Experiment: 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_104 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01100 and MPMX20_01101 are separated by 3 nucleotides MPMX20_01101 and MPMX20_01102 are separated by 174 nucleotides MPMX20_01102 and MPMX20_01103 are separated by 78 nucleotides MPMX20_01103 and MPMX20_01104 are separated by 72 nucleotides
MPMX20_01100: MPMX20_01100 - UDP-2,3-diacylglucosamine hydrolase, at 1,159,843 to 1,160,565
_01100
MPMX20_01101: MPMX20_01101 - Peptidyl-prolyl cis-trans isomerase B, at 1,160,569 to 1,161,063
_01101
MPMX20_01102: MPMX20_01102 - Cysteine--tRNA ligase, at 1,161,238 to 1,162,623
_01102
MPMX20_01103: MPMX20_01103 - Inner membrane protein YbcI, at 1,162,702 to 1,163,223
_01103
MPMX20_01104: MPMX20_01104 - Maltose regulon regulatory protein MalI, at 1,163,296 to 1,164,312
_01104
Position (kb) 1161
1162
1163 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 1160.686 kb on - strand, within MPMX20_01101 at 1160.686 kb on - strand, within MPMX20_01101 at 1160.716 kb on - strand, within MPMX20_01101 at 1160.716 kb on - strand, within MPMX20_01101 at 1161.086 kb on + strand at 1161.153 kb on + strand at 1161.153 kb on + strand at 1161.160 kb on + strand at 1162.629 kb on - strand at 1162.644 kb on - strand at 1162.698 kb on + strand at 1162.715 kb on + strand at 1162.723 kb on - strand at 1162.728 kb on - strand at 1162.742 kb on - strand at 1162.757 kb on - strand, within MPMX20_01103 at 1162.759 kb on + strand, within MPMX20_01103 at 1162.802 kb on - strand, within MPMX20_01103 at 1162.880 kb on - strand, within MPMX20_01103 at 1162.932 kb on + strand, within MPMX20_01103 at 1162.941 kb on + strand, within MPMX20_01103 at 1162.943 kb on + strand, within MPMX20_01103 at 1162.951 kb on - strand, within MPMX20_01103 at 1162.963 kb on + strand, within MPMX20_01103 at 1162.963 kb on + strand, within MPMX20_01103 at 1162.963 kb on + strand, within MPMX20_01103 at 1162.971 kb on - strand, within MPMX20_01103 at 1162.995 kb on + strand, within MPMX20_01103 at 1162.998 kb on - strand, within MPMX20_01103 at 1163.029 kb on + strand, within MPMX20_01103 at 1163.031 kb on - strand, within MPMX20_01103 at 1163.040 kb on + strand, within MPMX20_01103 at 1163.040 kb on + strand, within MPMX20_01103 at 1163.048 kb on - strand, within MPMX20_01103 at 1163.094 kb on - strand, within MPMX20_01103 at 1163.105 kb on + strand, within MPMX20_01103 at 1163.142 kb on - strand, within MPMX20_01103 at 1163.167 kb on + strand, within MPMX20_01103 at 1163.236 kb on + strand at 1163.289 kb on - strand at 1163.289 kb on - strand at 1163.320 kb on - strand at 1163.414 kb on - strand, within MPMX20_01104 at 1163.466 kb on + strand, within MPMX20_01104 at 1163.474 kb on - strand, within MPMX20_01104 at 1163.479 kb on - strand, within MPMX20_01104 at 1163.573 kb on + strand, within MPMX20_01104 at 1163.591 kb on - strand, within MPMX20_01104
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Bacillus pumilus TBS_104 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 1,160,686 - MPMX20_01101 0.24 -2.6 1,160,686 - MPMX20_01101 0.24 -1.4 1,160,716 - MPMX20_01101 0.30 -1.0 1,160,716 - MPMX20_01101 0.30 -0.7 1,161,086 + -2.6 1,161,153 + +0.3 1,161,153 + +1.4 1,161,160 + -1.3 1,162,629 - +0.7 1,162,644 - -2.7 1,162,698 + -0.1 1,162,715 + -1.0 1,162,723 - -0.7 1,162,728 - +1.3 1,162,742 - -0.6 1,162,757 - MPMX20_01103 0.11 -2.0 1,162,759 + MPMX20_01103 0.11 +1.5 1,162,802 - MPMX20_01103 0.19 -2.1 1,162,880 - MPMX20_01103 0.34 -0.9 1,162,932 + MPMX20_01103 0.44 +0.2 1,162,941 + MPMX20_01103 0.46 +2.2 1,162,943 + MPMX20_01103 0.46 +0.9 1,162,951 - MPMX20_01103 0.48 +1.3 1,162,963 + MPMX20_01103 0.50 -0.2 1,162,963 + MPMX20_01103 0.50 -1.2 1,162,963 + MPMX20_01103 0.50 +2.8 1,162,971 - MPMX20_01103 0.52 +0.7 1,162,995 + MPMX20_01103 0.56 +0.1 1,162,998 - MPMX20_01103 0.57 -1.3 1,163,029 + MPMX20_01103 0.63 +1.0 1,163,031 - MPMX20_01103 0.63 +0.9 1,163,040 + MPMX20_01103 0.65 -1.3 1,163,040 + MPMX20_01103 0.65 -0.3 1,163,048 - MPMX20_01103 0.66 +5.2 1,163,094 - MPMX20_01103 0.75 +1.3 1,163,105 + MPMX20_01103 0.77 +2.8 1,163,142 - MPMX20_01103 0.84 -0.2 1,163,167 + MPMX20_01103 0.89 -1.0 1,163,236 + -1.0 1,163,289 - -1.0 1,163,289 - -0.6 1,163,320 - +0.1 1,163,414 - MPMX20_01104 0.12 +0.3 1,163,466 + MPMX20_01104 0.17 +4.4 1,163,474 - MPMX20_01104 0.18 +0.4 1,163,479 - MPMX20_01104 0.18 +1.3 1,163,573 + MPMX20_01104 0.27 +3.3 1,163,591 - MPMX20_01104 0.29 -1.0
Or see this region's nucleotide sequence