Experiment: M9 plate, Mixed culture; Bacillus megaterium TBS_053 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00815 and MPMX20_00816 are separated by 116 nucleotides MPMX20_00816 and MPMX20_00817 are separated by 67 nucleotides MPMX20_00817 and MPMX20_00818 are separated by 96 nucleotides
MPMX20_00815: MPMX20_00815 - Polyamine aminopropyltransferase, at 857,318 to 858,187
_00815
MPMX20_00816: MPMX20_00816 - hypothetical protein, at 858,304 to 858,690
_00816
MPMX20_00817: MPMX20_00817 - Blue copper oxidase CueO, at 858,758 to 860,356
_00817
MPMX20_00818: MPMX20_00818 - Quinoprotein glucose dehydrogenase, at 860,453 to 862,843
_00818
Position (kb) 858
859
860
861 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 857.765 kb on - strand, within MPMX20_00815 at 857.811 kb on + strand, within MPMX20_00815 at 857.811 kb on + strand, within MPMX20_00815 at 857.819 kb on - strand, within MPMX20_00815 at 857.828 kb on + strand, within MPMX20_00815 at 857.828 kb on + strand, within MPMX20_00815 at 857.914 kb on + strand, within MPMX20_00815 at 857.922 kb on - strand, within MPMX20_00815 at 857.922 kb on - strand, within MPMX20_00815 at 857.948 kb on + strand, within MPMX20_00815 at 857.974 kb on + strand, within MPMX20_00815 at 858.119 kb on - strand at 858.142 kb on - strand at 858.142 kb on - strand at 858.142 kb on - strand at 858.197 kb on + strand at 858.259 kb on - strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.272 kb on + strand at 858.280 kb on - strand at 858.280 kb on - strand at 858.280 kb on - strand at 858.280 kb on - strand at 858.280 kb on - strand at 858.280 kb on - strand at 858.280 kb on - strand at 858.282 kb on + strand at 858.284 kb on + strand at 858.284 kb on + strand at 858.284 kb on + strand at 858.340 kb on + strand at 858.543 kb on - strand, within MPMX20_00816 at 858.545 kb on + strand, within MPMX20_00816 at 858.564 kb on - strand, within MPMX20_00816 at 858.565 kb on - strand, within MPMX20_00816 at 858.585 kb on + strand, within MPMX20_00816 at 858.585 kb on + strand, within MPMX20_00816 at 858.585 kb on + strand, within MPMX20_00816 at 858.593 kb on - strand, within MPMX20_00816 at 858.595 kb on - strand, within MPMX20_00816 at 858.661 kb on - strand at 858.700 kb on - strand at 858.700 kb on - strand at 858.857 kb on + strand at 858.865 kb on - strand at 858.918 kb on + strand, within MPMX20_00817 at 858.927 kb on + strand, within MPMX20_00817 at 858.928 kb on - strand, within MPMX20_00817 at 859.020 kb on + strand, within MPMX20_00817 at 859.028 kb on - strand, within MPMX20_00817 at 859.033 kb on - strand, within MPMX20_00817 at 859.045 kb on - strand, within MPMX20_00817 at 859.077 kb on + strand, within MPMX20_00817 at 859.101 kb on + strand, within MPMX20_00817 at 859.101 kb on + strand, within MPMX20_00817 at 859.106 kb on + strand, within MPMX20_00817 at 859.115 kb on + strand, within MPMX20_00817 at 859.115 kb on - strand, within MPMX20_00817 at 859.157 kb on - strand, within MPMX20_00817 at 859.187 kb on - strand, within MPMX20_00817 at 859.193 kb on - strand, within MPMX20_00817 at 859.218 kb on + strand, within MPMX20_00817 at 859.229 kb on - strand, within MPMX20_00817 at 859.319 kb on - strand, within MPMX20_00817 at 859.401 kb on - strand, within MPMX20_00817 at 859.436 kb on + strand, within