Experiment: M9 plate, Mixed culture; Bacillus subtilis TBS_102 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00302 and MPMX20_00303 are separated by 181 nucleotides MPMX20_00303 and MPMX20_00304 overlap by 89 nucleotides MPMX20_00304 and MPMX20_00305 overlap by 17 nucleotides
MPMX20_00302: MPMX20_00302 - Ribose import ATP-binding protein RbsA, at 322,290 to 323,810
_00302
MPMX20_00303: MPMX20_00303 - Sensor histidine kinase RcsC, at 323,992 to 326,313
_00303
MPMX20_00304: MPMX20_00304 - D-allose kinase, at 326,225 to 327,169
_00304
MPMX20_00305: MPMX20_00305 - D-allulose-6-phosphate 3-epimerase, at 327,153 to 327,848
_00305
Position (kb) 323
324
325
326
327 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 322.992 kb on + strand, within MPMX20_00302 at 322.993 kb on - strand, within MPMX20_00302 at 322.993 kb on - strand, within MPMX20_00302 at 322.995 kb on - strand, within MPMX20_00302 at 322.995 kb on - strand, within MPMX20_00302 at 323.027 kb on - strand, within MPMX20_00302 at 323.035 kb on - strand, within MPMX20_00302 at 323.056 kb on - strand, within MPMX20_00302 at 323.065 kb on + strand, within MPMX20_00302 at 323.065 kb on + strand, within MPMX20_00302 at 323.065 kb on + strand, within MPMX20_00302 at 323.111 kb on + strand, within MPMX20_00302 at 323.111 kb on + strand, within MPMX20_00302 at 323.111 kb on + strand, within MPMX20_00302 at 323.119 kb on - strand, within MPMX20_00302 at 323.119 kb on - strand, within MPMX20_00302 at 323.119 kb on - strand, within MPMX20_00302 at 323.119 kb on - strand, within MPMX20_00302 at 323.119 kb on - strand, within MPMX20_00302 at 323.159 kb on - strand, within MPMX20_00302 at 323.270 kb on + strand, within MPMX20_00302 at 323.284 kb on + strand, within MPMX20_00302 at 323.292 kb on - strand, within MPMX20_00302 at 323.292 kb on - strand, within MPMX20_00302 at 323.302 kb on - strand, within MPMX20_00302 at 323.340 kb on - strand, within MPMX20_00302 at 323.374 kb on + strand, within MPMX20_00302 at 323.382 kb on - strand, within MPMX20_00302 at 323.382 kb on - strand, within MPMX20_00302 at 323.384 kb on + strand, within MPMX20_00302 at 323.404 kb on + strand, within MPMX20_00302 at 323.412 kb on - strand, within MPMX20_00302 at 323.412 kb on - strand, within MPMX20_00302 at 323.468 kb on + strand, within MPMX20_00302 at 323.472 kb on - strand, within MPMX20_00302 at 323.476 kb on - strand, within MPMX20_00302 at 323.533 kb on - strand, within MPMX20_00302 at 323.649 kb on - strand, within MPMX20_00302 at 323.673 kb on + strand at 323.686 kb on - strand at 323.688 kb on + strand at 323.733 kb on + strand at 323.740 kb on + strand at 323.748 kb on - strand at 323.748 kb on - strand at 323.795 kb on - strand at 323.849 kb on - strand at 