Experiment: TSB plate, Mixed culture; Bacillus pumilus TBS_104 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00673 and MPMX20_00674 are separated by 303 nucleotides MPMX20_00674 and MPMX20_00675 are separated by 129 nucleotides MPMX20_00675 and MPMX20_00676 are separated by 120 nucleotides
MPMX20_00673: MPMX20_00673 - Peptide chain release factor RF3, at 691,257 to 692,846
_00673
MPMX20_00674: MPMX20_00674 - Osmotically-inducible protein Y, at 693,150 to 693,767
_00674
MPMX20_00675: MPMX20_00675 - hypothetical protein, at 693,897 to 694,058
_00675
MPMX20_00676: MPMX20_00676 - hypothetical protein, at 694,179 to 695,231
_00676
Position (kb) 693
694 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 692.168 kb on + strand, within MPMX20_00673 at 692.245 kb on - strand, within MPMX20_00673 at 692.296 kb on + strand, within MPMX20_00673 at 692.296 kb on + strand, within MPMX20_00673 at 692.296 kb on + strand, within MPMX20_00673 at 692.304 kb on - strand, within MPMX20_00673 at 692.304 kb on - strand, within MPMX20_00673 at 692.304 kb on - strand, within MPMX20_00673 at 692.304 kb on - strand, within MPMX20_00673 at 692.329 kb on - strand, within MPMX20_00673 at 692.368 kb on + strand, within MPMX20_00673 at 692.389 kb on + strand, within MPMX20_00673 at 692.397 kb on - strand, within MPMX20_00673 at 692.397 kb on - strand, within MPMX20_00673 at 692.399 kb on + strand, within MPMX20_00673 at 692.463 kb on - strand, within MPMX20_00673 at 692.529 kb on + strand, within MPMX20_00673 at 692.529 kb on + strand, within MPMX20_00673 at 692.537 kb on - strand, within MPMX20_00673 at 692.547 kb on + strand, within MPMX20_00673 at 692.583 kb on + strand, within MPMX20_00673 at 692.591 kb on - strand, within MPMX20_00673 at 692.612 kb on + strand, within MPMX20_00673 at 692.657 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.674 kb on + strand, within MPMX20_00673 at 692.682 kb on - strand, within MPMX20_00673 at 692.682 kb on - strand, within MPMX20_00673 at 692.682 kb on - strand, within MPMX20_00673 at 692.778 kb on - strand at 692.779 kb on + strand at 692.783 kb on - strand at 692.783 kb on - strand at 692.785 kb on + strand at 692.785 kb on + strand at 692.785 kb on + strand at 692.785 kb on + strand at 692.785 kb on + strand at 692.786 kb on + strand at 692.817 kb on + strand at 692.817 kb on + strand at 692.825 kb on - strand at 692.825 kb on - strand at 692.835 kb on + strand at 692.835 kb on + strand at 692.843 kb on + strand at 692.849 kb on - strand at 692.852 kb on + strand at 692.852 kb on + strand at 692.852 kb on + strand at 692.853 kb on + strand at 692.860 kb on - strand at 692.896 kb on + strand at 692.985 kb on - strand at 692.992 kb on + strand at 693.004 kb on + strand at 693.004 kb on + strand at 693.004 kb on + strand at 693.004 kb on + strand at 693.006 kb on + strand at 693.006 kb on + strand at 693.006 kb on + strand at 693.012 kb on - strand at 693.012 kb on - strand at 693.014 kb on - strand at 693.014 kb on - strand at 693.014 kb on - strand at 693.014 kb on - strand at 693.014 