MPMX20_00817 at 859.436 kb on - strand, within MPMX20_00817 at 859.483 kb on - strand, within MPMX20_00817 at 859.491 kb on + strand, within MPMX20_00817 at 859.499 kb on - strand, within MPMX20_00817 at 859.499 kb on - strand, within MPMX20_00817 at 859.504 kb on - strand, within MPMX20_00817 at 859.509 kb on - strand, within MPMX20_00817 at 859.611 kb on - strand, within MPMX20_00817 at 859.635 kb on + strand, within MPMX20_00817 at 859.643 kb on - strand, within MPMX20_00817 at 859.670 kb on + strand, within MPMX20_00817 at 859.681 kb on + strand, within MPMX20_00817 at 859.830 kb on + strand, within MPMX20_00817 at 859.871 kb on + strand, within MPMX20_00817 at 859.944 kb on - strand, within MPMX20_00817 at 860.179 kb on + strand, within MPMX20_00817 at 860.228 kb on + strand at 860.234 kb on - strand at 860.234 kb on - strand at 860.234 kb on - strand at 860.281 kb on - strand at 860.304 kb on + strand at 860.331 kb on - strand at 860.335 kb on + strand at 860.344 kb on - strand at 860.351 kb on + strand at 860.380 kb on + strand at 860.385 kb on + strand at 860.388 kb on - strand at 860.395 kb on - strand at 860.475 kb on + strand at 860.495 kb on - strand at 860.542 kb on - strand at 860.560 kb on + strand at 860.596 kb on + strand at 860.765 kb on + strand, within MPMX20_00818 at 860.765 kb on + strand, within MPMX20_00818 at 860.773 kb on - strand, within MPMX20_00818 at 860.773 kb on - strand, within MPMX20_00818 at 860.836 kb on - strand, within MPMX20_00818 at 860.837 kb on - strand, within MPMX20_00818 at 860.895 kb on + strand, within MPMX20_00818 at 860.895 kb on + strand, within MPMX20_00818 at 860.925 kb on - strand, within MPMX20_00818 at 860.929 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.952 kb on + strand, within MPMX20_00818 at 860.960 kb on - strand, within MPMX20_00818 at 860.960 kb on - strand, within MPMX20_00818 at 860.965 kb on + strand, within MPMX20_00818 at 860.973 kb on - strand, within MPMX20_00818 at 860.974 kb on + strand, within MPMX20_00818 at 860.983 kb on - strand, within MPMX20_00818 at 860.985 kb on + strand, within MPMX20_00818 at 861.042 kb on + strand, within MPMX20_00818 at 861.044 kb on + strand, within MPMX20_00818 at 861.110 kb on - strand, within MPMX20_00818 at 861.112 kb on + strand, within MPMX20_00818 at 861.120 kb on - strand, within MPMX20_00818 at 861.195 kb on + strand, within MPMX20_00818 at 861.203 kb on - strand, within MPMX20_00818 at 861.236 kb on + strand, within MPMX20_00818 at 861.249 kb on - strand, within MPMX20_00818 at 861.249 kb on - strand, within MPMX20_00818 at 861.330 kb on + strand, within MPMX20_00818
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, Mixed culture; Bacillus megaterium TBS_053 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 857,765 - MPMX20_00815 0.51 +0.0 857,811 + MPMX20_00815 0.57 +1.5 857,811 + MPMX20_00815 0.57 -1.2 857,819 - MPMX20_00815 0.58 +2.0 857,828 + MPMX20_00815 0.59 -0.7 857,828 + MPMX20_00815 0.59 -0.4 857,914 + MPMX20_00815 0.69 +0.7 857,922 - MPMX20_00815 0.69 +1.6 857,922 - MPMX20_00815 0.69 +0.6 857,948 + MPMX20_00815 0.72 +0.8 857,974 + MPMX20_00815 0.75 -0.0 858,119 - -0.0 858,142 - -0.7 858,142 - +0.4 858,142 - -1.0 858,197 + -0.7 858,259 - +0.3 858,272 + -1.4 858,272 + +0.6 858,272 + +1.6 858,272 + -0.7 858,272 + +2.4 