Experiment: H2 N-free with 10mM L-Ornithine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03080 and MMP_RS03085 are separated by 4 nucleotides MMP_RS03085 and MMP_RS03090 are separated by 25 nucleotides MMP_RS03090 and MMP_RS03095 are separated by 116 nucleotides MMP_RS03095 and MMP_RS03100 are separated by 5 nucleotides
MMP_RS03080: MMP_RS03080 - universal stress protein, at 582,112 to 582,549
_RS03080
MMP_RS03085: MMP_RS03085 - endonuclease III, at 582,554 to 583,252
_RS03085
MMP_RS03090: MMP_RS03090 - cation:proton antiporter, at 583,278 to 584,447
_RS03090
MMP_RS03095: MMP_RS03095 - diphthine synthase, at 584,564 to 585,331
_RS03095
MMP_RS03100: MMP_RS03100 - hypothetical protein, at 585,337 to 585,537
_RS03100
Position (kb) 583
584
585 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 582.359 kb on - strand, within MMP_RS03080 at 582.360 kb on + strand, within MMP_RS03080 at 582.476 kb on - strand, within MMP_RS03080 at 582.477 kb on + strand, within MMP_RS03080 at 582.555 kb on - strand at 582.556 kb on + strand at 582.584 kb on - strand at 582.585 kb on + strand at 582.589 kb on + strand at 582.654 kb on + strand, within MMP_RS03085 at 582.737 kb on + strand, within MMP_RS03085 at 582.740 kb on - strand, within MMP_RS03085 at 582.740 kb on - strand, within MMP_RS03085 at 582.741 kb on + strand, within MMP_RS03085 at 582.755 kb on + strand, within MMP_RS03085 at 582.757 kb on + strand, within MMP_RS03085 at 582.869 kb on - strand, within MMP_RS03085 at 582.921 kb on - strand, within MMP_RS03085 at 582.938 kb on + strand, within MMP_RS03085 at 583.110 kb on - strand, within MMP_RS03085 at 583.365 kb on - strand at 583.396 kb on + strand, within MMP_RS03090 at 583.775 kb on - strand, within MMP_RS03090 at 583.786 kb on + strand, within MMP_RS03090 at 583.786 kb on + strand, within MMP_RS03090 at 583.792 kb on + strand, within MMP_RS03090 at 583.843 kb on + strand, within MMP_RS03090 at 583.845 kb on - strand, within MMP_RS03090 at 583.849 kb on + strand, within MMP_RS03090 at 583.889 kb on - strand, within MMP_RS03090 at 584.135 kb on + strand, within MMP_RS03090 at 584.468 kb on - strand at 584.468 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.516 kb on - strand at 584.516 kb on - strand at 584.516 kb on - strand at 584.517 kb on + strand at 584.518 kb on - strand at 584.518 kb on - strand at 584.519 kb on + strand at 584.519 kb on + strand at 584.522 kb on + strand at 584.540 kb on + strand at 584.546 kb on - strand at 584.547 kb on + strand at 584.592 kb on + strand at 584.609 kb on + strand at 584.609 kb on + strand at 584.673 kb on - strand, within MMP_RS03095 at 584.678 kb on + strand, within MMP_RS03095 at 584.678 kb on + strand, within MMP_RS03095 at 584.725 kb on + strand, within MMP_RS03095 at 584.754 kb on + strand, within MMP_RS03095 at 585.002 kb on - strand, within MMP_RS03095 at 585.071 kb on + strand, within MMP_RS03095 at 585.206 kb on - strand, within MMP_RS03095 at 585.210 kb on + strand, within MMP_RS03095 at 585.401 kb on + strand, within MMP_RS03100
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Ornithine remove 582,359 - MMP_RS03080 0.56 +0.8 582,360 + MMP_RS03080 0.57 -1.7 582,476 - MMP_RS03080 0.83 -1.6 582,477 + MMP_RS03080 0.83 -0.6 582,555 - -0.4 582,556 + +1.1 582,584 - +0.2 582,585 + +0.7 582,589 + -0.9 582,654 + MMP_RS03085 0.14 +0.9 582,737 + MMP_RS03085 0.26 -0.9 582,740 - MMP_RS03085 0.27 -0.0 582,740 - MMP_RS03085 0.27 +0.4 582,741 + MMP_RS03085 0.27 -0.9 582,755 + MMP_RS03085 0.29 +0.9 582,757 + MMP_RS03085 0.29 +0.7 582,869 - MMP_RS03085 0.45 -0.3 582,921 - MMP_RS03085 0.53 -0.2 582,938 + MMP_RS03085 0.55 +0.5 583,110 - MMP_RS03085 0.80 +0.2 583,365 - -1.2 583,396 + MMP_RS03090 0.10 -1.1 583,775 - MMP_RS03090 0.42 -0.9 583,786 + MMP_RS03090 0.43 -2.1 583,786 + MMP_RS03090 0.43 -0.5 583,792 + MMP_RS03090 0.44 -1.9 583,843 + MMP_RS03090 0.48 -1.0 583,845 - MMP_RS03090 0.48 -0.9 583,849 + MMP_RS03090 0.49 -1.7 583,889 - MMP_RS03090 0.52 -1.5 584,135 + MMP_RS03090 0.73 -3.1 584,468 - -0.4 584,468 - -1.2 584,514 - -0.7 584,514 - -0.5 584,514 - -1.8 584,514 - -1.2 584,514 - -0.6 584,514 - -1.1 584,514 - -1.7 584,514 - -1.0 584,514 - -0.8 584,514 - -0.1 584,515 + -3.5 584,515 + -1.2 584,515 + -0.5 584,515 + -1.1 584,515 + -0.8 584,515 + -1.0 584,515 + -1.0 584,515 + +0.2 584,516 - -1.1 584,516 - -1.1 584,516 - -1.2 584,517 + -1.4 584,518 - -1.8 584,518 - -0.5 584,519 + +0.3 584,519 + -0.4 584,522 + +0.2 584,540 + -0.3 584,546 - -0.1 584,547 + -1.0 584,592 + -2.1 584,609 + -1.8 584,609 + +0.3 584,673 - MMP_RS03095 0.14 -1.7 584,678 + MMP_RS03095 0.15 -0.9 584,678 + MMP_RS03095 0.15 -0.8 584,725 + MMP_RS03095 0.21 -1.0 584,754 + MMP_RS03095 0.25 -0.0 585,002 - MMP_RS03095 0.57 +0.4 585,071 + MMP_RS03095 0.66 -0.7 585,206 - MMP_RS03095 0.84 -0.7 585,210 + MMP_RS03095 0.84 -0.5 585,401 + MMP_RS03100 0.32 +0.7
Or see this region's nucleotide sequence