Experiment: 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00234 and MPMX20_00235 are separated by 194 nucleotides MPMX20_00235 and MPMX20_00236 are separated by 114 nucleotides
MPMX20_00234: MPMX20_00234 - Transcriptional repressor IclR, at 247,312 to 248,139
_00234
MPMX20_00235: MPMX20_00235 - Methionine synthase, at 248,334 to 252,017
_00235
MPMX20_00236: MPMX20_00236 - hypothetical protein, at 252,132 to 253,763
_00236
Position (kb) 248
249
250
251
252
253 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 247.375 kb on - strand at 247.449 kb on + strand, within MPMX20_00234 at 247.449 kb on + strand, within MPMX20_00234 at 247.456 kb on + strand, within MPMX20_00234 at 247.479 kb on + strand, within MPMX20_00234 at 247.479 kb on + strand, within MPMX20_00234 at 247.487 kb on - strand, within MPMX20_00234 at 247.487 kb on - strand, within MPMX20_00234 at 247.487 kb on - strand, within MPMX20_00234 at 247.487 kb on - strand, within MPMX20_00234 at 247.556 kb on + strand, within MPMX20_00234 at 247.564 kb on - strand, within MPMX20_00234 at 247.569 kb on - strand, within MPMX20_00234 at 247.577 kb on + strand, within MPMX20_00234 at 247.585 kb on - strand, within MPMX20_00234 at 247.593 kb on - strand, within MPMX20_00234 at 247.598 kb on - strand, within MPMX20_00234 at 247.600 kb on - strand, within MPMX20_00234 at 247.620 kb on + strand, within MPMX20_00234 at 247.625 kb on + strand, within MPMX20_00234 at 247.625 kb on + strand, within MPMX20_00234 at 247.633 kb on - strand, within MPMX20_00234 at 247.718 kb on + strand, within MPMX20_00234 at 247.726 kb on - strand, within MPMX20_00234 at 247.751 kb on - strand, within MPMX20_00234 at 247.836 kb on + strand, within MPMX20_00234 at 247.836 kb on + strand, within MPMX20_00234 at 247.843 kb on + strand, within MPMX20_00234 at 247.856 kb on - strand, within MPMX20_00234 at 247.866 kb on + strand, within MPMX20_00234 at 247.896 kb on + strand, within MPMX20_00234 at 247.922 kb on + strand, within MPMX20_00234 at 247.958 kb on - strand, within MPMX20_00234 at 248.014 kb on - strand, within MPMX20_00234 at 248.092 kb on + strand at 248.103 kb on + strand at 248.129 kb on + strand at 248.207 kb on - strand at 248.244 kb on - strand at 248.259 kb on + strand at 248.259 kb on + strand at 248.284 kb on + strand at 248.326 kb on + strand at 248.331 kb on + strand at 248.331 kb on + strand at 248.347 kb on - strand at 248.349 kb on + strand at 248.581 kb on + strand at 248.589 kb on - strand at 248.589 kb on - strand at 248.674 kb on + strand at 248.729 kb on + strand, within MPMX20_00235 at 248.797 kb on + strand, within MPMX20_00235 at 248.862 kb on - strand, within MPMX20_00235 at 248.862 kb on - strand, within MPMX20_00235 at 248.899 