858,272 + -1.3 858,272 + +0.3 858,272 + -0.8 858,272 + -0.8 858,280 - -0.2 858,280 - -0.7 858,280 - -2.8 858,280 - +0.0 858,280 - +1.8 858,280 - -0.1 858,280 - -0.2 858,282 + -1.5 858,284 + -2.6 858,284 + +0.2 858,284 + -2.8 858,340 + -0.2 858,543 - MPMX20_00816 0.62 -1.8 858,545 + MPMX20_00816 0.62 -1.2 858,564 - MPMX20_00816 0.67 -1.6 858,565 - MPMX20_00816 0.67 -0.7 858,585 + MPMX20_00816 0.73 +1.7 858,585 + MPMX20_00816 0.73 -1.1 858,585 + MPMX20_00816 0.73 -1.3 858,593 - MPMX20_00816 0.75 +0.4 858,595 - MPMX20_00816 0.75 -0.8 858,661 - +2.2 858,700 - -2.3 858,700 - -0.6 858,857 + -1.2 858,865 - +1.1 858,918 + MPMX20_00817 0.10 -0.7 858,927 + MPMX20_00817 0.11 +1.7 858,928 - MPMX20_00817 0.11 -0.4 859,020 + MPMX20_00817 0.16 -1.8 859,028 - MPMX20_00817 0.17 +0.5 859,033 - MPMX20_00817 0.17 -1.2 859,045 - MPMX20_00817 0.18 +0.1 859,077 + MPMX20_00817 0.20 -1.1 859,101 + MPMX20_00817 0.21 +1.1 859,101 + MPMX20_00817 0.21 -1.5 859,106 + MPMX20_00817 0.22 -1.2 859,115 + MPMX20_00817 0.22 -1.4 859,115 - MPMX20_00817 0.22 +0.5 859,157 - MPMX20_00817 0.25 -0.3 859,187 - MPMX20_00817 0.27 -1.6 859,193 - MPMX20_00817 0.27 -0.0 859,218 + MPMX20_00817 0.29 -1.2 859,229 - MPMX20_00817 0.29 -0.6 859,319 - MPMX20_00817 0.35 +0.1 859,401 - MPMX20_00817 0.40 +2.7 859,436 + MPMX20_00817 0.42 -0.8 859,436 - MPMX20_00817 0.42 +0.3 859,483 - MPMX20_00817 0.45 -0.2 859,491 + MPMX20_00817 0.46 -0.4 859,499 - MPMX20_00817 0.46 +0.3 859,499 - MPMX20_00817 0.46 +1.1 859,504 - MPMX20_00817 0.47 +8.6 859,509 - MPMX20_00817 0.47 +0.4 859,611 - MPMX20_00817 0.53 -1.6 859,635 + MPMX20_00817 0.55 +1.4 859,643 - MPMX20_00817 0.55 -0.1 859,670 + MPMX20_00817 0.57 -1.4 859,681 + MPMX20_00817 0.58 +1.8 859,830 + MPMX20_00817 0.67 -0.5 859,871 + MPMX20_00817 0.70 -1.0 859,944 - MPMX20_00817 0.74 -0.0 860,179 + MPMX20_00817 0.89 -1.6 860,228 + -0.4 860,234 - -0.2 860,234 - -0.2 860,234 - -0.7 860,281 - -0.7 860,304 + -2.1 860,331 - +0.8 860,335 + +2.9 860,344 - -0.7 860,351 + -0.3 860,380 + -0.2 860,385 + -0.4 860,388 - -0.5 860,395 - +0.8 860,475 + +6.2 860,495 - -0.9 860,542 - -0.4 860,560 + +0.6 860,596 + +1.3 860,765 + MPMX20_00818 0.13 -0.2 860,765 + MPMX20_00818 0.13 -1.7 860,773 - MPMX20_00818 0.13 -1.3 860,773 - MPMX20_00818 0.13 +1.6 860,836 - MPMX20_00818 0.16 +2.2 860,837 - MPMX20_00818 0.16 +0.4 860,895 + MPMX20_00818 0.18 +0.3 860,895 + MPMX20_00818 0.18 +1.1 860,925 - MPMX20_00818 0.20 +1.6 860,929 + MPMX20_00818 0.20 -1.4 860,952 + MPMX20_00818 0.21 -0.7 860,952 + MPMX20_00818 0.21 +0.8 860,952 + MPMX20_00818 0.21 +0.1 860,952 + MPMX20_00818 0.21 +0.8 860,952 + MPMX20_00818 0.21 +0.4 860,952 + MPMX20_00818 0.21 +0.6 860,952 + MPMX20_00818 0.21 -1.2 860,960 - MPMX20_00818 0.21 +1.0 860,960 - MPMX20_00818 0.21 +1.0 860,965 + MPMX20_00818 0.21 -2.5 860,973 - MPMX20_00818 0.22 -0.4 860,974 + MPMX20_00818 0.22 +0.5 860,983 - MPMX20_00818 0.22 +2.7 860,985 + MPMX20_00818 0.22 +0.3 861,042 + MPMX20_00818 0.25 -1.1 861,044 + MPMX20_00818 0.25 -0.3 861,110 - MPMX20_00818 0.27 -0.3 861,112 + MPMX20_00818 0.28 -1.9 861,120 - MPMX20_00818 0.28 +0.7 861,195 + MPMX20_00818 0.31 -0.6 861,203 - MPMX20_00818 0.31 -1.0 861,236 + MPMX20_00818 0.33 -0.8 861,249 - MPMX20_00818 0.33 -0.7 861,249 - MPMX20_00818 0.33 -1.0 861,330 + MPMX20_00818 0.37 -1.5
Or see this region's nucleotide sequence