Experiment: 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00501 and MPMX20_00502 are separated by 0 nucleotides MPMX20_00502 and MPMX20_00503 are separated by 225 nucleotides MPMX20_00503 and MPMX20_00504 overlap by 4 nucleotides
MPMX20_00501: MPMX20_00501 - HTH-type transcriptional repressor CytR, at 515,579 to 516,637
_00501
MPMX20_00502: MPMX20_00502 - Peptide methionine sulfoxide reductase MsrA, at 516,638 to 517,279
_00502
MPMX20_00503: MPMX20_00503 - Translocation and assembly module subunit TamA, at 517,505 to 519,238
_00503
MPMX20_00504: MPMX20_00504 - Translocation and assembly module subunit TamB, at 519,235 to 523,011
_00504
Position (kb) 517
518
519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 516.592 kb on + strand at 516.631 kb on - strand at 516.671 kb on + strand at 516.773 kb on - strand, within MPMX20_00502 at 516.794 kb on + strand, within MPMX20_00502 at 516.822 kb on - strand, within MPMX20_00502 at 516.822 kb on - strand, within MPMX20_00502 at 516.834 kb on + strand, within MPMX20_00502 at 516.834 kb on + strand, within MPMX20_00502 at 516.852 kb on - strand, within MPMX20_00502 at 516.868 kb on - strand, within MPMX20_00502 at 516.875 kb on + strand, within MPMX20_00502 at 516.875 kb on + strand, within MPMX20_00502 at 516.883 kb on - strand, within MPMX20_00502 at 516.911 kb on - strand, within MPMX20_00502 at 516.921 kb on - strand, within MPMX20_00502 at 516.927 kb on - strand, within MPMX20_00502 at 516.946 kb on - strand, within MPMX20_00502 at 516.952 kb on + strand, within MPMX20_00502 at 516.952 kb on + strand, within MPMX20_00502 at 516.967 kb on + strand, within MPMX20_00502 at 516.974 kb on + strand, within MPMX20_00502 at 516.974 kb on + strand, within MPMX20_00502 at 516.974 kb on + strand, within MPMX20_00502 at 516.974 kb on + strand, within MPMX20_00502 at 516.974 kb on + strand, within MPMX20_00502 at 516.975 kb on - strand, within MPMX20_00502 at 516.980 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 516.982 kb on - strand, within MPMX20_00502 at 517.001 kb on - strand, within MPMX20_00502 at 517.001 kb on - strand, within MPMX20_00502 at 517.005 kb on - strand, within MPMX20_00502 at 517.012 kb on + strand, within MPMX20_00502 at 517.020 kb on - strand, within MPMX20_00502 at 517.020 kb on - strand, within MPMX20_00502 at 517.020 kb on - strand, within MPMX20_00502 at 517.045 kb on + strand, within MPMX20_00502 at 517.076 kb on + strand, within MPMX20_00502 at 517.191 kb on + strand, within MPMX20_00502 at 517.193 kb on + strand, within MPMX20_00502 at 517.217 kb on - strand at 517.217 kb on - strand at 517.218 kb on - strand at 517.218 kb on - strand at 517.223 kb on - strand at 517.304 kb on + strand at 517.309 kb on + strand at 517.315 kb on + strand at 517.327 kb on + strand at 517.327 kb on + strand at 517.350 kb on - strand at 517.358 kb on - strand at 517.361 kb on + strand at 517.369 kb on - strand at 517.401 kb on + strand at 517.460 kb on - strand at 517.544 kb on - strand at 517.553 kb on + strand at 517.568 kb on + strand at 517.585 kb on + strand at 517.586 kb on + strand at 517.594 kb on - strand at 517.599 kb on - strand at 517.630 kb on + strand at 517.630 kb on + strand at 517.630 kb on + strand at 517.667 kb on - strand at 517.872 kb on + strand, within MPMX20_00503 at 517.872 kb on + strand, within MPMX20_00503 at 517.872 kb on + strand, within MPMX20_00503 at 517.872 kb on + strand, within MPMX20_00503 at 517.880 kb on - strand, within MPMX20_00503 at 517.885 kb on - strand, within MPMX20_00503 at 517.925 kb on + strand, within MPMX20_00503 at 517.956 kb on + strand, within MPMX20_00503 at 517.956 kb on + strand, within MPMX20_00503 at 517.956 kb on - strand, within MPMX20_00503 at 517.966 kb on + strand, within MPMX20_00503 at 517.966 kb on + strand, within MPMX20_00503 at 518.003 kb on + strand, within MPMX20_00503 at 518.005 kb on + strand, within MPMX20_00503 at 518.013 kb on - strand, within MPMX20_00503 at 518.026 kb on + strand, within MPMX20_00503 at 518.035 kb on - strand, within MPMX20_00503 at 518.089 kb on - strand, within MPMX20_00503 at 518.127 kb on + strand, within MPMX20_00503 at 518.135 kb on - strand, within MPMX20_00503 at 518.171 kb on - strand, within MPMX20_00503 at 518.177 kb on + strand, within MPMX20_00503 at 518.388 kb on + strand, within MPMX20_00503 at 518.396 kb on - strand, within MPMX20_00503 at 518.480 kb on + strand, within MPMX20_00503 at 518.480 kb on - strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.482 kb on + strand, within MPMX20_00503 at 518.487 kb on + strand, within MPMX20_00503 at 518.490 kb on - strand, within MPMX20_00503 at 518.490 kb on - strand, within MPMX20_00503 at 518.490 kb on - strand, within MPMX20_00503 at 518.490 kb on - strand, within MPMX20_00503 at 518.490 kb on - strand, within MPMX20_00503 at 518.502 kb on + strand, within MPMX20_00503 at 518.565 kb on + strand, within MPMX20_00503 at 518.595 kb on + strand, within MPMX20_00503 at 518.595 kb on + strand, within MPMX20_00503 at 518.595 kb on + strand, within MPMX20_00503 at 518.595 kb on + strand, within MPMX20_00503 at 518.603 kb on - strand, within MPMX20_00503 at 518.603 kb on - strand, within MPMX20_00503 at 518.603 kb on - strand, within MPMX20_00503 at 518.603 kb on - strand, within MPMX20_00503 at 518.605 kb on + strand, within MPMX20_00503 at 518.612 kb on + strand, within MPMX20_00503 at 518.613 kb on + strand, within MPMX20_00503 at 518.622 kb on + strand, within MPMX20_00503 at 518.627 kb on + strand, within MPMX20_00503 at 518.630 kb on - strand, within MPMX20_00503 at 518.630 kb on - strand, within MPMX20_00503 at 518.630 kb on - strand, within MPMX20_00503 at 518.630 kb on - strand, within MPMX20_00503 at 