Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08580 and PGA1_c08590 are separated by 94 nucleotides PGA1_c08590 and PGA1_c08600 overlap by 1 nucleotides PGA1_c08600 and PGA1_c08610 are separated by 261 nucleotides PGA1_c08610 and PGA1_c08620 are separated by 335 nucleotides PGA1_c08620 and PGA1_c08630 are separated by 144 nucleotides
PGA1_c08580: PGA1_c08580 - hypothetical protein, at 885,167 to 886,957
_c08580
PGA1_c08590: PGA1_c08590 - heme NO binding domain-containing protein, at 887,052 to 887,639
_c08590
PGA1_c08600: PGA1_c08600 - diguanylate cyclase (GGDEF) domain-containing protein, at 887,639 to 888,640
_c08600
PGA1_c08610: PGA1_c08610 - tRNA-Glu, at 888,902 to 888,976
_c08610
PGA1_c08620: PGA1_c08620 - tRNA-Glu, at 889,312 to 889,386
_c08620
PGA1_c08630: PGA1_c08630 - single-stranded-DNA-specific exonuclease RecJ, at 889,531 to 891,273
_c08630
Position (kb) 887
888
889 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 886.689 kb on + strand, within PGA1_c08580 at 886.697 kb on - strand, within PGA1_c08580 at 886.741 kb on - strand, within PGA1_c08580 at 886.748 kb on + strand, within PGA1_c08580 at 886.756 kb on - strand, within PGA1_c08580 at 886.756 kb on - strand, within PGA1_c08580 at 886.758 kb on + strand, within PGA1_c08580 at 886.758 kb on + strand, within PGA1_c08580 at 886.758 kb on + strand, within PGA1_c08580 at 886.775 kb on + strand, within PGA1_c08580 at 886.785 kb on + strand at 886.785 kb on + strand at 886.793 kb on + strand at 886.793 kb on - strand at 886.793 kb on - strand at 886.801 kb on - strand at 886.801 kb on - strand at 886.811 kb on - strand at 886.819 kb on + strand at 886.827 kb on - strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.852 kb on + strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.860 kb on - strand at 886.887 kb on - strand at 886.897 kb on + strand at 886.917 kb on + strand at 886.932 kb on + strand at 886.932 kb on + strand at 886.932 kb on + strand at 886.932 kb on + strand at 886.940 kb on + strand at 886.940 kb on - strand at 886.940 kb on - strand at 886.945 kb on + strand at 886.947 kb on - strand at 886.950 kb on + strand at 886.951 kb on + strand at 886.953 kb on + strand at 886.953 kb on - strand at 886.959 kb on - strand at 886.966 kb on - strand at 887.010 kb on - strand at 887.010 kb on - strand at 887.025 kb on - strand at 887.032 kb on + strand at 887.049 kb on + strand at 887.052 kb on - strand at 887.057 kb on - strand at 887.063 kb on + strand at 887.076 kb on + strand at 887.131 kb on + strand, within PGA1_c08590 at 887.171 kb on + strand, within PGA1_c08590 at 887.179 kb on + strand, within PGA1_c08590 at 887.184 kb on - strand, within PGA1_c08590 at 887.217 kb on - strand, within PGA1_c08590 at 887.219 kb on - strand, within PGA1_c08590 at 