Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_041 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00637 and MPMX20_00638 are separated by 9 nucleotides MPMX20_00638 and MPMX20_00639 are separated by 78 nucleotides MPMX20_00639 and MPMX20_00640 are separated by 9 nucleotides MPMX20_00640 and MPMX20_00641 are separated by 2 nucleotides
MPMX20_00637: MPMX20_00637 - 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, at 663,000 to 663,797
_00637
MPMX20_00638: MPMX20_00638 - 2-oxo-hept-4-ene-1,7-dioate hydratase, at 663,807 to 664,610
_00638
MPMX20_00639: MPMX20_00639 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 664,689 to 665,069
_00639
MPMX20_00640: MPMX20_00640 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 665,079 to 665,936
_00640
MPMX20_00641: MPMX20_00641 - NAD/NADP-dependent betaine aldehyde dehydrogenase, at 665,939 to 667,405
_00641
Position (kb) 664
665
666 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 663.729 kb on + strand at 663.732 kb on - strand at 663.732 kb on - strand at 663.734 kb on + strand at 663.734 kb on + strand at 663.734 kb on + strand at 663.734 kb on + strand at 663.737 kb on - strand at 663.742 kb on - strand at 663.812 kb on - strand at 663.833 kb on - strand at 663.833 kb on - strand at 663.844 kb on - strand at 663.859 kb on + strand at 663.859 kb on + strand at 663.867 kb on - strand at 663.867 kb on - strand at 663.878 kb on + strand at 663.927 kb on - strand, within MPMX20_00638 at 663.932 kb on - strand, within MPMX20_00638 at 664.030 kb on + strand, within MPMX20_00638 at 664.040 kb on + strand, within MPMX20_00638 at 664.053 kb on + strand, within MPMX20_00638 at 664.066 kb on - strand, within MPMX20_00638 at 664.066 kb on - strand, within MPMX20_00638 at 664.095 kb on + strand, within MPMX20_00638 at 664.095 kb on + strand, within MPMX20_00638 at 664.103 kb on - strand, within MPMX20_00638 at 664.108 kb on - strand, within MPMX20_00638 at 664.137 kb on - strand, within MPMX20_00638 at 664.147 kb on - strand, within MPMX20_00638 at 664.281 kb on + strand, within MPMX20_00638 at 664.281 kb on + strand, within MPMX20_00638 at 664.359 kb on + strand, within MPMX20_00638 at 664.364 kb on + strand, within MPMX20_00638 at 664.364 kb on + strand, within MPMX20_00638 at 664.369 kb on + strand, within MPMX20_00638 at 664.416 kb on + strand, within MPMX20_00638 at 664.416 kb on + strand, within MPMX20_00638 at 664.472 kb on + strand, within MPMX20_00638 at 664.474 kb on - strand, within MPMX20_00638 at 664.482 kb on - strand, within MPMX20_00638 at 664.484 kb on + strand, within MPMX20_00638 at 664.489 kb on + strand, within MPMX20_00638 at 664.492 kb on - strand, within MPMX20_00638 at 664.492 kb on - strand, within MPMX20_00638 at 664.511 kb on + strand, within MPMX20_00638 at 664.551 kb on + strand at 664.561 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.586 kb on + strand at 664.593 kb on + strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.594 kb on - strand at 664.878 kb on + strand, within MPMX20_00639 at 664.894 kb on + strand, within MPMX20_00639 at 664.924 kb on - strand, within MPMX20_00639 at 664.942 kb on + strand, within MPMX20_00639 at 664.944 kb on + strand, within MPMX20_00639 at 665.086 kb on + strand at 665.112 kb on + strand at 665.112 kb on + strand at 665.115 kb on - strand at 665.120 kb on - strand at 665.122 kb on + strand at 665.178 kb on - strand, within MPMX20_00640 at 665.233 kb on - strand, within MPMX20_00640 at 665.260 kb on + strand, within MPMX20_00640 at 665.268 kb on - strand, within MPMX20_00640 at 665.312 kb on - strand, within MPMX20_00640 at 665.338 kb on - strand, within MPMX20_00640 at 665.343 kb on - strand, within MPMX20_00640 at 665.453 kb on + strand, within MPMX20_00640 at 665.453 kb on + strand, within MPMX20_00640 at 665.467 kb on - strand, within MPMX20_00640 at 665.478 kb on + strand, within MPMX20_00640 at 665.478 kb on + strand, within MPMX20_00640 at 665.478 kb on + strand, within MPMX20_00640 at 665.486 kb on - strand, within MPMX20_00640 at 665.486 kb on - strand, within MPMX20_00640 at 665.486 kb on - strand, within MPMX20_00640 at 665.488 kb on + strand, within MPMX20_00640 at 665.488 kb on + strand, within MPMX20_00640 at 