323.895 kb on + strand at 323.895 kb on + strand at 323.903 kb on - strand at 323.903 kb on - strand at 323.941 kb on + strand at 323.941 kb on + strand at 323.949 kb on - strand at 323.951 kb on + strand at 323.960 kb on - strand at 323.963 kb on + strand at 323.963 kb on + strand at 323.965 kb on - strand at 323.979 kb on - strand at 323.995 kb on + strand at 324.086 kb on - strand at 324.094 kb on - strand at 324.094 kb on - strand at 324.175 kb on - strand at 324.196 kb on - strand at 324.219 kb on + strand at 324.296 kb on + strand, within MPMX20_00303 at 324.304 kb on - strand, within MPMX20_00303 at 324.338 kb on - strand, within MPMX20_00303 at 324.338 kb on - strand, within MPMX20_00303 at 324.374 kb on + strand, within MPMX20_00303 at 324.374 kb on + strand, within MPMX20_00303 at 324.386 kb on + strand, within MPMX20_00303 at 324.421 kb on + strand, within MPMX20_00303 at 324.439 kb on - strand, within MPMX20_00303 at 324.439 kb on - strand, within MPMX20_00303 at 324.455 kb on + strand, within MPMX20_00303 at 324.548 kb on + strand, within MPMX20_00303 at 324.622 kb on - strand, within MPMX20_00303 at 324.622 kb on - strand, within MPMX20_00303 at 324.632 kb on + strand, within MPMX20_00303 at 324.632 kb on - strand, within MPMX20_00303 at 324.632 kb on - strand, within MPMX20_00303 at 324.653 kb on + strand, within MPMX20_00303 at 324.689 kb on + strand, within MPMX20_00303 at 324.697 kb on - strand, within MPMX20_00303 at 324.697 kb on - strand, within MPMX20_00303 at 324.697 kb on - strand, within MPMX20_00303 at 324.702 kb on - strand, within MPMX20_00303 at 324.718 kb on - strand, within MPMX20_00303 at 324.772 kb on + strand, within MPMX20_00303 at 324.805 kb on - strand, within MPMX20_00303 at 324.818 kb on + strand, within MPMX20_00303 at 324.818 kb on + strand, within MPMX20_00303 at 324.818 kb on + strand, within MPMX20_00303 at 324.819 kb on - strand, within MPMX20_00303 at 324.819 kb on - strand, within MPMX20_00303 at 324.826 kb on - strand, within MPMX20_00303 at 324.826 kb on - strand, within MPMX20_00303 at 324.826 kb on - strand, within MPMX20_00303 at 324.835 kb on + strand, within MPMX20_00303 at 324.835 kb on + strand, within MPMX20_00303 at 324.835 kb on + strand, within MPMX20_00303 at 324.843 kb on - strand, within MPMX20_00303 at 324.843 kb on - strand, within MPMX20_00303 at 324.843 kb on - strand, within MPMX20_00303 at 324.844 kb on + strand, within MPMX20_00303 at 324.868 kb on + strand, within MPMX20_00303 at 324.900 kb on + strand, within MPMX20_00303 at 324.903 kb on - strand, within MPMX20_00303 at 324.931 kb on - strand, within MPMX20_00303 at 325.082 kb on - strand, within MPMX20_00303 at 325.128 kb on + strand, within MPMX20_00303 at 325.128 kb on + strand, within