Experiment: 0.1X LB plate, Mixed culture; Pseudomonas lini TBS_028 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00215 and MPMX20_00216 overlap by 8 nucleotides MPMX20_00216 and MPMX20_00217 overlap by 1 nucleotides MPMX20_00217 and MPMX20_00218 are separated by 286 nucleotides MPMX20_00218 and MPMX20_00219 are separated by 95 nucleotides
MPMX20_00215: MPMX20_00215 - Sulfur carrier protein ThiS adenylyltransferase, at 224,288 to 225,043
_00215
MPMX20_00216: MPMX20_00216 - Thiamine-phosphate synthase, at 225,036 to 225,671
_00216
MPMX20_00217: MPMX20_00217 - Phosphomethylpyrimidine synthase, at 225,671 to 227,566
_00217
MPMX20_00218: MPMX20_00218 - Regulator of sigma D, at 227,853 to 228,350
_00218
MPMX20_00219: MPMX20_00219 - NADH pyrophosphatase, at 228,446 to 229,219
_00219
Position (kb) 225
226
227
228 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.690 kb on - strand, within MPMX20_00215 at 224.691 kb on - strand, within MPMX20_00215 at 224.695 kb on - strand, within MPMX20_00215 at 224.718 kb on + strand, within MPMX20_00215 at 224.875 kb on + strand, within MPMX20_00215 at 224.883 kb on - strand, within MPMX20_00215 at 224.885 kb on + strand, within MPMX20_00215 at 224.890 kb on + strand, within MPMX20_00215 at 224.890 kb on + strand, within MPMX20_00215 at 224.898 kb on - strand, within MPMX20_00215 at 224.901 kb on + strand, within MPMX20_00215 at 225.082 kb on + strand at 225.122 kb on - strand, within MPMX20_00216 at 225.159 kb on - strand, within MPMX20_00216 at 225.200 kb on + strand, within MPMX20_00216 at 225.200 kb on + strand, within MPMX20_00216 at 225.253 kb on - strand, within MPMX20_00216 at 225.274 kb on + strand, within MPMX20_00216 at 225.317 kb on + strand, within MPMX20_00216 at 225.353 kb on - strand, within MPMX20_00216 at 225.382 kb on + strand, within MPMX20_00216 at 225.389 kb on + strand, within MPMX20_00216 at 225.408 kb on + strand, within MPMX20_00216 at 225.427 kb on + strand, within MPMX20_00216 at 225.427 kb on + strand, within MPMX20_00216 at 225.427 kb on - strand, within MPMX20_00216 at 225.435 kb on - strand, within MPMX20_00216 at 225.481 kb on + strand, within MPMX20_00216 at 225.481 kb on + strand, within MPMX20_00216 at 225.489 kb on - strand, within MPMX20_00216 at 225.522 kb on + strand, within MPMX20_00216 at 225.523 kb on + strand, within MPMX20_00216 at 225.531 kb on - strand, within MPMX20_00216 at 225.568 kb on - strand, within MPMX20_00216 at 225.570 kb on + strand, within MPMX20_00216 at 225.570 kb on + strand, within MPMX20_00216 at 225.580 kb on + strand, within MPMX20_00216 at 225.588 kb on - strand, within MPMX20_00216 at 225.602 kb on + strand, within MPMX20_00216 at 225.610 kb on - strand at 225.612 kb on + strand at 225.612 kb on + strand at 225.620 kb on - strand at 225.669 kb on - strand at 225.745 kb on + strand at 225.745 kb on + strand at 225.753 kb on - strand at 225.753 kb on - strand at 225.753 kb on - strand at 225.770 kb on + strand at 225.770 kb on + strand at 225.770 