Experiment: M9 plate, Mixed culture; Bacillus pumilus TBS_103 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01049 and MPMX20_01050 are separated by 87 nucleotides MPMX20_01050 and MPMX20_01051 are separated by 78 nucleotides
MPMX20_01049: MPMX20_01049 - HTH-type transcriptional regulator NorG, at 1,103,646 to 1,105,067
_01049
MPMX20_01050: MPMX20_01050 - hypothetical protein, at 1,105,155 to 1,105,766
_01050
MPMX20_01051: MPMX20_01051 - Lactose operon repressor, at 1,105,845 to 1,106,915
_01051
Position (kb)
1105
1106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1104.158 kb on - strand, within MPMX20_01049 at 1104.359 kb on - strand, within MPMX20_01049 at 1104.369 kb on - strand, within MPMX20_01049 at 1104.384 kb on + strand, within MPMX20_01049 at 1104.384 kb on + strand, within MPMX20_01049 at 1104.440 kb on - strand, within MPMX20_01049 at 1104.535 kb on - strand, within MPMX20_01049 at 1104.557 kb on + strand, within MPMX20_01049 at 1104.595 kb on + strand, within MPMX20_01049 at 1104.595 kb on + strand, within MPMX20_01049 at 1104.690 kb on + strand, within MPMX20_01049 at 1104.698 kb on - strand, within MPMX20_01049 at 1104.698 kb on - strand, within MPMX20_01049 at 1104.698 kb on - strand, within MPMX20_01049 at 1104.698 kb on - strand, within MPMX20_01049 at 1104.699 kb on - strand, within MPMX20_01049 at 1104.713 kb on + strand, within MPMX20_01049 at 1104.802 kb on - strand, within MPMX20_01049 at 1104.859 kb on + strand, within MPMX20_01049 at 1104.872 kb on - strand, within MPMX20_01049 at 1104.879 kb on + strand, within MPMX20_01049 at 1104.888 kb on - strand, within MPMX20_01049 at 1104.910 kb on - strand, within MPMX20_01049 at 1104.920 kb on + strand, within MPMX20_01049 at 1104.967 kb on - strand at 1105.000 kb on + strand at 1105.013 kb on - strand at 1105.176 kb on - strand at 1105.194 kb on - strand at 1105.223 kb on - strand, within MPMX20_01050 at 1105.263 kb on - strand, within MPMX20_01050 at 1105.263 kb on - strand, within MPMX20_01050 at 1105.263 kb on - strand, within MPMX20_01050 at 1105.427 kb on - strand, within MPMX20_01050 at 1105.434 kb on - strand, within MPMX20_01050 at 1105.482 kb on - strand, within MPMX20_01050 at 1105.505 kb on + strand, within MPMX20_01050 at 1105.519 kb on + strand, within MPMX20_01050 at 1105.553 kb on - strand, within MPMX20_01050 at 1105.608 kb on - strand, within MPMX20_01050 at 1105.627 kb on + strand, within MPMX20_01050 at 1105.715 kb on - strand at 1105.717 kb on + strand at 1105.875 kb on - strand at 1105.877 kb on + strand at 1105.934 kb on - strand at 1106.001 kb on + strand, within MPMX20_01051 at 1106.106 kb on + strand, within MPMX20_01051 at 1106.106 kb on + strand, within MPMX20_01051 at 1106.114 kb on - strand, within MPMX20_01051 at 1106.151 kb on + strand, within MPMX20_01051 at 1106.154 kb on - strand, within MPMX20_01051 at 1106.156 kb on + strand, within MPMX20_01051 at 1106.252 kb on - strand, within MPMX20_01051 at 1106.294 kb on - strand, within MPMX20_01051 at 1106.311 kb on + strand, within MPMX20_01051 