Experiment: 1X LB plate, Mixed culture; Bacillus pumilus TBS_104 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00639 and MPMX20_00640 are separated by 9 nucleotides MPMX20_00640 and MPMX20_00641 are separated by 2 nucleotides MPMX20_00641 and MPMX20_00642 overlap by 4 nucleotides
MPMX20_00639: MPMX20_00639 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 664,689 to 665,069
_00639
MPMX20_00640: MPMX20_00640 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 665,079 to 665,936
_00640
MPMX20_00641: MPMX20_00641 - NAD/NADP-dependent betaine aldehyde dehydrogenase, at 665,939 to 667,405
_00641
MPMX20_00642: MPMX20_00642 - Homoprotocatechuate catabolism bifunctional isomerase/decarboxylase, at 667,402 to 668,679
_00642
Position (kb) 665
666
667
668 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 664.942 kb on + strand, within MPMX20_00639 at 664.944 kb on + strand, within MPMX20_00639 at 665.086 kb on + strand at 665.112 kb on + strand at 665.112 kb on + strand at 665.115 kb on - strand at 665.120 kb on - strand at 665.122 kb on + strand at 665.178 kb on - strand, within MPMX20_00640 at 665.233 kb on - strand, within MPMX20_00640 at 665.260 kb on + strand, within MPMX20_00640 at 665.268 kb on - strand, within MPMX20_00640 at 665.312 kb on - strand, within MPMX20_00640 at 665.338 kb on - strand, within MPMX20_00640 at 665.343 kb on - strand, within MPMX20_00640 at 665.453 kb on + strand, within MPMX20_00640 at 665.453 kb on + strand, within MPMX20_00640 at 665.467 kb on - strand, within MPMX20_00640 at 665.478 kb on + strand, within MPMX20_00640 at 665.478 kb on + strand, within MPMX20_00640 at 665.478 kb on + strand, within MPMX20_00640 at 665.486 kb on - strand, within MPMX20_00640 at 665.486 kb on - strand, within MPMX20_00640 at 665.486 kb on - strand, within MPMX20_00640 at 665.488 kb on + strand, within MPMX20_00640 at 665.488 kb on + strand, within MPMX20_00640 at 665.496 kb on - strand, within MPMX20_00640 at 665.496 kb on - strand, within MPMX20_00640 at 665.496 kb on - strand, within MPMX20_00640 at 665.512 kb on + strand, within MPMX20_00640 at 665.522 kb on + strand, within MPMX20_00640 at 665.522 kb on + strand, within MPMX20_00640 at 665.557 kb on - strand, within MPMX20_00640 at 665.568 kb on - strand, within MPMX20_00640 at 665.637 kb on - strand, within MPMX20_00640 at 665.715 kb on - strand, within MPMX20_00640 at 665.729 kb on - strand, within MPMX20_00640 at 665.752 kb on - strand, within MPMX20_00640 at 665.752 kb on - strand, within MPMX20_00640 at 665.752 kb on - strand, within MPMX20_00640 at 665.754 kb on + strand, within MPMX20_00640 at 665.788 kb on - strand, within MPMX20_00640 at 665.801 kb on + strand, within MPMX20_00640 at 665.809 kb on - strand, within MPMX20_00640 at 665.876 kb on + strand at 665.876 kb on + strand at 665.891 kb on - strand at 665.928 kb on - strand at 666.057 kb on - strand