Experiment: 0.1X LB plate, Mixed culture; Streptomyces albogriseolus SAI_072 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00774 and MPMX20_00775 are separated by 2 nucleotides MPMX20_00775 and MPMX20_00776 overlap by 4 nucleotides MPMX20_00776 and MPMX20_00777 are separated by 15 nucleotides
MPMX20_00774: MPMX20_00774 - Transcriptional regulator MraZ, at 808,671 to 809,165
_00774
MPMX20_00775: MPMX20_00775 - Ribosomal RNA small subunit methyltransferase H, at 809,168 to 810,109
_00775
MPMX20_00776: MPMX20_00776 - Cell division protein FtsL, at 810,106 to 810,471
_00776
MPMX20_00777: MPMX20_00777 - Peptidoglycan D,D-transpeptidase FtsI, at 810,487 to 812,253
_00777
Position (kb) 809
810
811 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 808.231 kb on + strand at 808.239 kb on - strand at 808.250 kb on - strand at 808.251 kb on - strand at 808.261 kb on - strand at 808.308 kb on - strand at 808.308 kb on - strand at 808.318 kb on - strand at 808.327 kb on - strand at 808.340 kb on - strand at 808.340 kb on - strand at 808.340 kb on - strand at 808.342 kb on + strand at 808.342 kb on + strand at 808.342 kb on + strand at 808.342 kb on + strand at 808.342 kb on + strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.350 kb on - strand at 808.361 kb on - strand at 808.377 kb on + strand at 808.377 kb on + strand at 808.377 kb on + strand at 808.377 kb on + strand at 808.377 kb on + strand at 808.377 kb on + strand at 808.382 kb on + strand at 808.382 kb on + strand at 808.382 kb on + strand at 808.383 kb on + strand at 808.383 kb on + strand at 808.384 kb on + strand at 808.385 kb on - strand at 808.385 kb on - strand at 808.385 kb on - strand at 808.385 kb on - strand at 808.385 kb on - strand at 808.385 kb on - strand at 808.386 kb on - strand at 808.388 kb on + strand at 808.391 kb on - strand at 808.397 kb on + strand at 808.397 kb on + strand at 808.397 kb on - strand at 808.397 kb on - strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.399 kb on + strand at 808.404 kb on + strand at 808.404 kb on - strand at 808.405 kb on - strand at 808.406 kb on + strand at 808.407 kb on - strand at 808.407 kb on - strand at 808.407 kb on - strand at 808.407 kb on - strand at 808.407 kb on - strand at 808.424 kb on + strand at 808.442 kb on + strand at 808.450 kb on + strand at 808.454 kb on + strand at 808.469 kb on + strand at 808.477 kb on - strand at 808.477 kb on - strand at 808.477 kb on - strand at 808.479 kb on - strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.481 kb on + strand at 808.489 kb on - strand at 808.489 kb on - strand at 808.489 kb on - strand at 808.490 kb on - strand at 808.492 kb on + strand at 808.495 kb on - strand at 808.500 kb on - strand at 808.500 kb on - strand at 808.500 kb on - strand at 808.550 kb on + strand at 808.558 kb on + strand at 808.558 kb on - strand at 808.564 kb on + strand at 808.564 kb on + strand at 808.566 kb on - strand at 808.572 kb on + strand at 808.572 