Experiment: 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_065 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00319 and MPMX20_00320 overlap by 1 nucleotides MPMX20_00320 and MPMX20_00321 overlap by 4 nucleotides MPMX20_00321 and MPMX20_00322 are separated by 0 nucleotides MPMX20_00322 and MPMX20_00323 are separated by 20 nucleotides
MPMX20_00319: MPMX20_00319 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI, at 338,723 to 339,787
_00319
MPMX20_00320: MPMX20_00320 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH, at 339,787 to 340,371
_00320
MPMX20_00321: MPMX20_00321 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG, at 340,368 to 340,820
_00321
MPMX20_00322: MPMX20_00322 - putative transcriptional regulator PhnF, at 340,821 to 341,546
_00322
MPMX20_00323: MPMX20_00323 - Phosphate-import permease protein PhnE, at 341,567 to 342,346
_00323
Position (kb) 340
341 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 339.370 kb on - strand, within MPMX20_00319 at 339.399 kb on - strand, within MPMX20_00319 at 339.427 kb on - strand, within MPMX20_00319 at 339.429 kb on + strand, within MPMX20_00319 at 339.491 kb on + strand, within MPMX20_00319 at 339.590 kb on - strand, within MPMX20_00319 at 339.630 kb on - strand, within MPMX20_00319 at 339.639 kb on + strand, within MPMX20_00319 at 339.647 kb on - strand, within MPMX20_00319 at 339.682 kb on + strand at 339.780 kb on + strand at 339.798 kb on + strand at 339.850 kb on - strand, within MPMX20_00320 at 339.850 kb on - strand, within MPMX20_00320 at 339.850 kb on - strand, within MPMX20_00320 at 339.860 kb on - strand, within MPMX20_00320 at 339.860 kb on - strand, within MPMX20_00320 at 339.860 kb on - strand, within MPMX20_00320 at 339.860 kb on - strand, within MPMX20_00320 at 339.860 kb on - strand, within MPMX20_00320 at 339.868 kb on + strand, within MPMX20_00320 at 339.881 kb on + strand, within MPMX20_00320 at 339.891 kb on + strand, within MPMX20_00320 at 339.899 kb on - strand, within MPMX20_00320 at 339.902 kb on + strand, within MPMX20_00320 at 339.915 kb on + strand, within MPMX20_00320 at 339.934 kb on - strand, within MPMX20_00320 at 339.958 kb on - strand, within MPMX20_00320 at 339.979 kb on + strand, within MPMX20_00320 at 339.992 kb on - strand, within MPMX20_00320 at 339.992 kb on - strand, within MPMX20_00320 at 340.018 kb on - strand, within MPMX20_00320 at 340.049 kb on + strand, within MPMX20_00320 at 340.049 kb on + strand, within MPMX20_00320 at 340.057 kb on - strand, within MPMX20_00320 at 340.100 kb on + strand, within MPMX20_00320 at 340.124 kb on + strand, within MPMX20_00320 at 340.138 kb on + strand, within MPMX20_00320 at 340.247 kb on + strand, within MPMX20_00320 at 340.260 kb on - strand, within MPMX20_00320 at 340.301 kb on + strand, within MPMX20_00320 at 340.301 kb on + strand, within MPMX20_00320 at 340.347 kb on + strand at 340.347 kb on + strand at 340.389 kb on - strand at 340.391 kb on + strand at 340.425 kb on + strand, within MPMX20_00321 at 340.440 kb on + strand, within MPMX20_00321 at 340.445 kb on + strand, within MPMX20_00321 at 340.445 kb on + strand, within MPMX20_00321 at 340.448 kb on - strand, within MPMX20_00321 at 340.448 kb on - strand, within MPMX20_00321 at 340.459 kb on + strand, within MPMX20_00321 at 340.459 kb on + strand, within MPMX20_00321 at 340.525 kb on + strand, within MPMX20_00321 at 340.539 kb on + strand, within MPMX20_00321 at 340.547 kb on - strand, within MPMX20_00321 at 340.584 kb on - strand, within MPMX20_00321 at 340.596 kb on + strand, within MPMX20_00321 at 340.596 kb on + strand, within MPMX20_00321 at 340.596 kb on + strand, within MPMX20_00321 at 340.604 kb on - strand, within MPMX20_00321 at 340.604 kb on - strand, within MPMX20_00321 at 340.625 kb on - strand, within MPMX20_00321 at 340.644 kb on + strand, within MPMX20_00321 at 340.649 kb on + strand, within MPMX20_00321 at 340.657 kb on - strand, within MPMX20_00321 at 340.657 kb on - strand, within MPMX20_00321 at 340.659 kb on + strand, within MPMX20_00321 at 340.667 kb on - strand, within MPMX20_00321 at 340.687 kb on - strand, within MPMX20_00321 at 340.702 kb on + strand, within MPMX20_00321 at 340.789 kb on - strand at 340.789 kb on - strand at 340.856 kb on + strand at 340.857 kb on + strand at 340.864 kb on - strand at 340.880 kb on - strand at 340.904 kb on + strand, within MPMX20_00322 at 340.912 kb on - strand, within MPMX20_00322 at 340.912 kb on - strand, within MPMX20_00322 at 340.912 kb on - strand, within MPMX20_00322 at 340.912 kb on - strand, within MPMX20_00322 at 340.912 kb on - strand, within MPMX20_00322 at 340.912 kb on - strand, within MPMX20_00322 at 340.918 kb on + strand, within MPMX20_00322 at 340.921 kb on - strand, within MPMX20_00322 at 340.931 kb on - strand, within MPMX20_00322 at 340.947 kb on + strand, within MPMX20_00322 at 340.962 kb on + strand, within MPMX20_00322 at 340.962 kb on + strand, within MPMX20_00322 at 340.971 kb on - strand, within MPMX20_00322 at 340.984 kb on + strand, within MPMX20_00322 at 341.013 kb on - strand, within MPMX20_00322 at 341.095 kb on + strand, within MPMX20_00322 at 341.095 kb on + strand, within MPMX20_00322 at 341.095 kb on + strand, within MPMX20_00322 at 341.098 kb on + strand, within MPMX20_00322 at 341.100 kb on + strand, within MPMX20_00322 at 341.100 kb on + strand, within MPMX20_00322 at 341.100 kb on + strand, within MPMX20_00322 at 341.110 kb on + strand, within MPMX20_00322 at 341.118 kb on - strand, within MPMX20_00322 at 341.118 kb on - strand, within MPMX20_00322 at 341.136 kb on - strand, within MPMX20_00322 at 341.146 kb on + strand, within MPMX20_00322 at 341.146 kb on + strand, within MPMX20_00322 at 341.153 kb on + strand, within MPMX20_00322 at 