MPMX20_00303 at 325.136 kb on - strand, within MPMX20_00303 at 325.188 kb on + strand, within MPMX20_00303 at 325.189 kb on - strand, within MPMX20_00303 at 325.214 kb on - strand, within MPMX20_00303 at 325.270 kb on - strand, within MPMX20_00303 at 325.270 kb on - strand, within MPMX20_00303 at 325.270 kb on - strand, within MPMX20_00303 at 325.284 kb on - strand, within MPMX20_00303 at 325.379 kb on + strand, within MPMX20_00303 at 325.396 kb on + strand, within MPMX20_00303 at 325.422 kb on + strand, within MPMX20_00303 at 325.465 kb on - strand, within MPMX20_00303 at 325.598 kb on + strand, within MPMX20_00303 at 325.598 kb on + strand, within MPMX20_00303 at 325.606 kb on - strand, within MPMX20_00303 at 325.606 kb on - strand, within MPMX20_00303 at 325.638 kb on + strand, within MPMX20_00303 at 325.646 kb on - strand, within MPMX20_00303 at 325.712 kb on - strand, within MPMX20_00303 at 325.748 kb on + strand, within MPMX20_00303 at 325.748 kb on + strand, within MPMX20_00303 at 325.755 kb on - strand, within MPMX20_00303 at 325.756 kb on - strand, within MPMX20_00303 at 325.756 kb on - strand, within MPMX20_00303 at 325.756 kb on - strand, within MPMX20_00303 at 325.765 kb on + strand, within MPMX20_00303 at 325.821 kb on - strand, within MPMX20_00303 at 325.822 kb on - strand, within MPMX20_00303 at 325.861 kb on - strand, within MPMX20_00303 at 325.879 kb on + strand, within MPMX20_00303 at 325.879 kb on + strand, within MPMX20_00303 at 325.963 kb on - strand, within MPMX20_00303 at 326.020 kb on - strand, within MPMX20_00303 at 326.072 kb on + strand, within MPMX20_00303 at 326.080 kb on - strand, within MPMX20_00303 at 326.080 kb on - strand, within MPMX20_00303 at 326.080 kb on - strand, within MPMX20_00303 at 326.091 kb on + strand at 326.091 kb on + strand at 326.099 kb on + strand at 326.107 kb on - strand at 326.114 kb on + strand at 326.115 kb on - strand at 326.117 kb on - strand at 326.134 kb on - strand at 326.134 kb on - strand at 326.214 kb on + strand at 326.214 kb on - strand at 326.221 kb on + strand at 326.229 kb on - strand at 326.229 kb on - strand at 326.231 kb on + strand at 326.299 kb on + strand at 326.305 kb on - strand at 326.305 kb on - strand at 326.307 kb on + strand at 326.315 kb on - strand at 326.346 kb on + strand, within MPMX20_00304 at 326.357 kb on - strand, within MPMX20_00304 at 326.364 kb on - strand, within MPMX20_00304 at 326.375 kb on - strand, within MPMX20_00304 at 326.464 kb on + strand, within MPMX20_00304 at 326.474 kb on + strand, within MPMX20_00304 at 326.482 kb on - strand, within MPMX20_00304 at 326.483 kb on + strand, within MPMX20_00304 at 326.519 kb on + strand, within MPMX20_00304 at 326.529 kb on - strand, within MPMX20_00304 