Experiment: TSB plate, Mixed culture; Bacillus subtilis TBS_111 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04077 and MPMX20_04078 are separated by 165 nucleotides MPMX20_04078 and MPMX20_04079 are separated by 9 nucleotides MPMX20_04079 and MPMX20_04080 are separated by 223 nucleotides
MPMX20_04077: MPMX20_04077 - Argininosuccinate synthase, at 4,275,190 to 4,276,536
_04077
MPMX20_04078: MPMX20_04078 - tRNA-Leu, at 4,276,702 to 4,276,788
_04078
MPMX20_04079: MPMX20_04079 - Protein-export membrane protein SecG, at 4,276,798 to 4,277,130
_04079
MPMX20_04080: MPMX20_04080 - Phosphoglucosamine mutase, at 4,277,354 to 4,278,691
_04080
Position (kb)
4276
4277
4278 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4275.799 kb on + strand, within MPMX20_04077 at 4275.934 kb on - strand, within MPMX20_04077 at 4275.980 kb on - strand, within MPMX20_04077 at 4276.008 kb on + strand, within MPMX20_04077 at 4276.076 kb on + strand, within MPMX20_04077 at 4276.084 kb on + strand, within MPMX20_04077 at 4276.144 kb on - strand, within MPMX20_04077 at 4276.152 kb on + strand, within MPMX20_04077 at 4276.185 kb on - strand, within MPMX20_04077 at 4276.193 kb on - strand, within MPMX20_04077 at 4276.211 kb on + strand, within MPMX20_04077 at 4276.221 kb on + strand, within MPMX20_04077 at 4276.221 kb on + strand, within MPMX20_04077 at 4276.271 kb on + strand, within MPMX20_04077 at 4276.352 kb on - strand, within MPMX20_04077 at 4276.470 kb on + strand at 4276.511 kb on - strand at 4276.523 kb on + strand at 4276.795 kb on - strand at 4276.797 kb on - strand at 4277.027 kb on - strand, within MPMX20_04079 at 4277.074 kb on - strand, within MPMX20_04079 at 4277.267 kb on - strand at 4277.369 kb on - strand at 4277.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, Mixed culture; Bacillus subtilis TBS_111 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 4,275,799 + MPMX20_04077 0.45 -0.5 4,275,934 - MPMX20_04077 0.55 -0.1 4,275,980 - MPMX20_04077 0.59 +0.0 4,276,008 + MPMX20_04077 0.61 -0.2 4,276,076 + MPMX20_04077 0.66 -1.0 4,276,084 + MPMX20_04077 0.66 -0.7 4,276,144 - MPMX20_04077 0.71 -0.3 4,276,152 + MPMX20_04077 0.71 +2.9 4,276,185 - MPMX20_04077 0.74 +1.9 4,276,193 - MPMX20_04077 0.74 +0.0 4,276,211 + MPMX20_04077 0.76 -0.1 4,276,221 + MPMX20_04077 0.77 +0.5 4,276,221 + MPMX20_04077 0.77 +0.0 4,276,271 + MPMX20_04077 0.80 +4.0 4,276,352 - MPMX20_04077 0.86 +1.9 4,276,470 + +1.9 4,276,511 - +2.5 4,276,523 + -1.5 4,276,795 - -1.1 4,276,797 - +0.0 4,277,027 - MPMX20_04079 0.69 -2.6 4,277,074 - MPMX20_04079 0.83 -1.4 4,277,267 - +0.4 4,277,369 - -0.5 4,277,384 - +0.0
Or see this region's nucleotide sequence