Experiment: 0.1X LB plate, Mixed culture; Enterobacter sp. TBS-079 (Tn7_Kan_Lux) 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00303 and MPMX20_00304 overlap by 89 nucleotides MPMX20_00304 and MPMX20_00305 overlap by 17 nucleotides MPMX20_00305 and MPMX20_00306 are separated by 10 nucleotides
MPMX20_00303: MPMX20_00303 - Sensor histidine kinase RcsC, at 323,992 to 326,313
_00303
MPMX20_00304: MPMX20_00304 - D-allose kinase, at 326,225 to 327,169
_00304
MPMX20_00305: MPMX20_00305 - D-allulose-6-phosphate 3-epimerase, at 327,153 to 327,848
_00305
MPMX20_00306: MPMX20_00306 - D-allose transport system permease protein AlsC, at 327,859 to 328,839
_00306
Position (kb) 326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 325.270 kb on - strand, within MPMX20_00303 at 325.270 kb on - strand, within MPMX20_00303 at 325.270 kb on - strand, within MPMX20_00303 at 325.284 kb on - strand, within MPMX20_00303 at 325.379 kb on + strand, within MPMX20_00303 at 325.396 kb on + strand, within MPMX20_00303 at 325.422 kb on + strand, within MPMX20_00303 at 325.465 kb on - strand, within MPMX20_00303 at 325.598 kb on + strand, within MPMX20_00303 at 325.598 kb on + strand, within MPMX20_00303 at 325.606 kb on - strand, within MPMX20_00303 at 325.606 kb on - strand, within MPMX20_00303 at 325.638 kb on + strand, within MPMX20_00303 at 325.646 kb on - strand, within MPMX20_00303 at 325.712 kb on - strand, within MPMX20_00303 at 325.748 kb on + strand, within MPMX20_00303 at 325.748 kb on + strand, within MPMX20_00303 at 325.755 kb on - strand, within MPMX20_00303 at 325.756 kb on - strand, within MPMX20_00303 at 325.756 kb on - strand, within MPMX20_00303 at 325.756 kb on - strand, within MPMX20_00303 at 325.765 kb on + strand, within MPMX20_00303 at 325.821 kb on - strand, within MPMX20_00303 at 325.822 kb on - strand, within MPMX20_00303 at 325.861 kb on - strand, within MPMX20_00303 at 325.879 kb on + strand, within MPMX20_00303 at 325.879 kb on + strand, within MPMX20_00303 at 325.963 kb on - strand, within MPMX20_00303 at 326.020 kb on - strand, within MPMX20_00303 at 326.072 kb on + strand, within MPMX20_00303 at 326.080 kb on - strand, within MPMX20_00303 at 326.080 kb on - strand, within MPMX20_00303 at 326.080 kb on - strand, within MPMX20_00303 at 326.091 kb on + strand at 326.091 kb on + strand at 326.099 kb on + strand at 326.107 kb on - strand at 326.114 kb on + strand at 326.115 kb on - strand at 326.117 kb on - strand at 326.134 kb on - strand at 326.134 kb on - strand at 326.214 kb on + strand at 326.214 kb on - strand at 326.221 kb on + strand at 326.229 kb on - strand at 326.229 kb on - strand at 326.231 kb on + strand at 326.299 kb on + strand at 326.305 kb on - strand at 326.305 kb on - strand at 326.307 kb on + strand at 326.315 kb on - strand at 326.346 kb on + strand, within MPMX20_00304 at 326.357 kb on - strand, within MPMX20_00304 at 326.364 kb on - strand, within MPMX20_00304 at 326.375 kb on - strand, within MPMX20_00304 at 326.464 kb on + strand, within MPMX20_00304 at 326.474 kb on + strand, within MPMX20_00304 at 326.482 kb on - strand, within MPMX20_00304 at 326.483 kb on + strand, within MPMX20_00304 at 326.519 kb on + strand, within MPMX20_00304 at 326.529 kb on - strand, within MPMX20_00304 at 326.545 kb on - strand, within MPMX20_00304 at 326.601 kb on - strand, within MPMX20_00304 at 326.628 kb on + strand, within MPMX20_00304 at 326.655 kb on + strand, within MPMX20_00304 at 326.661 kb on - strand, within MPMX20_00304 at 326.696 kb on + strand, within MPMX20_00304 at 326.706 kb on + strand, within MPMX20_00304 at 326.713 kb on - strand, within MPMX20_00304 at 