887.219 kb on - strand, within PGA1_c08590 at 887.219 kb on - strand, within PGA1_c08590 at 887.293 kb on - strand, within PGA1_c08590 at 887.376 kb on - strand, within PGA1_c08590 at 887.388 kb on + strand, within PGA1_c08590 at 887.388 kb on + strand, within PGA1_c08590 at 887.396 kb on - strand, within PGA1_c08590 at 887.396 kb on - strand, within PGA1_c08590 at 887.396 kb on - strand, within PGA1_c08590 at 887.396 kb on - strand, within PGA1_c08590 at 887.466 kb on + strand, within PGA1_c08590 at 887.466 kb on + strand, within PGA1_c08590 at 887.489 kb on + strand, within PGA1_c08590 at 887.513 kb on - strand, within PGA1_c08590 at 887.552 kb on + strand, within PGA1_c08590 at 887.552 kb on + strand, within PGA1_c08590 at 887.555 kb on - strand, within PGA1_c08590 at 887.555 kb on - strand, within PGA1_c08590 at 887.557 kb on + strand, within PGA1_c08590 at 887.557 kb on + strand, within PGA1_c08590 at 887.557 kb on + strand, within PGA1_c08590 at 887.557 kb on + strand, within PGA1_c08590 at 887.557 kb on + strand, within PGA1_c08590 at 887.557 kb on + strand, within PGA1_c08590 at 887.560 kb on - strand, within PGA1_c08590 at 887.564 kb on + strand, within PGA1_c08590 at 887.565 kb on - strand, within PGA1_c08590 at 887.565 kb on - strand, within PGA1_c08590 at 887.565 kb on - strand, within PGA1_c08590 at 887.572 kb on - strand, within PGA1_c08590 at 887.578 kb on + strand, within PGA1_c08590 at 887.619 kb on - strand at 887.634 kb on + strand at 887.646 kb on - strand at 887.810 kb on + strand, within PGA1_c08600 at 887.814 kb on - strand, within PGA1_c08600 at 887.818 kb on - strand, within PGA1_c08600 at 887.836 kb on + strand, within PGA1_c08600 at 887.836 kb on + strand, within PGA1_c08600 at 887.836 kb on + strand, within PGA1_c08600 at 887.836 kb on + strand, within PGA1_c08600 at 887.844 kb on - strand, within PGA1_c08600 at 887.844 kb on - strand, within PGA1_c08600 at 887.844 kb on - strand, within PGA1_c08600 at 887.858 kb on - strand, within PGA1_c08600 at 887.923 kb on + strand, within PGA1_c08600 at 887.923 kb on + strand, within PGA1_c08600 at 887.931 kb on - strand, within PGA1_c08600 at 887.965 kb on + strand, within PGA1_c08600 at 887.973 kb on - strand, within PGA1_c08600 at 887.975 kb on + strand, within PGA1_c08600 at 887.990 kb on - strand, within PGA1_c08600 at 888.017 kb on + strand, within PGA1_c08600 at 888.017 kb on + strand, within PGA1_c08600 at 888.017 kb on + strand, within PGA1_c08600 at 888.017 kb on + strand, within PGA1_c08600 at 888.025 kb on - strand, within PGA1_c08600 at 888.080 kb on - strand, within PGA1_c08600 at 888.081 kb on + strand, within PGA1_c08600 at 888.081 kb on + strand, within PGA1_c08600 at 888.089 kb on - strand, within PGA1_c08600 at 888.127 kb on + strand, within PGA1_c08600 