Experiment: 1X LB plate, Mixed culture; Bacillus subtilis TBS_111 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01291 and MPMX20_01292 are separated by 139 nucleotides MPMX20_01292 and MPMX20_01293 are separated by 16 nucleotides
MPMX20_01291: MPMX20_01291 - D-alanyl-D-alanine carboxypeptidase DacA, at 1,351,335 to 1,352,546
_01291
MPMX20_01292: MPMX20_01292 - Endolytic peptidoglycan transglycosylase RlpA, at 1,352,686 to 1,353,798
_01292
MPMX20_01293: MPMX20_01293 - Peptidoglycan glycosyltransferase MrdB, at 1,353,815 to 1,354,927
_01293
Position (kb)
1352
1353
1354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1351.760 kb on - strand, within MPMX20_01291 at 1351.760 kb on - strand, within MPMX20_01291 at 1351.769 kb on - strand, within MPMX20_01291 at 1351.769 kb on - strand, within MPMX20_01291 at 1351.769 kb on - strand, within MPMX20_01291 at 1351.965 kb on - strand, within MPMX20_01291 at 1351.978 kb on + strand, within MPMX20_01291 at 1351.978 kb on + strand, within MPMX20_01291 at 1351.981 kb on - strand, within MPMX20_01291 at 1351.991 kb on - strand, within MPMX20_01291 at 1351.998 kb on + strand, within MPMX20_01291 at 1352.061 kb on + strand, within MPMX20_01291 at 1352.138 kb on + strand, within MPMX20_01291 at 1352.214 kb on - strand, within MPMX20_01291 at 1352.274 kb on - strand, within MPMX20_01291 at 1352.370 kb on + strand, within MPMX20_01291 at 1352.372 kb on - strand, within MPMX20_01291 at 1352.396 kb on + strand, within MPMX20_01291 at 1352.434 kb on - strand at 1352.439 kb on + strand at 1352.468 kb on - strand at 1352.473 kb on - strand at 1352.492 kb on + strand at 1352.494 kb on + strand at 1352.499 kb on + strand at 1352.504 kb on + strand at 1352.552 kb on + strand at 1352.739 kb on + strand at 1352.793 kb on + strand at 1352.826 kb on - strand, within MPMX20_01292 at 1352.871 kb on + strand, within MPMX20_01292 at 1352.879 kb on - strand, within MPMX20_01292 at 1352.885 kb on + strand, within MPMX20_01292 at 1352.949 kb on - strand, within MPMX20_01292 at 1353.136 kb on + strand, within MPMX20_01292 at 1353.144 kb on - strand, within MPMX20_01292 at 1353.193 kb on + strand, within MPMX20_01292 at 1353.216 kb on - strand, within MPMX20_01292 at 1353.216 kb on - strand, within MPMX20_01292 at 1353.251 kb on + strand, within MPMX20_01292 at 1353.302 kb on - strand, within MPMX20_01292 at 1353.306 kb on + strand, within MPMX20_01292 at 1353.319 kb on - strand, within MPMX20_01292 at 1353.334 kb on - strand, within MPMX20_01292 at 1353.342 kb on - strand, within MPMX20_01292 at 1353.352 kb on - strand, within MPMX20_01292 at 1353.376 kb on + strand, within MPMX20_01292 at 1353.438 kb on - strand, within MPMX20_01292 at 1353.510 kb on + strand, within MPMX20_01292 at 1353.555 kb on - strand, within MPMX20_01292 at 1353.561 kb on + strand, within MPMX20_01292 at 1353.569 kb on - strand, within MPMX20_01292 at 1353.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, Mixed culture; Bacillus subtilis TBS_111 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 1,351,760 - MPMX20_01291 0.35 -1.0 1,351,760 - MPMX20_01291 0.35 -2.8 1,351,769 - MPMX20_01291 0.36 -0.5 1,351,769 - MPMX20_01291 0.36 +0.4 1,351,769 - MPMX20_01291 0.36 +0.9 1,351,965 - MPMX20_01291 0.52 -0.6 1,351,978 + MPMX20_01291 0.53 -0.5 1,351,978 + MPMX20_01291 0.53 +0.0 1,351,981 - MPMX20_01291 0.53 -0.8 1,351,991 - MPMX20_01291 0.54 -1.2 1,351,998 + MPMX20_01291 0.55 -0.1 1,352,061 + MPMX20_01291 0.60 +0.0 1,352,138 + MPMX20_01291 0.66 -1.3 1,352,214 - MPMX20_01291 0.73 +0.1 1,352,274 - MPMX20_01291 0.77 -0.8 1,352,370 + MPMX20_01291 0.85 +0.4 1,352,372 - MPMX20_01291 0.86 -1.5 1,352,396 + MPMX20_01291 0.88 -2.3 1,352,434 - -1.4 1,352,439 + -1.1 1,352,468 - -1.2 1,352,473 - -0.1 1,352,492 + +1.0 1,352,494 + +0.2 1,352,499 + +0.4 1,352,504 + -1.1 1,352,552 + +1.1 1,352,739 + +1.2 1,352,793 + -1.7 1,352,826 - MPMX20_01292 0.13 -0.4 1,352,871 + MPMX20_01292 0.17 +2.7 1,352,879 - MPMX20_01292 0.17 +0.4 1,352,885 + MPMX20_01292 0.18 -0.8 1,352,949 - MPMX20_01292 0.24 -0.7 1,353,136 + MPMX20_01292 0.40 -0.8 1,353,144 - MPMX20_01292 0.41 -1.8 1,353,193 + MPMX20_01292 0.46 -1.0 1,353,216 - MPMX20_01292 0.48 -2.2 1,353,216 - MPMX20_01292 0.48 -1.6 1,353,251 + MPMX20_01292 0.51 -1.1 1,353,302 - MPMX20_01292 0.55 +0.7 1,353,306 + MPMX20_01292 0.56 +0.0 1,353,319 - MPMX20_01292 0.57 -1.0 1,353,334 - MPMX20_01292 0.58 +0.1 1,353,342 - MPMX20_01292 0.59 -1.1 1,353,352 - MPMX20_01292 0.60 +0.8 1,353,376 + MPMX20_01292 0.62 -3.0 1,353,438 - MPMX20_01292 0.68 -1.7 1,353,510 + MPMX20_01292 0.74 -1.1 1,353,555 - MPMX20_01292 0.78 +1.7 1,353,561 + MPMX20_01292 0.79 -1.8 1,353,569 - MPMX20_01292 0.79 -0.0 1,353,721 - -1.5
Or see this region's nucleotide sequence