kb on + strand at 225.778 kb on + strand at 225.778 kb on + strand at 225.778 kb on - strand at 225.778 kb on - strand at 225.786 kb on - strand at 225.786 kb on - strand at 225.786 kb on - strand at 225.786 kb on - strand at 225.878 kb on - strand, within MPMX20_00217 at 225.878 kb on - strand, within MPMX20_00217 at 226.033 kb on + strand, within MPMX20_00217 at 226.036 kb on - strand, within MPMX20_00217 at 226.077 kb on - strand, within MPMX20_00217 at 226.086 kb on - strand, within MPMX20_00217 at 226.088 kb on + strand, within MPMX20_00217 at 226.093 kb on - strand, within MPMX20_00217 at 226.136 kb on + strand, within MPMX20_00217 at 226.142 kb on - strand, within MPMX20_00217 at 226.153 kb on - strand, within MPMX20_00217 at 226.153 kb on - strand, within MPMX20_00217 at 226.153 kb on - strand, within MPMX20_00217 at 226.177 kb on + strand, within MPMX20_00217 at 226.185 kb on - strand, within MPMX20_00217 at 226.191 kb on - strand, within MPMX20_00217 at 226.210 kb on + strand, within MPMX20_00217 at 226.267 kb on - strand, within MPMX20_00217 at 226.302 kb on + strand, within MPMX20_00217 at 226.368 kb on + strand, within MPMX20_00217 at 226.368 kb on + strand, within MPMX20_00217 at 226.442 kb on + strand, within MPMX20_00217 at 226.501 kb on - strand, within MPMX20_00217 at 226.513 kb on - strand, within MPMX20_00217 at 226.575 kb on + strand, within MPMX20_00217 at 226.583 kb on + strand, within MPMX20_00217 at 226.583 kb on + strand, within MPMX20_00217 at 226.590 kb on - strand, within MPMX20_00217 at 226.591 kb on - strand, within MPMX20_00217 at 226.591 kb on - strand, within MPMX20_00217 at 226.592 kb on - strand, within MPMX20_00217 at 226.635 kb on - strand, within MPMX20_00217 at 226.684 kb on + strand, within MPMX20_00217 at 226.717 kb on - strand, within MPMX20_00217 at 226.726 kb on + strand, within MPMX20_00217 at 226.786 kb on + strand, within MPMX20_00217 at 226.786 kb on + strand, within MPMX20_00217 at 226.786 kb on + strand, within MPMX20_00217 at 226.791 kb on + strand, within MPMX20_00217 at 226.794 kb on - strand, within MPMX20_00217 at 226.794 kb on - strand, within MPMX20_00217 at 226.860 kb on + strand, within MPMX20_00217 at 226.903 kb on - strand, within MPMX20_00217 at 226.993 kb on + strand, within MPMX20_00217 at 227.021 kb on + strand, within MPMX20_00217 at 227.051 kb on + strand, within MPMX20_00217 at 227.130 kb on - strand, within MPMX20_00217 at 227.130 kb on - strand, within MPMX20_00217 at 227.190 kb on - strand, within MPMX20_00217 at 227.213 kb on + strand, within MPMX20_00217 at 227.322 kb on - strand, within MPMX20_00217 at 227.332 kb on + strand, within MPMX20_00217 at 227.340 kb on - strand, within MPMX20_00217 at 227.340 kb on - strand, within MPMX20_00217 at 227.342 kb on + strand, within MPMX20_00217 at 227.350 kb on - strand, within MPMX20_00217 at 227.416 kb on + strand at 227.497 kb on + strand at 227.505 kb on - strand at 227.505 kb on - strand at 227.536 kb on + strand at 227.544 kb