Experiment: 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00017 and MPMX20_00018 overlap by 33 nucleotides MPMX20_00018 and MPMX20_00019 are separated by 223 nucleotides MPMX20_00019 and MPMX20_00020 overlap by 4 nucleotides
MPMX20_00017: MPMX20_00017 - Aspartate/alanine antiporter, at 17,205 to 18,866
_00017
MPMX20_00018: MPMX20_00018 - Mannosyl-D-glycerate transport/metabolism system repressor MngR, at 18,834 to 19,580
_00018
MPMX20_00019: MPMX20_00019 - hypothetical protein, at 19,804 to 21,426
_00019
MPMX20_00020: MPMX20_00020 - 6-phospho-alpha-glucosidase, at 21,423 to 22,745
_00020
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.820 kb on + strand at 18.828 kb on - strand at 18.828 kb on - strand at 18.833 kb on + strand at 18.848 kb on + strand at 18.854 kb on + strand at 19.016 kb on + strand, within MPMX20_00018 at 19.065 kb on + strand, within MPMX20_00018 at 19.119 kb on + strand, within MPMX20_00018 at 19.127 kb on - strand, within MPMX20_00018 at 19.151 kb on + strand, within MPMX20_00018 at 19.178 kb on - strand, within MPMX20_00018 at 19.254 kb on + strand, within MPMX20_00018 at 19.290 kb on - strand, within MPMX20_00018 at 19.302 kb on + strand, within MPMX20_00018 at 19.312 kb on + strand, within MPMX20_00018 at 19.333 kb on + strand, within MPMX20_00018 at 19.337 kb on + strand, within MPMX20_00018 at 19.419 kb on - strand, within MPMX20_00018 at 19.493 kb on + strand, within MPMX20_00018 at 19.547 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.599 kb on + strand at 19.607 kb on - strand at 19.607 kb on - strand at 19.607 kb on - strand at 19.607 kb on - strand at 19.607 kb on - strand at 19.607 kb on - strand at 19.616 kb on - strand at 19.658 kb on - strand at 19.728 kb on - strand at 19.733 kb on + strand at 19.738 kb on + strand at 19.746 kb on - strand at 19.766 kb on + strand at 19.774 kb on - strand at 19.782 kb on - strand at 19.790 kb on - strand at 19.811 kb on + strand at 19.847 kb on - strand at 19.852 kb on - strand at 19.889 kb on + strand at 19.897 kb on - strand at 19.924 kb on + strand at 19.924 kb on + strand at 19.945 kb on - strand at 19.960 kb on + strand at 19.967 kb on + strand, within MPMX20_00019 at 19.969 kb on + strand, within MPMX20_00019 at 20.074 kb on + strand, within MPMX20_00019 at 20.074 kb on - strand, within MPMX20_00019 at 20.076 kb on + strand, within MPMX20_00019 at 20.118 kb on + strand, within MPMX20_00019 at 20.260 kb on + strand, within MPMX20_00019 at 20.260 kb on + strand, within MPMX20_00019 at 20.283 kb on + strand, within MPMX20_00019 at 20.454 kb on + strand, within MPMX20_00019 at 20.455 kb on + strand, within MPMX20_00019 at 20.463 kb on - strand, within MPMX20_00019 at 20.463 kb on - strand, within MPMX20_00019 at 20.523 kb on - strand, within MPMX20_00019 at 20.547 kb on - strand, within MPMX20_00019 at 20.650 kb on + strand, within MPMX20_00019 at 20.656 kb on + strand, within MPMX20_00019 at 20.656 kb on + strand, within MPMX20_00019 at 20.749 kb on - strand, within MPMX20_00019 at 20.974 