341.182 kb on - strand, within MPMX20_00322 at 341.202 kb on + strand, within MPMX20_00322 at 341.210 kb on - strand, within MPMX20_00322 at 341.275 kb on - strand, within MPMX20_00322 at 341.316 kb on + strand, within MPMX20_00322 at 341.324 kb on - strand, within MPMX20_00322 at 341.336 kb on + strand, within MPMX20_00322 at 341.338 kb on + strand, within MPMX20_00322 at 341.346 kb on + strand, within MPMX20_00322 at 341.351 kb on + strand, within MPMX20_00322 at 341.356 kb on + strand, within MPMX20_00322 at 341.359 kb on - strand, within MPMX20_00322 at 341.359 kb on - strand, within MPMX20_00322 at 341.359 kb on - strand, within MPMX20_00322 at 341.359 kb on - strand, within MPMX20_00322 at 341.383 kb on - strand, within MPMX20_00322 at 341.408 kb on + strand, within MPMX20_00322 at 341.432 kb on + strand, within MPMX20_00322 at 341.442 kb on + strand, within MPMX20_00322 at 341.487 kb on - strand at 341.498 kb on + strand at 341.498 kb on + strand at 341.568 kb on + strand at 341.635 kb on + strand at 341.635 kb on + strand at 341.643 kb on - strand at 341.692 kb on + strand, within MPMX20_00323 at 341.700 kb on - strand, within MPMX20_00323 at 341.771 kb on - strand, within MPMX20_00323 at 341.787 kb on + strand, within MPMX20_00323 at 341.795 kb on - strand, within MPMX20_00323 at 341.800 kb on - strand, within MPMX20_00323
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_065 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 339,370 - MPMX20_00319 0.61 -0.5 339,399 - MPMX20_00319 0.63 -1.5 339,427 - MPMX20_00319 0.66 -0.8 339,429 + MPMX20_00319 0.66 -1.5 339,491 + MPMX20_00319 0.72 -0.1 339,590 - MPMX20_00319 0.81 -2.8 339,630 - MPMX20_00319 0.85 -1.7 339,639 + MPMX20_00319 0.86 -1.9 339,647 - MPMX20_00319 0.87 -0.6 339,682 + -1.8 339,780 + -2.9 339,798 + +1.1 339,850 - MPMX20_00320 0.11 -0.7 339,850 - MPMX20_00320 0.11 +1.3 339,850 - MPMX20_00320 0.11 -1.5 339,860 - MPMX20_00320 0.12 -2.1 339,860 - MPMX20_00320 0.12 -0.2 339,860 - MPMX20_00320 0.12 -0.3 339,860 - MPMX20_00320 0.12 -0.7 339,860 - MPMX20_00320 0.12 +0.0 339,868 + MPMX20_00320 0.14 +2.7 339,881 + MPMX20_00320 0.16 +0.2 339,891 + MPMX20_00320 0.18 +0.5 339,899 - MPMX20_00320 0.19 -0.8 339,902 + MPMX20_00320 0.20 +1.4 339,915 + MPMX20_00320 0.22 +1.4 339,934 - MPMX20_00320 0.25 -0.3 339,958 - MPMX20_00320 0.29 +0.4 339,979 + MPMX20_00320 0.33 +0.7 339,992 - MPMX20_00320 0.35 +0.4 339,992 - MPMX20_00320 0.35 -1.8 340,018 - MPMX20_00320 0.39 +0.1 340,049 + MPMX20_00320 0.45 -0.1 340,049 + MPMX20_00320 0.45 -2.3 340,057 - MPMX20_00320 0.46 -2.4 340,100 + MPMX20_00320 0.54 +0.1 340,124 + MPMX20_00320 0.58 +1.2 340,138 + MPMX20_00320 0.60 -0.5 340,247 + MPMX20_00320 0.79 -1.2 340,260 - MPMX20_00320 0.81 -0.8 340,301 + MPMX20_00320 0.88 -1.8 340,301 + MPMX20_00320 0.88 +0.5 340,347 + -0.9 340,347 + -1.9 340,389 - -0.5 340,391 + +1.1 340,425 + MPMX20_00321 0.13 -0.8 340,440 + MPMX20_00321 0.16 -0.4 340,445 + MPMX20_00321 0.17 +0.9 340,445 + MPMX20_00321 0.17 -0.3 340,448 - MPMX20_00321 0.18 -1.9 340,448 - MPMX20_00321 0.18 +0.4 340,459 + MPMX20_00321 0.20 -0.6 340,459 + MPMX20_00321 0.20 +0.1 340,525 + MPMX20_00321 0.35 -0.8 340,539 + MPMX20_00321 0.38 +0.7 340,547 - MPMX20_00321 0.40 -2.3 340,584 - MPMX20_00321 0.48 +0.4 340,596 + MPMX20_00321 0.50 -0.3 340,596 + MPMX20_00321 0.50 +0.2 340,596 + MPMX20_00321 0.50 +0.4 340,604 - MPMX20_00321 0.52 +0.2 340,604 - MPMX20_00321 0.52 +1.1 340,625 - MPMX20_00321 0.57 -0.2 340,644 + MPMX20_00321 0.61 -0.6 340,649 + MPMX20_00321 0.62 -1.0 340,657 - MPMX20_00321 0.64 -1.8 340,657 - MPMX20_00321 0.64 -0.8 340,659 + MPMX20_00321 0.64 -0.3 340,667 - MPMX20_00321 0.66 +1.3 340,687 - MPMX20_00321 0.70 +1.5 340,702 + MPMX20_00321 0.74 -1.1 340,789 - -1.8 340,789 - -0.1 340,856 + +1.7 340,857 + +0.1 340,864 - -1.1 340,880 - -0.6 340,904 + MPMX20_00322 0.11 -0.2 340,912 - MPMX20_00322 0.13 -2.3 340,912 - MPMX20_00322 0.13 +2.6 340,912 - MPMX20_00322 0.13 +0.7 340,912 - MPMX20_00322 0.13 -0.9 340,912 - MPMX20_00322 0.13 -0.9 340,912 - MPMX20_00322 0.13 -1.5 340,918 + MPMX20_00322 0.13 -1.2 340,921 - MPMX20_00322 0.14 +0.9 340,931 - MPMX20_00322 0.15 -0.1 340,947 + MPMX20_00322 0.17 +0.0 340,962 + MPMX20_00322 0.19 -1.6 340,962 + MPMX20_00322 0.19 +1.4 340,971 - MPMX20_00322 0.21 +0.9 340,984 + MPMX20_00322 0.22 +0.1 341,013 - MPMX20_00322 0.26 +1.1 341,095 + MPMX20_00322 0.38 -0.8 341,095 + MPMX20_00322 0.38 -1.2 341,095 + MPMX20_00322 0.38 +1.7 341,098 + MPMX20_00322 0.38 -1.5 341,100 + MPMX20_00322 0.38 -0.8 341,100 + MPMX20_00322 0.38 +0.1 341,100 + MPMX20_00322 0.38 +1.1 341,110 + MPMX20_00322 0.40 +0.7 341,118 - MPMX20_00322 0.41 -0.4 341,118 - MPMX20_00322 0.41 +1.1 341,136 - MPMX20_00322 0.43 +0.1 341,146 + MPMX20_00322 0.45 +1.7 341,146 + MPMX20_00322 0.45 +0.7 341,153 + MPMX20_00322 0.46 -1.1 341,182 - MPMX20_00322 0.50 -0.8 341,202 + MPMX20_00322 0.52 +1.4 341,210 - MPMX20_00322 0.54 -2.3 341,275 - MPMX20_00322 0.63 -1.5 341,316 + MPMX20_00322 0.68 -1.2 341,324 - MPMX20_00322 0.69 -2.9 341,336 + MPMX20_00322 0.71 +1.1 341,338 + MPMX20_00322 0.71 +0.4 341,346 + MPMX20_00322 0.72 -0.2 341,351 + MPMX20_00322 0.73 -0.2 341,356 + MPMX20_00322 0.74 -1.1 341,359 - MPMX20_00322 0.74 +0.5 341,359 - MPMX20_00322 0.74 -0.4 341,359 - MPMX20_00322 0.74 -1.3 341,359 - MPMX20_00322 0.74 -0.0 341,383 - MPMX20_00322 0.77 +0.3 341,408 + MPMX20_00322 0.81 +0.7 341,432 + MPMX20_00322 0.84 -2.1 341,442 + MPMX20_00322 0.86 +0.5 341,487 - -1.1 341,498 + -1.2 341,498 + -0.3 341,568 + +1.5 341,635 + +0.6 341,635 + +0.7 341,643 - -2.0 341,692 + MPMX20_00323 0.16 -1.3 341,700 - MPMX20_00323 0.17 -1.7 341,771 - MPMX20_00323 0.26 +1.4 341,787 + MPMX20_00323 0.28 -0.4 341,795 - MPMX20_00323 0.29 -1.6 341,800 - MPMX20_00323 0.30 -0.9
Or see this region's nucleotide sequence