kb on - strand at 693.035 kb on + strand at 693.035 kb on - strand at 693.047 kb on + strand at 693.055 kb on - strand at 693.055 kb on - strand at 693.122 kb on + strand at 693.127 kb on + strand at 693.129 kb on + strand at 693.130 kb on - strand at 693.130 kb on - strand at 693.130 kb on - strand at 693.130 kb on - strand at 693.135 kb on - strand at 693.137 kb on - strand at 693.139 kb on + strand at 693.147 kb on - strand at 693.162 kb on + strand at 693.190 kb on + strand at 693.190 kb on + strand at 693.190 kb on + strand at 693.195 kb on + strand at 693.198 kb on - strand at 693.198 kb on - strand at 693.213 kb on - strand, within MPMX20_00674 at 693.217 kb on - strand, within MPMX20_00674 at 693.220 kb on + strand, within MPMX20_00674 at 693.220 kb on + strand, within MPMX20_00674 at 693.220 kb on + strand, within MPMX20_00674 at 693.220 kb on + strand, within MPMX20_00674 at 693.223 kb on - strand, within MPMX20_00674 at 693.228 kb on - strand, within MPMX20_00674 at 693.228 kb on - strand, within MPMX20_00674 at 693.228 kb on - strand, within MPMX20_00674 at 693.228 kb on - strand, within MPMX20_00674 at 693.236 kb on + strand, within MPMX20_00674 at 693.236 kb on + strand, within MPMX20_00674 at 693.241 kb on + strand, within MPMX20_00674 at 693.244 kb on - strand, within MPMX20_00674 at 693.260 kb on + strand, within MPMX20_00674 at 693.268 kb on - strand, within MPMX20_00674 at 693.355 kb on - strand, within MPMX20_00674 at 693.355 kb on - strand, within MPMX20_00674 at 693.375 kb on + strand, within MPMX20_00674 at 693.383 kb on - strand, within MPMX20_00674 at 693.383 kb on - strand, within MPMX20_00674 at 693.444 kb on + strand, within MPMX20_00674 at 693.449 kb on + strand, within MPMX20_00674 at 693.452 kb on - strand, within MPMX20_00674 at 693.453 kb on - strand, within MPMX20_00674 at 693.457 kb on - strand, within MPMX20_00674 at 693.484 kb on + strand, within MPMX20_00674 at 693.484 kb on + strand, within MPMX20_00674 at 693.492 kb on + strand, within MPMX20_00674 at 693.492 kb on + strand, within MPMX20_00674 at 693.501 kb on - strand, within MPMX20_00674 at 693.530 kb on - strand, within MPMX20_00674 at 693.546 kb on + strand, within MPMX20_00674 at 693.554 kb on - strand, within MPMX20_00674 at 693.630 kb on + strand, within MPMX20_00674 at 693.767 kb on + strand at 693.767 kb on + strand at 693.775 kb on + strand at 693.775 kb on + strand at 693.789 kb on + strand at 693.797 kb on - strand at 693.798 kb on - strand at 693.843 kb on + strand at 693.860 kb on - strand at 693.888 kb on - strand at 693.892 kb on + strand at 693.893 kb on + strand at 693.900 kb on - strand at 693.945 kb on + strand, within MPMX20_00675 at 693.945 kb on + strand, within MPMX20_00675 at 693.950 kb on + strand, within MPMX20_00675 at 693.965 kb on + strand, within MPMX20_00675 at 693.965 kb on + strand, within MPMX20_00675 at 693.973 kb on - strand, within MPMX20_00675 at 694.009 kb on + strand, within MPMX20_00675 at 694.009 kb on + strand, within MPMX20_00675 at 694.019 kb on + strand, within MPMX20_00675 