kb on - strand, within MPMX20_00235 at 248.986 kb on + strand, within MPMX20_00235 at 248.986 kb on + strand, within MPMX20_00235 at 248.994 kb on - strand, within MPMX20_00235 at 249.009 kb on - strand, within MPMX20_00235 at 249.063 kb on + strand, within MPMX20_00235 at 249.066 kb on - strand, within MPMX20_00235 at 249.066 kb on - strand, within MPMX20_00235 at 249.071 kb on - strand, within MPMX20_00235 at 249.082 kb on + strand, within MPMX20_00235 at 249.134 kb on - strand, within MPMX20_00235 at 249.149 kb on + strand, within MPMX20_00235 at 249.180 kb on - strand, within MPMX20_00235 at 249.180 kb on - strand, within MPMX20_00235 at 249.210 kb on + strand, within MPMX20_00235 at 249.240 kb on + strand, within MPMX20_00235 at 249.296 kb on + strand, within MPMX20_00235 at 249.313 kb on - strand, within MPMX20_00235 at 249.364 kb on + strand, within MPMX20_00235 at 249.364 kb on + strand, within MPMX20_00235 at 249.364 kb on + strand, within MPMX20_00235 at 249.364 kb on + strand, within MPMX20_00235 at 249.372 kb on - strand, within MPMX20_00235 at 249.372 kb on - strand, within MPMX20_00235 at 249.372 kb on - strand, within MPMX20_00235 at 249.446 kb on - strand, within MPMX20_00235 at 249.504 kb on + strand, within MPMX20_00235 at 249.540 kb on + strand, within MPMX20_00235 at 249.567 kb on + strand, within MPMX20_00235 at 249.588 kb on - strand, within MPMX20_00235 at 249.678 kb on - strand, within MPMX20_00235 at 249.680 kb on + strand, within MPMX20_00235 at 249.688 kb on - strand, within MPMX20_00235 at 249.749 kb on - strand, within MPMX20_00235 at 249.763 kb on - strand, within MPMX20_00235 at 249.763 kb on - strand, within MPMX20_00235 at 249.806 kb on - strand, within MPMX20_00235 at 249.812 kb on - strand, within MPMX20_00235 at 249.812 kb on - strand, within MPMX20_00235 at 249.812 kb on - strand, within MPMX20_00235 at 249.872 kb on - strand, within MPMX20_00235 at 249.900 kb on - strand, within MPMX20_00235 at 249.925 kb on + strand, within MPMX20_00235 at 249.925 kb on + strand, within MPMX20_00235 at 249.956 kb on - strand, within MPMX20_00235 at 249.985 kb on - strand, within MPMX20_00235 at 250.004 kb on + strand, within MPMX20_00235 at 250.012 kb on - strand, within MPMX20_00235 at 250.058 kb on - strand, within MPMX20_00235 at 250.085 kb on - strand, within MPMX20_00235 at 250.093 kb on - strand, within MPMX20_00235 at 250.093 kb on - strand, within MPMX20_00235 at 250.103 kb on - strand, within MPMX20_00235 at 250.104 kb on + strand, within MPMX20_00235 at 250.112 kb on - strand, within MPMX20_00235 at 250.113 kb on - strand, within MPMX20_00235 at 250.172 kb on - strand, within MPMX20_00235 at 250.175 kb on - strand, within MPMX20_00235 at 250.342 kb on - strand, within MPMX20_00235 at 250.399 kb on + strand, within MPMX20_00235 at 250.417 kb on - strand, within MPMX20_00235 at 250.432 kb on + strand, within MPMX20_00235 at 250.525 kb on + strand, within MPMX20_00235 at 250.528 kb on - strand, within MPMX20_00235 at 250.533 kb on - strand, within MPMX20_00235 at 250.636 kb on + strand, within MPMX20_00235 at 250.644 kb on - strand, within MPMX20_00235 at 250.646 kb on - strand, within MPMX20_00235 at 250.708 kb on - strand, within MPMX20_00235 at 250.726 kb on - strand, within MPMX20_00235 at 250.731 kb on - strand, within MPMX20_00235 at 250.877 kb on + strand, within MPMX20_00235 at 250.877 kb on + strand, within MPMX20_00235 at 250.877 kb on + strand, within MPMX20_00235 at 250.877 kb on + strand, within MPMX20_00235 at 250.877 kb on + strand, within MPMX20_00235 at 250.877 kb on + strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.885 kb on - strand, within MPMX20_00235 at 250.938 kb on + strand, within MPMX20_00235 at 250.989 kb on + strand, within MPMX20_00235 at 250.989 kb on + strand, within MPMX20_00235 at 250.997 kb on - strand, within MPMX20_00235 at 251.013 kb on - strand, within MPMX20_00235 at 251.048 kb on + strand, within MPMX20_00235 at 251.100 kb on - strand, within MPMX20_00235 at 251.100 kb on - strand, within MPMX20_00235 at 251.100 kb on - strand, within MPMX20_00235 at 251.100 kb on - strand, within MPMX20_00235 at 251.100 kb on - strand, within MPMX20_00235 at 251.100 kb on - strand, within MPMX20_00235 at 251.194 kb on + strand, within MPMX20_00235 at 251.202 kb on - strand, within MPMX20_00235 at 251.202 kb on - strand, within MPMX20_00235 at 251.202 kb on - strand, within MPMX20_00235 at 251.214 kb on + strand, within MPMX20_00235 at 251.229 kb on + strand, within MPMX20_00235 at 251.259 kb on + strand, within MPMX20_00235 at 251.269 kb on - strand, within MPMX20_00235 at 251.308 kb on + strand, within MPMX20_00235 at 251.418 kb on - strand, within MPMX20_00235 at 251.418 kb on - strand, within MPMX20_00235 at 251.448 kb on + strand, within MPMX20_00235 at 251.470 kb on + strand, within MPMX20_00235 at 251.619 kb on + strand, within MPMX20_00235 at 251.634 kb on + strand, within MPMX20_00235 at 251.739 kb on + strand at 251.739 kb on + strand at 251.747 kb on - strand at 251.758 kb on + strand at 251.758 kb on + strand at 251.766 kb on - strand at 251.766 kb on - strand at 251.766 kb on - strand at 251.788 kb on + strand at 251.826 kb on - strand at 251.848 kb on + strand at 251.915 kb on + strand at 251.946 kb on - strand at 251.948 kb on + strand at 251.948 kb on + strand at 251.948 kb on + strand at 251.955 kb on + strand at 251.955 kb on + strand at 251.956 kb on - strand at 251.956 kb on - strand at 251.964 kb