at 326.545 kb on - strand, within MPMX20_00304 at 326.601 kb on - strand, within MPMX20_00304 at 326.628 kb on + strand, within MPMX20_00304 at 326.655 kb on + strand, within MPMX20_00304 at 326.661 kb on - strand, within MPMX20_00304 at 326.696 kb on + strand, within MPMX20_00304 at 326.706 kb on + strand, within MPMX20_00304 at 326.713 kb on - strand, within MPMX20_00304 at 326.714 kb on - strand, within MPMX20_00304 at 326.714 kb on - strand, within MPMX20_00304 at 326.738 kb on + strand, within MPMX20_00304 at 326.808 kb on - strand, within MPMX20_00304 at 326.808 kb on - strand, within MPMX20_00304 at 326.928 kb on - strand, within MPMX20_00304 at 326.928 kb on - strand, within MPMX20_00304 at 326.950 kb on + strand, within MPMX20_00304 at 326.950 kb on + strand, within MPMX20_00304 at 327.009 kb on - strand, within MPMX20_00304 at 327.009 kb on - strand, within MPMX20_00304 at 327.032 kb on + strand, within MPMX20_00304 at 327.032 kb on + strand, within MPMX20_00304 at 327.040 kb on - strand, within MPMX20_00304 at 327.075 kb on - strand at 327.077 kb on + strand at 327.085 kb on - strand at 327.085 kb on - strand at 327.089 kb on + strand at 327.096 kb on + strand at 327.096 kb on + strand at 327.104 kb on - strand at 327.114 kb on - strand at 327.116 kb on + strand at 327.137 kb on - strand at 327.141 kb on - strand at 327.178 kb on + strand at 327.249 kb on + strand, within MPMX20_00305 at 327.252 kb on - strand, within MPMX20_00305 at 327.254 kb on + strand, within MPMX20_00305 at 327.254 kb on + strand, within MPMX20_00305 at 327.254 kb on + strand, within MPMX20_00305 at 327.257 kb on - strand, within MPMX20_00305 at 327.262 kb on - strand, within MPMX20_00305 at 327.262 kb on - strand, within MPMX20_00305
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, Mixed culture; Bacillus subtilis TBS_102 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 322,992 + MPMX20_00302 0.46 +0.5 322,993 - MPMX20_00302 0.46 -0.9 322,993 - MPMX20_00302 0.46 +0.5 322,995 - MPMX20_00302 0.46 -1.2 322,995 - MPMX20_00302 0.46 +0.6 323,027 - MPMX20_00302 0.48 -0.9 323,035 - MPMX20_00302 0.49 +0.5 323,056 - MPMX20_00302 0.50 -2.3 323,065 + MPMX20_00302 0.51 +0.3 323,065 + MPMX20_00302 0.51 -1.7 323,065 + MPMX20_00302 0.51 +1.0 323,111 + MPMX20_00302 0.54 +1.3 323,111 + MPMX20_00302 0.54 +0.8 323,111 + MPMX20_00302 0.54 +5.6 323,119 - MPMX20_00302 0.55 -1.3 323,119 - MPMX20_00302 0.55 -1.0 323,119 - MPMX20_00302 0.55 -1.3 323,119 - MPMX20_00302 0.55 -2.0 323,119 - MPMX20_00302 0.55 +3.3 323,159 - MPMX20_00302 0.57 +0.6 323,270 + MPMX20_00302 0.64 -0.5 323,284 + MPMX20_00302 0.65 +3.4 323,292 - MPMX20_00302 0.66 -1.4 323,292 - MPMX20_00302 0.66 -2.5 323,302 - MPMX20_00302 0.67 +1.6 323,340 - MPMX20_00302 