kb on + strand at 808.572 kb on + strand at 808.572 kb on - strand at 808.572 kb on - strand at 808.572 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.580 kb on - strand at 808.582 kb on - strand at 808.582 kb on - strand at 808.582 kb on - strand at 808.589 kb on - strand at 808.595 kb on + strand at 808.596 kb on - strand at 808.597 kb on + strand at 808.602 kb on + strand at 808.602 kb on + strand at 808.604 kb on - strand at 808.605 kb on - strand at 808.605 kb on - strand at 808.605 kb on - strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.607 kb on + strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.610 kb on - strand at 808.614 kb on - strand at 808.614 kb on - strand at 808.614 kb on - strand at 808.615 kb on - strand at 808.615 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.616 kb on - strand at 808.617 kb on + strand at 808.617 kb on + strand at 808.617 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.618 kb on + strand at 808.621 kb on - strand at 808.621 kb on - strand at 808.621 kb on - strand at 808.625 kb on - strand at 808.625 kb on - strand at 808.625 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.626 kb on - strand at 808.631 kb on - strand at 808.683 kb on + strand at 808.685 kb on + strand at 808.690 kb on + strand at 808.694 kb on + strand at 808.695 kb on + strand at 808.704 kb on + strand at 808.704 kb on + strand at 808.704 kb on + strand at 808.712 kb on + strand at 808.717 kb on + strand at 808.717 kb on + strand at 808.764 kb on + strand, within MPMX20_00774 at 808.764 kb on + strand, within MPMX20_00774 at 808.831 kb on + strand, within MPMX20_00774 at 808.839 kb on + strand, within MPMX20_00774 at 808.844 kb on + strand, within MPMX20_00774 at 808.844 kb on + strand, within MPMX20_00774 at 808.844 kb on + strand, within MPMX20_00774 at 808.844 kb on + strand, within MPMX20_00774 at 808.844 kb on + strand, within MPMX20_00774 at 808.969 kb on + strand, within MPMX20_00774 at 808.989 kb on + strand, within MPMX20_00774 at 809.013 kb on + strand, within MPMX20_00774 at 809.045 kb on + strand, within MPMX20_00774 at 809.053 kb on + strand, within MPMX20_00774 at 809.053 kb on + strand, within MPMX20_00774 at 809.061 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.080 kb on + strand, within MPMX20_00774 at 809.090 kb on + strand, within MPMX20_00774 at 809.090 kb on + strand, within MPMX20_00774 at 809.107 kb on + strand, within MPMX20_00774 at 809.107 kb on + strand, within MPMX20_00774 at 809.160 kb on + strand at 809.320 kb on + strand, within MPMX20_00775 at 809.364 kb on + strand, within MPMX20_00775 at 809.481 kb on + strand, within MPMX20_00775 at 809.569 kb on + strand, within MPMX20_00775 at 809.620 kb on + strand, within MPMX20_00775 at 809.711 kb on + strand, within MPMX20_00775 at 809.789 kb on + strand, within MPMX20_00775 at 810.072 kb on + strand at 810.486 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces albogriseolus SAI_072 