on - strand at 251.991 kb on - strand at 252.082 kb on + strand at 252.082 kb on + strand at 252.082 kb on + strand at 252.090 kb on - strand at 252.090 kb on - strand at 252.132 kb on + strand at 252.155 kb on + strand at 252.225 kb on + strand at 252.235 kb on - strand at 252.250 kb on + strand at 252.319 kb on + strand, within MPMX20_00236 at 252.348 kb on - strand, within MPMX20_00236 at 252.357 kb on - strand, within MPMX20_00236 at 252.380 kb on + strand, within MPMX20_00236 at 252.429 kb on - strand, within MPMX20_00236 at 252.441 kb on - strand, within MPMX20_00236 at 252.501 kb on - strand, within MPMX20_00236 at 252.526 kb on - strand, within MPMX20_00236 at 252.526 kb on - strand, within MPMX20_00236 at 252.532 kb on + strand, within MPMX20_00236 at 252.544 kb on + strand, within MPMX20_00236 at 252.599 kb on + strand, within MPMX20_00236 at 252.607 kb on - strand, within MPMX20_00236 at 252.607 kb on - strand, within MPMX20_00236 at 252.669 kb on + strand, within MPMX20_00236 at 252.677 kb on - strand, within MPMX20_00236 at 252.688 kb on + strand, within MPMX20_00236 at 252.771 kb on - strand, within MPMX20_00236 at 252.783 kb on - strand, within MPMX20_00236 at 252.809 kb on + strand, within MPMX20_00236 at 252.920 kb on + strand, within MPMX20_00236 at 252.946 kb on - strand, within MPMX20_00236 at 252.946 kb on - strand, within MPMX20_00236 at 252.951 kb on - strand, within MPMX20_00236 at 252.991 kb on + strand, within MPMX20_00236 at 253.017 kb on + strand, within MPMX20_00236 at 253.017 kb on + strand, within MPMX20_00236
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 247,375 - +0.7 247,449 + MPMX20_00234 0.17 -0.7 247,449 + MPMX20_00234 0.17 -1.3 247,456 + MPMX20_00234 0.17 +2.7 247,479 + MPMX20_00234 0.20 +1.1 247,479 + MPMX20_00234 0.20 -0.1 247,487 - MPMX20_00234 0.21 -0.1 247,487 - MPMX20_00234 0.21 -0.3 247,487 - MPMX20_00234 0.21 -2.6 247,487 - MPMX20_00234 0.21 -0.8 247,556 + MPMX20_00234 0.29 +0.4 247,564 - MPMX20_00234 0.30 +0.3 247,569 - MPMX20_00234 0.31 +1.1 247,577 + MPMX20_00234 0.32 -1.1 247,585 - MPMX20_00234 0.33 -1.3 247,593 - MPMX20_00234 0.34 -1.7 247,598 - MPMX20_00234 0.35 +1.1 247,600 - MPMX20_00234 0.35 -1.1 247,620 + MPMX20_00234 0.37 -1.9 247,625 + MPMX20_00234 0.38 -1.4 247,625 + MPMX20_00234 0.38 -1.9 247,633 - MPMX20_00234 0.39 -2.3 247,718 + MPMX20_00234 0.49 -0.6 247,726 - MPMX20_00234 0.50 -0.8 247,751 - MPMX20_00234 0.53 +0.5 247,836 + MPMX20_00234 0.63 -0.7 247,836 + MPMX20_00234 0.63 -2.0 247,843 + MPMX20_00234 0.64 -1.4 247,856 - MPMX20_00234 0.66 -0.7 247,866 + MPMX20_00234 0.67 +0.7 247,896 + MPMX20_00234 0.71 -1.2 247,922 + MPMX20_00234 0.74 -2.3 247,958 - MPMX20_00234 0.78 +0.9 248,014 - MPMX20_00234 0.85 -0.3 248,092 + +1.5 