0.69 +2.6 323,374 + MPMX20_00302 0.71 -2.1 323,382 - MPMX20_00302 0.72 -0.9 323,382 - MPMX20_00302 0.72 +1.6 323,384 + MPMX20_00302 0.72 -2.5 323,404 + MPMX20_00302 0.73 -0.1 323,412 - MPMX20_00302 0.74 +0.6 323,412 - MPMX20_00302 0.74 -0.5 323,468 + MPMX20_00302 0.77 -0.8 323,472 - MPMX20_00302 0.78 -1.8 323,476 - MPMX20_00302 0.78 -1.3 323,533 - MPMX20_00302 0.82 +0.0 323,649 - MPMX20_00302 0.89 -0.2 323,673 + -1.8 323,686 - -0.3 323,688 + -1.1 323,733 + +1.7 323,740 + +2.6 323,748 - -2.9 323,748 - -1.6 323,795 - +0.6 323,849 - -2.2 323,895 + -0.8 323,895 + +0.0 323,903 - +0.3 323,903 - +0.3 323,941 + +1.0 323,941 + +1.6 323,949 - +0.3 323,951 + -1.8 323,960 - +0.2 323,963 + +1.0 323,963 + -0.2 323,965 - -0.4 323,979 - +0.1 323,995 + -1.0 324,086 - +1.2 324,094 - -0.3 324,094 - +0.8 324,175 - -1.1 324,196 - +0.5 324,219 + -0.1 324,296 + MPMX20_00303 0.13 -0.1 324,304 - MPMX20_00303 0.13 +1.0 324,338 - MPMX20_00303 0.15 -1.5 324,338 - MPMX20_00303 0.15 +0.8 324,374 + MPMX20_00303 0.16 -1.0 324,374 + MPMX20_00303 0.16 -0.3 324,386 + MPMX20_00303 0.17 +2.6 324,421 + MPMX20_00303 0.18 +1.6 324,439 - MPMX20_00303 0.19 +1.6 324,439 - MPMX20_00303 0.19 -1.3 324,455 + MPMX20_00303 0.20 +1.1 324,548 + MPMX20_00303 0.24 -1.4 324,622 - MPMX20_00303 0.27 +0.2 324,622 - MPMX20_00303 0.27 +0.0 324,632 + MPMX20_00303 0.28 -0.7 324,632 - MPMX20_00303 0.28 +0.4 324,632 - MPMX20_00303 0.28 +1.0 324,653 + MPMX20_00303 0.28 -0.4 324,689 + MPMX20_00303 0.30 -1.5 324,697 - MPMX20_00303 0.30 -1.0 324,697 - MPMX20_00303 0.30 -0.4 324,697 - MPMX20_00303 0.30 -1.4 324,702 - MPMX20_00303 0.31 -1.5 324,718 - MPMX20_00303 0.31 +1.0 324,772 + MPMX20_00303 0.34 -0.8 324,805 - MPMX20_00303 0.35 -0.1 324,818 + MPMX20_00303 0.36 -0.9 324,818 + MPMX20_00303 0.36 +0.4 324,818 + MPMX20_00303 0.36 +1.6 324,819 - MPMX20_00303 0.36 -0.5 324,819 - MPMX20_00303 0.36 -1.6 324,826 - MPMX20_00303 0.36 -1.8 324,826 - MPMX20_00303 0.36 +0.6 324,826 - MPMX20_00303 0.36 +1.6 324,835 + MPMX20_00303 0.36 +0.1 324,835 + MPMX20_00303 0.36 -1.5 324,835 + MPMX20_00303 0.36 -1.0 324,843 - MPMX20_00303 0.37 -1.4 324,843 - MPMX20_00303 0.37 -1.6 324,843 - MPMX20_00303 0.37 -0.8 324,844 + MPMX20_00303 0.37 -1.3 324,868 + MPMX20_00303 0.38 -1.0 324,900 + MPMX20_00303 0.39 -1.4 324,903 - MPMX20_00303 0.39 -0.5 324,931 - MPMX20_00303 0.40 -1.0 325,082 - MPMX20_00303 0.47 +0.0 325,128 + MPMX20_00303 0.49 -1.1 325,128 + MPMX20_00303 0.49 -0.5 325,136 - MPMX20_00303 0.49 -1.2 325,188 + MPMX20_00303 0.52 -1.2 325,189 - MPMX20_00303 0.52 -0.7 325,214 - MPMX20_00303 0.53 -0.4 325,270 - MPMX20_00303 0.55 -0.8 325,270 - MPMX20_00303 0.55 -1.0 325,270 - MPMX20_00303 0.55 -1.4 325,284 - MPMX20_00303 0.56 +0.6 325,379 + MPMX20_00303 0.60 +3.1 325,396 + MPMX20_00303 0.60 -0.2 325,422 + MPMX20_00303 0.62 +0.3 325,465 - MPMX20_00303 0.63 -0.0 325,598 + MPMX20_00303 0.69 -1.3 325,598 + MPMX20_00303 0.69 +1.4 325,606 - MPMX20_00303 0.70 +1.3 325,606 - MPMX20_00303 0.70 +0.2 325,638 + MPMX20_00303 0.71 -0.7 325,646 - MPMX20_00303 0.71 +1.3 325,712 - MPMX20_00303 0.74 +1.0 325,748 + MPMX20_00303 0.76 +0.2 325,748 + MPMX20_00303 0.76 +2.6 325,755 - MPMX20_00303 0.76 -0.2 325,756 - MPMX20_00303 0.76 -2.8 325,756 - MPMX20_00303 0.76 -0.7 325,756 - MPMX20_00303 0.76 -0.1 325,765 + MPMX20_00303 0.76 +0.6 325,821 - MPMX20_00303 0.79 -0.8 325,822 - MPMX20_00303 0.79 -0.6 325,861 - MPMX20_00303 0.80 -1.2 325,879 + MPMX20_00303 0.81 -0.2 325,879 + MPMX20_00303 0.81 -0.6 325,963 - MPMX20_00303 0.85 +0.0 326,020 - MPMX20_00303 0.87 +0.6 326,072 + MPMX20_00303 0.90 -1.7 326,080 - MPMX20_00303 0.90 -2.0 326,080 - MPMX20_00303 0.90 -0.7 326,080 - MPMX20_00303 0.90 -2.0 326,091 + -0.4 326,091 + -1.1 326,099 + -1.2 326,107 - -0.1 326,114 + -0.2 326,115 - -2.1 326,117 - +0.1 326,134 - -1.1 326,134 - -0.7 326,214 + +2.0 326,214 - +1.9 326,221 + +0.9 326,229 - -0.2 326,229 - -2.7 326,231 + +1.9 326,299 + -0.8 326,305 - +1.2 326,305 - -1.8 326,307 + +0.2 326,315 - +0.6 326,346 + MPMX20_00304 0.13 -2.7 326,357 - MPMX20_00304 0.14 -0.7 326,364 - MPMX20_00304 0.15 -1.1 326,375 - MPMX20_00304 0.16 -2.0 326,464 + MPMX20_00304 0.25 +0.6 326,474 + MPMX20_00304 0.26 -2.1 326,482 - MPMX20_00304 0.27 -1.3 326,483 + MPMX20_00304 0.27 +0.3 326,519 + MPMX20_00304 0.31 +2.6 326,529 - MPMX20_00304 0.32 -0.8 326,545 - MPMX20_00304 0.34 +1.0 326,601 - MPMX20_00304 0.40 +1.0 326,628 + MPMX20_00304 0.43 -0.7 326,655 + MPMX20_00304 0.46 +0.5 326,661 - MPMX20_00304 0.46 -0.2 326,696 + MPMX20_00304 0.50 +0.0 326,706 + MPMX20_00304 0.51 -0.4 326,713 - MPMX20_00304 0.52 -2.2 326,714 - MPMX20_00304 0.52 +9.0 326,714 - MPMX20_00304 0.52 -1.8 326,738 + MPMX20_00304 0.54 -1.1 326,808 - MPMX20_00304 0.62 -0.6 326,808 - MPMX20_00304 0.62 -0.4 326,928 - MPMX20_00304 0.74 -1.4 326,928 - MPMX20_00304 0.74 +2.9 326,950 + MPMX20_00304 0.77 -0.4 326,950 + MPMX20_00304 0.77 +0.0 327,009 - MPMX20_00304 0.83 -0.4 327,009 - MPMX20_00304 0.83 -2.3 327,032 + MPMX20_00304 0.85 -1.1 327,032 + MPMX20_00304 0.85 -0.2 327,040 - MPMX20_00304 0.86 +0.0 327,075 - -0.4 327,077 + -2.2 327,085 - -0.7 327,085 - -2.6 327,089 + -0.3 327,096 + +1.6 327,096 + -0.8 327,104 - -1.0 327,114 - -2.6 327,116 + -1.4 327,137 - -0.3 327,141 - +0.1 327,178 + +0.2 327,249 + MPMX20_00305 0.14 -0.8 327,252 - MPMX20_00305 0.14 -0.4 327,254 + MPMX20_00305 0.15 -0.7 327,254 + MPMX20_00305 0.15 +0.2 327,254 + MPMX20_00305 0.15 -1.3 327,257 - MPMX20_00305 0.15 -1.0 327,262 - MPMX20_00305 0.16 +1.1 327,262 - MPMX20_00305 0.16 +0.1
Or see this region's nucleotide sequence