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 808,231 + -0.3 808,239 - +0.2 808,250 - +0.2 808,251 - +0.3 808,261 - +3.3 808,308 - -0.3 808,308 - -0.2 808,318 - -1.7 808,327 - -0.5 808,340 - -0.4 808,340 - +0.2 808,340 - +0.9 808,342 + -0.8 808,342 + -0.7 808,342 + -0.7 808,342 + -0.6 808,342 + +1.3 808,350 - -0.7 808,350 - -0.1 808,350 - -1.3 808,350 - +0.6 808,350 - -0.1 808,350 - -0.8 808,350 - -0.5 808,350 - -2.0 808,350 - -1.0 808,350 - -0.3 808,361 - -0.9 808,377 + -0.3 808,377 + -0.4 808,377 + +0.6 808,377 + -1.1 808,377 + -0.9 808,377 + -0.4 808,382 + -0.4 808,382 + +0.6 808,382 + +0.9 808,383 + +0.7 808,383 + -0.0 808,384 + -0.8 808,385 - +1.1 808,385 - +0.2 808,385 - +0.1 808,385 - -0.6 808,385 - -0.3 808,385 - +0.3 808,386 - -0.0 808,388 + -1.3 808,391 - -0.2 808,397 + -0.1 808,397 + -1.3 808,397 - -2.2 808,397 - +1.5 808,399 + -2.9 808,399 + -0.1 808,399 + -0.2 808,399 + -0.9 808,399 + -0.4 808,399 + -0.1 808,399 + +1.4 808,399 + -1.4 808,399 + -1.6 808,399 + +1.3 808,399 + +0.1 808,399 + +2.6 808,399 + +0.2 808,399 + +0.6 808,399 + -0.0 808,399 + -0.1 808,399 + +0.3 808,404 + -0.9 808,404 - -1.1 808,405 - +0.5 808,406 + +2.1 808,407 - +0.1 808,407 - +0.9 808,407 - -0.9 808,407 - +1.9 808,407 - -0.2 808,424 + -0.5 808,442 + -0.4 808,450 + -0.4 808,454 + +1.3 808,469 + +2.9 808,477 - +0.9 808,477 - +1.5 808,477 - +0.3 808,479 - -1.4 808,481 + +0.5 808,481 + -0.1 808,481 + -1.3 808,481 + -0.1 808,481 + -2.0 808,481 + -0.4 808,481 + +0.5 808,481 + +0.5 808,489 - -1.8 808,489 - -0.1 808,489 - +0.2 808,490 - +0.3 808,492 + +0.3 808,495 - -0.5 808,500 - -2.3 808,500 - +2.9 808,500 - -1.9 808,550 + -0.4 808,558 + -0.3 808,558 - -1.1 808,564 + -0.0 808,564 + -0.5 808,566 - +1.4 808,572 + +0.2 808,572 + -0.9 808,572 + +0.8 808,572 - -2.1 808,572 - -0.2 808,572 - -0.3 808,580 - -0.1 808,580 - +1.9 808,580 - +0.3 808,580 - -0.1 808,580 - +1.2 808,580 - -0.0 808,580 - +0.7 808,580 - -0.7 808,582 - +0.1 808,582 - +2.4 808,582 - +1.9 808,589 - +0.2 808,595 + -1.4 808,596 - -0.4 808,597 + +1.1 808,602 + -1.1 808,602 + -0.0 808,604 - -0.7 808,605 - +1.5 808,605 - -0.4 808,605 - -0.4 808,607 + -1.4 808,607 + -1.9 808,607 + +0.7 808,607 + +0.6 808,607 + +1.1 808,607 + -0.2 808,607 + -1.2 808,607 + +0.8 808,607 + +0.3 808,610 - -0.7 808,610 - +0.0 808,610 - -1.2 808,610 - +0.9 808,610 - +0.3 808,610 - +1.0 808,610 - +0.9 808,610 - -2.0 808,610 - +0.4 808,610 - +2.8 808,610 - +0.9 808,610 - -0.2 808,614 - -0.1 808,614 - -1.2 808,614 - -1.7 808,615 - +0.2 808,615 - +0.1 808,616 - -0.8 808,616 - +0.1 808,616 - -0.3 808,616 - +0.8 808,616 - +0.5 808,616 - +1.0 808,616 - -1.0 808,616 - +0.7 808,616 - +0.2 808,616 - -0.2 808,616 - -0.4 808,616 - +0.0 808,616 - +0.9 808,616 - -1.3 808,616 - +0.1 808,616 - -1.0 808,616 - +0.3 808,616 - -0.1 808,616 - -1.8 808,617 + +1.2 808,617 + +1.9 808,617 + +0.2 808,618 + +0.9 808,618 + -0.7 808,618 + +0.5 808,618 + -0.0 808,618 + +0.6 808,618 + -0.4 808,618 + +1.6 808,618 + +1.5 808,618 + -0.4 