248,103 + -1.3 248,129 + -2.2 248,207 - -0.3 248,244 - +0.9 248,259 + +0.7 248,259 + -1.5 248,284 + +2.7 248,326 + -1.2 248,331 + +1.1 248,331 + +0.1 248,347 - -1.0 248,349 + -0.8 248,581 + -0.6 248,589 - +0.5 248,589 - +0.3 248,674 + +2.7 248,729 + MPMX20_00235 0.11 +0.0 248,797 + MPMX20_00235 0.13 +1.1 248,862 - MPMX20_00235 0.14 +0.4 248,862 - MPMX20_00235 0.14 -0.1 248,899 - MPMX20_00235 0.15 -1.7 248,986 + MPMX20_00235 0.18 -0.9 248,986 + MPMX20_00235 0.18 +0.0 248,994 - MPMX20_00235 0.18 -0.3 249,009 - MPMX20_00235 0.18 +0.0 249,063 + MPMX20_00235 0.20 -1.9 249,066 - MPMX20_00235 0.20 +0.3 249,066 - MPMX20_00235 0.20 -2.1 249,071 - MPMX20_00235 0.20 +1.1 249,082 + MPMX20_00235 0.20 +1.7 249,134 - MPMX20_00235 0.22 -0.0 249,149 + MPMX20_00235 0.22 -0.3 249,180 - MPMX20_00235 0.23 +1.5 249,180 - MPMX20_00235 0.23 +0.9 249,210 + MPMX20_00235 0.24 +0.1 249,240 + MPMX20_00235 0.25 -0.6 249,296 + MPMX20_00235 0.26 -2.6 249,313 - MPMX20_00235 0.27 -2.2 249,364 + MPMX20_00235 0.28 -1.1 249,364 + MPMX20_00235 0.28 -0.3 249,364 + MPMX20_00235 0.28 -1.0 249,364 + MPMX20_00235 0.28 +0.6 249,372 - MPMX20_00235 0.28 -1.1 249,372 - MPMX20_00235 0.28 -0.9 249,372 - MPMX20_00235 0.28 +2.7 249,446 - MPMX20_00235 0.30 +1.4 249,504 + MPMX20_00235 0.32 -0.7 249,540 + MPMX20_00235 0.33 +0.9 249,567 + MPMX20_00235 0.33 -1.0 249,588 - MPMX20_00235 0.34 -0.7 249,678 - MPMX20_00235 0.36 -0.3 249,680 + MPMX20_00235 0.37 +0.7 249,688 - MPMX20_00235 0.37 -0.6 249,749 - MPMX20_00235 0.38 +2.7 249,763 - MPMX20_00235 0.39 -1.0 249,763 - MPMX20_00235 0.39 +0.4 249,806 - MPMX20_00235 0.40 -0.2 249,812 - MPMX20_00235 0.40 +2.1 249,812 - MPMX20_00235 0.40 +1.6 249,812 - MPMX20_00235 0.40 +0.9 249,872 - MPMX20_00235 0.42 -1.0 249,900 - MPMX20_00235 0.43 -2.2 249,925 + MPMX20_00235 0.43 -1.6 249,925 + MPMX20_00235 0.43 +1.5 249,956 - MPMX20_00235 0.44 -0.7 249,985 - MPMX20_00235 0.45 -0.1 250,004 + MPMX20_00235 0.45 +0.4 250,012 - MPMX20_00235 0.46 +0.7 250,058 - MPMX20_00235 0.47 -0.3 250,085 - MPMX20_00235 0.48 -1.0 250,093 - MPMX20_00235 0.48 -0.3 250,093 - MPMX20_00235 0.48 -1.2 250,103 - MPMX20_00235 0.48 +1.7 250,104 + MPMX20_00235 0.48 +0.4 250,112 - MPMX20_00235 0.48 -0.1 250,113 - MPMX20_00235 0.48 +0.9 250,172 - MPMX20_00235 0.50 -1.0 250,175 - MPMX20_00235 0.50 -2.1 250,342 - MPMX20_00235 0.55 -1.0 250,399 + MPMX20_00235 0.56 -0.1 250,417 - MPMX20_00235 0.57 +2.7 250,432 + MPMX20_00235 0.57 -0.5 250,525 + MPMX20_00235 0.59 +1.1 250,528 - MPMX20_00235 0.60 +0.5 250,533 - MPMX20_00235 0.60 -0.1 250,636 + MPMX20_00235 0.62 -0.4 250,644 - MPMX20_00235 0.63 -0.5 250,646 - MPMX20_00235 0.63 +0.4 250,708 - MPMX20_00235 0.64 +0.8 250,726 - MPMX20_00235 0.65 -1.1 250,731 - MPMX20_00235 0.65 -0.7 250,877 + MPMX20_00235 