at 666.057 kb on - strand at 666.093 kb on - strand, within MPMX20_00641 at 666.142 kb on - strand, within MPMX20_00641 at 666.187 kb on - strand, within MPMX20_00641 at 666.187 kb on - strand, within MPMX20_00641 at 666.188 kb on - strand, within MPMX20_00641 at 666.200 kb on - strand, within MPMX20_00641 at 666.230 kb on - strand, within MPMX20_00641 at 666.250 kb on - strand, within MPMX20_00641 at 666.296 kb on + strand, within MPMX20_00641 at 666.358 kb on + strand, within MPMX20_00641 at 666.391 kb on + strand, within MPMX20_00641 at 666.421 kb on + strand, within MPMX20_00641 at 666.433 kb on + strand, within MPMX20_00641 at 666.472 kb on - strand, within MPMX20_00641 at 666.478 kb on + strand, within MPMX20_00641 at 666.562 kb on + strand, within MPMX20_00641 at 666.570 kb on + strand, within MPMX20_00641 at 666.578 kb on - strand, within MPMX20_00641 at 666.605 kb on - strand, within MPMX20_00641 at 666.605 kb on - strand, within MPMX20_00641 at 666.627 kb on - strand, within MPMX20_00641 at 666.673 kb on + strand, within MPMX20_00641 at 666.800 kb on + strand, within MPMX20_00641 at 666.800 kb on + strand, within MPMX20_00641 at 666.800 kb on + strand, within MPMX20_00641 at 666.800 kb on + strand, within MPMX20_00641 at 666.800 kb on + strand, within MPMX20_00641 at 666.808 kb on - strand, within MPMX20_00641 at 666.808 kb on - strand, within MPMX20_00641 at 666.808 kb on - strand, within MPMX20_00641 at 666.872 kb on - strand, within MPMX20_00641 at 666.883 kb on + strand, within MPMX20_00641 at 666.891 kb on - strand, within MPMX20_00641 at 666.891 kb on - strand, within MPMX20_00641 at 666.893 kb on + strand, within MPMX20_00641 at 666.924 kb on - strand, within MPMX20_00641 at 666.934 kb on + strand, within MPMX20_00641 at 666.956 kb on - strand, within MPMX20_00641 at 666.996 kb on - strand, within MPMX20_00641 at 667.006 kb on + strand, within MPMX20_00641 at 667.008 kb on + strand, within MPMX20_00641 at 667.009 kb on - strand, within MPMX20_00641 at 667.047 kb on + strand, within MPMX20_00641 at 667.157 kb on - strand, within MPMX20_00641 at 667.205 kb on + strand, within MPMX20_00641 at 667.321 kb on + strand at 667.336 kb on + strand at 667.336 kb on + strand at 667.344 kb on - strand at 667.499 kb on + strand at 667.593 kb on - strand, within MPMX20_00642 at 667.641 kb on + strand, within MPMX20_00642 at 667.641 kb on + strand, within MPMX20_00642 at 667.649 kb on - strand, within MPMX20_00642 at 667.655 kb on - strand, within MPMX20_00642 at 667.656 kb on + strand, within MPMX20_00642 at 667.728 kb on + strand, within MPMX20_00642 at 667.796 kb on + strand, within MPMX20_00642 at 667.804 kb on - strand, within MPMX20_00642 at 667.809 kb on - strand, within MPMX20_00642 at 667.817 kb on + strand, within MPMX20_00642 at 667.818 kb on + strand, within MPMX20_00642 at 667.883 kb on + strand, within MPMX20_00642 at 