Experiment: 0.1X LB plate, Mixed culture; Bacillus zanthoxyli TBS_056 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03320 and MPMX20_03321 overlap by 14 nucleotides MPMX20_03321 and MPMX20_03322 are separated by 212 nucleotides MPMX20_03322 and MPMX20_03323 overlap by 1 nucleotides MPMX20_03323 and MPMX20_03324 are separated by 18 nucleotides
MPMX20_03320: MPMX20_03320 - Inner membrane protein YfeZ, at 3,488,555 to 3,489,004
_03320
MPMX20_03321: MPMX20_03321 - Acetyltransferase YpeA, at 3,488,991 to 3,489,416
_03321
MPMX20_03322: MPMX20_03322 - N-acetylmuramoyl-L-alanine amidase AmiA, at 3,489,629 to 3,490,504
_03322
MPMX20_03323: MPMX20_03323 - Oxygen-dependent coproporphyrinogen-III oxidase, at 3,490,504 to 3,491,403
_03323
MPMX20_03324: MPMX20_03324 - NADP-dependent malic enzyme, at 3,491,422 to 3,493,701
_03324
Position (kb)
3489
3490
3491 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3488.662 kb on + strand, within MPMX20_03320 at 3488.678 kb on + strand, within MPMX20_03320 at 3488.678 kb on - strand, within MPMX20_03320 at 3488.739 kb on + strand, within MPMX20_03320 at 3488.846 kb on - strand, within MPMX20_03320 at 3488.846 kb on - strand, within MPMX20_03320 at 3488.867 kb on - strand, within MPMX20_03320 at 3488.906 kb on + strand, within MPMX20_03320 at 3488.926 kb on + strand, within MPMX20_03320 at 3488.995 kb on - strand at 3489.027 kb on + strand at 3489.035 kb on - strand, within MPMX20_03321 at 3489.051 kb on + strand, within MPMX20_03321 at 3489.059 kb on - strand, within MPMX20_03321 at 3489.255 kb on - strand, within MPMX20_03321 at 3489.362 kb on + strand, within MPMX20_03321 at 3489.362 kb on + strand, within MPMX20_03321 at 3489.362 kb on + strand, within MPMX20_03321 at 3489.362 kb on + strand, within MPMX20_03321 at 3489.370 kb on - strand, within MPMX20_03321 at 3489.370 kb on - strand, within MPMX20_03321 at 3489.378 kb on - strand at 3489.378 kb on - strand at 3489.575 kb on - strand at 3489.590 kb on + strand at 3489.644 kb on - strand at 3489.695 kb on + strand at 3489.732 kb on - strand, within MPMX20_03322 at 3489.802 kb on + strand, within MPMX20_03322 at 3489.802 kb on + strand, within MPMX20_03322 at 3489.810 kb on - strand, within MPMX20_03322 at 3489.816 kb on - strand, within MPMX20_03322 at 3489.892 kb on - strand, within MPMX20_03322 at 3489.971 kb on - strand, within MPMX20_03322 at 3489.996 kb on + strand, within MPMX20_03322 at 3490.008 kb on + strand, within MPMX20_03322 at 3490.075 kb on - strand, within MPMX20_03322 at 3490.084 kb on + strand, within MPMX20_03322 at 3490.092 kb on - strand, within MPMX20_03322 at 3490.213 kb on - strand, within MPMX20_03322 at 3490.236 kb on + strand, within MPMX20_03322 at 3490.275 kb on - strand, within MPMX20_03322 at 3490.332 kb on - strand, within MPMX20_03322 at 3490.405 kb on - strand, within MPMX20_03322 at 3490.445 kb on + strand at 3490.509 kb on + strand at 3490.580 