPMX20_00419 0.67 -0.9 436,185 + MPMX20_00419 0.67 -2.0 436,185 + MPMX20_00419 0.67 -4.0 436,185 + MPMX20_00419 0.67 -3.2 436,185 + MPMX20_00419 0.67 -0.9 436,185 + MPMX20_00419 0.67 +0.5 436,193 - MPMX20_00419 0.68 -1.1 436,193 - MPMX20_00419 0.68 -2.3 436,209 + MPMX20_00419 0.69 -0.6 436,213 + MPMX20_00419 0.69 -4.1 436,271 + MPMX20_00419 0.73 -2.9 436,272 + MPMX20_00419 0.73 -1.1 436,306 - MPMX20_00419 0.75 -1.6 436,306 - MPMX20_00419 0.75 +2.1 436,306 - MPMX20_00419 0.75 -2.7 436,327 - MPMX20_00419 0.77 -3.6 436,330 - MPMX20_00419 0.77 -3.3 436,375 + MPMX20_00419 0.80 -2.6 436,404 + MPMX20_00419 0.82 +1.1 436,412 - MPMX20_00419 0.83 -0.3 436,588 + -1.6 436,588 + -2.8 436,596 - -2.5 436,637 - -1.4 436,656 + -2.3 436,664 - -0.5 436,690 + -2.2 436,698 - -1.3 436,715 + -2.3 436,745 - -1.0 436,777 - +0.3 436,779 + +0.1 436,779 + -0.3 436,793 + -2.1 436,793 + -0.5 436,793 + +1.6 436,816 - +0.2 436,853 - -0.8 436,913 + +1.5 436,949 + -0.5 436,949 + +0.6 436,949 + +0.1 436,949 + +0.2 436,949 + -1.0 436,949 + -0.3 436,949 + -0.2 436,949 + -0.9 436,949 + -1.8 436,949 + -0.3 436,957 - -0.5 436,957 - -0.9 436,957 - -2.5 436,957 - -0.5 436,957 - +0.5 436,957 - -0.8 436,957 - -1.5 436,957 - -1.0 436,957 - -0.9 436,957 - -0.2 436,957 - -0.9 436,957 - -0.2 436,957 - -0.3 436,957 - +0.2 436,957 - +3.7 436,957 - -1.5 436,957 - -0.3 436,957 - -1.1 436,957 - +0.7 436,961 + -0.8 436,961 + -0.5 436,961 + -0.9 436,961 + +1.5 436,969 - +0.1 436,969 - +1.7 436,969 - +0.6 436,969 - -0.8 436,976 + -1.1 436,984 + -1.7 436,984 + -0.6 436,992 - -1.1 436,992 - -0.1 436,992 - -0.3 437,064 + MPMX20_00420 0.15 -0.9 437,064 + MPMX20_00420 0.15 -0.2 437,064 + MPMX20_00420 0.15 -1.3 437,064 + MPMX20_00420 0.15 -0.4 437,064 + MPMX20_00420 0.15 +3.6 437,064 + MPMX20_00420 0.15 -0.9 437,064 + MPMX20_00420 0.15 -0.3 437,064 + MPMX20_00420 0.15 -1.3 437,064 + MPMX20_00420 0.15 -0.4 437,064 + MPMX20_00420 0.15 -0.8 437,064 + MPMX20_00420 0.15 -1.0 437,064 + MPMX20_00420 0.15 -0.3 437,064 + MPMX20_00420 0.15 -0.3 437,064 + MPMX20_00420 0.15 +0.1 437,064 + MPMX20_00420 0.15 +0.1 437,064 + MPMX20_00420 0.15 -3.5 437,072 - MPMX20_00420 0.17 +0.1 437,072 - MPMX20_00420 0.17 +4.4 437,072 - MPMX20_00420 0.17 -0.6 437,072 - MPMX20_00420 0.17 +0.6 437,072 - MPMX20_00420 0.17 +1.1 437,072 - MPMX20_00420 0.17 -0.4 437,072 - MPMX20_00420 0.17 +0.2 437,072 - MPMX20_00420 0.17 -1.1 437,072 - MPMX20_00420 0.17 -1.3 437,072 - MPMX20_00420 0.17 -0.7 437,072 - MPMX20_00420 0.17 -0.8 437,076 - MPMX20_00420 0.18 +1.1 437,076 - MPMX20_00420 0.18 -1.1 437,268 - MPMX20_00420 0.59 -0.8 437,270 + MPMX20_00420 0.59 -1.6 437,362 - MPMX20_00420 0.79 -0.5 437,374 - MPMX20_00420 0.82 +1.1 437,378 + MPMX20_00420 0.83 +0.8 437,381 - MPMX20_00420 0.83 +0.2 437,415 + +0.5 437,418 - -1.2 437,418 - +0.7 437,423 - -1.4 437,423 - -0.3 437,466 - +0.1 437,476 - +0.5 437,494 - -1.5 437,506 - -1.3 437,607 + +0.5 437,637 + MPMX20_00421 0.11 +0.4 437,652 - MPMX20_00421 0.12 -1.1 437,671 + MPMX20_00421 0.14 -0.1 437,713 + MPMX20_00421 0.17 -1.8 437,730 - MPMX20_00421 0.18 -0.7 437,745 - MPMX20_00421 0.20 +0.7 437,763 + MPMX20_00421 0.21 +0.5 437,776 - MPMX20_00421 0.22 -0.5 437,776 - MPMX20_00421 0.22 -0.0 437,786 - MPMX20_00421 0.23 +0.1 437,786 - MPMX20_00421 0.23 +1.2 437,805 + MPMX20_00421 0.25 +0.5 437,805 + MPMX20_00421 0.25 +1.6 437,805 + MPMX20_00421 0.25 -2.4 437,813 - MPMX20_00421 0.25 -0.9 437,848 + MPMX20_00421 0.28 +2.7 437,853 + MPMX20_00421 0.28 -2.8 437,858 + MPMX20_00421 0.29 -0.4 437,863 + MPMX20_00421 0.29 -0.5 437,873 + MPMX20_00421 0.30 -0.4 437,881 - MPMX20_00421 0.31 -0.2 437,896 + MPMX20_00421 0.32 -0.4 438,031 + MPMX20_00421 0.43 +0.1 438,033 + MPMX20_00421 0.43 -1.1 438,033 + MPMX20_00421 0.43 +0.2 438,033 + MPMX20_00421 0.43 -0.1 438,041 - MPMX20_00421 0.44 -0.6 438,041 - MPMX20_00421 0.44 -0.7 438,057 + MPMX20_00421 0.45 -0.4 438,057 + MPMX20_00421 0.45 +0.7 438,065 - MPMX20_00421 0.46 -0.9 438,136 + MPMX20_00421 0.51 +3.9 438,136 + MPMX20_00421 0.51 -0.3 438,144 - MPMX20_00421 0.52 -2.1 438,202 + MPMX20_00421 0.57 +0.5 438,210 - MPMX20_00421 0.57 -0.4 438,220 - MPMX20_00421 0.58 +1.1 438,321 - MPMX20_00421 0.66 +1.1 438,321 - MPMX20_00421 0.66 +1.1 438,433 - MPMX20_00421 0.75 +2.4 438,433 - MPMX20_00421 0.75 +0.5
Or see this region's nucleotide sequence