at 888.149 kb on + strand, within PGA1_c08600 at 888.149 kb on + strand, within PGA1_c08600 at 888.149 kb on + strand, within PGA1_c08600 at 888.149 kb on + strand, within PGA1_c08600 at 888.149 kb on + strand, within PGA1_c08600 at 888.157 kb on - strand, within PGA1_c08600 at 888.258 kb on - strand, within PGA1_c08600 at 888.288 kb on - strand, within PGA1_c08600 at 888.288 kb on - strand, within PGA1_c08600 at 888.306 kb on + strand, within PGA1_c08600 at 888.368 kb on + strand, within PGA1_c08600 at 888.521 kb on + strand, within PGA1_c08600 at 888.522 kb on + strand, within PGA1_c08600 at 888.536 kb on + strand, within PGA1_c08600 at 888.537 kb on + strand, within PGA1_c08600 at 888.592 kb on - strand at 888.635 kb on + strand at 888.636 kb on - strand at 888.677 kb on - strand at 888.695 kb on - strand at 888.723 kb on + strand at 888.723 kb on + strand at 888.723 kb on + strand at 888.723 kb on + strand at 888.731 kb on - strand at 888.769 kb on - strand at 888.769 kb on - strand at 888.843 kb on + strand at 888.843 kb on + strand at 888.851 kb on - strand at 888.857 kb on - strand at 888.885 kb on - strand at 888.893 kb on - strand at 888.893 kb on - strand at 889.183 kb on + strand at 889.183 kb on + strand at 889.490 kb on + strand at 889.491 kb on + strand at 889.506 kb on - strand at 889.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 886,689 + PGA1_c08580 0.85 +0.3 886,697 - PGA1_c08580 0.85 +0.5 886,741 - PGA1_c08580 0.88 +0.9 886,748 + PGA1_c08580 0.88 -0.2 886,756 - PGA1_c08580 0.89 -0.4 886,756 - PGA1_c08580 0.89 -0.2 886,758 + PGA1_c08580 0.89 -0.3 886,758 + PGA1_c08580 0.89 -0.5 886,758 + PGA1_c08580 0.89 -1.4 886,775 + PGA1_c08580 0.90 +0.3 886,785 + +0.3 886,785 + +0.7 886,793 + +0.3 886,793 - +0.0 886,793 - -0.1 886,801 - -0.7 886,801 - -0.9 886,811 - -1.0 886,819 + +0.3 886,827 - -0.7 886,852 + -0.7 886,852 + -3.0 886,852 + +0.7 886,852 + -0.2 886,852 + -1.0 886,852 + +0.3 886,852 + -0.3 886,852 + -0.5 886,852 + +0.7 886,852 + -0.7 886,852 + +0.6 886,860 - -1.1 886,860 - +0.3 886,860 - +1.5 886,860 - -0.8 886,860 - -0.2 886,860 - +0.8 886,860 - +0.4 886,860 - +0.3 886,860 - -0.1 886,887 - +0.2 886,897 + +0.9 886,917 + -0.3 886,932 + -0.7 886,932 + -0.2 886,932 + +0.1 886,932 + +1.4 886,940 + -0.2 886,940 - -0.8 886,940 - -1.5 886,945 + +0.4 886,947 - +0.4 886,950 + -1.0 886,951 + +0.6 886,953 + +1.2 886,953 - -0.2 886,959 - +0.1 886,966 - +0.2 887,010 - -0.1 887,010 - +0.4 887,025 - -1.0 887,032 + -1.8 887,049 + -1.5 887,052 - +0.3 887,057 - -1.7 887,063 + -0.8 887,076 + -0.3 887,131 + PGA1_c08590 0.13 -0.6 887,171 + PGA1_c08590 0.20 -0.4 887,179 + PGA1_c08590 0.22 +0.0 887,184 - PGA1_c08590 0.22 -3.0 887,217 - PGA1_c08590 0.28 -0.5 887,219 - PGA1_c08590 0.28 -1.5 887,219 - PGA1_c08590 0.28 -0.0 887,219 - PGA1_c08590 0.28 -0.0 887,293 - PGA1_c08590 0.41 -1.2 887,376 - PGA1_c08590 0.55 -0.6 887,388 + PGA1_c08590 0.57 -0.5 887,388 + PGA1_c08590 0.57 -4.2 887,396 - PGA1_c08590 0.59 +0.5 887,396 - PGA1_c08590 0.59 -1.0 887,396 - PGA1_c08590 0.59 -1.0 887,396 - PGA1_c08590 0.59 +0.1 887,466 + PGA1_c08590 0.70 -0.3 887,466 + PGA1_c08590 0.70 +0.5 887,489 + PGA1_c08590 0.74 -1.6 887,513 - PGA1_c08590 0.78 -1.9 887,552 + PGA1_c08590 0.85 -1.4 887,552 + PGA1_c08590 0.85 -1.6 887,555 - PGA1_c08590 0.86 -1.1 887,555 - PGA1_c08590 0.86 -0.3 887,557 + PGA1_c08590 0.86 -2.9 887,557 + PGA1_c08590 0.86 -1.4 887,557 + PGA1_c08590 0.86 +0.2 887,557 + PGA1_c08590 0.86 -0.2 887,557 + PGA1_c08590 0.86 -1.7 887,557 + PGA1_c08590 0.86 -0.9 887,560 - PGA1_c08590 0.86 -0.9 887,564 + PGA1_c08590 0.87 -1.8 887,565 - PGA1_c08590 0.87 -2.9 887,565 - PGA1_c08590 0.87 -0.2 887,565 - PGA1_c08590 0.87 -1.3 887,572 - PGA1_c08590 0.88 -1.2 887,578 + PGA1_c08590 0.89 +0.1 887,619 - -1.8 887,634 + -0.7 887,646 - -0.4 887,810 + PGA1_c08600 0.17 -3.1 887,814 - PGA1_c08600 0.17 -1.8 887,818 - PGA1_c08600 0.18 -1.3 887,836 + PGA1_c08600 0.20 +0.3 887,836 + PGA1_c08600 0.20 -1.9 887,836 + PGA1_c08600 0.20 -1.6 887,836 + PGA1_c08600 0.20 -0.1 887,844 - PGA1_c08600 0.20 -0.6 887,844 - PGA1_c08600 0.20 -1.9 887,844 - PGA1_c08600 0.20 -3.3 887,858 - PGA1_c08600 0.22 -2.0 887,923 + PGA1_c08600 0.28 -0.7 887,923 + PGA1_c08600 0.28 -2.1 887,931 - PGA1_c08600 0.29 -1.4 887,965 + PGA1_c08600 0.33 -2.0 887,973 - PGA1_c08600 0.33 -1.6 887,975 + PGA1_c08600 0.34 -0.9 887,990 - PGA1_c08600 0.35 -0.7 888,017 + PGA1_c08600 0.38 -1.4 888,017 + PGA1_c08600 0.38 +0.3 888,017 + PGA1_c08600 0.38 -1.0 888,017 + PGA1_c08600 0.38 -1.3 888,025 - PGA1_c08600 0.39 -0.4 888,080 - PGA1_c08600 0.44 -0.5 888,081 + PGA1_c08600 0.44 -1.0 888,081 + PGA1_c08600 0.44 -0.6 888,089 - PGA1_c08600 0.45 -0.5 888,127 + PGA1_c08600 0.49 -1.2 888,149 + PGA1_c08600 0.51 -0.4 888,149 + PGA1_c08600 0.51 -0.4 888,149 + PGA1_c08600 0.51 -1.3 888,149 + PGA1_c08600 0.51 -1.8 888,149 + PGA1_c08600 0.51 -1.8 888,157 - PGA1_c08600 0.52 -1.4 888,258 - PGA1_c08600 0.62 -0.8 888,288 - PGA1_c08600 0.65 -1.7 888,288 - PGA1_c08600 0.65 -0.5 888,306 + PGA1_c08600 0.67 -0.1 888,368 + PGA1_c08600 0.73 -1.9 888,521 + PGA1_c08600 0.88 -0.6 888,522 + PGA1_c08600 0.88 -1.8 888,536 + PGA1_c08600 0.90 +0.0 888,537 + PGA1_c08600 0.90 +0.3 888,592 - +0.6 888,635 + +0.3 888,636 - -0.3 888,677 - +0.3 888,695 - -0.1 888,723 + +0.6 888,723 + -0.3 888,723 + -1.4 888,723 + +0.4 888,731 - +1.3 888,769 - +0.0 888,769 - -0.1 888,843 + -0.1 888,843 + +0.9 888,851 - -0.5 888,857 - -0.2 888,885 - -0.5 888,893 - -0.2 888,893 - +0.3 889,183 + -1.6 889,183 + +0.1 889,490 + -0.2 889,491 + -0.0 889,506 - -1.0 889,527 + +2.2
Or see this region's nucleotide sequence