Experiment: 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01052 and MPMX20_01053 are separated by 33 nucleotides MPMX20_01053 and MPMX20_01054 are separated by 95 nucleotides MPMX20_01054 and MPMX20_01055 overlap by 8 nucleotides
MPMX20_01052: MPMX20_01052 - Beta-galactosidase, at 1,107,019 to 1,110,111
_01052
MPMX20_01053: MPMX20_01053 - Nickel/cobalt efflux system RcnA, at 1,110,145 to 1,110,963
_01053
MPMX20_01054: MPMX20_01054 - Transcriptional repressor RcnR, at 1,111,059 to 1,111,370
_01054
MPMX20_01055: MPMX20_01055 - putative cyclic di-GMP phosphodiesterase PdeB, at 1,111,363 to 1,112,928
_01055
Position (kb)
1111
1112 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1110.104 kb on - strand at 1110.104 kb on - strand at 1110.148 kb on + strand at 1110.153 kb on + strand at 1110.156 kb on - strand at 1110.156 kb on - strand at 1110.177 kb on - strand at 1110.199 kb on + strand at 1110.199 kb on + strand at 1110.207 kb on - strand at 1110.207 kb on - strand at 1110.285 kb on - strand, within MPMX20_01053 at 1110.353 kb on - strand, within MPMX20_01053 at 1110.353 kb on - strand, within MPMX20_01053 at 1110.422 kb on - strand, within MPMX20_01053 at 1110.463 kb on - strand, within MPMX20_01053 at 1110.491 kb on - strand, within MPMX20_01053 at 1110.592 kb on - strand, within MPMX20_01053 at 1110.616 kb on - strand, within MPMX20_01053 at 1110.616 kb on - strand, within MPMX20_01053 at 1110.655 kb on - strand, within MPMX20_01053 at 1110.664 kb on - strand, within MPMX20_01053 at 1110.688 kb on - strand, within MPMX20_01053 at 1110.703 kb on - strand, within MPMX20_01053 at 1110.710 kb on - strand, within MPMX20_01053 at 1110.715 kb on - strand, within MPMX20_01053 at 1110.763 kb on + strand, within MPMX20_01053 at 1110.799 kb on + strand, within MPMX20_01053 at 1110.857 kb on + strand, within MPMX20_01053 at 1110.857 kb on + strand, within MPMX20_01053 at 1110.865 kb on - strand, within MPMX20_01053 at 1110.866 kb on - strand, within MPMX20_01053 at 1110.873 kb on + strand, within MPMX20_01053 at 1110.881 kb on - strand, within MPMX20_01053 at 1110.900 kb on + strand at 1110.908 kb on - strand at 1110.921 kb on + strand at 1110.934 kb on - strand at 1110.934 kb on - strand at 1110.986 kb on - strand at 1111.083 kb on - strand at 1111.162 kb on + strand, within MPMX20_01054 at 1111.179 kb on + strand, within MPMX20_01054 at 1111.255 kb on - strand, within MPMX20_01054 at 1111.301 kb on + strand, within MPMX20_01054 at 1111.324 kb on - strand, within MPMX20_01054 at 1111.338 kb on + strand, within MPMX20_01054 at 1111.401 kb on + strand at 1111.432 kb on - strand at 1111.435 kb on + strand at 1111.435 kb on + strand at 1111.435 kb on + strand at 1111.438 kb on - strand at 1111.446 kb on + strand at 1111.568 kb on - strand, within MPMX20_01055 at 1111.638 kb on + strand, within MPMX20_01055 at 1111.638 kb on + strand, within MPMX20_01055 at 1111.638 kb on + strand, within MPMX20_01055 at 1111.638 kb on + strand, within MPMX20_01055 at 1111.638 kb on + strand, within MPMX20_01055 at 1111.642 kb on + strand, within MPMX20_01055 at 1111.642 kb on + strand, within MPMX20_01055 at 1111.646 kb on - strand, within MPMX20_01055 at 1111.646 kb on - strand, within MPMX20_01055 at 1111.646 kb on - strand, within MPMX20_01055 at 1111.646 kb on - strand, within MPMX20_01055 at 1111.646 kb on - strand, within MPMX20_01055 at 1111.650 kb on - strand, within MPMX20_01055 at 1111.749 kb on + strand, within MPMX20_01055 at 1111.757 kb on - strand, within MPMX20_01055 at 1111.757 kb on - strand, within MPMX20_01055 at 1111.806 kb on - strand, within MPMX20_01055 at 1111.868 kb on + strand, within MPMX20_01055 at 1111.868 kb on + strand, within MPMX20_01055 at 1111.875 kb on + strand, within MPMX20_01055 