326.714 kb on - strand, within MPMX20_00304 at 326.714 kb on - strand, within MPMX20_00304 at 326.738 kb on + strand, within MPMX20_00304 at 326.808 kb on - strand, within MPMX20_00304 at 326.808 kb on - strand, within MPMX20_00304 at 326.928 kb on - strand, within MPMX20_00304 at 326.928 kb on - strand, within MPMX20_00304 at 326.950 kb on + strand, within MPMX20_00304 at 326.950 kb on + strand, within MPMX20_00304 at 327.009 kb on - strand, within MPMX20_00304 at 327.009 kb on - strand, within MPMX20_00304 at 327.032 kb on + strand, within MPMX20_00304 at 327.032 kb on + strand, within MPMX20_00304 at 327.040 kb on - strand, within MPMX20_00304 at 327.075 kb on - strand at 327.077 kb on + strand at 327.085 kb on - strand at 327.085 kb on - strand at 327.089 kb on + strand at 327.096 kb on + strand at 327.096 kb on + strand at 327.104 kb on - strand at 327.114 kb on - strand at 327.116 kb on + strand at 327.137 kb on - strand at 327.141 kb on - strand at 327.178 kb on + strand at 327.249 kb on + strand, within MPMX20_00305 at 327.252 kb on - strand, within MPMX20_00305 at 327.254 kb on + strand, within MPMX20_00305 at 327.254 kb on + strand, within MPMX20_00305 at 327.254 kb on + strand, within MPMX20_00305 at 327.257 kb on - strand, within MPMX20_00305 at 327.262 kb on - strand, within MPMX20_00305 at 327.262 kb on - strand, within MPMX20_00305 at 327.351 kb on - strand, within MPMX20_00305 at 327.353 kb on + strand, within MPMX20_00305 at 327.354 kb on + strand, within MPMX20_00305 at 327.354 kb on + strand, within MPMX20_00305 at 327.373 kb on + strand, within MPMX20_00305 at 327.427 kb on - strand, within MPMX20_00305 at 327.436 kb on - strand, within MPMX20_00305 at 327.507 kb on + strand, within MPMX20_00305 at 327.607 kb on + strand, within MPMX20_00305 at 327.615 kb on - strand, within MPMX20_00305 at 327.615 kb on - strand, within MPMX20_00305 at 327.631 kb on + strand, within MPMX20_00305 at 327.634 kb on - strand, within MPMX20_00305 at 327.639 kb on - strand, within MPMX20_00305 at 327.666 kb on + strand, within MPMX20_00305 at 327.674 kb on + strand, within MPMX20_00305 at 327.674 kb on - strand, within MPMX20_00305 at 327.744 kb on - strand, within MPMX20_00305 at 327.762 kb on + strand, within MPMX20_00305 at 327.779 kb on + strand at 327.787 kb on - strand at 327.815 kb on + strand at 327.815 kb on + strand at 327.815 kb on + strand at 327.815 kb on + strand at 327.823 kb on - strand at 327.823 kb on - strand at 327.823 kb on - strand at 327.823 kb on - strand at 327.825 kb on + strand at 327.825 kb on - strand at 327.939 kb on - strand at 328.089 kb on + strand, within MPMX20_00306 at 328.097 kb on + strand, within MPMX20_00306 at 328.097 kb on + strand, within MPMX20_00306 at 328.111 kb on + strand, within MPMX20_00306 at 328.117 kb on + strand, within MPMX20_00306 at 328.126 kb on + strand, within MPMX20_00306 at 328.131 kb on + strand, within MPMX20_00306 at 328.131 kb on + strand, within MPMX20_00306 at 328.139 kb on - strand, within MPMX20_00306 at 328.153 kb on + strand, within MPMX20_00306
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Enterobacter sp. TBS-079 (Tn7_Kan_Lux) 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 325,270 - MPMX20_00303 0.55 +1.0 325,270 - MPMX20_00303 0.55 +0.3 325,270 - MPMX20_00303 0.55 +3.1 325,284 - MPMX20_00303 0.56 +1.2 325,379 + MPMX20_00303 0.60 -1.2 325,396 + MPMX20_00303 0.60 -0.6 325,422 + MPMX20_00303 0.62 -1.2 325,465 - MPMX20_00303 0.63 -0.6 325,598 + MPMX20_00303 0.69 +0.8 325,598 + MPMX20_00303 0.69 +0.7 325,606 - MPMX20_00303 0.70 +1.7 325,606 - MPMX20_00303 0.70 -0.2 325,638 + MPMX20_00303 0.71 +0.2 325,646 - MPMX20_00303 0.71 +0.5 325,712 - MPMX20_00303 0.74 -1.4 325,748 + MPMX20_00303 0.76 +0.7 325,748 + MPMX20_00303 0.76 +1.2 325,755 - MPMX20_00303 0.76 -1.6 325,756 - MPMX20_00303 0.76 +0.5 325,756 - MPMX20_00303 0.76 -2.2 325,756 - MPMX20_00303 0.76 +0.7 325,765 + MPMX20_00303 0.76 +0.9 325,821 - MPMX20_00303 0.79 +0.5 325,822 - MPMX20_00303 0.79 +1.1 325,861 - MPMX20_00303 0.80 +1.9 325,879 + MPMX20_00303 0.81 -1.6 325,879 + MPMX20_00303 0.81 -0.7 325,963 - MPMX20_00303 0.85 +1.8 326,020 - MPMX20_00303 0.87 -0.8 326,072 + MPMX20_00303 0.90 -0.5 326,080 - MPMX20_00303 0.90 +0.2 326,080 - MPMX20_00303 0.90 -0.6 326,080 - MPMX20_00303 0.90 +0.3 326,091 + -0.4 326,091 + +0.3 326,099 + -0.7 326,107 - -1.2 326,114 + +0.0 326,115 - -0.7 326,117 - -1.6 326,134 - +0.1 326,134 - -1.2 326,214 + +1.9 326,214 - +0.8 326,221 + +0.4 326,229 - +1.5 326,229 - -0.1 326,231 + -0.2 326,299 + -2.3 326,305 - -2.3 326,305 - -1.0 326,307 + +1.6 326,315 - +1.8 326,346 + MPMX20_00304 0.13 -0.5 326,357 - MPMX20_00304 0.14 +0.3 326,364 - MPMX20_00304 0.15 -0.9 326,375 - MPMX20_00304 0.16 +1.3 326,464 + MPMX20_00304 0.25 -0.8 326,474 + MPMX20_00304 0.26 +1.3 326,482 - MPMX20_00304 0.27 -0.6 326,483 + MPMX20_00304 0.27 +1.7 326,519 + MPMX20_00304 0.31 +1.2 326,529 - MPMX20_00304 0.32 -0.7 326,545 - MPMX20_00304 0.34 +1.2 326,601 - MPMX20_00304 0.40 -0.4 326,628 + MPMX20_00304 0.43 -0.6 326,655 + MPMX20_00304 0.46 +1.2 326,661 - MPMX20_00304 0.46 -1.6 326,696 + MPMX20_00304 0.50 +1.2 326,706 + MPMX20_00304 0.51 -1.3 326,713 - MPMX20_00304 0.52 -2.6 326,714 - MPMX20_00304 0.52 -0.8 326,714 - MPMX20_00304 0.52 -1.3 326,738 + MPMX20_00304 0.54 +0.1 326,808 - MPMX20_00304 0.62 +0.0 326,808 - MPMX20_00304 0.62 -0.5 326,928 - MPMX20_00304 0.74 +0.6 326,928 - MPMX20_00304 0.74 -1.2 326,950 + MPMX20_00304 0.77 -1.5 326,950 + MPMX20_00304 0.77 -0.4 327,009 - MPMX20_00304 0.83 -0.8 327,009 - MPMX20_00304 0.83 +1.0 327,032 + MPMX20_00304 0.85 -0.2 327,032 + MPMX20_00304 0.85 -1.6 327,040 - MPMX20_00304 0.86 +1.0 327,075 - -0.7 327,077 + +0.4 327,085 - +0.0 327,085 - -1.1 327,089 + +0.8 327,096 + +0.9 327,096 + +1.5 327,104 - -2.0 327,114 - -0.5 327,116 + -0.8 327,137 - +3.2 327,141 - -0.4 327,178 + +0.1 327,249 + MPMX20_00305 0.14 +0.3 327,252 - MPMX20_00305 0.14 +0.8 327,254 + MPMX20_00305 0.15 +1.2 327,254 + MPMX20_00305 0.15 -0.8 327,254 + MPMX20_00305 0.15 +0.4 327,257 - MPMX20_00305 0.15 -0.2 327,262 - MPMX20_00305 0.16 -0.4 327,262 - MPMX20_00305 0.16 -0.5 327,351 - MPMX20_00305 0.28 +0.3 327,353 + MPMX20_00305 0.29 +1.7 327,354 + MPMX20_00305 0.29 -1.0 327,354 + MPMX20_00305 0.29 -1.2 327,373 + MPMX20_00305 0.32 +4.3 327,427 - MPMX20_00305 0.39 -0.2 327,436 - MPMX20_00305 0.41 +0.2 327,507 + MPMX20_00305 0.51 -0.9 327,607 + MPMX20_00305 0.65 +0.3 327,615 - MPMX20_00305 0.66 +1.1 327,615 - MPMX20_00305 0.66 -0.8 327,631 + MPMX20_00305 0.69 -0.4 327,634 - MPMX20_00305 0.69 +0.6 327,639 - MPMX20_00305 0.70 -1.7 327,666 + MPMX20_00305 0.74 -1.2 327,674 + MPMX20_00305 0.75 -1.0 327,674 - MPMX20_00305 0.75 +0.2 327,744 - MPMX20_00305 0.85 +1.2 327,762 + MPMX20_00305 0.88 +0.1 327,779 + -1.2 327,787 - +1.4 327,815 + -0.1 327,815 + -1.0 327,815 + +1.2 327,815 + +0.3 327,823 - +0.0 327,823 - -2.2 327,823 - +0.4 327,823 - +3.0 327,825 + +1.2 327,825 - +1.4 327,939 - -3.1 328,089 + MPMX20_00306 0.23 -0.8 328,097 + MPMX20_00306 0.24 +0.3 328,097 + MPMX20_00306 0.24 -1.0 328,111 + MPMX20_00306 0.26 +0.0 328,117 + MPMX20_00306 0.26 -1.4 328,126 + MPMX20_00306 0.27 -0.1 328,131 + MPMX20_00306 0.28 -0.1 328,131 + MPMX20_00306 0.28 +2.8 328,139 - MPMX20_00306 0.29 -2.9 328,153 + MPMX20_00306 0.30 +0.1
Or see this region's nucleotide sequence