kb on - strand at 3490.650 kb on + strand, within MPMX20_03323 at 3490.741 kb on + strand, within MPMX20_03323 at 3490.758 kb on - strand, within MPMX20_03323 at 3490.807 kb on - strand, within MPMX20_03323 at 3490.851 kb on + strand, within MPMX20_03323 at 3490.856 kb on + strand, within MPMX20_03323 at 3490.904 kb on + strand, within MPMX20_03323 at 3491.110 kb on - strand, within MPMX20_03323 at 3491.120 kb on + strand, within MPMX20_03323 at 3491.125 kb on + strand, within MPMX20_03323 at 3491.139 kb on - strand, within MPMX20_03323 at 3491.174 kb on + strand, within MPMX20_03323 at 3491.245 kb on - strand, within MPMX20_03323 at 3491.334 kb on - strand at 3491.344 kb on + strand at 3491.355 kb on + strand at 3491.394 kb on - strand at 3491.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Bacillus zanthoxyli TBS_056 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 3,488,662 + MPMX20_03320 0.24 +0.0 3,488,678 + MPMX20_03320 0.27 +1.2 3,488,678 - MPMX20_03320 0.27 +0.8 3,488,739 + MPMX20_03320 0.41 +1.4 3,488,846 - MPMX20_03320 0.65 +0.5 3,488,846 - MPMX20_03320 0.65 -0.3 3,488,867 - MPMX20_03320 0.69 -2.8 3,488,906 + MPMX20_03320 0.78 -0.8 3,488,926 + MPMX20_03320 0.82 -1.3 3,488,995 - +0.3 3,489,027 + +0.2 3,489,035 - MPMX20_03321 0.10 -1.2 3,489,051 + MPMX20_03321 0.14 -0.1 3,489,059 - MPMX20_03321 0.16 +1.2 3,489,255 - MPMX20_03321 0.62 +1.4 3,489,362 + MPMX20_03321 0.87 -0.3 3,489,362 + MPMX20_03321 0.87 +0.2 3,489,362 + MPMX20_03321 0.87 +2.4 3,489,362 + MPMX20_03321 0.87 +0.8 3,489,370 - MPMX20_03321 0.89 +1.0 3,489,370 - MPMX20_03321 0.89 -2.2 3,489,378 - -1.4 3,489,378 - -1.5 3,489,575 - +1.1 3,489,590 + -0.3 3,489,644 - +0.7 3,489,695 + +2.8 3,489,732 - MPMX20_03322 0.12 -0.8 3,489,802 + MPMX20_03322 0.20 +0.4 3,489,802 + MPMX20_03322 0.20 -0.1 3,489,810 - MPMX20_03322 0.21 +0.8 3,489,816 - MPMX20_03322 0.21 +3.1 3,489,892 - MPMX20_03322 0.30 -0.5 3,489,971 - MPMX20_03322 0.39 +0.3 3,489,996 + MPMX20_03322 0.42 +2.4 3,490,008 + MPMX20_03322 0.43 -0.1 3,490,075 - MPMX20_03322 0.51 +0.6 3,490,084 + MPMX20_03322 0.52 +0.4 3,490,092 - MPMX20_03322 0.53 +0.8 3,490,213 - MPMX20_03322 0.67 -1.4 3,490,236 + MPMX20_03322 0.69 +2.4 3,490,275 - MPMX20_03322 0.74 +1.1 3,490,332 - MPMX20_03322 0.80 -1.2 3,490,405 - MPMX20_03322 0.89 +0.7 3,490,445 + +0.0 3,490,509 + -1.2 3,490,580 - +0.1 3,490,650 + MPMX20_03323 0.16 +1.8 3,490,741 + MPMX20_03323 0.26 -0.9 3,490,758 - MPMX20_03323 0.28 +0.1 3,490,807 - MPMX20_03323 0.34 +1.2 3,490,851 + MPMX20_03323 0.39 +0.1 3,490,856 + MPMX20_03323 0.39 +0.1 3,490,904 + MPMX20_03323 0.44 +0.8 3,491,110 - MPMX20_03323 0.67 +0.1 3,491,120 + MPMX20_03323 0.68 -0.6 3,491,125 + MPMX20_03323 0.69 -0.3 3,491,139 - MPMX20_03323 0.71 -0.3 3,491,174 + MPMX20_03323 0.74 -0.2 3,491,245 - MPMX20_03323 0.82 -0.4 3,491,334 - +2.4 3,491,344 + +0.3 3,491,355 + -1.2 3,491,394 - -0.8 3,491,398 + +2.4
Or see this region's nucleotide sequence