at 694.032 kb on - strand, within MPMX20_00675 at 694.034 kb on + strand, within MPMX20_00675 at 694.034 kb on + strand, within MPMX20_00675 at 694.034 kb on + strand, within MPMX20_00675 at 694.042 kb on - strand at 694.042 kb on - strand at 694.042 kb on - strand at 694.042 kb on - strand at 694.042 kb on - strand at 694.127 kb on + strand at 694.127 kb on + strand at 694.205 kb on + strand at 694.205 kb on + strand at 694.210 kb on + strand at 694.213 kb on - strand at 694.213 kb on - strand at 694.233 kb on - strand at 694.259 kb on + strand at 694.259 kb on + strand at 694.267 kb on - strand at 694.270 kb on - strand at 694.376 kb on + strand, within MPMX20_00676 at 694.383 kb on + strand, within MPMX20_00676 at 694.383 kb on + strand, within MPMX20_00676 at 694.389 kb on - strand, within MPMX20_00676 at 694.391 kb on - strand, within MPMX20_00676 at 694.427 kb on + strand, within MPMX20_00676 at 694.499 kb on - strand, within MPMX20_00676 at 694.516 kb on + strand, within MPMX20_00676 at 694.541 kb on + strand, within MPMX20_00676 at 694.549 kb on - strand, within MPMX20_00676 at 694.605 kb on - strand, within MPMX20_00676 at 694.617 kb on + strand, within MPMX20_00676 at 694.701 kb on + strand, within MPMX20_00676
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, Mixed culture; Bacillus pumilus TBS_104 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 692,168 + MPMX20_00673 0.57 -1.5 692,245 - MPMX20_00673 0.62 -0.7 692,296 + MPMX20_00673 0.65 -2.2 692,296 + MPMX20_00673 0.65 -0.1 692,296 + MPMX20_00673 0.65 -0.7 692,304 - MPMX20_00673 0.66 -0.5 692,304 - MPMX20_00673 0.66 +1.7 692,304 - MPMX20_00673 0.66 -0.9 692,304 - MPMX20_00673 0.66 -0.1 692,329 - MPMX20_00673 0.67 -1.4 692,368 + MPMX20_00673 0.70 -1.5 692,389 + MPMX20_00673 0.71 -1.5 692,397 - MPMX20_00673 0.72 -1.9 692,397 - MPMX20_00673 0.72 -0.7 692,399 + MPMX20_00673 0.72 -4.2 692,463 - MPMX20_00673 0.76 -1.7 692,529 + MPMX20_00673 0.80 -2.1 692,529 + MPMX20_00673 0.80 -0.1 692,537 - MPMX20_00673 0.81 -0.9 692,547 + MPMX20_00673 0.81 -4.0 692,583 + MPMX20_00673 0.83 -2.0 692,591 - MPMX20_00673 0.84 +0.1 692,612 + MPMX20_00673 0.85 -0.5 692,657 + MPMX20_00673 0.88 +0.9 692,674 + MPMX20_00673 0.89 -3.8 692,674 + MPMX20_00673 0.89 -1.5 692,674 + MPMX20_00673 0.89 +0.1 692,674 + MPMX20_00673 0.89 -0.9 692,674 + MPMX20_00673 0.89 -3.0 692,674 + MPMX20_00673 0.89 -0.5 692,674 + MPMX20_00673 0.89 -1.0 692,674 + MPMX20_00673 0.89 +0.1 692,674 + MPMX20_00673 0.89 -3.1 692,674 + MPMX20_00673 0.89 -0.5 692,674 + MPMX20_00673 0.89 -1.7 692,682 - MPMX20_00673 0.90 -1.2 692,682 - MPMX20_00673 0.90 -3.6 692,682 - MPMX20_00673 0.90 -1.9 692,778 - -3.2 692,779 + -1.2 692,783 - -0.5 692,783 - -0.5 692,785 + -1.5 692,785 + -2.3 692,785 + -1.1 692,785 + -0.3 692,785 + -0.7 692,786 + -2.6 692,817 + -1.5 692,817 + -0.8 692,825 - -0.5 692,825 - -1.2 692,835 + -0.5 692,835 + +0.8 692,843 + -1.5 692,849 - -1.9 692,852 + -0.5 692,852 + +0.1 692,852 + -0.3 692,853 + -1.2 692,860 - -1.3 692,896 + +0.7 692,985 - -1.5 692,992 + -1.9 693,004 + +0.2 693,004 + -1.7 