518.635 kb on - strand, within MPMX20_00503 at 518.635 kb on - strand, within MPMX20_00503 at 518.657 kb on - strand, within MPMX20_00503 at 518.659 kb on + strand, within MPMX20_00503 at 518.664 kb on + strand, within MPMX20_00503 at 518.664 kb on + strand, within MPMX20_00503 at 518.672 kb on - strand, within MPMX20_00503 at 518.741 kb on + strand, within MPMX20_00503 at 518.749 kb on + strand, within MPMX20_00503 at 518.757 kb on - strand, within MPMX20_00503 at 518.757 kb on - strand, within MPMX20_00503 at 518.774 kb on - strand, within MPMX20_00503 at 518.821 kb on + strand, within MPMX20_00503 at 518.827 kb on - strand, within MPMX20_00503 at 518.863 kb on + strand, within MPMX20_00503 at 518.897 kb on + strand, within MPMX20_00503 at 518.991 kb on + strand, within MPMX20_00503 at 519.023 kb on - strand, within MPMX20_00503 at 519.025 kb on + strand, within MPMX20_00503 at 519.077 kb on + strand at 519.111 kb on - strand at 519.139 kb on + strand at 519.202 kb on - strand at 519.208 kb on + strand at 519.216 kb on - strand at 519.226 kb on - strand at 519.257 kb on + strand at 519.265 kb on - strand at 519.288 kb on + strand at 519.320 kb on + strand at 519.328 kb on - strand at 519.387 kb on - strand at 519.392 kb on - strand at 519.505 kb on - strand at 519.620 kb on - strand, within MPMX20_00504 at 519.644 kb on - strand, within MPMX20_00504 at 519.646 kb on + strand, within MPMX20_00504 at 519.646 kb on + strand, within MPMX20_00504 at 519.649 kb on - strand, within MPMX20_00504 at 519.654 kb on - strand, within MPMX20_00504 at 519.752 kb on + strand, within MPMX20_00504 at 519.757 kb on - strand, within MPMX20_00504 at 519.777 kb on + strand, within MPMX20_00504 at 519.865 kb on - strand, within MPMX20_00504 at 519.942 kb on + strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.950 kb on - strand, within MPMX20_00504 at 519.960 kb on - strand, within MPMX20_00504 at 519.988 kb on + strand, within MPMX20_00504 at 519.988 kb on - strand, within MPMX20_00504 at 519.990 kb on + strand, within MPMX20_00504 at 519.996 kb on - strand, within MPMX20_00504 at 520.002 kb on + strand, within MPMX20_00504 at 520.063 kb on - strand, within MPMX20_00504 at 520.116 kb on + strand, within MPMX20_00504 at 520.118 kb on + strand, within MPMX20_00504 at 520.118 kb on + strand, within MPMX20_00504 at 520.123 kb on + strand, within MPMX20_00504 at 520.123 kb on + strand, within MPMX20_00504 at 520.125 kb on - strand, within MPMX20_00504 at 520.173 kb on + strand, within MPMX20_00504 at 520.181 kb on - strand, within MPMX20_00504 at 520.182 kb on + strand, within MPMX20_00504