665.496 kb on - strand, within MPMX20_00640 at 665.496 kb on - strand, within MPMX20_00640 at 665.496 kb on - strand, within MPMX20_00640 at 665.512 kb on + strand, within MPMX20_00640 at 665.522 kb on + strand, within MPMX20_00640 at 665.522 kb on + strand, within MPMX20_00640 at 665.557 kb on - strand, within MPMX20_00640 at 665.568 kb on - strand, within MPMX20_00640 at 665.637 kb on - strand, within MPMX20_00640 at 665.715 kb on - strand, within MPMX20_00640 at 665.729 kb on - strand, within MPMX20_00640 at 665.752 kb on - strand, within MPMX20_00640 at 665.752 kb on - strand, within MPMX20_00640 at 665.752 kb on - strand, within MPMX20_00640 at 665.754 kb on + strand, within MPMX20_00640 at 665.788 kb on - strand, within MPMX20_00640 at 665.801 kb on + strand, within MPMX20_00640 at 665.809 kb on - strand, within MPMX20_00640 at 665.876 kb on + strand at 665.876 kb on + strand at 665.891 kb on - strand at 665.928 kb on - strand at 666.057 kb on - strand at 666.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_041 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 663,729 + -0.6 663,732 - +0.1 663,732 - +1.1 663,734 + +0.4 663,734 + -1.8 663,734 + -1.2 663,734 + +0.2 663,737 - -0.3 663,742 - +0.4 663,812 - -1.3 663,833 - +0.1 663,833 - -0.6 663,844 - +1.1 663,859 + -0.6 663,859 + +0.1 663,867 - -2.4 663,867 - -1.1 663,878 + -0.1 663,927 - MPMX20_00638 0.15 -0.3 663,932 - MPMX20_00638 0.16 -1.9 664,030 + MPMX20_00638 0.28 -2.2 664,040 + MPMX20_00638 0.29 +0.3 664,053 + MPMX20_00638 0.31 +1.0 664,066 - MPMX20_00638 0.32 -1.3 664,066 - MPMX20_00638 0.32 +0.7 664,095 + MPMX20_00638 0.36 -1.2 664,095 + MPMX20_00638 0.36 +0.8 664,103 - MPMX20_00638 0.37 -0.9 664,108 - MPMX20_00638 0.37 -0.8 664,137 - MPMX20_00638 0.41 -1.6 664,147 - MPMX20_00638 0.42 -1.3 664,281 + MPMX20_00638 0.59 +2.5 664,281 + MPMX20_00638 0.59 -0.3 664,359 + MPMX20_00638 0.69 -1.0 664,364 + MPMX20_00638 0.69 -1.8 664,364 + MPMX20_00638 0.69 -1.6 664,369 + MPMX20_00638 0.70 -0.3 664,416 + MPMX20_00638 0.76 -0.3 664,416 + MPMX20_00638 0.76 -0.8 664,472 + MPMX20_00638 0.83 +0.4 664,474 - MPMX20_00638 0.83 -0.9 664,482 - MPMX20_00638 0.84 +1.1 664,484 + MPMX20_00638 0.84 +0.7 664,489 + MPMX20_00638 0.85 -0.9 664,492 - MPMX20_00638 0.85 +1.7 664,492 - MPMX20_00638 0.85 -0.2 664,511 + MPMX20_00638 0.88 -0.3 664,551 + -0.7 664,561 + +0.2 664,586 + +1.0 664,586 + -0.3 664,586 + -0.9 664,586 + -0.2 664,586 + -0.3 664,586 + -0.8 664,586 + +0.1 664,586 + -0.1 664,586 + +0.6 664,586 + +0.9 664,593 + -0.6 664,594 - +0.3 664,594 - -1.4 664,594 - -0.7 664,594 - +1.3 664,594 - -1.2 664,594 - -1.1 664,594 - -0.3 664,594 - -1.9 664,594 - -0.1 664,594 - -0.1 664,878 + MPMX20_00639 0.50 +4.2 664,894 + MPMX20_00639 0.54 +1.9 664,924 - MPMX20_00639 0.62 +0.1 664,942 + MPMX20_00639 0.66 +0.2 664,944 + MPMX20_00639 0.67 +1.7 665,086 + -0.2 665,112 + -1.8 665,112 + -0.1 665,115 - +0.9 665,120 - +0.1 665,122 + +0.5 665,178 - MPMX20_00640 0.12 +0.8 665,233 - MPMX20_00640 0.18 -0.6 665,260 + MPMX20_00640 0.21 -0.3 665,268 - MPMX20_00640 0.22 +6.7 665,312 - MPMX20_00640 0.27 +0.1 665,338 - MPMX20_00640 0.30 -1.2 665,343 - MPMX20_00640 0.31 +0.1 665,453 + MPMX20_00640 0.44 -0.3 665,453 + MPMX20_00640 0.44 +0.7 665,467 - MPMX20_00640 0.45 -0.9 665,478 + MPMX20_00640 0.47 -0.3 665,478 + MPMX20_00640 0.47 -0.5 665,478 + MPMX20_00640 0.47 +0.6 665,486 - MPMX20_00640 0.47 +0.5 665,486 - MPMX20_00640 0.47 +0.1 665,486 - MPMX20_00640 0.47 -0.8 665,488 + MPMX20_00640 0.48 +0.1 665,488 + MPMX20_00640 0.48 -0.0 665,496 - MPMX20_00640 0.49 -0.2 665,496 - MPMX20_00640 0.49 +0.4 665,496 - MPMX20_00640 0.49 -0.3 665,512 + MPMX20_00640 0.50 +0.2 665,522 + MPMX20_00640 0.52 -0.3 665,522 + MPMX20_00640 0.52 +1.0 665,557 - MPMX20_00640 0.56 -0.6 665,568 - MPMX20_00640 0.57 +0.9 665,637 - MPMX20_00640 0.65 -0.1 665,715 - MPMX20_00640 0.74 +0.3 665,729 - MPMX20_00640 0.76 +0.3 665,752 - MPMX20_00640 0.78 -0.6 665,752 - MPMX20_00640 0.78 +0.2 665,752 - MPMX20_00640 0.78 -1.6 665,754 + MPMX20_00640 0.79 +1.0 665,788 - MPMX20_00640 0.83 -0.2 665,801 + MPMX20_00640 0.84 +0.9 665,809 - MPMX20_00640 0.85 -2.0 665,876 + -1.5 665,876 + -0.5 665,891 - +0.7 665,928 - +0.0 666,057 - +0.1 666,057 - +1.7
Or see this region's nucleotide sequence