667.886 kb on - strand, within MPMX20_00642 at 667.886 kb on - strand, within MPMX20_00642 at 667.927 kb on + strand, within MPMX20_00642 at 667.927 kb on + strand, within MPMX20_00642 at 667.992 kb on - strand, within MPMX20_00642 at 668.007 kb on - strand, within MPMX20_00642 at 668.009 kb on + strand, within MPMX20_00642 at 668.017 kb on - strand, within MPMX20_00642 at 668.017 kb on - strand, within MPMX20_00642 at 668.031 kb on + strand, within MPMX20_00642 at 668.031 kb on + strand, within MPMX20_00642 at 668.031 kb on + strand, within MPMX20_00642 at 668.039 kb on - strand, within MPMX20_00642 at 668.039 kb on - strand, within MPMX20_00642 at 668.039 kb on - strand, within MPMX20_00642 at 668.123 kb on + strand, within MPMX20_00642 at 668.145 kb on + strand, within MPMX20_00642 at 668.153 kb on - strand, within MPMX20_00642 at 668.153 kb on - strand, within MPMX20_00642 at 668.217 kb on - strand, within MPMX20_00642 at 668.235 kb on + strand, within MPMX20_00642 at 668.243 kb on - strand, within MPMX20_00642 at 668.243 kb on - strand, within MPMX20_00642 at 668.251 kb on - strand, within MPMX20_00642 at 668.300 kb on - strand, within MPMX20_00642 at 668.331 kb on + strand, within MPMX20_00642 at 668.331 kb on + strand, within MPMX20_00642
Per-strain Table
Position Strand Gene LocusTag Fraction 1X LB plate, Mixed culture; Bacillus pumilus TBS_104 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 664,942 + MPMX20_00639 0.66 -0.4 664,944 + MPMX20_00639 0.67 +1.3 665,086 + -0.7 665,112 + -0.8 665,112 + -0.9 665,115 - +0.2 665,120 - +0.5 665,122 + -0.4 665,178 - MPMX20_00640 0.12 +0.1 665,233 - MPMX20_00640 0.18 -0.2 665,260 + MPMX20_00640 0.21 +1.2 665,268 - MPMX20_00640 0.22 -0.3 665,312 - MPMX20_00640 0.27 -0.6 665,338 - MPMX20_00640 0.30 -2.7 665,343 - MPMX20_00640 0.31 -0.4 665,453 + MPMX20_00640 0.44 -0.8 665,453 + MPMX20_00640 0.44 -1.4 665,467 - MPMX20_00640 0.45 -0.7 665,478 + MPMX20_00640 0.47 -0.8 665,478 + MPMX20_00640 0.47 -2.0 665,478 + MPMX20_00640 0.47 -0.5 665,486 - MPMX20_00640 0.47 +1.8 665,486 - MPMX20_00640 0.47 -0.4 665,486 - MPMX20_00640 0.47 +0.4 665,488 + MPMX20_00640 0.48 -0.8 665,488 + MPMX20_00640 0.48 -0.6 665,496 - MPMX20_00640 0.49 +0.7 665,496 - MPMX20_00640 0.49 -0.7 665,496 - MPMX20_00640 0.49 -1.8 665,512 + MPMX20_00640 0.50 -0.4 665,522 + MPMX20_00640 0.52 -0.8 665,522 + MPMX20_00640 0.52 +1.7 665,557 - MPMX20_00640 0.56 -1.2 665,568 - MPMX20_00640 0.57 -1.6 665,637 - MPMX20_00640 0.65 +0.4 665,715 - MPMX20_00640 0.74 -1.5 665,729 - MPMX20_00640 0.76 -0.9 665,752 - MPMX20_00640 0.78 -0.6 665,752 - MPMX20_00640 0.78 -0.6 665,752 - MPMX20_00640 0.78 -2.2 665,754 + MPMX20_00640 0.79 -1.2 665,788 - MPMX20_00640 0.83 -0.3 665,801 + MPMX20_00640 0.84 -0.5 665,809 - MPMX20_00640 0.85 -1.5 665,876 + -1.4 665,876 + +0.2 665,891 - +0.2 665,928 - +0.3 666,057 - -0.4 666,057 - +1.8 666,093 - MPMX20_00641 0.10 -2.5 666,142 - MPMX20_00641 0.14 -1.3 666,187 - MPMX20_00641 0.17 +0.4 666,187 - MPMX20_00641 0.17 -1.6 666,188 - MPMX20_00641 0.17 -2.1 666,200 - MPMX20_00641 0.18 -2.3 666,230 - MPMX20_00641 0.20 -3.5 666,250 - MPMX20_00641 0.21 -3.4 666,296 + MPMX20_00641 0.24 -1.6 666,358 + MPMX20_00641 0.29 -0.2 666,391 + MPMX20_00641 0.31 -0.9 666,421 + MPMX20_00641 0.33 -0.7 666,433 + MPMX20_00641 0.34 -2.8 666,472 - MPMX20_00641 0.36 -0.1 666,478 + MPMX20_00641 0.37 -1.5 666,562 + MPMX20_00641 0.42 -0.6 666,570 + MPMX20_00641 0.43 -1.2 666,578 - MPMX20_00641 0.44 -0.2 666,605 - MPMX20_00641 0.45 -1.8 666,605 - MPMX20_00641 0.45 -5.0 666,627 - MPMX20_00641 0.47 -0.9 666,673 + MPMX20_00641 0.50 +0.5 666,800 + MPMX20_00641 0.59 -1.8 666,800 + MPMX20_00641 0.59 -0.4 666,800 + MPMX20_00641 0.59 -1.6 666,800 + MPMX20_00641 0.59 -1.3 666,800 + MPMX20_00641 0.59 -1.1 666,808 - MPMX20_00641 0.59 -0.4 666,808 - MPMX20_00641 0.59 -1.6 666,808 - MPMX20_00641 0.59 -1.2 666,872 - MPMX20_00641 0.64 -1.5 666,883 + MPMX20_00641 0.64 -0.7 666,891 - MPMX20_00641 0.65 -4.0 666,891 - MPMX20_00641 0.65 -2.9 666,893 + MPMX20_00641 0.65 +2.2 666,924 - MPMX20_00641 0.67 -2.0 666,934 + MPMX20_00641 0.68 -0.8 666,956 - MPMX20_00641 0.69 -1.6 666,996 - MPMX20_00641 0.72 -1.6 667,006 + MPMX20_00641 0.73 +0.2 667,008 + MPMX20_00641 0.73 -2.0 667,009 - MPMX20_00641 0.73 -1.6 667,047 + MPMX20_00641 0.76 -3.1 667,157 - MPMX20_00641 0.83 +0.2 667,205 + MPMX20_00641 0.86 +0.0 667,321 + -0.7 667,336 + -0.6 667,336 + -1.6 667,344 - -0.6 667,499 + -0.2 667,593 - MPMX20_00642 0.15 -2.2 667,641 + MPMX20_00642 0.19 -1.6 667,641 + MPMX20_00642 0.19 -2.9 667,649 - MPMX20_00642 0.19 -1.4 667,655 - MPMX20_00642 0.20 +0.6 667,656 + MPMX20_00642 0.20 +1.3 667,728 + MPMX20_00642 0.26 +1.2 667,796 + MPMX20_00642 0.31 -0.5 667,804 - MPMX20_00642 0.31 -1.6 667,809 - MPMX20_00642 0.32 -0.1 667,817 + MPMX20_00642 0.32 +0.0 667,818 + MPMX20_00642 0.33 +0.8 667,883 + MPMX20_00642 0.38 -1.4 667,886 - MPMX20_00642 0.38 -1.1 667,886 - MPMX20_00642 0.38 +0.2 667,927 + MPMX20_00642 0.41 -1.7 667,927 + MPMX20_00642 0.41 +1.3 667,992 - MPMX20_00642 0.46 +0.8 668,007 - MPMX20_00642 0.47 -0.8 668,009 + MPMX20_00642 0.47 -0.5 668,017 - MPMX20_00642 0.48 -0.5 668,017 - MPMX20_00642 0.48 +0.3 668,031 + MPMX20_00642 0.49 +0.7 668,031 + MPMX20_00642 0.49 +1.2 668,031 + MPMX20_00642 0.49 +1.7 668,039 - MPMX20_00642 0.50 -0.7 668,039 - MPMX20_00642 0.50 -2.4 668,039 - MPMX20_00642 0.50 -0.9 668,123 + MPMX20_00642 0.56 -0.4 668,145 + MPMX20_00642 0.58 -0.6 668,153 - MPMX20_00642 0.59 -0.8 668,153 - MPMX20_00642 0.59 +1.2 668,217 - MPMX20_00642 0.64 -1.4 668,235 + MPMX20_00642 0.65 +0.4 668,243 - MPMX20_00642 0.66 -1.2 668,243 - MPMX20_00642 0.66 +0.3 668,251 - MPMX20_00642 0.66 -0.7 668,300 - MPMX20_00642 0.70 +0.8 668,331 + MPMX20_00642 0.73 -0.6 668,331 + MPMX20_00642 0.73 -0.8
Or see this region's nucleotide sequence