Experiment: R2A with 3-octanone 1.77016666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS11295 and ABIE51_RS11300 overlap by 7 nucleotides ABIE51_RS11300 and ABIE51_RS11305 overlap by 74 nucleotides ABIE51_RS11305 and ABIE51_RS11310 are separated by 88 nucleotides ABIE51_RS11310 and ABIE51_RS11315 are separated by 294 nucleotides
ABIE51_RS11295: ABIE51_RS11295 - SDR family oxidoreductase, at 2,447,426 to 2,448,187
_RS11295
ABIE51_RS11300: ABIE51_RS11300 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 2,448,181 to 2,448,516
_RS11300
ABIE51_RS11305: ABIE51_RS11305 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 2,448,443 to 2,448,661
_RS11305
ABIE51_RS11310: ABIE51_RS11310 - hypothetical protein, at 2,448,750 to 2,449,361
_RS11310
ABIE51_RS11315: ABIE51_RS11315 - NADPH-dependent FMN reductase, at 2,449,656 to 2,450,225
_RS11315
Position (kb)
2448
2449 Strain fitness (log2 ratio)
-1
0
1 at 2447.566 kb on + strand, within ABIE51_RS11295 at 2447.808 kb on - strand, within ABIE51_RS11295 at 2447.913 kb on - strand, within ABIE51_RS11295 at 2448.183 kb on - strand at 2448.200 kb on + strand at 2448.200 kb on + strand at 2448.201 kb on - strand at 2448.201 kb on - strand at 2448.306 kb on - strand, within ABIE51_RS11300 at 2448.317 kb on - strand, within ABIE51_RS11300 at 2448.321 kb on - strand, within ABIE51_RS11300 at 2448.459 kb on + strand at 2448.459 kb on + strand at 2448.459 kb on + strand at 2448.459 kb on + strand at 2448.459 kb on + strand at 2448.830 kb on - strand, within ABIE51_RS11310 at 2448.911 kb on - strand, within ABIE51_RS11310 at 2448.934 kb on + strand, within ABIE51_RS11310 at 2448.934 kb on + strand, within ABIE51_RS11310 at 2448.935 kb on - strand, within ABIE51_RS11310 at 2449.163 kb on - strand, within ABIE51_RS11310 at 2449.240 kb on + strand, within ABIE51_RS11310 at 2449.241 kb on - strand, within ABIE51_RS11310 at 2449.374 kb on + strand at 2449.374 kb on + strand at 2449.375 kb on - strand at 2449.375 kb on - strand at 2449.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 1.77016666666667 mM remove 2,447,566 + ABIE51_RS11295 0.18 +0.8 2,447,808 - ABIE51_RS11295 0.50 -0.3 2,447,913 - ABIE51_RS11295 0.64 +0.3 2,448,183 - -0.2 2,448,200 + +0.0 2,448,200 + -0.0 2,448,201 - +0.8 2,448,201 - -1.1 2,448,306 - ABIE51_RS11300 0.37 +1.0 2,448,317 - ABIE51_RS11300 0.40 +0.2 2,448,321 - ABIE51_RS11300 0.42 +0.9 2,448,459 + -0.0 2,448,459 + +0.0 2,448,459 + +0.4 2,448,459 + +0.4 2,448,459 + -0.1 2,448,830 - ABIE51_RS11310 0.13 -1.3 2,448,911 - ABIE51_RS11310 0.26 +1.2 2,448,934 + ABIE51_RS11310 0.30 +0.1 2,448,934 + ABIE51_RS11310 0.30 +0.7 2,448,935 - ABIE51_RS11310 0.30 -0.1 2,449,163 - ABIE51_RS11310 0.67 +0.1 2,449,240 + ABIE51_RS11310 0.80 -0.1 2,449,241 - ABIE51_RS11310 0.80 -0.1 2,449,374 + -0.3 2,449,374 + +0.6 2,449,375 - +0.6 2,449,375 - +0.3 2,449,375 - -0.0
Or see this region's nucleotide sequence