Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04950 and BWI76_RS04955 are separated by 203 nucleotides BWI76_RS04955 and BWI76_RS04960 are separated by 50 nucleotides BWI76_RS04960 and BWI76_RS04965 are separated by 107 nucleotides
BWI76_RS04950: BWI76_RS04950 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 1,038,990 to 1,041,380
_RS04950
BWI76_RS04955: BWI76_RS04955 - hypoxanthine phosphoribosyltransferase, at 1,041,584 to 1,042,120
_RS04955
BWI76_RS04960: BWI76_RS04960 - carbonate dehydratase, at 1,042,171 to 1,042,833
_RS04960
BWI76_RS04965: BWI76_RS04965 - ABC transporter ATP-binding protein, at 1,042,941 to 1,043,867
_RS04965
Position (kb) 1041
1042
1043 Strain fitness (log2 ratio) -1
0
1
2 at 1040.694 kb on - strand, within BWI76_RS04950 at 1040.852 kb on - strand, within BWI76_RS04950 at 1040.873 kb on - strand, within BWI76_RS04950 at 1041.073 kb on - strand, within BWI76_RS04950 at 1041.210 kb on - strand at 1041.258 kb on - strand at 1041.498 kb on + strand at 1041.749 kb on + strand, within BWI76_RS04955 at 1041.801 kb on - strand, within BWI76_RS04955 at 1042.160 kb on - strand at 1042.160 kb on - strand at 1042.279 kb on + strand, within BWI76_RS04960 at 1042.303 kb on - strand at 1042.491 kb on + strand, within BWI76_RS04960 at 1042.585 kb on + strand, within BWI76_RS04960 at 1042.624 kb on - strand, within BWI76_RS04960 at 1042.785 kb on + strand at 1043.048 kb on + strand, within BWI76_RS04965
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 1,040,694 - BWI76_RS04950 0.71 -0.8 1,040,852 - BWI76_RS04950 0.78 +0.1 1,040,873 - BWI76_RS04950 0.79 -0.3 1,041,073 - BWI76_RS04950 0.87 -0.3 1,041,210 - +0.7 1,041,258 - -0.4 1,041,498 + +0.1 1,041,749 + BWI76_RS04955 0.31 -1.2 1,041,801 - BWI76_RS04955 0.40 -0.6 1,042,160 - -1.5 1,042,160 - +0.0 1,042,279 + BWI76_RS04960 0.16 -0.0 1,042,303 - +2.5 1,042,491 + BWI76_RS04960 0.48 -0.5 1,042,585 + BWI76_RS04960 0.62 -0.4 1,042,624 - BWI76_RS04960 0.68 +0.3 1,042,785 + -0.8 1,043,048 + BWI76_RS04965 0.12 +0.1
Or see this region's nucleotide sequence