kb on + strand, within MPMX20_00019 at 20.981 kb on + strand, within MPMX20_00019 at 21.003 kb on - strand, within MPMX20_00019 at 21.012 kb on + strand, within MPMX20_00019 at 21.031 kb on + strand, within MPMX20_00019 at 21.047 kb on - strand, within MPMX20_00019 at 21.047 kb on - strand, within MPMX20_00019 at 21.047 kb on - strand, within MPMX20_00019 at 21.152 kb on - strand, within MPMX20_00019 at 21.277 kb on + strand at 21.293 kb on + strand at 21.301 kb on - strand at 21.334 kb on - strand at 21.341 kb on + strand at 21.341 kb on + strand at 21.349 kb on - strand at 21.368 kb on - strand at 21.463 kb on + strand at 21.552 kb on + strand at 21.552 kb on + strand at 21.552 kb on + strand at 21.554 kb on + strand at 21.560 kb on - strand, within MPMX20_00020 at 21.560 kb on - strand, within MPMX20_00020 at 21.560 kb on - strand, within MPMX20_00020 at 21.565 kb on + strand, within MPMX20_00020 at 21.573 kb on - strand, within MPMX20_00020 at 21.586 kb on + strand, within MPMX20_00020 at 21.594 kb on - strand, within MPMX20_00020 at 21.631 kb on + strand, within MPMX20_00020 at 21.632 kb on + strand, within MPMX20_00020 at 21.642 kb on + strand, within MPMX20_00020 at 21.642 kb on + strand, within MPMX20_00020 at 21.650 kb on - strand, within MPMX20_00020 at 21.665 kb on - strand, within MPMX20_00020 at 21.669 kb on + strand, within MPMX20_00020 at 21.677 kb on - strand, within MPMX20_00020 at 21.677 kb on - strand, within MPMX20_00020 at 21.694 kb on - strand, within MPMX20_00020 at 21.733 kb on - strand, within MPMX20_00020 at 21.735 kb on + strand, within MPMX20_00020 at 21.735 kb on + strand, within MPMX20_00020 at 21.822 kb on + strand, within MPMX20_00020 at 21.830 kb on - strand, within MPMX20_00020 at 21.841 kb on + strand, within MPMX20_00020 at 21.856 kb on + strand, within MPMX20_00020 at 21.864 kb on - strand, within MPMX20_00020 at 21.865 kb on - strand, within MPMX20_00020 at 21.973 kb on + strand, within MPMX20_00020 at 21.981 kb on - strand, within MPMX20_00020 at 21.981 kb on - strand, within MPMX20_00020 at 21.990 kb on - strand, within MPMX20_00020 at 22.006 kb on + strand, within MPMX20_00020 at 22.185 kb on + strand, within MPMX20_00020 at 22.250 kb on + strand, within MPMX20_00020 at 22.302 kb on + strand, within MPMX20_00020 at 22.307 kb on + strand, within MPMX20_00020 at 22.307 kb on + strand, within MPMX20_00020 at 22.315 kb on - strand, within MPMX20_00020 at 22.366 kb on - strand, within MPMX20_00020 at 22.418 kb on - strand, within MPMX20_00020
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, Mixed culture; Streptomyces sp. SAI_117 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 18,820 + +0.9 18,828 - +0.4 18,828 - +1.8 18,833 + -0.5 18,848 + -0.6 18,854 + -0.5 19,016 + MPMX20_00018 0.24 +1.2 19,065 + MPMX20_00018 0.31 -2.0 19,119 + MPMX20_00018 0.38 -1.6 19,127 - MPMX20_00018 0.39 -0.3 19,151 + MPMX20_00018 0.42 -0.3 19,178 - MPMX20_00018 0.46 -0.5 19,254 + MPMX20_00018 0.56 -0.0 19,290 - MPMX20_00018 0.61 -0.6 19,302 + MPMX20_00018 0.63 -0.9 19,312 + MPMX20_00018 0.64 +0.5 19,333 + MPMX20_00018 0.67 +0.7 19,337 + MPMX20_00018 0.67 +0.4 19,419 - MPMX20_00018 0.78 -1.2 19,493 + MPMX20_00018 0.88 -0.1 19,547 + +0.2 19,599 + +0.3 19,599 + +0.4 19,599 + -0.4 19,599 + -2.8 19,599 + -1.5 19,599 + +1.7 19,599 + -0.8 19,599 + +0.7 19,599 + -0.8 19,599 + -0.3 19,607 - -0.6 19,607 - -1.2 19,607 - -0.0 19,607 - -1.5 19,607 - -2.5 19,607 - -2.6 19,616 - +0.3 19,658 - -1.1 19,728 - -1.0 19,733 + -0.8 19,738 + -0.8 19,746 - -1.5 19,766 + -0.6 19,774 - +1.1 19,782 - +0.2 19,790 - -1.2 19,811 + +0.4 19,847 - -1.1 19,852 - -0.4 19,889 + -0.9 19,897 - -1.7 19,924 + +0.1 19,924 + -1.1 19,945 - -1.0 19,960 + -0.7 19,967 + MPMX20_00019 0.10 -1.5 19,969 + MPMX20_00019 0.10 -1.7 20,074 + MPMX20_00019 0.17 +0.7 20,074 - MPMX20_00019 0.17 +1.1 20,076 + MPMX20_00019 0.17 -2.0 20,118 + MPMX20_00019 0.19 +0.2 20,260 + MPMX20_00019 0.28 -0.3 20,260 + MPMX20_00019 0.28 -2.3 20,283 + MPMX20_00019 0.30 +0.8 20,454 + MPMX20_00019 0.40 -1.7 20,455 + MPMX20_00019 0.40 -0.6 20,463 - MPMX20_00019 0.41 -0.1 20,463 - MPMX20_00019 0.41 -2.1 20,523 - MPMX20_00019 0.44 +1.4 20,547 - MPMX20_00019 0.46 -0.3 20,650 + MPMX20_00019 0.52 -2.3 20,656 + MPMX20_00019 0.52 -1.5 20,656 + MPMX20_00019 0.52 +0.5 20,749 - MPMX20_00019 0.58 +0.4 20,974 + MPMX20_00019 0.72 +0.5 20,981 + MPMX20_00019 0.73 -1.4 21,003 - MPMX20_00019 0.74 -1.5 21,012 + MPMX20_00019 0.74 +1.7 21,031 + MPMX20_00019 0.76 -1.1 21,047 - MPMX20_00019 0.77 +0.2 21,047 - MPMX20_00019 0.77 -0.4 21,047 - MPMX20_00019 0.77 -0.3 21,152 - MPMX20_00019 0.83 +1.7 21,277 + -1.8 21,293 + -0.5 21,301 - -0.8 21,334 - -2.6 21,341 + -1.6 21,341 + -2.6 21,349 - -0.2 21,368 - +0.0 21,463 + -0.0 21,552 + -0.3 21,552 + -0.1 21,552 + -0.6 21,554 + -1.6 21,560 - MPMX20_00020 0.10 -0.6 21,560 - MPMX20_00020 0.10 -0.4 21,560 - MPMX20_00020 0.10 -0.8 21,565 + MPMX20_00020 0.11 -0.6 21,573 - MPMX20_00020 0.11 +2.7 21,586 + MPMX20_00020 0.12 -1.7 21,594 - MPMX20_00020 0.13 +0.2 21,631 + MPMX20_00020 0.16 -1.2 21,632 + MPMX20_00020 0.16 +0.0 21,642 + MPMX20_00020 0.17 +0.4 21,642 + MPMX20_00020 0.17 -1.4 21,650 - MPMX20_00020 0.17 -0.9 21,665 - MPMX20_00020 0.18 +0.7 21,669 + MPMX20_00020 0.19 +0.4 21,677 - MPMX20_00020 0.19 +0.1 21,677 - MPMX20_00020 0.19 -0.4 21,694 - MPMX20_00020 0.20 +1.7 21,733 - MPMX20_00020 0.23 -1.8 21,735 + MPMX20_00020 0.24 -0.1 21,735 + MPMX20_00020 0.24 -1.0 21,822 + MPMX20_00020 0.30 -1.4 21,830 - MPMX20_00020 0.31 +1.7 21,841 + MPMX20_00020 0.32 -0.3 21,856 + MPMX20_00020 0.33 -0.8 21,864 - MPMX20_00020 0.33 -0.7 21,865 - MPMX20_00020 0.33 +1.1 21,973 + MPMX20_00020 0.42 +2.7 21,981 - MPMX20_00020 0.42 -1.0 21,981 - MPMX20_00020 0.42 -1.4 21,990 - MPMX20_00020 0.43 -0.2 22,006 + MPMX20_00020 0.44 -1.1 22,185 + MPMX20_00020 0.58 -0.5 22,250 + MPMX20_00020 0.63 +0.1 22,302 + MPMX20_00020 0.66 +0.9 22,307 + MPMX20_00020 0.67 -0.5 22,307 + MPMX20_00020 0.67 +0.2 22,315 - MPMX20_00020 0.67 +0.1 22,366 - MPMX20_00020 0.71 -1.9 22,418 - MPMX20_00020 0.75 +1.7
Or see this region's nucleotide sequence