at 1111.876 kb on - strand, within MPMX20_01055 at 1111.876 kb on - strand, within MPMX20_01055 at 1111.883 kb on - strand, within MPMX20_01055 at 1111.896 kb on + strand, within MPMX20_01055 at 1112.005 kb on - strand, within MPMX20_01055 at 1112.005 kb on - strand, within MPMX20_01055 at 1112.009 kb on + strand, within MPMX20_01055 at 1112.035 kb on - strand, within MPMX20_01055 at 1112.047 kb on - strand, within MPMX20_01055 at 1112.166 kb on + strand, within MPMX20_01055 at 1112.202 kb on - strand, within MPMX20_01055 at 1112.229 kb on + strand, within MPMX20_01055 at 1112.253 kb on + strand, within MPMX20_01055 at 1112.307 kb on + strand, within MPMX20_01055 at 1112.321 kb on + strand, within MPMX20_01055 at 1112.323 kb on - strand, within MPMX20_01055 at 1112.335 kb on - strand, within MPMX20_01055 at 1112.335 kb on - strand, within MPMX20_01055
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 1,110,104 - +1.1 1,110,104 - -1.7 1,110,148 + -2.5 1,110,153 + -1.5 1,110,156 - +0.2 1,110,156 - -1.1 1,110,177 - +0.4 1,110,199 + -0.1 1,110,199 + -1.0 1,110,207 - -1.8 1,110,207 - -1.7 1,110,285 - MPMX20_01053 0.17 -2.2 1,110,353 - MPMX20_01053 0.25 -1.1 1,110,353 - MPMX20_01053 0.25 -1.4 1,110,422 - MPMX20_01053 0.34 -0.6 1,110,463 - MPMX20_01053 0.39 -0.7 1,110,491 - MPMX20_01053 0.42 -1.3 1,110,592 - MPMX20_01053 0.55 -0.2 1,110,616 - MPMX20_01053 0.58 +0.2 1,110,616 - MPMX20_01053 0.58 -0.3 1,110,655 - MPMX20_01053 0.62 -0.3 1,110,664 - MPMX20_01053 0.63 +0.1 1,110,688 - MPMX20_01053 0.66 +0.7 1,110,703 - MPMX20_01053 0.68 -0.9 1,110,710 - MPMX20_01053 0.69 -1.6 1,110,715 - MPMX20_01053 0.70 -0.4 1,110,763 + MPMX20_01053 0.75 +1.2 1,110,799 + MPMX20_01053 0.80 +0.4 1,110,857 + MPMX20_01053 0.87 -0.4 1,110,857 + MPMX20_01053 0.87 +0.1 1,110,865 - MPMX20_01053 0.88 -0.1 1,110,866 - MPMX20_01053 0.88 -1.1 1,110,873 + MPMX20_01053 0.89 -0.9 1,110,881 - MPMX20_01053 0.90 -1.5 1,110,900 + +1.0 1,110,908 - -1.3 1,110,921 + -0.3 1,110,934 - +2.1 1,110,934 - +1.1 1,110,986 - +1.7 1,111,083 - +0.1 1,111,162 + MPMX20_01054 0.33 -2.0 1,111,179 + MPMX20_01054 0.38 -0.3 1,111,255 - MPMX20_01054 0.63 -1.8 1,111,301 + MPMX20_01054 0.78 -0.8 1,111,324 - MPMX20_01054 0.85 +0.2 1,111,338 + MPMX20_01054 0.89 -1.1 1,111,401 + -0.8 1,111,432 - -0.6 1,111,435 + -0.1 1,111,435 + -0.9 1,111,435 + -0.1 1,111,438 - -2.7 1,111,446 + -0.9 1,111,568 - MPMX20_01055 0.13 +0.4 1,111,638 + MPMX20_01055 0.18 +0.8 1,111,638 + MPMX20_01055 0.18 +0.8 1,111,638 + MPMX20_01055 0.18 -0.4 1,111,638 + MPMX20_01055 0.18 +1.1 1,111,638 + MPMX20_01055 0.18 -0.3 1,111,642 + MPMX20_01055 0.18 -1.4 1,111,642 + MPMX20_01055 0.18 -0.3 1,111,646 - MPMX20_01055 0.18 +1.1 1,111,646 - MPMX20_01055 0.18 -2.3 1,111,646 - MPMX20_01055 0.18 -0.5 1,111,646 - MPMX20_01055 0.18 -0.9 1,111,646 - MPMX20_01055 0.18 -0.3 1,111,650 - MPMX20_01055 0.18 -0.8 1,111,749 + MPMX20_01055 0.25 +0.0 1,111,757 - MPMX20_01055 0.25 -1.4 1,111,757 - MPMX20_01055 0.25 +1.5 1,111,806 - MPMX20_01055 0.28 +1.5 1,111,868 + MPMX20_01055 0.32 -1.2 1,111,868 + MPMX20_01055 0.32 -0.1 1,111,875 + MPMX20_01055 0.33 +1.4 1,111,876 - MPMX20_01055 0.33 -0.4 1,111,876 - MPMX20_01055 0.33 +0.4 1,111,883 - MPMX20_01055 0.33 +0.4 1,111,896 + MPMX20_01055 0.34 -0.6 1,112,005 - MPMX20_01055 0.41 -1.0 1,112,005 - MPMX20_01055 0.41 -1.0 1,112,009 + MPMX20_01055 0.41 -1.1 1,112,035 - MPMX20_01055 0.43 -1.5 1,112,047 - MPMX20_01055 0.44 -0.2 1,112,166 + MPMX20_01055 0.51 -0.8 1,112,202 - MPMX20_01055 0.54 +2.7 1,112,229 + MPMX20_01055 0.55 -1.1 1,112,253 + MPMX20_01055 0.57 -0.3 1,112,307 + MPMX20_01055 0.60 -1.3 1,112,321 + MPMX20_01055 0.61 -1.1 1,112,323 - MPMX20_01055 0.61 -3.1 1,112,335 - MPMX20_01055 0.62 +0.4 1,112,335 - MPMX20_01055 0.62 -1.4
Or see this region's nucleotide sequence