Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotides BWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides BWI76_RS12875 and BWI76_RS12880 are separated by 72 nucleotides
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861
_RS12870
BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557
_RS12875
BWI76_RS12880: BWI76_RS12880 - aspartate-semialdehyde dehydrogenase, at 2,683,630 to 2,684,748
_RS12880
Position (kb)
2682
2683 Strain fitness (log2 ratio)
-2
-1
0
1 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865 at 2681.906 kb on + strand at 2681.921 kb on - strand at 2682.117 kb on + strand at 2682.117 kb on + strand at 2682.205 kb on - strand, within BWI76_RS12870 at 2682.302 kb on + strand, within BWI76_RS12870 at 2682.418 kb on + strand, within BWI76_RS12870 at 2682.797 kb on + strand at 2682.911 kb on + strand at 2683.019 kb on + strand at 2683.148 kb on - strand, within BWI76_RS12875 at 2683.188 kb on + strand, within BWI76_RS12875 at 2683.225 kb on - strand, within BWI76_RS12875 at 2683.379 kb on - strand, within BWI76_RS12875 at 2683.526 kb on - strand at 2683.568 kb on + strand at 2683.573 kb on - strand at 2683.576 kb on - strand at 2683.612 kb on - strand at 2683.663 kb on + strand at 2683.700 kb on + strand at 2683.713 kb on - strand at 2683.725 kb on + strand at 2683.791 kb on - strand, within BWI76_RS12880 at 2683.801 kb on - strand, within BWI76_RS12880 at 2683.811 kb on - strand, within BWI76_RS12880
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 2,681,110 - BWI76_RS12865 0.22 +0.2 2,681,268 + BWI76_RS12865 0.35 -1.0 2,681,368 - BWI76_RS12865 0.44 -0.4 2,681,368 - BWI76_RS12865 0.44 +0.1 2,681,518 - BWI76_RS12865 0.57 +1.1 2,681,906 + +0.1 2,681,921 - -0.9 2,682,117 + +0.0 2,682,117 + -1.1 2,682,205 - BWI76_RS12870 0.19 +0.4 2,682,302 + BWI76_RS12870 0.31 -1.0 2,682,418 + BWI76_RS12870 0.46 -2.3 2,682,797 + -0.3 2,682,911 + +1.3 2,683,019 + -0.6 2,683,148 - BWI76_RS12875 0.24 +0.2 2,683,188 + BWI76_RS12875 0.31 -0.5 2,683,225 - BWI76_RS12875 0.38 -0.1 2,683,379 - BWI76_RS12875 0.67 -1.5 2,683,526 - +0.2 2,683,568 + +1.0 2,683,573 - +0.0 2,683,576 - +0.7 2,683,612 - +0.1 2,683,663 + +1.2 2,683,700 + -0.1 2,683,713 - +0.3 2,683,725 + +0.4 2,683,791 - BWI76_RS12880 0.14 -2.0 2,683,801 - BWI76_RS12880 0.15 -0.3 2,683,811 - BWI76_RS12880 0.16 -1.6
Or see this region's nucleotide sequence