Experiment: TSB plate, Mixed culture; Bacillus megaterium TBS_053 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00289 and MPMX20_00290 overlap by 4 nucleotides MPMX20_00290 and MPMX20_00291 are separated by 70 nucleotides MPMX20_00291 and MPMX20_00292 are separated by 423 nucleotides
MPMX20_00289: MPMX20_00289 - Cation/acetate symporter ActP, at 307,747 to 309,396
_00289
MPMX20_00290: MPMX20_00290 - Inner membrane protein YjcH, at 309,393 to 309,707
_00290
MPMX20_00291: MPMX20_00291 - Acetyl-coenzyme A synthetase, at 309,778 to 311,736
_00291
MPMX20_00292: MPMX20_00292 - Proton/glutamate-aspartate symporter, at 312,160 to 313,473
_00292
Position (kb) 309
310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 308.790 kb on - strand, within MPMX20_00289 at 308.798 kb on + strand, within MPMX20_00289 at 308.820 kb on - strand, within MPMX20_00289 at 308.856 kb on + strand, within MPMX20_00289 at 308.917 kb on - strand, within MPMX20_00289 at 308.917 kb on - strand, within MPMX20_00289 at 308.928 kb on + strand, within MPMX20_00289 at 308.952 kb on - strand, within MPMX20_00289 at 308.952 kb on - strand, within MPMX20_00289 at 308.970 kb on + strand, within MPMX20_00289 at 308.970 kb on + strand, within MPMX20_00289 at 309.009 kb on + strand, within MPMX20_00289 at 309.058 kb on + strand, within MPMX20_00289 at 309.066 kb on - strand, within MPMX20_00289 at 309.099 kb on - strand, within MPMX20_00289 at 309.104 kb on + strand, within MPMX20_00289 at 309.104 kb on + strand, within MPMX20_00289 at 309.104 kb on + strand, within MPMX20_00289 at 309.104 kb on + strand, within MPMX20_00289 at 309.111 kb on - strand, within MPMX20_00289 at 309.111 kb on - strand, within MPMX20_00289 at 309.112 kb on - strand, within MPMX20_00289 at 309.112 kb on - strand, within MPMX20_00289 at 309.112 kb on - strand, within MPMX20_00289 at 309.112 kb on - strand, within MPMX20_00289 at 309.112 kb on - strand, within MPMX20_00289 at 309.203 kb on + strand, within MPMX20_00289 at 309.203 kb on + strand, within MPMX20_00289 at 309.213 kb on + strand, within MPMX20_00289 at 309.256 kb on - strand at 309.258 kb on - strand at 309.318 kb on + strand at 309.371 kb on - strand at 309.389 kb on - strand at 309.408 kb on - strand at 309.411 kb on + strand at 309.421 kb on + strand at 309.429 kb on - strand, within MPMX20_00290 at 309.461 kb on + strand, within MPMX20_00290 at 309.479 kb on - strand, within MPMX20_00290 at 309.505 kb on - strand, within MPMX20_00290 at 309.561 kb on + strand, within MPMX20_00290 at 309.563 kb on - strand, within MPMX20_00290 at 309.569 kb on - strand, within MPMX20_00290 at 309.574 kb on - strand, within MPMX20_00290 at 309.579 kb on + strand, within MPMX20_00290 at 309.584 kb on + strand, within MPMX20_00290 at 309.584 kb on + strand, within MPMX20_00290 at 309.584 kb on + strand, within MPMX20_00290 at 309.592 kb on - strand, within MPMX20_00290 at 309.592 kb on - strand, within MPMX20_00290 at 309.656 kb on + strand, within MPMX20_00290 at 309.665 kb on + strand, within MPMX20_00290 at 309.666 kb on - strand, within MPMX20_00290 at 309.678 kb on + strand at 309.713 kb on - strand at 309.713 kb on - strand at 309.754 kb on + strand at 309.762 kb on - strand at 309.799 kb on - strand at 309.799 kb on - strand at 309.799 kb on - strand at 309.845 kb on - strand at 309.851 kb on - strand at 309.862 kb on - strand at 309.901 kb on + strand at 309.937 kb on + strand at 309.949 kb on - strand at 309.949 kb on - strand at 309.954 kb on - strand at 310.010 kb on - strand, within MPMX20_00291 at 310.014 kb on + strand, within MPMX20_00291 at 310.014 kb on + strand, within MPMX20_00291 at 310.019 kb on + strand, within MPMX20_00291 at 310.022 kb on - strand, within MPMX20_00291 at 310.034 kb on - strand, within MPMX20_00291 at 310.174 kb on + strand, within MPMX20_00291 at 310.284 kb on - strand, within MPMX20_00291 at 310.289 kb on - strand, within MPMX20_00291 at 310.401 kb on + strand, within MPMX20_00291 at 310.401 kb on + strand, within MPMX20_00291 at 310.425 kb on - strand, within MPMX20_00291 at 310.425 kb on - strand, within MPMX20_00291 at 310.436 kb on + strand, within MPMX20_00291 at 310.436 kb on + strand, within MPMX20_00291 at 310.436 kb on + strand, within MPMX20_00291 at 310.436 kb on + strand, within MPMX20_00291 at 310.444 kb on - strand, within MPMX20_00291 at 310.458 kb on + strand, within MPMX20_00291 at 310.458 kb on + strand, within MPMX20_00291 at 310.458 kb on + strand, within MPMX20_00291 at 310.458 kb on + strand, within MPMX20_00291 at 310.466 kb on - strand, within MPMX20_00291 at 310.466 kb on - strand, within MPMX20_00291 at 310.466 kb on - strand, within MPMX20_00291 at 310.466 kb on - strand, within MPMX20_00291 at 310.478 kb on - strand, within MPMX20_00291 at 310.494 kb on + strand, within MPMX20_00291 at 310.494 kb on + strand, within MPMX20_00291 at 310.494 kb on + strand, within MPMX20_00291 at 310.494 kb on + strand, within MPMX20_00291 at 310.494 kb on + strand, within MPMX20_00291 at 310.502 kb on - strand, within MPMX20_00291 at 310.502 kb on - strand, within MPMX20_00291 at 310.659 kb on + strand, within MPMX20_00291 at 310.687 kb on - strand, within MPMX20_00291 at 310.722 kb on + strand, within MPMX20_00291 at 310.722 kb on + strand, within MPMX20_00291 at 310.732 kb on + strand, within MPMX20_00291 at 310.750 kb on + strand, within MPMX20_00291 at 310.750 kb on + strand, within MPMX20_00291 at 310.789 kb on - strand, within MPMX20_00291 at 310.883 kb on + strand, within MPMX20_00291 at 310.890 kb on + strand, within MPMX20_00291 at 311.032 kb on - strand, within MPMX20_00291 at 311.042 kb on - strand, within MPMX20_00291 at 311.107 kb on - strand, within MPMX20_00291 at 311.284 kb on + strand, within MPMX20_00291 at 311.284 kb on + strand, within MPMX20_00291 at 311.365 kb on - strand, within MPMX20_00291 at 311.422 kb on + strand, within MPMX20_00291 at 311.453 kb on - strand, within MPMX20_00291 at 311.457 kb on - strand, within MPMX20_00291 at 311.468 kb on - strand, within MPMX20_00291 at 311.487 kb on + strand, within MPMX20_00291 at 311.487 kb on + strand, within MPMX20_00291 at 311.495 kb on - strand, within MPMX20_00291 at 311.495 kb on - strand, within MPMX20_00291 at 311.561 kb on - strand at 311.566 kb on + strand at 311.585 kb on + strand at 311.585 kb on + strand at 311.586 kb on + strand at 311.593 kb on - strand at 311.593 kb on - strand at 311.596 kb on + strand at 311.616 kb on - strand at 311.618 kb on + strand at 311.621 kb on - strand at 311.626 kb on - strand at 311.626 kb on - strand at 311.628 kb on + strand at 311.666 kb on - strand at 311.668 kb on - strand at 311.670 kb on + strand at 311.678 kb on - strand at 311.678 kb on - strand at 311.678 kb on - strand at 311.719 kb on + strand at 311.739 kb on - strand at 311.760 kb on - strand at 311.856 kb on - strand at 311.965 kb on + strand at 312.005 kb on + strand at 312.068 kb on + strand at 312.083 kb on + strand at 312.177 kb on - strand at 312.182 kb on - strand at 312.191 kb on - strand at 312.196 kb on - strand at 312.203 kb on + strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.211 kb on - strand at 312.223 kb on - strand at 312.227 kb on + strand at 312.279 kb on + strand at 312.279 kb on + strand at 312.282 kb on - strand at 312.287 kb on - strand at 312.287 kb on - strand at 312.287 kb on - strand at 312.349 kb on - strand, within MPMX20_00292 at 312.367 kb on + strand, within MPMX20_00292 at 312.385 kb on + strand, within MPMX20_00292 at 312.385 kb on + strand, within MPMX20_00292 at 312.473 kb on - strand, within MPMX20_00292 at 312.561 kb on - strand, within MPMX20_00292 at 312.561 kb on - strand, within MPMX20_00292 at 312.561 kb on - strand, within MPMX20_00292 at 312.576 kb on + strand, within MPMX20_00292 at 312.584 kb on - strand, within MPMX20_00292 at 312.631 kb on + strand, within MPMX20_00292 at 312.709 kb on + strand, within MPMX20_00292
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, Mixed culture; Bacillus megaterium TBS_053 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 308,790 - MPMX20_00289 0.63 +1.6 308,798 + MPMX20_00289 0.64 -1.4 308,820 - MPMX20_00289 0.65 -1.1 308,856 + MPMX20_00289 0.67 +0.8 308,917 - MPMX20_00289 0.71 -2.7 308,917 - MPMX20_00289 0.71 -1.5 308,928 + MPMX20_00289 0.72 -1.2 308,952 - MPMX20_00289 0.73 +0.4 308,952 - MPMX20_00289 0.73 -0.3 308,970 + MPMX20_00289 0.74 -1.0 308,970 + MPMX20_00289 0.74 -1.3 309,009 + MPMX20_00289 0.76 -0.8 309,058 + MPMX20_00289 0.79 -0.6 309,066 - MPMX20_00289 0.80 -1.6 309,099 - MPMX20_00289 0.82 -1.7 309,104 + MPMX20_00289 0.82 -0.6 309,104 + MPMX20_00289 0.82 -1.6 309,104 + MPMX20_00289 0.82 -0.5 309,104 + MPMX20_00289 0.82 -0.8 309,111 - MPMX20_00289 0.83 +0.2 309,111 - MPMX20_00289 0.83 -1.7 309,112 - MPMX20_00289 0.83 -0.8 309,112 - MPMX20_00289 0.83 -0.3 309,112 - MPMX20_00289 0.83 -1.8 309,112 - MPMX20_00289 0.83 -0.8 309,112 - MPMX20_00289 0.83 -3.0 309,203 + MPMX20_00289 0.88 -1.1 309,203 + MPMX20_00289 0.88 -1.7 309,213 + MPMX20_00289 0.89 -0.9 309,256 - +2.2 309,258 - +0.7 309,318 + -0.9 309,371 - +0.4 309,389 - +0.4 309,408 - +0.0 309,411 + -0.5 309,421 + +1.2 309,429 - MPMX20_00290 0.11 -2.1 309,461 + MPMX20_00290 0.22 -2.2 309,479 - MPMX20_00290 0.27 -0.8 309,505 - MPMX20_00290 0.36 -0.3 309,561 + MPMX20_00290 0.53 +0.7 309,563 - MPMX20_00290 0.54 -0.8 309,569 - MPMX20_00290 0.56 -1.1 309,574 - MPMX20_00290 0.57 +0.7 309,579 + MPMX20_00290 0.59 -1.5 309,584 + MPMX20_00290 0.61 -1.1 309,584 + MPMX20_00290 0.61 -2.2 309,584 + MPMX20_00290 0.61 -1.2 309,592 - MPMX20_00290 0.63 -0.8 309,592 - MPMX20_00290 0.63 -0.1 309,656 + MPMX20_00290 0.83 -0.1 309,665 + MPMX20_00290 0.86 +0.2 309,666 - MPMX20_00290 0.87 -0.0 309,678 + -0.3 309,713 - -1.1 309,713 - -0.5 309,754 + -0.4 309,762 - +0.2 309,799 - -1.0 309,799 - -1.3 309,799 - -1.2 309,845 - -0.1 309,851 - +0.4 309,862 - -0.3 309,901 + -1.6 309,937 + -0.4 309,949 - -1.7 309,949 - -1.6 309,954 - +1.7 310,010 - MPMX20_00291 0.12 -0.3 310,014 + MPMX20_00291 0.12 +1.2 310,014 + MPMX20_00291 0.12 -1.4 310,019 + MPMX20_00291 0.12 -1.6 310,022 - MPMX20_00291 0.12 +1.2 310,034 - MPMX20_00291 0.13 +0.8 310,174 + MPMX20_00291 0.20 +0.2 310,284 - MPMX20_00291 0.26 -0.5 310,289 - MPMX20_00291 0.26 -1.1 310,401 + MPMX20_00291 0.32 -2.6 310,401 + MPMX20_00291 0.32 -1.3 310,425 - MPMX20_00291 0.33 -0.8 310,425 - MPMX20_00291 0.33 -0.7 310,436 + MPMX20_00291 0.34 +0.2 310,436 + MPMX20_00291 0.34 -1.8 310,436 + MPMX20_00291 0.34 -0.3 310,436 + MPMX20_00291 0.34 -0.3 310,444 - MPMX20_00291 0.34 -1.6 310,458 + MPMX20_00291 0.35 -2.5 310,458 + MPMX20_00291 0.35 -0.1 310,458 + MPMX20_00291 0.35 -1.1 310,458 + MPMX20_00291 0.35 +1.4 310,466 - MPMX20_00291 0.35 +1.2 310,466 - MPMX20_00291 0.35 -1.1 310,466 - MPMX20_00291 0.35 -1.0 310,466 - MPMX20_00291 0.35 -2.3 310,478 - MPMX20_00291 0.36 -1.2 310,494 + MPMX20_00291 0.37 -2.2 310,494 + MPMX20_00291 0.37 -1.8 310,494 + MPMX20_00291 0.37 -0.9 310,494 + MPMX20_00291 0.37 -1.9 310,494 + MPMX20_00291 0.37 -1.8 310,502 - MPMX20_00291 0.37 -0.0 310,502 - MPMX20_00291 0.37 -1.3 310,659 + MPMX20_00291 0.45 -1.1 310,687 - MPMX20_00291 0.46 +0.5 310,722 + MPMX20_00291 0.48 -1.3 310,722 + MPMX20_00291 0.48 -0.1 310,732 + MPMX20_00291 0.49 -2.2 310,750 + MPMX20_00291 0.50 -0.1 310,750 + MPMX20_00291 0.50 -1.9 310,789 - MPMX20_00291 0.52 -0.8 310,883 + MPMX20_00291 0.56 -1.3 310,890 + MPMX20_00291 0.57 +0.0 311,032 - MPMX20_00291 0.64 -0.8 311,042 - MPMX20_00291 0.65 +1.2 311,107 - MPMX20_00291 0.68 +1.2 311,284 + MPMX20_00291 0.77 +1.2 311,284 + MPMX20_00291 0.77 -0.2 311,365 - MPMX20_00291 0.81 +0.7 311,422 + MPMX20_00291 0.84 +0.2 311,453 - MPMX20_00291 0.86 +0.5 311,457 - MPMX20_00291 0.86 -0.5 311,468 - MPMX20_00291 0.86 -0.8 311,487 + MPMX20_00291 0.87 -0.3 311,487 + MPMX20_00291 0.87 +0.5 311,495 - MPMX20_00291 0.88 -0.7 311,495 - MPMX20_00291 0.88 -1.3 311,561 - +1.2 311,566 + -0.2 311,585 + +0.5 311,585 + -0.2 311,586 + -1.5 311,593 - -1.2 311,593 - -0.2 311,596 + -0.7 311,616 - -1.3 311,618 + +1.0 311,621 - -0.3 311,626 - -0.4 311,626 - -1.9 311,628 + +1.2 311,666 - -0.7 311,668 - +1.2 311,670 + -1.5 311,678 - +1.7 311,678 - +0.7 311,678 - -1.2 311,719 + -0.9 311,739 - -0.8 311,760 - -0.7 311,856 - +0.0 311,965 + -0.2 312,005 + +0.4 312,068 + -0.2 312,083 + -0.3 312,177 - -0.1 312,182 - +1.0 312,191 - +0.8 312,196 - -0.3 312,203 + -1.9 312,211 - -0.9 312,211 - -0.8 312,211 - -0.1 312,211 - -0.5 312,211 - -0.7 312,211 - -0.2 312,211 - -0.2 312,211 - +1.0 312,211 - +0.5 312,211 - +0.1 312,223 - +0.7 312,227 + +0.7 312,279 + -0.7 312,279 + -0.3 312,282 - +0.7 312,287 - -0.9 312,287 - -0.3 312,287 - +0.2 312,349 - MPMX20_00292 0.14 -0.8 312,367 + MPMX20_00292 0.16 -0.9 312,385 + MPMX20_00292 0.17 -0.1 312,385 + MPMX20_00292 0.17 -0.8 312,473 - MPMX20_00292 0.24 -0.2 312,561 - MPMX20_00292 0.31 +0.2 312,561 - MPMX20_00292 0.31 -0.4 312,561 - MPMX20_00292 0.31 -0.3 312,576 + MPMX20_00292 0.32 -1.3 312,584 - MPMX20_00292 0.32 +3.0 312,631 + MPMX20_00292 0.36 +0.6 312,709 + MPMX20_00292 0.42 +1.2
Or see this region's nucleotide sequence