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 516,592 + -1.1 516,631 - -0.9 516,671 + +2.0 516,773 - MPMX20_00502 0.21 -1.1 516,794 + MPMX20_00502 0.24 -0.1 516,822 - MPMX20_00502 0.29 -0.2 516,822 - MPMX20_00502 0.29 +1.0 516,834 + MPMX20_00502 0.31 +1.0 516,834 + MPMX20_00502 0.31 +0.7 516,852 - MPMX20_00502 0.33 +1.0 516,868 - MPMX20_00502 0.36 -0.3 516,875 + MPMX20_00502 0.37 -0.5 516,875 + MPMX20_00502 0.37 -0.7 516,883 - MPMX20_00502 0.38 -0.1 516,911 - MPMX20_00502 0.43 +0.0 516,921 - MPMX20_00502 0.44 +1.6 516,927 - MPMX20_00502 0.45 -0.2 516,946 - MPMX20_00502 0.48 +0.8 516,952 + MPMX20_00502 0.49 +1.6 516,952 + MPMX20_00502 0.49 -1.5 516,967 + MPMX20_00502 0.51 -0.6 516,974 + MPMX20_00502 0.52 +0.0 516,974 + MPMX20_00502 0.52 +0.0 516,974 + MPMX20_00502 0.52 +1.0 516,974 + MPMX20_00502 0.52 -1.4 516,974 + MPMX20_00502 0.52 +0.3 516,975 - MPMX20_00502 0.52 -0.1 516,980 - MPMX20_00502 0.53 -0.4 516,982 - MPMX20_00502 0.54 -0.1 516,982 - MPMX20_00502 0.54 +1.6 516,982 - MPMX20_00502 0.54 +0.0 516,982 - MPMX20_00502 0.54 +0.3 516,982 - MPMX20_00502 0.54 -1.5 516,982 - MPMX20_00502 0.54 -0.5 516,982 - MPMX20_00502 0.54 -0.4 517,001 - MPMX20_00502 0.57 +0.2 517,001 - MPMX20_00502 0.57 +0.0 517,005 - MPMX20_00502 0.57 -0.2 517,012 + MPMX20_00502 0.58 -0.9 517,020 - MPMX20_00502 0.60 +0.5 517,020 - MPMX20_00502 0.60 +0.2 517,020 - MPMX20_00502 0.60 -0.6 517,045 + MPMX20_00502 0.63 +1.0 517,076 + MPMX20_00502 0.68 -0.1 517,191 + MPMX20_00502 0.86 +2.0 517,193 + MPMX20_00502 0.86 +0.6 517,217 - -1.5 517,217 - -0.4 517,218 - -0.3 517,218 - +1.0 517,223 - -0.6 517,304 + +0.6 517,309 + +0.8 517,315 + -0.2 517,327 + -0.1 517,327 + -1.6 517,350 - -0.6 517,358 - +3.2 517,361 + +0.8 517,369 - +1.0 517,401 + -1.5 517,460 - +0.3 517,544 - -2.0 517,553 + -2.1 517,568 + -0.7 517,585 + -0.3 517,586 + +1.4 517,594 - +1.6 517,599 - -0.8 517,630 + -0.2 517,630 + -1.3 517,630 + -1.8 517,667 - +0.0 517,872 + MPMX20_00503 0.21 -2.0 517,872 + MPMX20_00503 0.21 -0.8 517,872 + MPMX20_00503 0.21 -1.8 517,872 + MPMX20_00503 0.21 -1.7 517,880 - MPMX20_00503 0.22 -0.4 517,885 - MPMX20_00503 0.22 -2.1 517,925 + MPMX20_00503 0.24 -1.6 517,956 + MPMX20_00503 0.26 +1.6 517,956 + MPMX20_00503 0.26 -0.2 517,956 - MPMX20_00503 0.26 -1.5 517,966 + MPMX20_00503 0.27 -0.5 517,966 + MPMX20_00503 0.27 -2.0 518,003 + MPMX20_00503 0.29 -0.8 518,005 + MPMX20_00503 0.29 -1.3 518,013 - MPMX20_00503 0.29 -1.1 518,026 + MPMX20_00503 0.30 -1.3 518,035 - MPMX20_00503 0.31 -0.8 518,089 - MPMX20_00503 0.34 -3.0 518,127 + MPMX20_00503 0.36 +0.4 518,135 - MPMX20_00503 0.36 -2.2 518,171 - MPMX20_00503 0.38 -0.7 518,177 + MPMX20_00503 0.39 +2.0 518,388 + MPMX20_00503 0.51 -0.5 518,396 - MPMX20_00503 0.51 -2.6 518,480 + MPMX20_00503 0.56 -2.1 518,480 - MPMX20_00503 0.56 -1.2 518,482 + MPMX20_00503 0.56 +3.0 518,482 + MPMX20_00503 0.56 -2.1 518,482 + MPMX20_00503 0.56 -1.9 518,482 + MPMX20_00503 0.56 -2.8 518,482 + MPMX20_00503 0.56 -3.0 518,482 + MPMX20_00503 0.56 -1.4 518,482 + MPMX20_00503 0.56 -1.7 518,482 + MPMX20_00503 0.56 -2.0 518,482 + MPMX20_00503 0.56 -1.3 518,487 + MPMX20_00503 0.57 -1.2 518,490 - MPMX20_00503 0.57 -0.5 518,490 - MPMX20_00503 0.57 -0.3 518,490 - MPMX20_00503 0.57 -1.0 518,490 - MPMX20_00503 0.57 -0.7 518,490 - MPMX20_00503 0.57 +1.0 518,502 + MPMX20_00503 0.57 -1.4 518,565 + MPMX20_00503 0.61 -2.2 518,595 + MPMX20_00503 0.63 -1.6 518,595 + MPMX20_00503 0.63 -0.7 518,595 + MPMX20_00503 0.63 +1.0 518,595 + MPMX20_00503 0.63 -1.3 518,603 - MPMX20_00503 0.63 -1.2 518,603 - MPMX20_00503 0.63 -0.8 518,603 - MPMX20_00503 0.63 +0.0 518,603 - MPMX20_00503 0.63 -2.4 518,605 + MPMX20_00503 0.63 -1.9 518,612 + MPMX20_00503 0.64 -2.1 518,613 + MPMX20_00503 0.64 -1.7 518,622 + MPMX20_00503 0.64 -1.4 518,627 + MPMX20_00503 0.65 -0.6 518,630 - MPMX20_00503 0.65 -0.3 518,630 - MPMX20_00503 0.65 -0.8 518,630 - MPMX20_00503 0.65 -1.7 518,630 - MPMX20_00503 0.65 -2.8 518,635 - MPMX20_00503 0.65 -0.7 518,635 - MPMX20_00503 0.65 -0.4 518,657 - MPMX20_00503 0.66 +0.7 518,659 + MPMX20_00503 0.67 -0.6 518,664 + MPMX20_00503 0.67 -0.6 518,664 + MPMX20_00503 0.67 -1.3 518,672 - MPMX20_00503 0.67 -1.5 518,741 + MPMX20_00503 0.71 -2.0 518,749 + MPMX20_00503 0.72 -0.8 518,757 - MPMX20_00503 0.72 -1.3 518,757 - MPMX20_00503 0.72 -0.4 518,774 - MPMX20_00503 0.73 -1.6 518,821 + MPMX20_00503 0.76 -1.5 518,827 - MPMX20_00503 0.76 -1.2 518,863 + MPMX20_00503 0.78 +0.6 518,897 + MPMX20_00503 0.80 -1.8 518,991 + MPMX20_00503 0.86 -0.7 519,023 - MPMX20_00503 0.88 -0.6 519,025 + MPMX20_00503 0.88 -1.3 519,077 + -0.8 519,111 - -1.2 519,139 + -0.6 519,202 - +1.6 519,208 + -2.6 519,216 - +0.3 519,226 - -1.9 519,257 + -0.8 519,265 - -0.5 519,288 + -1.8 519,320 + -1.2 519,328 - -1.2 519,387 - -1.1 519,392 - -1.6 519,505 - -1.1 519,620 - MPMX20_00504 0.10 -0.4 519,644 - MPMX20_00504 0.11 -0.9 519,646 + MPMX20_00504 0.11 -0.8 519,646 + MPMX20_00504 0.11 -1.6 519,649 - MPMX20_00504 0.11 +1.5 519,654 - MPMX20_00504 0.11 +0.4 519,752 + MPMX20_00504 0.14 +0.6 519,757 - MPMX20_00504 0.14 -1.3 519,777 + MPMX20_00504 0.14 -0.1 519,865 - MPMX20_00504 0.17 -0.4 519,942 + MPMX20_00504 0.19 -0.8 519,950 - MPMX20_00504 0.19 -0.7 519,950 - MPMX20_00504 0.19 -1.7 519,950 - MPMX20_00504 0.19 -1.8 519,950 - MPMX20_00504 0.19 +0.6 519,950 - MPMX20_00504 0.19 -1.3 519,950 - MPMX20_00504 0.19 -1.4 519,950 - MPMX20_00504 0.19 -0.1 519,950 - MPMX20_00504 0.19 +1.0 519,950 - MPMX20_00504 0.19 -2.1 519,960 - MPMX20_00504 0.19 -0.4 519,988 + MPMX20_00504 0.20 -0.8 519,988 - MPMX20_00504 0.20 +1.4 519,990 + MPMX20_00504 0.20 -0.6 519,996 - MPMX20_00504 0.20 -0.8 520,002 + MPMX20_00504 0.20 -1.7 520,063 - MPMX20_00504 0.22 -0.6 520,116 + MPMX20_00504 0.23 +1.0 520,118 + MPMX20_00504 0.23 -2.8 520,118 + MPMX20_00504 0.23 -0.8 520,123 + MPMX20_00504 0.24 -1.9 520,123 + MPMX20_00504 0.24 -1.1 520,125 - MPMX20_00504 0.24 -0.9 520,173 + MPMX20_00504 0.25 +0.0 520,181 - MPMX20_00504 0.25 +0.8 520,182 + MPMX20_00504 0.25 -1.6
Or see this region's nucleotide sequence