Experiment: 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_065 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00430 and MPMX20_00431 overlap by 4 nucleotides MPMX20_00431 and MPMX20_00432 are separated by 98 nucleotides MPMX20_00432 and MPMX20_00433 are separated by 132 nucleotides
MPMX20_00430: MPMX20_00430 - Guanidinium exporter, at 444,423 to 444,740
_00430
MPMX20_00431: MPMX20_00431 - Outer membrane lipoprotein Blc, at 444,737 to 445,267
_00431
MPMX20_00432: MPMX20_00432 - Beta-lactamase, at 445,366 to 446,511
_00432
MPMX20_00433: MPMX20_00433 - HTH-type transcriptional activator AmpR, at 446,644 to 447,519
_00433
Position (kb) 445
446
447 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 444.370 kb on - strand at 444.377 kb on - strand at 444.390 kb on + strand at 444.430 kb on + strand at 444.463 kb on - strand, within MPMX20_00430 at 444.477 kb on + strand, within MPMX20_00430 at 444.477 kb on + strand, within MPMX20_00430 at 444.478 kb on + strand, within MPMX20_00430 at 444.485 kb on - strand, within MPMX20_00430 at 444.485 kb on - strand, within MPMX20_00430 at 444.485 kb on - strand, within MPMX20_00430 at 444.496 kb on + strand, within MPMX20_00430 at 444.510 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.520 kb on + strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.528 kb on - strand, within MPMX20_00430 at 444.592 kb on - strand, within MPMX20_00430 at 444.592 kb on - strand, within MPMX20_00430 at 444.592 kb on - strand, within MPMX20_00430 at 444.594 kb on + strand, within MPMX20_00430 at 444.698 kb on + strand, within MPMX20_00430 at 444.698 kb on + strand, within MPMX20_00430 at 444.702 kb on - strand, within MPMX20_00430 at 444.725 kb on + strand at 444.748 kb on + strand at 444.748 kb on + strand at 444.748 kb on + strand at 444.756 kb on - strand at 444.756 kb on - strand at 444.756 kb on - strand at 444.756 kb on - strand at 444.756 kb on - strand at 444.758 kb on + strand at 444.772 kb on + strand at 444.772 kb on + strand at 444.780 kb on - strand at 444.780 kb on - strand at 444.829 kb on + strand, within MPMX20_00431 at 444.837 kb on - strand, within MPMX20_00431 at 444.837 kb on - strand, within MPMX20_00431 at 444.860 kb on + strand, within MPMX20_00431 at 444.881 kb on - strand, within MPMX20_00431 at 444.883 kb on + strand, within MPMX20_00431 at 444.891 kb on - strand, within MPMX20_00431 at 444.903 kb on - strand, within MPMX20_00431 at 444.959 kb on - strand, within MPMX20_00431 at 444.984 kb on + strand, within MPMX20_00431 at 445.048 kb on + strand, within MPMX20_00431 at 445.048 kb on + strand, within MPMX20_00431 at 445.048 kb on + strand, within MPMX20_00431 at 445.091 kb on - strand, within MPMX20_00431 at 445.091 kb on - strand, within MPMX20_00431 at 445.091 kb on - strand, within MPMX20_00431 at 445.101 kb on - strand, within MPMX20_00431 at 445.103 kb on - strand, within MPMX20_00431 at 445.143 kb on - strand, within MPMX20_00431 at 445.188 