808,618 + -0.2 808,618 + +0.9 808,618 + -0.4 808,618 + -0.4 808,618 + -1.8 808,618 + -0.2 808,618 + -1.0 808,618 + -0.3 808,618 + -0.8 808,618 + -1.3 808,618 + -0.8 808,618 + -0.4 808,618 + -3.5 808,618 + -0.3 808,618 + +0.5 808,618 + -1.9 808,618 + -1.2 808,618 + -0.5 808,618 + -1.2 808,618 + +0.7 808,618 + -0.1 808,618 + -0.3 808,618 + +0.3 808,618 + -0.9 808,618 + +1.9 808,618 + +0.4 808,621 - -0.4 808,621 - +0.9 808,621 - +1.0 808,625 - +0.3 808,625 - -0.3 808,625 - -0.3 808,626 - -1.9 808,626 - -1.1 808,626 - +0.1 808,626 - -1.3 808,626 - -0.1 808,626 - +0.4 808,626 - -1.5 808,626 - -0.1 808,626 - +0.8 808,626 - -0.2 808,626 - -0.0 808,626 - +1.4 808,626 - -0.5 808,626 - -0.6 808,626 - -0.6 808,626 - -0.4 808,626 - -2.7 808,626 - -1.4 808,626 - -0.9 808,626 - -0.1 808,626 - +0.0 808,626 - +0.9 808,626 - +0.3 808,626 - -0.4 808,626 - -0.9 808,626 - +0.4 808,626 - +0.8 808,626 - -1.3 808,626 - +0.5 808,626 - +0.8 808,626 - -0.4 808,626 - -0.7 808,626 - -1.2 808,626 - -2.1 808,626 - -0.3 808,626 - +0.1 808,626 - +1.5 808,626 - -0.6 808,626 - +1.0 808,626 - -1.0 808,626 - +0.1 808,626 - -0.7 808,626 - -0.6 808,626 - -1.5 808,626 - +0.4 808,626 - -1.1 808,626 - -2.7 808,626 - +0.2 808,626 - -0.7 808,626 - +0.0 808,626 - +0.3 808,626 - +0.3 808,626 - +0.1 808,626 - +0.8 808,626 - +1.5 808,626 - -1.6 808,626 - +0.5 808,626 - -0.4 808,626 - +0.4 808,626 - +0.1 808,626 - -0.1 808,626 - -0.8 808,626 - -0.1 808,626 - -0.4 808,626 - +0.1 808,626 - -1.6 808,626 - -0.4 808,626 - -3.6 808,626 - -0.1 808,626 - +1.5 808,626 - +0.7 808,626 - +0.1 808,631 - -2.4 808,683 + +0.3 808,685 + -0.7 808,690 + +1.4 808,694 + +0.9 808,695 + -0.3 808,704 + +0.3 808,704 + +0.3 808,704 + +1.9 808,712 + -0.6 808,717 + -0.3 808,717 + -1.0 808,764 + MPMX20_00774 0.19 -0.6 808,764 + MPMX20_00774 0.19 +0.9 808,831 + MPMX20_00774 0.32 +0.3 808,839 + MPMX20_00774 0.34 -1.4 808,844 + MPMX20_00774 0.35 +0.1 808,844 + MPMX20_00774 0.35 -1.1 808,844 + MPMX20_00774 0.35 -1.3 808,844 + MPMX20_00774 0.35 -0.7 808,844 + MPMX20_00774 0.35 -0.6 808,969 + MPMX20_00774 0.60 -0.4 808,989 + MPMX20_00774 0.64 -0.6 809,013 + MPMX20_00774 0.69 -0.0 809,045 + MPMX20_00774 0.76 +0.1 809,053 + MPMX20_00774 0.77 +0.2 809,053 + MPMX20_00774 0.77 +0.7 809,061 + MPMX20_00774 0.79 +0.3 809,080 + MPMX20_00774 0.83 -0.6 809,080 + MPMX20_00774 0.83 +0.6 809,080 + MPMX20_00774 0.83 -0.3 809,080 + MPMX20_00774 0.83 -0.4 809,080 + MPMX20_00774 0.83 +0.3 809,080 + MPMX20_00774 0.83 -1.1 809,080 + MPMX20_00774 0.83 +1.2 809,080 + MPMX20_00774 0.83 +2.9 809,090 + MPMX20_00774 0.85 +1.5 809,090 + MPMX20_00774 0.85 -0.7 809,107 + MPMX20_00774 0.88 +0.3 809,107 + MPMX20_00774 0.88 +0.4 809,160 + +1.7 809,320 + MPMX20_00775 0.16 -0.3 809,364 + MPMX20_00775 0.21 +0.3 809,481 + MPMX20_00775 0.33 -2.5 809,569 + MPMX20_00775 0.43 +0.3 809,620 + MPMX20_00775 0.48 +0.3 809,711 + MPMX20_00775 0.58 -0.9 809,789 + MPMX20_00775 0.66 -2.1 810,072 + -0.4 810,486 + +8.3
Or see this region's nucleotide sequence