0.69 -0.8 250,877 + MPMX20_00235 0.69 -0.5 250,877 + MPMX20_00235 0.69 -1.4 250,877 + MPMX20_00235 0.69 +0.4 250,877 + MPMX20_00235 0.69 -0.5 250,877 + MPMX20_00235 0.69 -0.9 250,885 - MPMX20_00235 0.69 +0.7 250,885 - MPMX20_00235 0.69 +0.4 250,885 - MPMX20_00235 0.69 +1.1 250,885 - MPMX20_00235 0.69 +1.5 250,885 - MPMX20_00235 0.69 -1.2 250,885 - MPMX20_00235 0.69 +2.7 250,885 - MPMX20_00235 0.69 +1.7 250,885 - MPMX20_00235 0.69 +0.5 250,938 + MPMX20_00235 0.71 +2.7 250,989 + MPMX20_00235 0.72 +1.1 250,989 + MPMX20_00235 0.72 +0.7 250,997 - MPMX20_00235 0.72 +1.7 251,013 - MPMX20_00235 0.73 -0.4 251,048 + MPMX20_00235 0.74 -1.7 251,100 - MPMX20_00235 0.75 -1.6 251,100 - MPMX20_00235 0.75 +0.6 251,100 - MPMX20_00235 0.75 -1.7 251,100 - MPMX20_00235 0.75 -0.3 251,100 - MPMX20_00235 0.75 -1.5 251,100 - MPMX20_00235 0.75 -1.7 251,194 + MPMX20_00235 0.78 -0.1 251,202 - MPMX20_00235 0.78 +0.0 251,202 - MPMX20_00235 0.78 -0.6 251,202 - MPMX20_00235 0.78 -0.8 251,214 + MPMX20_00235 0.78 -2.1 251,229 + MPMX20_00235 0.79 +5.7 251,259 + MPMX20_00235 0.79 -2.0 251,269 - MPMX20_00235 0.80 -1.5 251,308 + MPMX20_00235 0.81 +0.8 251,418 - MPMX20_00235 0.84 -1.6 251,418 - MPMX20_00235 0.84 -1.0 251,448 + MPMX20_00235 0.85 -0.3 251,470 + MPMX20_00235 0.85 -0.7 251,619 + MPMX20_00235 0.89 +1.7 251,634 + MPMX20_00235 0.90 +0.3 251,739 + +1.1 251,739 + -1.7 251,747 - -1.1 251,758 + +0.9 251,758 + +1.1 251,766 - +0.7 251,766 - -0.3 251,766 - -0.2 251,788 + -0.6 251,826 - +0.1 251,848 + -0.8 251,915 + -0.1 251,946 - +1.1 251,948 + -1.8 251,948 + -0.5 251,948 + +2.1 251,955 + -2.0 251,955 + -1.2 251,956 - -0.4 251,956 - -2.1 251,964 - -0.4 251,991 - +0.1 252,082 + -0.2 252,082 + +0.2 252,082 + +0.1 252,090 - -0.9 252,090 - -1.1 252,132 + -0.4 252,155 + +1.7 252,225 + -0.3 252,235 - -1.5 252,250 + -1.1 252,319 + MPMX20_00236 0.11 +1.0 252,348 - MPMX20_00236 0.13 -0.1 252,357 - MPMX20_00236 0.14 -0.4 252,380 + MPMX20_00236 0.15 -0.1 252,429 - MPMX20_00236 0.18 -2.2 252,441 - MPMX20_00236 0.19 +0.4 252,501 - MPMX20_00236 0.23 -2.6 252,526 - MPMX20_00236 0.24 -0.6 252,526 - MPMX20_00236 0.24 +0.6 252,532 + MPMX20_00236 0.25 +0.4 252,544 + MPMX20_00236 0.25 -2.5 252,599 + MPMX20_00236 0.29 -1.6 252,607 - MPMX20_00236 0.29 -0.9 252,607 - MPMX20_00236 0.29 -0.3 252,669 + MPMX20_00236 0.33 +0.4 252,677 - MPMX20_00236 0.33 -1.9 252,688 + MPMX20_00236 0.34 -0.9 252,771 - MPMX20_00236 0.39 +0.7 252,783 - MPMX20_00236 0.40 -1.6 252,809 + MPMX20_00236 0.41 -0.8 252,920 + MPMX20_00236 0.48 +0.6 252,946 - MPMX20_00236 0.50 -0.9 252,946 - MPMX20_00236 0.50 +0.7 252,951 - MPMX20_00236 0.50 -2.6 252,991 + MPMX20_00236 0.53 -0.3 253,017 + MPMX20_00236 0.54 -0.6 253,017 + MPMX20_00236 0.54 -0.3
Or see this region's nucleotide sequence