Experiment: M9 plate, Mixed culture; Bacillus megaterium TBS_053 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00540 and MPMX20_00541 are separated by 150 nucleotides MPMX20_00541 and MPMX20_00542 are separated by 66 nucleotides MPMX20_00542 and MPMX20_00543 are separated by 74 nucleotides
MPMX20_00540: MPMX20_00540 - Magnesium-transporting ATPase, P-type 1, at 560,441 to 563,149
_00540
MPMX20_00541: MPMX20_00541 - hypothetical protein, at 563,300 to 565,684
_00541
MPMX20_00542: MPMX20_00542 - 2-iminobutanoate/2-iminopropanoate deaminase, at 565,751 to 566,137
_00542
MPMX20_00543: MPMX20_00543 - Aspartate carbamoyltransferase regulatory chain, at 566,212 to 566,673
_00543
Position (kb) 563
564
565
566 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 562.315 kb on - strand, within MPMX20_00540 at 562.344 kb on - strand, within MPMX20_00540 at 562.358 kb on - strand, within MPMX20_00540 at 562.389 kb on + strand, within MPMX20_00540 at 562.489 kb on - strand, within MPMX20_00540 at 562.631 kb on + strand, within MPMX20_00540 at 562.640 kb on - strand, within MPMX20_00540 at 562.728 kb on + strand, within MPMX20_00540 at 562.752 kb on + strand, within MPMX20_00540 at 562.760 kb on - strand, within MPMX20_00540 at 562.760 kb on - strand, within MPMX20_00540 at 562.765 kb on - strand, within MPMX20_00540 at 562.772 kb on - strand, within MPMX20_00540 at 562.793 kb on + strand, within MPMX20_00540 at 562.838 kb on + strand, within MPMX20_00540 at 562.863 kb on - strand, within MPMX20_00540 at 562.863 kb on - strand, within MPMX20_00540 at 562.870 kb on + strand, within MPMX20_00540 at 562.945 kb on - strand at 562.950 kb on - strand at 562.950 kb on - strand at 562.995 kb on + strand at 562.995 kb on + strand at 563.003 kb on - strand at 563.003 kb on - strand at 563.055 kb on + strand at 563.110 kb on + strand at 563.110 kb on + strand at 563.110 kb on + strand at 563.111 kb on + strand at 563.111 kb on + strand at 563.118 kb on - strand at 563.142 kb on - strand at 563.212 kb on - strand at 563.356 kb on - strand at 563.383 kb on + strand at 563.383 kb on + strand at 563.383 kb on + strand at 563.391 kb on - strand at 563.398 kb on + strand at 563.421 kb on + strand at 563.424 kb on + strand at 563.432 kb on - strand at 563.450 kb on - strand at 563.613 kb on + strand, within MPMX20_00541 at 563.704 kb on + strand, within MPMX20_00541 at 563.737 kb on - strand, within MPMX20_00541 at 563.824 kb on + strand, within MPMX20_00541 at 563.856 kb on - strand, within MPMX20_00541 at 563.928 kb on + strand, within MPMX20_00541 at 563.928 kb on + strand, within MPMX20_00541 at 563.936 kb on - strand, within MPMX20_00541 at 563.936 kb on - strand, within MPMX20_00541 at 563.962 kb on + strand, within MPMX20_00541 at 563.962 kb on + strand, within MPMX20_00541 at 563.962 kb on + strand, within MPMX20_00541 at 563.970 kb on - strand, within MPMX20_00541 at 563.970 kb on - strand, within MPMX20_00541 at 563.998 kb on - strand, within MPMX20_00541 at 564.000 kb on + strand, within MPMX20_00541 at 564.005 kb on + strand, within MPMX20_00541 at 564.008 kb on - strand, within MPMX20_00541 at 564.070 kb on + strand, within MPMX20_00541 at 564.083 kb on - strand, within MPMX20_00541 at 564.093 kb on + strand, within MPMX20_00541 at 564.093 kb on + strand, within MPMX20_00541 at 564.101 kb on - strand, within MPMX20_00541 at 564.101 kb on - strand, within MPMX20_00541 at 