693,004 + +0.2 693,004 + -0.1 693,006 + +0.1 693,006 + -1.7 693,006 + -0.2 693,012 - +0.3 693,012 - -1.0 693,014 - -2.3 693,014 - -1.7 693,014 - +0.2 693,014 - -0.3 693,014 - -1.1 693,035 + -0.5 693,035 - -1.2 693,047 + -0.3 693,055 - +1.1 693,055 - -0.5 693,122 + -1.5 693,127 + +0.4 693,129 + +0.9 693,130 - +1.3 693,130 - -1.4 693,130 - -0.0 693,130 - +2.7 693,135 - +0.1 693,137 - -0.9 693,139 + +1.5 693,147 - +0.1 693,162 + -0.7 693,190 + +0.0 693,190 + -1.2 693,190 + -0.9 693,195 + -1.9 693,198 - -0.7 693,198 - -0.9 693,213 - MPMX20_00674 0.10 +1.5 693,217 - MPMX20_00674 0.11 -0.6 693,220 + MPMX20_00674 0.11 +0.3 693,220 + MPMX20_00674 0.11 -1.2 693,220 + MPMX20_00674 0.11 -1.8 693,220 + MPMX20_00674 0.11 -1.7 693,223 - MPMX20_00674 0.12 -0.8 693,228 - MPMX20_00674 0.13 +0.1 693,228 - MPMX20_00674 0.13 -0.7 693,228 - MPMX20_00674 0.13 -1.5 693,228 - MPMX20_00674 0.13 +0.9 693,236 + MPMX20_00674 0.14 -0.5 693,236 + MPMX20_00674 0.14 +2.1 693,241 + MPMX20_00674 0.15 +0.1 693,244 - MPMX20_00674 0.15 +0.4 693,260 + MPMX20_00674 0.18 -1.1 693,268 - MPMX20_00674 0.19 -0.4 693,355 - MPMX20_00674 0.33 +0.7 693,355 - MPMX20_00674 0.33 +0.1 693,375 + MPMX20_00674 0.36 +0.9 693,383 - MPMX20_00674 0.38 -0.5 693,383 - MPMX20_00674 0.38 +0.1 693,444 + MPMX20_00674 0.48 -0.5 693,449 + MPMX20_00674 0.48 -1.7 693,452 - MPMX20_00674 0.49 -0.4 693,453 - MPMX20_00674 0.49 -1.3 693,457 - MPMX20_00674 0.50 -0.2 693,484 + MPMX20_00674 0.54 -0.7 693,484 + MPMX20_00674 0.54 +0.1 693,492 + MPMX20_00674 0.55 -0.9 693,492 + MPMX20_00674 0.55 -0.7 693,501 - MPMX20_00674 0.57 -1.0 693,530 - MPMX20_00674 0.61 +1.0 693,546 + MPMX20_00674 0.64 -0.6 693,554 - MPMX20_00674 0.65 -1.5 693,630 + MPMX20_00674 0.78 -0.6 693,767 + -0.9 693,767 + -0.1 693,775 + -0.5 693,775 + +0.0 693,789 + -0.5 693,797 - -0.1 693,798 - -0.3 693,843 + -0.6 693,860 - -0.0 693,888 - +0.2 693,892 + -0.9 693,893 + -0.6 693,900 - -0.4 693,945 + MPMX20_00675 0.30 -0.9 693,945 + MPMX20_00675 0.30 -0.9 693,950 + MPMX20_00675 0.33 +2.1 693,965 + MPMX20_00675 0.42 +0.0 693,965 + MPMX20_00675 0.42 +2.5 693,973 - MPMX20_00675 0.47 -1.7 694,009 + MPMX20_00675 0.69 -0.8 694,009 + MPMX20_00675 0.69 +0.1 694,019 + MPMX20_00675 0.75 +0.3 694,032 - MPMX20_00675 0.83 -1.9 694,034 + MPMX20_00675 0.85 -0.7 694,034 + MPMX20_00675 0.85 -1.2 694,034 + MPMX20_00675 0.85 -0.1 694,042 - -0.0 694,042 - +0.1 694,042 - -1.2 694,042 - -0.5 694,042 - -0.9 694,127 + +1.9 694,127 + -0.2 694,205 + +0.5 694,205 + +0.7 694,210 + +0.6 694,213 - -0.1 694,213 - +2.3 694,233 - -0.6 694,259 + +1.1 694,259 + -0.6 694,267 - +1.1 694,270 - -0.7 694,376 + MPMX20_00676 0.19 -0.7 694,383 + MPMX20_00676 0.19 -2.2 694,383 + MPMX20_00676 0.19 +0.8 694,389 - MPMX20_00676 0.20 +0.0 694,391 - MPMX20_00676 0.20 -0.0 694,427 + MPMX20_00676 0.24 +0.4 694,499 - MPMX20_00676 0.30 -0.1 694,516 + MPMX20_00676 0.32 -0.7 694,541 + MPMX20_00676 0.34 -0.6 694,549 - MPMX20_00676 0.35 +1.3 694,605 - MPMX20_00676 0.40 -2.1 694,617 + MPMX20_00676 0.42 +0.8 694,701 + MPMX20_00676 0.50 -0.9
Or see this region's nucleotide sequence