kb on - strand, within MPMX20_00431 at 445.303 kb on - strand at 445.354 kb on + strand at 445.418 kb on + strand at 445.435 kb on + strand at 445.445 kb on - strand at 445.515 kb on + strand, within MPMX20_00432 at 445.515 kb on + strand, within MPMX20_00432 at 445.544 kb on + strand, within MPMX20_00432 at 445.561 kb on + strand, within MPMX20_00432 at 445.606 kb on + strand, within MPMX20_00432 at 445.642 kb on + strand, within MPMX20_00432 at 445.642 kb on + strand, within MPMX20_00432 at 445.650 kb on - strand, within MPMX20_00432 at 445.650 kb on - strand, within MPMX20_00432 at 445.650 kb on - strand, within MPMX20_00432 at 445.665 kb on - strand, within MPMX20_00432 at 445.702 kb on - strand, within MPMX20_00432 at 445.702 kb on - strand, within MPMX20_00432 at 445.760 kb on + strand, within MPMX20_00432 at 445.768 kb on - strand, within MPMX20_00432 at 445.841 kb on + strand, within MPMX20_00432 at 445.849 kb on - strand, within MPMX20_00432 at 445.849 kb on - strand, within MPMX20_00432 at 445.855 kb on - strand, within MPMX20_00432 at 445.855 kb on - strand, within MPMX20_00432 at 445.861 kb on - strand, within MPMX20_00432 at 445.861 kb on - strand, within MPMX20_00432 at 445.865 kb on + strand, within MPMX20_00432 at 445.880 kb on + strand, within MPMX20_00432 at 445.905 kb on - strand, within MPMX20_00432 at 445.910 kb on - strand, within MPMX20_00432 at 445.936 kb on - strand, within MPMX20_00432 at 445.973 kb on + strand, within MPMX20_00432 at 445.973 kb on + strand, within MPMX20_00432 at 445.973 kb on + strand, within MPMX20_00432 at 445.981 kb on - strand, within MPMX20_00432 at 445.981 kb on - strand, within MPMX20_00432 at 446.112 kb on + strand, within MPMX20_00432 at 446.135 kb on - strand, within MPMX20_00432 at 446.166 kb on + strand, within MPMX20_00432 at 446.166 kb on + strand, within MPMX20_00432 at 446.169 kb on - strand, within MPMX20_00432 at 446.174 kb on - strand, within MPMX20_00432 at 446.174 kb on - strand, within MPMX20_00432 at 446.184 kb on - strand, within MPMX20_00432 at 446.297 kb on - strand, within MPMX20_00432 at 446.302 kb on + strand, within MPMX20_00432 at 446.302 kb on - strand, within MPMX20_00432 at 446.343 kb on + strand, within MPMX20_00432 at 446.351 kb on - strand, within MPMX20_00432 at 446.420 kb on + strand at 446.428 kb on - strand at 446.452 kb on + strand at 446.452 kb on + strand at 446.452 kb on + strand at 446.460 kb on - strand at 446.460 kb on - strand at 446.471 kb on + strand at 446.533 kb on + strand at 446.541 kb on - strand at 446.541 kb on - strand at 446.541 kb on - strand at 446.541 kb on - strand at 446.559 kb on + strand at 446.771 kb on - strand, within MPMX20_00433 at 446.779 kb on - strand, within MPMX20_00433 at 446.782 kb on + strand, within MPMX20_00433 at 446.790 kb on - strand, within MPMX20_00433 at 446.790 kb on - strand, within MPMX20_00433 at 446.790 kb on - strand, within MPMX20_00433 at 446.819 kb on + strand, within