564.118 kb on + strand, within MPMX20_00541 at 564.118 kb on + strand, within MPMX20_00541 at 564.126 kb on - strand, within MPMX20_00541 at 564.286 kb on + strand, within MPMX20_00541 at 564.323 kb on - strand, within MPMX20_00541 at 564.331 kb on - strand, within MPMX20_00541 at 564.331 kb on - strand, within MPMX20_00541 at 564.370 kb on + strand, within MPMX20_00541 at 564.387 kb on + strand, within MPMX20_00541 at 564.436 kb on + strand, within MPMX20_00541 at 564.436 kb on + strand, within MPMX20_00541 at 564.443 kb on - strand, within MPMX20_00541 at 564.526 kb on + strand, within MPMX20_00541 at 564.534 kb on - strand, within MPMX20_00541 at 564.581 kb on + strand, within MPMX20_00541 at 564.613 kb on - strand, within MPMX20_00541 at 564.618 kb on - strand, within MPMX20_00541 at 564.660 kb on + strand, within MPMX20_00541 at 564.660 kb on + strand, within MPMX20_00541 at 564.668 kb on - strand, within MPMX20_00541 at 564.731 kb on + strand, within MPMX20_00541 at 564.739 kb on - strand, within MPMX20_00541 at 564.822 kb on + strand, within MPMX20_00541 at 564.822 kb on + strand, within MPMX20_00541 at 564.830 kb on - strand, within MPMX20_00541 at 564.830 kb on - strand, within MPMX20_00541 at 564.845 kb on - strand, within MPMX20_00541 at 564.895 kb on + strand, within MPMX20_00541 at 564.927 kb on + strand, within MPMX20_00541 at 564.935 kb on - strand, within MPMX20_00541 at 564.936 kb on + strand, within MPMX20_00541 at 565.020 kb on + strand, within MPMX20_00541 at 565.028 kb on - strand, within MPMX20_00541 at 565.039 kb on + strand, within MPMX20_00541 at 565.039 kb on + strand, within MPMX20_00541 at 565.047 kb on - strand, within MPMX20_00541 at 565.047 kb on - strand, within MPMX20_00541 at 565.047 kb on - strand, within MPMX20_00541 at 565.047 kb on - strand, within MPMX20_00541 at 565.047 kb on - strand, within MPMX20_00541 at 565.076 kb on + strand, within MPMX20_00541 at 565.156 kb on + strand, within MPMX20_00541 at 565.164 kb on - strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.228 kb on + strand, within MPMX20_00541 at 565.236 kb on - strand, within MPMX20_00541 at 565.236 kb on - strand, within MPMX20_00541 at 565.236 kb on - strand, within MPMX20_00541 at 565.246 kb on + strand, within MPMX20_00541 at 565.246 kb on - strand, within MPMX20_00541 at 565.344 kb on + strand, within MPMX20_00541 at 565.350 kb on - strand, within MPMX20_00541 at 565.515 kb on + strand at 565.557 kb on + strand at 565.578 kb on + strand at 565.578 kb on + strand at 565.592 kb on + strand at 565.600 kb on - strand at 565.600 kb on - strand at 565.723 kb on - strand at 565.799 kb on - strand, within MPMX20_00542 at 565.799 kb on - strand, within MPMX20_00542 at 565.818 kb on - strand, within MPMX20_00542 at 565.828 kb on - strand, within MPMX20_00542 at 565.830 kb on + strand, within MPMX20_00542 at 565.952 kb on + strand, within MPMX20_00542 at 565.952 kb on + strand, within MPMX20_00542 at 565.960 kb on - strand, within MPMX20_00542 at 565.960 kb on - strand, within MPMX20_00542 at 565.960 kb on - strand, within MPMX20_00542 at 565.961 kb on + strand, within MPMX20_00542 at 566.030 kb on - strand, within MPMX20_00542 at 566.066 kb on + strand, within MPMX20_00542 at 566.069 kb on - strand, within MPMX20_00542 at 566.071 kb on + strand, within MPMX20_00542 at 566.074 kb on - strand, within MPMX20_00542 at 566.074 kb on - strand, within MPMX20_00542 at 