Experiment: R2A with 3-octanone 2.65516666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS14475 and ABIE51_RS14480 are separated by 10 nucleotides ABIE51_RS14480 and ABIE51_RS14485 overlap by 4 nucleotides ABIE51_RS14485 and ABIE51_RS14490 are separated by 107 nucleotides
ABIE51_RS14475: ABIE51_RS14475 - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 3,144,351 to 3,146,615
_RS14475
ABIE51_RS14480: ABIE51_RS14480 - hypothetical protein, at 3,146,626 to 3,147,156
_RS14480
ABIE51_RS14485: ABIE51_RS14485 - MOSC domain-containing protein, at 3,147,153 to 3,147,668
_RS14485
ABIE51_RS14490: ABIE51_RS14490 - N-acetylmuramoyl-L-alanine amidase, at 3,147,776 to 3,148,348
_RS14490
Position (kb)
3146
3147
3148 Strain fitness (log2 ratio)
-1
0
1
2 at 3146.047 kb on + strand, within ABIE51_RS14475 at 3146.047 kb on + strand, within ABIE51_RS14475 at 3146.047 kb on + strand, within ABIE51_RS14475 at 3146.047 kb on + strand, within ABIE51_RS14475 at 3146.047 kb on + strand, within ABIE51_RS14475 at 3146.048 kb on - strand, within ABIE51_RS14475 at 3146.617 kb on + strand at 3146.617 kb on + strand at 3146.617 kb on + strand at 3146.617 kb on + strand at 3146.617 kb on + strand at 3146.617 kb on + strand at 3146.618 kb on - strand at 3146.618 kb on - strand at 3146.618 kb on - strand at 3146.622 kb on - strand at 3146.730 kb on - strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.900 kb on + strand, within ABIE51_RS14480 at 3146.901 kb on - strand, within ABIE51_RS14480 at 3146.987 kb on + strand, within ABIE51_RS14480 at 3146.987 kb on + strand, within ABIE51_RS14480 at 3146.988 kb on - strand, within ABIE51_RS14480 at 3147.035 kb on + strand, within ABIE51_RS14480 at 3147.035 kb on + strand, within ABIE51_RS14480 at 3147.505 kb on + strand, within ABIE51_RS14485 at 3147.762 kb on + strand at 3147.762 kb on + strand at 3147.763 kb on - strand at 3147.824 kb on + strand at 3147.825 kb on - strand at 3147.971 kb on + strand, within ABIE51_RS14490 at 3147.971 kb on + strand, within ABIE51_RS14490 at 3147.971 kb on + strand, within ABIE51_RS14490 at 3147.971 kb on + strand, within ABIE51_RS14490 at 3147.972 kb on - strand, within ABIE51_RS14490 at 3147.972 kb on - strand, within ABIE51_RS14490 at 3147.972 kb on - strand, within ABIE51_RS14490 at 3147.972 kb on - strand, within ABIE51_RS14490 at 3148.001 kb on + strand, within ABIE51_RS14490 at 3148.002 kb on - strand, within ABIE51_RS14490 at 3148.002 kb on - strand, within ABIE51_RS14490 at 3148.002 kb on - strand, within ABIE51_RS14490 at 3148.002 kb on - strand, within ABIE51_RS14490 at 3148.086 kb on - strand, within ABIE51_RS14490 at 3148.130 kb on + strand, within ABIE51_RS14490 at 3148.131 kb on - strand, within ABIE51_RS14490 at 3148.131 kb on - strand, within ABIE51_RS14490 at 3148.131 kb on - strand, within ABIE51_RS14490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 2.65516666666667 mM remove 3,146,047 + ABIE51_RS14475 0.75 +0.3 3,146,047 + ABIE51_RS14475 0.75 +0.3 3,146,047 + ABIE51_RS14475 0.75 +0.2 3,146,047 + ABIE51_RS14475 0.75 +0.4 3,146,047 + ABIE51_RS14475 0.75 -0.4 3,146,048 - ABIE51_RS14475 0.75 +0.9 3,146,617 + -1.0 3,146,617 + +0.2 3,146,617 + -0.5 3,146,617 + -0.2 3,146,617 + +0.3 3,146,617 + -0.0 3,146,618 - +2.1 3,146,618 - +1.0 3,146,618 - -0.2 3,146,622 - -0.7 3,146,730 - ABIE51_RS14480 0.20 +0.2 3,146,900 + ABIE51_RS14480 0.52 -0.3 3,146,900 + ABIE51_RS14480 0.52 -0.9 3,146,900 + ABIE51_RS14480 0.52 +0.0 3,146,900 + ABIE51_RS14480 0.52 -0.3 3,146,900 + ABIE51_RS14480 0.52 -1.7 3,146,900 + ABIE51_RS14480 0.52 +0.2 3,146,900 + ABIE51_RS14480 0.52 -0.3 3,146,901 - ABIE51_RS14480 0.52 -0.3 3,146,987 + ABIE51_RS14480 0.68 +0.3 3,146,987 + ABIE51_RS14480 0.68 +1.0 3,146,988 - ABIE51_RS14480 0.68 -1.2 3,147,035 + ABIE51_RS14480 0.77 +0.3 3,147,035 + ABIE51_RS14480 0.77 +0.3 3,147,505 + ABIE51_RS14485 0.68 -0.5 3,147,762 + -0.4 3,147,762 + +0.2 3,147,763 - -0.2 3,147,824 + -0.0 3,147,825 - -0.3 3,147,971 + ABIE51_RS14490 0.34 -0.0 3,147,971 + ABIE51_RS14490 0.34 +0.3 3,147,971 + ABIE51_RS14490 0.34 -0.7 3,147,971 + ABIE51_RS14490 0.34 -0.4 3,147,972 - ABIE51_RS14490 0.34 -0.9 3,147,972 - ABIE51_RS14490 0.34 -0.5 3,147,972 - ABIE51_RS14490 0.34 -0.2 3,147,972 - ABIE51_RS14490 0.34 +0.1 3,148,001 + ABIE51_RS14490 0.39 -0.6 3,148,002 - ABIE51_RS14490 0.39 +0.3 3,148,002 - ABIE51_RS14490 0.39 -0.1 3,148,002 - ABIE51_RS14490 0.39 -0.9 3,148,002 - ABIE51_RS14490 0.39 -1.2 3,148,086 - ABIE51_RS14490 0.54 +0.5 3,148,130 + ABIE51_RS14490 0.62 +0.2 3,148,131 - ABIE51_RS14490 0.62 -0.0 3,148,131 - ABIE51_RS14490 0.62 +0.3 3,148,131 - ABIE51_RS14490 0.62 -0.7
Or see this region's nucleotide sequence