on - strand at 227.561 kb on + strand at 227.571 kb on - strand at 227.589 kb on - strand at 227.647 kb on - strand at 227.678 kb on + strand at 227.681 kb on - strand at 227.681 kb on - strand at 227.683 kb on + strand at 227.712 kb on + strand at 227.712 kb on + strand at 227.725 kb on + strand at 227.811 kb on - strand at 227.814 kb on + strand at 227.814 kb on - strand at 227.862 kb on - strand at 227.928 kb on - strand, within MPMX20_00218 at 227.970 kb on - strand, within MPMX20_00218 at 227.970 kb on - strand, within MPMX20_00218 at 227.989 kb on + strand, within MPMX20_00218 at 227.992 kb on - strand, within MPMX20_00218 at 227.997 kb on + strand, within MPMX20_00218 at 228.058 kb on + strand, within MPMX20_00218 at 228.070 kb on + strand, within MPMX20_00218 at 228.098 kb on + strand, within MPMX20_00218 at 228.098 kb on + strand, within MPMX20_00218 at 228.167 kb on - strand, within MPMX20_00218 at 228.174 kb on - strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.230 kb on + strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.238 kb on - strand, within MPMX20_00218 at 228.262 kb on + strand, within MPMX20_00218 at 228.269 kb on - strand, within MPMX20_00218 at 228.270 kb on - strand, within MPMX20_00218 at 228.270 kb on - strand, within MPMX20_00218 at 228.273 kb on + strand, within MPMX20_00218 at 228.278 kb on + strand, within MPMX20_00218 at 228.278 kb on + strand, within MPMX20_00218 at 228.278 kb on + strand, within MPMX20_00218 at 228.286 kb on - strand, within MPMX20_00218 at 228.286 kb on - strand, within MPMX20_00218 at 228.286 kb on - strand, within MPMX20_00218 at 228.286 kb on - strand, within MPMX20_00218 at 228.286 kb on - strand, within MPMX20_00218 at 228.310 kb on + strand at 228.341 kb on + strand at 228.341 kb on + strand at 228.349 kb on + strand at 228.366 kb on + strand at 228.366 kb on + strand at 228.366 kb on + strand at 228.366 kb on + strand at 228.366 kb on + strand at 228.374 kb on - strand at 228.374 kb on - strand at 228.394 kb on - strand at 228.406 kb on + strand at 228.406 kb on + strand at 228.417 kb on - strand at 228.425 kb on + strand at 228.433 kb on - strand at 228.433 kb on - strand at 228.433 kb on - strand at 228.435 kb on + strand at 228.470 kb on + strand at 228.531 kb on + strand, within MPMX20_00219 at 228.532 kb on + strand, within MPMX20_00219 at 228.538 kb on - strand, within MPMX20_00219 at 228.538 kb on - strand, within MPMX20_00219 at 228.538 kb on - strand, within MPMX20_00219
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Pseudomonas lini TBS_028 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 224,672 + MPMX20_00215 0.51 -0.3 224,672 + MPMX20_00215 0.51 +3.1 224,672 + MPMX20_00215 0.51 -2.0 224,672 + MPMX20_00215 0.51 +1.0 224,672 + MPMX20_00215 0.51 -2.8 224,672 + MPMX20_00215 0.51 -1.7 224,672 + MPMX20_00215 0.51 -1.4 224,672 + MPMX20_00215 0.51 -2.9 224,680 - MPMX20_00215 0.52 -0.4 224,680 - MPMX20_00215 0.52 -0.5 224,680 - MPMX20_00215 0.52 -0.5 224,680 - MPMX20_00215 0.52 +0.7 224,680 - MPMX20_00215 0.52 +0.7 224,690 - MPMX20_00215 0.53 -3.3 224,691 - MPMX20_00215 0.53 -2.6 224,695 - MPMX20_00215 0.54 +0.7 224,718 + MPMX20_00215 0.57 -1.1 224,875 + MPMX20_00215 0.78 -2.1 224,883 - MPMX20_00215 0.79 -0.8 224,885 + MPMX20_00215 0.79 +0.0 224,890 + MPMX20_00215 0.80 -0.6 224,890 + MPMX20_00215 0.80 -0.3 224,898 - MPMX20_00215 0.81 -1.4 224,901 + MPMX20_00215 0.81 -0.2 225,082 + +0.1 225,122 - MPMX20_00216 0.14 -0.6 225,159 - MPMX20_00216 0.19 -0.7 225,200 + MPMX20_00216 0.26 +1.2 225,200 + MPMX20_00216 0.26 +0.8 225,253 - MPMX20_00216 0.34 -1.3 225,274 + MPMX20_00216 0.37 -2.9 225,317 + MPMX20_00216 0.44 -0.5 225,353 - MPMX20_00216 0.50 -3.7 225,382 + MPMX20_00216 0.54 -0.7 225,389 + MPMX20_00216 0.56 +0.5 225,408 + MPMX20_00216 0.58 -0.3 225,427 + MPMX20_00216 0.61 -0.1 225,427 + MPMX20_00216 0.61 -1.8 225,427 - MPMX20_00216 0.61 +0.2 225,435 - MPMX20_00216 0.63 +0.2 225,481 + MPMX20_00216 0.70 -2.2 225,481 + MPMX20_00216 0.70 -2.4 225,489 - MPMX20_00216 0.71 -0.1 225,522 + MPMX20_00216 0.76 +0.2 225,523 + MPMX20_00216 0.77 -2.2 225,531 - MPMX20_00216 0.78 +1.0 225,568 - MPMX20_00216 0.84 -0.9 225,570 + MPMX20_00216 0.84 +0.5 225,570 + MPMX20_00216 0.84 -0.2 225,580 + MPMX20_00216 0.86 -0.8 225,588 - MPMX20_00216 0.87 -1.4 225,602 + MPMX20_00216 0.89 +3.0 225,610 - -1.2 225,612 + -1.2 225,612 + -0.1 225,620 - -3.4 225,669 - -3.4 225,745 + -0.9 225,745 + -0.6 225,753 - -1.5 225,753 - -0.8 225,753 - +0.3 225,770 + -0.6 225,770 + -0.4 225,770 + -1.3 225,778 + -2.2 225,778 + -3.8 225,778 - +0.9 225,778 - +0.4 225,786 - +0.8 225,786 - +0.5 225,786 - -1.2 225,786 - +0.7 225,878 - MPMX20_00217 0.11 -2.6 225,878 - MPMX20_00217 0.11 +1.2 226,033 + MPMX20_00217 0.19 -0.9 226,036 - MPMX20_00217 0.19 +0.8 226,077 - MPMX20_00217 0.21 -0.6 226,086 - MPMX20_00217 0.22 +0.2 226,088 + MPMX20_00217 0.22 +0.8 226,093 - MPMX20_00217 0.22 -1.2 226,136 + MPMX20_00217 0.25 -0.2 226,142 - MPMX20_00217 0.25 +3.1 226,153 - MPMX20_00217 0.25 +1.0 226,153 - MPMX20_00217 0.25 -1.1 226,153 - MPMX20_00217 0.25 -1.9 226,177 + MPMX20_00217 0.27 +0.8 226,185 - MPMX20_00217 0.27 +2.1 226,191 - MPMX20_00217 0.27 -1.4 226,210 + MPMX20_00217 0.28 +2.8 226,267 - MPMX20_00217 0.31 -1.2 226,302 + MPMX20_00217 0.33 -0.2 226,368 + MPMX20_00217 0.37 +0.2 226,368 + MPMX20_00217 0.37 -4.0 226,442 + MPMX20_00217 0.41 +0.7 226,501 - MPMX20_00217 0.44 +1.1 226,513 - MPMX20_00217 0.44 -1.8 226,575 + MPMX20_00217 0.48 -1.1 226,583 + MPMX20_00217 0.48 +2.2 226,583 + MPMX20_00217 0.48 +1.0 226,590 - MPMX20_00217 0.48 +0.2 226,591 - MPMX20_00217 0.49 +0.0 226,591 - MPMX20_00217 0.49 +2.3 226,592 - MPMX20_00217 0.49 -0.8 226,635 - MPMX20_00217 0.51 -3.2 226,684 + MPMX20_00217 0.53 +0.4 226,717 - MPMX20_00217 0.55 -0.1 226,726 + MPMX20_00217 0.56 -0.8 226,786 + MPMX20_00217 0.59 +1.3 226,786 + MPMX20_00217 0.59 -0.4 226,786 + MPMX20_00217 0.59 +1.1 226,791 + MPMX20_00217 0.59 -0.8 226,794 - MPMX20_00217 0.59 +0.4 226,794 - MPMX20_00217 0.59 +2.3 226,860 + MPMX20_00217 0.63 -1.7 226,903 - MPMX20_00217 0.65 +1.0 226,993 + MPMX20_00217 0.70 +0.3 227,021 + MPMX20_00217 0.71 +0.2 227,051 + MPMX20_00217 0.73 -1.2 227,130 - MPMX20_00217 0.77 +0.5 227,130 - MPMX20_00217 0.77 -0.5 227,190 - MPMX20_00217 0.80 -0.6 227,213 + MPMX20_00217 0.81 +1.8 227,322 - MPMX20_00217 0.87 +0.2 227,332 + MPMX20_00217 0.88 +3.2 227,340 - MPMX20_00217 0.88 -2.6 227,340 - MPMX20_00217 0.88 +1.2 227,342 + MPMX20_00217 0.88 -0.2 227,350 - MPMX20_00217 0.89 +1.8 227,416 + -2.0 227,497 + +0.8 227,505 - -2.2 227,505 - -0.6 227,536 + -0.4 227,544 - +0.8 227,561 + -1.2 227,571 - -1.9 227,589 - -3.3 227,647 - -2.9 227,678 + +0.2 227,681 - -2.0 227,681 - -0.6 227,683 + -0.2 227,712 + -1.0 227,712 + -2.6 227,725 + -0.5 227,811 - -0.7 227,814 + -0.2 227,814 - -0.8 227,862 - +0.2 227,928 - MPMX20_00218 0.15 -0.5 227,970 - MPMX20_00218 0.23 -0.8 227,970 - MPMX20_00218 0.23 +0.6 227,989 + MPMX20_00218 0.27 +0.2 227,992 - MPMX20_00218 0.28 -2.4 227,997 + MPMX20_00218 0.29 +3.3 228,058 + MPMX20_00218 0.41 -0.8 228,070 + MPMX20_00218 0.44 +1.8 228,098 + MPMX20_00218 0.49 -2.3 228,098 + MPMX20_00218 0.49 -1.2 228,167 - MPMX20_00218 0.63 +1.3 228,174 - MPMX20_00218 0.64 -0.5 228,230 + MPMX20_00218 0.76 +0.1 228,230 + MPMX20_00218 0.76 +1.3 228,230 + MPMX20_00218 0.76 -1.4 228,230 + MPMX20_00218 0.76 +0.8 228,230 + MPMX20_00218 0.76 -1.6 228,230 + MPMX20_00218 0.76 +0.7 228,230 + MPMX20_00218 0.76 +2.6 228,238 - MPMX20_00218 0.77 +0.2 228,238 - MPMX20_00218 0.77 +1.6 228,238 - MPMX20_00218 0.77 -2.0 228,238 - MPMX20_00218 0.77 -0.1 228,238 - MPMX20_00218 0.77 +1.3 228,238 - MPMX20_00218 0.77 +1.2 228,238 - MPMX20_00218 0.77 +0.8 228,238 - MPMX20_00218 0.77 -0.2 228,238 - MPMX20_00218 0.77 -0.1 228,238 - MPMX20_00218 0.77 +1.1 228,238 - MPMX20_00218 0.77 -0.2 228,238 - MPMX20_00218 0.77 +0.2 228,238 - MPMX20_00218 0.77 -1.3 228,238 - MPMX20_00218 0.77 +0.8 228,238 - MPMX20_00218 0.77 -1.7 228,238 - MPMX20_00218 0.77 -1.0 228,238 - MPMX20_00218 0.77 +1.0 228,238 - MPMX20_00218 0.77 +0.2 228,238 - MPMX20_00218 0.77 -0.4 228,262 + MPMX20_00218 0.82 +1.4 228,269 - MPMX20_00218 0.84 -0.6 228,270 - MPMX20_00218 0.84 +0.1 228,270 - MPMX20_00218 0.84 -1.6 228,273 + MPMX20_00218 0.84 -0.8 228,278 + MPMX20_00218 0.85 +2.5 228,278 + MPMX20_00218 0.85 -0.2 228,278 + MPMX20_00218 0.85 -0.2 228,286 - MPMX20_00218 0.87 +0.2 228,286 - MPMX20_00218 0.87 +1.9 228,286 - MPMX20_00218 0.87 +0.9 228,286 - MPMX20_00218 0.87 +0.7 228,286 - MPMX20_00218 0.87 -0.6 228,310 + -0.6 228,341 + -0.1 228,341 + -0.5 228,349 + +0.8 228,366 + +0.2 228,366 + +2.2 228,366 + -2.9 228,366 + -0.8 228,366 + -1.1 228,374 - +0.0 228,374 - +2.0 228,394 - -1.2 228,406 + -0.0 228,406 + +1.6 228,417 - -0.5 228,425 + -0.4 228,433 - +0.6 228,433 - +0.8 228,433 - -0.1 228,435 + +0.2 228,470 + -1.1 228,531 + MPMX20_00219 0.11 -0.5 228,532 + MPMX20_00219 0.11 +3.0 228,538 - MPMX20_00219 0.12 +0.8 228,538 - MPMX20_00219 0.12 +1.0 228,538 - MPMX20_00219 0.12 -1.7
Or see this region's nucleotide sequence