at 1106.318 kb on + strand, within MPMX20_01051 at 1106.328 kb on + strand, within MPMX20_01051 at 1106.402 kb on + strand, within MPMX20_01051 at 1106.436 kb on + strand, within MPMX20_01051 at 1106.444 kb on - strand, within MPMX20_01051 at 1106.444 kb on - strand, within MPMX20_01051 at 1106.459 kb on + strand, within MPMX20_01051 at 1106.467 kb on - strand, within MPMX20_01051 at 1106.492 kb on + strand, within MPMX20_01051 at 1106.584 kb on - strand, within MPMX20_01051 at 1106.606 kb on - strand, within MPMX20_01051 at 1106.713 kb on - strand, within MPMX20_01051 at 1106.759 kb on + strand, within MPMX20_01051
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, Mixed culture; Bacillus pumilus TBS_103 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 1,104,158 - MPMX20_01049 0.36 +0.6 1,104,359 - MPMX20_01049 0.50 -0.5 1,104,369 - MPMX20_01049 0.51 -1.3 1,104,384 + MPMX20_01049 0.52 -0.6 1,104,384 + MPMX20_01049 0.52 +0.2 1,104,440 - MPMX20_01049 0.56 +0.5 1,104,535 - MPMX20_01049 0.63 +1.3 1,104,557 + MPMX20_01049 0.64 +2.7 1,104,595 + MPMX20_01049 0.67 +1.0 1,104,595 + MPMX20_01049 0.67 +1.3 1,104,690 + MPMX20_01049 0.73 +1.5 1,104,698 - MPMX20_01049 0.74 +1.4 1,104,698 - MPMX20_01049 0.74 -0.3 1,104,698 - MPMX20_01049 0.74 -0.4 1,104,698 - MPMX20_01049 0.74 +0.4 1,104,699 - MPMX20_01049 0.74 -2.0 1,104,713 + MPMX20_01049 0.75 +2.5 1,104,802 - MPMX20_01049 0.81 +1.5 1,104,859 + MPMX20_01049 0.85 -1.5 1,104,872 - MPMX20_01049 0.86 -1.5 1,104,879 + MPMX20_01049 0.87 -0.6 1,104,888 - MPMX20_01049 0.87 -0.4 1,104,910 - MPMX20_01049 0.89 -0.9 1,104,920 + MPMX20_01049 0.90 +0.5 1,104,967 - -0.7 1,105,000 + -0.3 1,105,013 - -0.3 1,105,176 - -1.0 1,105,194 - +0.3 1,105,223 - MPMX20_01050 0.11 -0.4 1,105,263 - MPMX20_01050 0.18 +0.1 1,105,263 - MPMX20_01050 0.18 -1.9 1,105,263 - MPMX20_01050 0.18 -0.0 1,105,427 - MPMX20_01050 0.44 -1.1 1,105,434 - MPMX20_01050 0.46 -1.4 1,105,482 - MPMX20_01050 0.53 -1.0 1,105,505 + MPMX20_01050 0.57 +0.1 1,105,519 + MPMX20_01050 0.59 -1.3 1,105,553 - MPMX20_01050 0.65 -0.4 1,105,608 - MPMX20_01050 0.74 -3.0 1,105,627 + MPMX20_01050 0.77 -2.2 1,105,715 - +0.3 1,105,717 + -0.6 1,105,875 - -1.9 1,105,877 + -1.5 1,105,934 - -4.0 1,106,001 + MPMX20_01051 0.15 -0.5 1,106,106 + MPMX20_01051 0.24 +0.0 1,106,106 + MPMX20_01051 0.24 -0.1 1,106,114 - MPMX20_01051 0.25 -0.6 1,106,151 + MPMX20_01051 0.29 -0.8 1,106,154 - MPMX20_01051 0.29 +2.1 1,106,156 + MPMX20_01051 0.29 -0.6 1,106,252 - MPMX20_01051 0.38 +1.3 1,106,294 - MPMX20_01051 0.42 -1.9 1,106,311 + MPMX20_01051 0.44 -0.8 1,106,318 + MPMX20_01051 0.44 -1.4 1,106,328 + MPMX20_01051 0.45 -3.0 1,106,402 + MPMX20_01051 0.52 +2.3 1,106,436 + MPMX20_01051 0.55 +0.5 1,106,444 - MPMX20_01051 0.56 -1.0 1,106,444 - MPMX20_01051 0.56 -1.5 1,106,459 + MPMX20_01051 0.57 -1.3 1,106,467 - MPMX20_01051 0.58 +0.5 1,106,492 + MPMX20_01051 0.60 -0.4 1,106,584 - MPMX20_01051 0.69 +1.0 1,106,606 - MPMX20_01051 0.71 -0.0 1,106,713 - MPMX20_01051 0.81 -0.7 1,106,759 + MPMX20_01051 0.85 -3.3
Or see this region's nucleotide sequence