566.086 kb on + strand, within MPMX20_00542 at 566.094 kb on - strand, within MPMX20_00542 at 566.132 kb on + strand at 566.132 kb on + strand at 566.159 kb on + strand at 566.185 kb on - strand at 566.185 kb on - strand at 566.231 kb on - strand at 566.319 kb on + strand, within MPMX20_00543 at 566.328 kb on - strand, within MPMX20_00543 at 566.345 kb on + strand, within MPMX20_00543 at 566.358 kb on - strand, within MPMX20_00543 at 566.397 kb on - strand, within MPMX20_00543 at 566.397 kb on - strand, within MPMX20_00543 at 566.402 kb on + strand, within MPMX20_00543 at 566.411 kb on - strand, within MPMX20_00543 at 566.444 kb on + strand, within MPMX20_00543 at 566.510 kb on - strand, within MPMX20_00543 at 566.532 kb on + strand, within MPMX20_00543 at 566.546 kb on + strand, within MPMX20_00543 at 566.546 kb on - strand, within MPMX20_00543 at 566.556 kb on + strand, within MPMX20_00543 at 566.556 kb on + strand, within MPMX20_00543 at 566.561 kb on + strand, within MPMX20_00543 at 566.589 kb on + strand, within MPMX20_00543 at 566.594 kb on + strand, within MPMX20_00543 at 566.597 kb on - strand, within MPMX20_00543 at 566.597 kb on - strand, within MPMX20_00543 at 566.604 kb on + strand, within MPMX20_00543 at 566.612 kb on - strand, within MPMX20_00543 at 566.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, Mixed culture; Bacillus megaterium TBS_053 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 562,315 - MPMX20_00540 0.69 -1.3 562,344 - MPMX20_00540 0.70 -0.1 562,358 - MPMX20_00540 0.71 -0.5 562,389 + MPMX20_00540 0.72 -0.1 562,489 - MPMX20_00540 0.76 +0.9 562,631 + MPMX20_00540 0.81 -0.4 562,640 - MPMX20_00540 0.81 -2.1 562,728 + MPMX20_00540 0.84 -0.9 562,752 + MPMX20_00540 0.85 -1.1 562,760 - MPMX20_00540 0.86 -1.5 562,760 - MPMX20_00540 0.86 -1.3 562,765 - MPMX20_00540 0.86 -1.4 562,772 - MPMX20_00540 0.86 -1.9 562,793 + MPMX20_00540 0.87 +1.0 562,838 + MPMX20_00540 0.88 -0.1 562,863 - MPMX20_00540 0.89 +0.3 562,863 - MPMX20_00540 0.89 -0.2 562,870 + MPMX20_00540 0.90 +0.4 562,945 - -0.8 562,950 - +2.8 562,950 - +0.8 562,995 + -0.8 562,995 + -0.8 563,003 - +0.1 563,003 - +0.7 563,055 + +0.5 563,110 + +0.5 563,110 + +1.1 563,110 + -0.5 563,111 + -0.8 563,111 + +0.8 563,118 - -3.0 563,142 - -0.5 563,212 - -0.5 563,356 - -0.5 563,383 + -0.7 563,383 + -0.1 563,383 + +0.3 563,391 - -1.8 563,398 + -2.0 563,421 + -1.9 563,424 + -1.1 563,432 - -0.2 563,450 - +1.5 563,613 + MPMX20_00541 0.13 +0.3 563,704 + MPMX20_00541 0.17 -0.1 563,737 - MPMX20_00541 0.18 -0.1 563,824 + MPMX20_00541 0.22 +0.5 563,856 - MPMX20_00541 0.23 +1.5 563,928 + MPMX20_00541 0.26 +3.1 563,928 + MPMX20_00541 0.26 +0.5 563,936 - MPMX20_00541 0.27 -1.1 563,936 - MPMX20_00541 0.27 -1.4 563,962 + MPMX20_00541 0.28 +0.2 563,962 + MPMX20_00541 0.28 -0.3 563,962 + MPMX20_00541 0.28 +1.5 563,970 - MPMX20_00541 0.28 -2.0 563,970 - MPMX20_00541 0.28 +0.9 563,998 - MPMX20_00541 0.29 +2.2 564,000 + MPMX20_00541 0.29 -2.3 564,005 + MPMX20_00541 0.30 -1.7 564,008 - MPMX20_00541 0.30 -0.2 564,070 + MPMX20_00541 0.32 -1.7 564,083 - MPMX20_00541 0.33 -0.3 564,093 + MPMX20_00541 0.33 -0.1 564,093 + MPMX20_00541 0.33 -0.2 564,101 - MPMX20_00541 0.34 -1.0 564,101 - MPMX20_00541 0.34 -0.5 564,118 + MPMX20_00541 0.34 +0.0 564,118 + MPMX20_00541 0.34 +1.2 564,126 - MPMX20_00541 0.35 -1.7 564,286 + MPMX20_00541 0.41 +0.8 564,323 - MPMX20_00541 0.43 +0.0 564,331 - MPMX20_00541 0.43 -0.9 564,331 - MPMX20_00541 0.43 -2.2 564,370 + MPMX20_00541 0.45 +1.7 564,387 + MPMX20_00541 0.46 -2.2 564,436 + MPMX20_00541 0.48 +1.4 564,436 + MPMX20_00541 0.48 +0.7 564,443 - MPMX20_00541 0.48 -1.1 564,526 + MPMX20_00541 0.51 -0.5 564,534 - MPMX20_00541 0.52 +0.5 564,581 + MPMX20_00541 0.54 -0.7 564,613 - MPMX20_00541 0.55 -0.8 564,618 - MPMX20_00541 0.55 -1.7 564,660 + MPMX20_00541 0.57 +2.9 564,660 + MPMX20_00541 0.57 -1.8 564,668 - MPMX20_00541 0.57 -1.6 564,731 + MPMX20_00541 0.60 +0.2 564,739 - MPMX20_00541 0.60 -0.8 564,822 + MPMX20_00541 0.64 -0.1 564,822 + MPMX20_00541 0.64 -1.2 564,830 - MPMX20_00541 0.64 -1.8 564,830 - MPMX20_00541 0.64 +0.8 564,845 - MPMX20_00541 0.65 +0.7 564,895 + MPMX20_00541 0.67 -1.2 564,927 + MPMX20_00541 0.68 +1.2 564,935 - MPMX20_00541 0.69 -1.4 564,936 + MPMX20_00541 0.69 +0.3 565,020 + MPMX20_00541 0.72 -1.2 565,028 - MPMX20_00541 0.72 -1.3 565,039 + MPMX20_00541 0.73 -2.4 565,039 + MPMX20_00541 0.73 -0.1 565,047 - MPMX20_00541 0.73 -2.1 565,047 - MPMX20_00541 0.73 +0.7 565,047 - MPMX20_00541 0.73 -2.0 565,047 - MPMX20_00541 0.73 -1.5 565,047 - MPMX20_00541 0.73 -1.5 565,076 + MPMX20_00541 0.74 -1.1 565,156 + MPMX20_00541 0.78 +1.9 565,164 - MPMX20_00541 0.78 -0.2 565,228 + MPMX20_00541 0.81 -1.0 565,228 + MPMX20_00541 0.81 -1.6 565,228 + MPMX20_00541 0.81 -1.1 565,228 + MPMX20_00541 0.81 +1.4 565,228 + MPMX20_00541 0.81 -0.6 565,228 + MPMX20_00541 0.81 -1.7 565,228 + MPMX20_00541 0.81 -0.8 565,228 + MPMX20_00541 0.81 -2.5 565,228 + MPMX20_00541 0.81 -0.6 565,228 + MPMX20_00541 0.81 -0.6 565,228 + MPMX20_00541 0.81 +0.9 565,236 - MPMX20_00541 0.81 -2.1 565,236 - MPMX20_00541 0.81 +1.5 565,236 - MPMX20_00541 0.81 -1.1 565,246 + MPMX20_00541 0.82 -3.1 565,246 - MPMX20_00541 0.82 +0.5 565,344 + MPMX20_00541 0.86 -1.7 565,350 - MPMX20_00541 0.86 -1.3 565,515 + -0.8 565,557 + -0.1 565,578 + -0.4 565,578 + +1.5 565,592 + +0.1 565,600 - -1.5 565,600 - +1.3 565,723 - +5.3 565,799 - MPMX20_00542 0.12 +0.3 565,799 - MPMX20_00542 0.12 -0.7 565,818 - MPMX20_00542 0.17 -1.5 565,828 - MPMX20_00542 0.20 -1.5 565,830 + MPMX20_00542 0.20 +1.6 565,952 + MPMX20_00542 0.52 -0.3 565,952 + MPMX20_00542 0.52 -1.3 565,960 - MPMX20_00542 0.54 -0.6 565,960 - MPMX20_00542 0.54 -2.0 565,960 - MPMX20_00542 0.54 -1.1 565,961 + MPMX20_00542 0.54 -0.1 566,030 - MPMX20_00542 0.72 -2.3 566,066 + MPMX20_00542 0.81 +0.6 566,069 - MPMX20_00542 0.82 +0.4 566,071 + MPMX20_00542 0.83 +0.0 566,074 - MPMX20_00542 0.83 -1.8 566,074 - MPMX20_00542 0.83 -0.5 566,086 + MPMX20_00542 0.87 -1.1 566,094 - MPMX20_00542 0.89 +0.5 566,132 + +0.3 566,132 + -0.8 566,159 + +1.2 566,185 - -1.2 566,185 - -3.1 566,231 - -2.2 566,319 + MPMX20_00543 0.23 -0.5 566,328 - MPMX20_00543 0.25 -1.1 566,345 + MPMX20_00543 0.29 +0.5 566,358 - MPMX20_00543 0.32 -1.5 566,397 - MPMX20_00543 0.40 -0.2 566,397 - MPMX20_00543 0.40 -0.1 566,402 + MPMX20_00543 0.41 -1.2 566,411 - MPMX20_00543 0.43 +0.5 566,444 + MPMX20_00543 0.50 -1.0 566,510 - MPMX20_00543 0.65 +0.5 566,532 + MPMX20_00543 0.69 +2.3 566,546 + MPMX20_00543 0.72 -0.2 566,546 - MPMX20_00543 0.72 -1.4 566,556 + MPMX20_00543 0.74 +0.3 566,556 + MPMX20_00543 0.74 -1.2 566,561 + MPMX20_00543 0.76 -0.2 566,589 + MPMX20_00543 0.82 -0.4 566,594 + MPMX20_00543 0.83 -1.3 566,597 - MPMX20_00543 0.83 -1.8 566,597 - MPMX20_00543 0.83 +1.5 566,604 + MPMX20_00543 0.85 -1.1 566,612 - MPMX20_00543 0.87 +0.1 566,665 - +0.5
Or see this region's nucleotide sequence