MPMX20_00433 at 446.819 kb on + strand, within MPMX20_00433 at 446.819 kb on + strand, within MPMX20_00433 at 446.827 kb on - strand, within MPMX20_00433 at 446.893 kb on + strand, within MPMX20_00433 at 447.008 kb on + strand, within MPMX20_00433 at 447.009 kb on + strand, within MPMX20_00433 at 447.074 kb on - strand, within MPMX20_00433 at 447.074 kb on - strand, within MPMX20_00433 at 447.084 kb on + strand, within MPMX20_00433 at 447.086 kb on + strand, within MPMX20_00433 at 447.094 kb on - strand, within MPMX20_00433 at 447.117 kb on - strand, within MPMX20_00433 at 447.128 kb on - strand, within MPMX20_00433 at 447.147 kb on + strand, within MPMX20_00433 at 447.197 kb on + strand, within MPMX20_00433 at 447.205 kb on + strand, within MPMX20_00433 at 447.207 kb on + strand, within MPMX20_00433 at 447.246 kb on - strand, within MPMX20_00433 at 447.272 kb on + strand, within MPMX20_00433 at 447.292 kb on + strand, within MPMX20_00433 at 447.378 kb on + strand, within MPMX20_00433 at 447.424 kb on - strand, within MPMX20_00433 at 447.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_065 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 444,370 - +0.7 444,377 - +0.3 444,390 + -1.1 444,430 + +1.7 444,463 - MPMX20_00430 0.13 +0.3 444,477 + MPMX20_00430 0.17 +0.9 444,477 + MPMX20_00430 0.17 -0.5 444,478 + MPMX20_00430 0.17 -1.2 444,485 - MPMX20_00430 0.19 -1.3 444,485 - MPMX20_00430 0.19 -1.5 444,485 - MPMX20_00430 0.19 -1.1 444,496 + MPMX20_00430 0.23 -1.5 444,510 + MPMX20_00430 0.27 +1.0 444,520 + MPMX20_00430 0.31 -1.0 444,520 + MPMX20_00430 0.31 +1.7 444,520 + MPMX20_00430 0.31 +0.1 444,520 + MPMX20_00430 0.31 -1.1 444,520 + MPMX20_00430 0.31 +0.6 444,520 + MPMX20_00430 0.31 -1.3 444,520 + MPMX20_00430 0.31 -0.5 444,520 + MPMX20_00430 0.31 -1.3 444,528 - MPMX20_00430 0.33 -0.5 444,528 - MPMX20_00430 0.33 +1.7 444,528 - MPMX20_00430 0.33 +0.5 444,528 - MPMX20_00430 0.33 +0.7 444,528 - MPMX20_00430 0.33 -1.6 444,528 - MPMX20_00430 0.33 -1.8 444,528 - MPMX20_00430 0.33 +0.3 444,528 - MPMX20_00430 0.33 +0.7 444,592 - MPMX20_00430 0.53 +0.3 444,592 - MPMX20_00430 0.53 +0.1 444,592 - MPMX20_00430 0.53 -0.5 444,594 + MPMX20_00430 0.54 +2.7 444,698 + MPMX20_00430 0.86 -1.4 444,698 + MPMX20_00430 0.86 +0.3 444,702 - MPMX20_00430 0.88 -0.2 444,725 + -0.8 444,748 + -1.7 444,748 + -2.2 444,748 + +1.9 444,756 - +0.5 444,756 - +0.9 444,756 - -0.7 444,756 - +0.9 444,756 - +0.3 444,758 + +1.1 444,772 + +0.1 444,772 + -1.3 444,780 - -0.8 444,780 - -0.8 444,829 + MPMX20_00431 0.17 -1.2 444,837 - MPMX20_00431 0.19 +1.1 444,837 - MPMX20_00431 0.19 +1.7 444,860 + MPMX20_00431 0.23 -0.5 444,881 - MPMX20_00431 0.27 -1.0 444,883 + MPMX20_00431 0.27 -0.3 444,891 - MPMX20_00431 0.29 -1.1 444,903 - MPMX20_00431 0.31 -0.7 444,959 - MPMX20_00431 0.42 -0.7 444,984 + MPMX20_00431 0.47 +1.7 445,048 + MPMX20_00431 0.59 +1.1 445,048 + MPMX20_00431 0.59 -0.3 445,048 + MPMX20_00431 0.59 +0.9 445,091 - MPMX20_00431 0.67 -0.1 445,091 - MPMX20_00431 0.67 -0.9 445,091 - MPMX20_00431 0.67 -1.2 445,101 - MPMX20_00431 0.69 +0.3 445,103 - MPMX20_00431 0.69 +0.9 445,143 - MPMX20_00431 0.76 -1.3 445,188 - MPMX20_00431 0.85 -1.1 445,303 - -2.3 445,354 + -1.4 445,418 + +6.0 445,435 + +0.1 445,445 - +1.1 445,515 + MPMX20_00432 0.13 -1.3 445,515 + MPMX20_00432 0.13 +1.1 445,544 + MPMX20_00432 0.16 -0.8 445,561 + MPMX20_00432 0.17 +0.9 445,606 + MPMX20_00432 0.21 +0.1 445,642 + MPMX20_00432 0.24 -1.2 445,642 + MPMX20_00432 0.24 -0.2 445,650 - MPMX20_00432 0.25 -1.7 445,650 - MPMX20_00432 0.25 -1.3 445,650 - MPMX20_00432 0.25 -1.5 445,665 - MPMX20_00432 0.26 +0.1 445,702 - MPMX20_00432 0.29 -0.9 445,702 - MPMX20_00432 0.29 -2.0 445,760 + MPMX20_00432 0.34 +0.1 445,768 - MPMX20_00432 0.35 +1.2 445,841 + MPMX20_00432 0.41 +0.7 445,849 - MPMX20_00432 0.42 -0.8 445,849 - MPMX20_00432 0.42 -1.7 445,855 - MPMX20_00432 0.43 -1.0 445,855 - MPMX20_00432 0.43 -2.1 445,861 - MPMX20_00432 0.43 +0.3 445,861 - MPMX20_00432 0.43 -1.1 445,865 + MPMX20_00432 0.44 -1.5 445,880 + MPMX20_00432 0.45 -1.4 445,905 - MPMX20_00432 0.47 +1.1 445,910 - MPMX20_00432 0.47 +2.7 445,936 - MPMX20_00432 0.50 +1.1 445,973 + MPMX20_00432 0.53 -2.0 445,973 + MPMX20_00432 0.53 +0.1 445,973 + MPMX20_00432 0.53 +0.6 445,981 - MPMX20_00432 0.54 -0.9 445,981 - MPMX20_00432 0.54 +1.7 446,112 + MPMX20_00432 0.65 -1.3 446,135 - MPMX20_00432 0.67 +0.9 446,166 + MPMX20_00432 0.70 -1.0 446,166 + MPMX20_00432 0.70 -0.7 446,169 - MPMX20_00432 0.70 +0.7 446,174 - MPMX20_00432 0.71 -1.3 446,174 - MPMX20_00432 0.71 -1.1 446,184 - MPMX20_00432 0.71 +0.1 446,297 - MPMX20_00432 0.81 +0.3 446,302 + MPMX20_00432 0.82 +0.2 446,302 - MPMX20_00432 0.82 -1.1 446,343 + MPMX20_00432 0.85 +0.1 446,351 - MPMX20_00432 0.86 -2.1 446,420 + -0.9 446,428 - +0.1 446,452 + +1.7 446,452 + -0.7 446,452 + +1.7 446,460 - +0.1 446,460 - -0.3 446,471 + +0.7 446,533 + -1.5 446,541 - +0.7 446,541 - +2.7 446,541 - -1.0 446,541 - +0.9 446,559 + +0.3 446,771 - MPMX20_00433 0.14 +1.7 446,779 - MPMX20_00433 0.15 -0.6 446,782 + MPMX20_00433 0.16 -2.0 446,790 - MPMX20_00433 0.17 +0.2 446,790 - MPMX20_00433 0.17 -1.0 446,790 - MPMX20_00433 0.17 -1.8 446,819 + MPMX20_00433 0.20 -0.0 446,819 + MPMX20_00433 0.20 +0.3 446,819 + MPMX20_00433 0.20 -2.7 446,827 - MPMX20_00433 0.21 -0.9 446,893 + MPMX20_00433 0.28 +1.7 447,008 + MPMX20_00433 0.42 -0.3 447,009 + MPMX20_00433 0.42 -1.1 447,074 - MPMX20_00433 0.49 +0.7 447,074 - MPMX20_00433 0.49 -2.5 447,084 + MPMX20_00433 0.50 +1.1 447,086 + MPMX20_00433 0.50 -2.4 447,094 - MPMX20_00433 0.51 -0.8 447,117 - MPMX20_00433 0.54 -0.6 447,128 - MPMX20_00433 0.55 -0.1 447,147 + MPMX20_00433 0.57 +0.1 447,197 + MPMX20_00433 0.63 -0.5 447,205 + MPMX20_00433 0.64 +0.3 447,207 + MPMX20_00433 0.64 -0.7 447,246 - MPMX20_00433 0.69 +0.3 447,272 + MPMX20_00433 0.72 -0.1 447,292 + MPMX20_00433 0.74 -0.1 447,378 + MPMX20_00433 0.84 -0.5 447,424 - MPMX20_00433 0.89 +0.7 447,475 - +0.3
Or see this region's nucleotide sequence