Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12870

Experiment: LB, pH10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotidesBWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotidesBWI76_RS12875 and BWI76_RS12880 are separated by 72 nucleotides BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022 _RS12865 BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861 _RS12870 BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557 _RS12875 BWI76_RS12880: BWI76_RS12880 - aspartate-semialdehyde dehydrogenase, at 2,683,630 to 2,684,748 _RS12880 Position (kb) 2682 2683Strain fitness (log2 ratio) -2 -1 0 1at 2681.110 kb on - strand, within BWI76_RS12865at 2681.268 kb on + strand, within BWI76_RS12865at 2681.368 kb on - strand, within BWI76_RS12865at 2681.368 kb on - strand, within BWI76_RS12865at 2681.518 kb on - strand, within BWI76_RS12865at 2681.906 kb on + strandat 2681.921 kb on - strandat 2682.117 kb on + strandat 2682.117 kb on + strandat 2682.205 kb on - strand, within BWI76_RS12870at 2682.302 kb on + strand, within BWI76_RS12870at 2682.418 kb on + strand, within BWI76_RS12870at 2682.797 kb on + strandat 2682.911 kb on + strandat 2683.019 kb on + strandat 2683.148 kb on - strand, within BWI76_RS12875at 2683.188 kb on + strand, within BWI76_RS12875at 2683.225 kb on - strand, within BWI76_RS12875at 2683.379 kb on - strand, within BWI76_RS12875at 2683.526 kb on - strandat 2683.568 kb on + strandat 2683.573 kb on - strandat 2683.576 kb on - strandat 2683.612 kb on - strandat 2683.663 kb on + strandat 2683.700 kb on + strandat 2683.713 kb on - strandat 2683.725 kb on + strandat 2683.791 kb on - strand, within BWI76_RS12880at 2683.801 kb on - strand, within BWI76_RS12880at 2683.811 kb on - strand, within BWI76_RS12880

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH10
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2,681,110 - BWI76_RS12865 0.22 +0.2
2,681,268 + BWI76_RS12865 0.35 -1.0
2,681,368 - BWI76_RS12865 0.44 -0.4
2,681,368 - BWI76_RS12865 0.44 +0.1
2,681,518 - BWI76_RS12865 0.57 +1.1
2,681,906 + +0.1
2,681,921 - -0.9
2,682,117 + +0.0
2,682,117 + -1.1
2,682,205 - BWI76_RS12870 0.19 +0.4
2,682,302 + BWI76_RS12870 0.31 -1.0
2,682,418 + BWI76_RS12870 0.46 -2.3
2,682,797 + -0.3
2,682,911 + +1.3
2,683,019 + -0.6
2,683,148 - BWI76_RS12875 0.24 +0.2
2,683,188 + BWI76_RS12875 0.31 -0.5
2,683,225 - BWI76_RS12875 0.38 -0.1
2,683,379 - BWI76_RS12875 0.67 -1.5
2,683,526 - +0.2
2,683,568 + +1.0
2,683,573 - +0.0
2,683,576 - +0.7
2,683,612 - +0.1
2,683,663 + +1.2
2,683,700 + -0.1
2,683,713 - +0.3
2,683,725 + +0.4
2,683,791 - BWI76_RS12880 0.14 -2.0
2,683,801 - BWI76_RS12880 0.15 -0.3
2,683,811 - BWI76_RS12880 0.16 -1.6

Or see this region's nucleotide sequence