Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=48hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_05070 and IAI47_05075 are separated by 174 nucleotides IAI47_05075 and IAI47_05080 are separated by 62 nucleotides IAI47_05080 and IAI47_05085 are separated by 310 nucleotides
IAI47_05070: IAI47_05070 - outer membrane protein assembly factor BamB, at 1,070,138 to 1,071,319
_05070
IAI47_05075: IAI47_05075 - ribosome biogenesis GTPase Der, at 1,071,494 to 1,072,984
_05075
IAI47_05080: IAI47_05080 - zinc ribbon domain-containing protein, at 1,073,047 to 1,073,268
_05080
IAI47_05085: IAI47_05085 - glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase, at 1,073,579 to 1,076,020
_05085
Position (kb)
1071
1072
1073 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1071.369 kb on + strand at 1071.369 kb on + strand at 1071.369 kb on + strand at 1071.370 kb on - strand at 1071.404 kb on + strand at 1071.404 kb on + strand at 1071.404 kb on + strand at 1071.405 kb on - strand at 1071.405 kb on - strand at 1071.405 kb on - strand at 1071.405 kb on - strand at 1071.418 kb on + strand at 1071.418 kb on + strand at 1071.418 kb on + strand at 1071.430 kb on + strand at 1071.446 kb on + strand at 1071.448 kb on + strand at 1072.975 kb on + strand at 1073.103 kb on + strand, within IAI47_05080 at 1073.128 kb on + strand, within IAI47_05080 at 1073.129 kb on - strand, within IAI47_05080 at 1073.174 kb on + strand, within IAI47_05080 at 1073.200 kb on + strand, within IAI47_05080 at 1073.200 kb on + strand, within IAI47_05080 at 1073.243 kb on + strand, within IAI47_05080 at 1073.243 kb on + strand, within IAI47_05080 at 1073.243 kb on + strand, within IAI47_05080 at 1073.244 kb on - strand, within IAI47_05080 at 1073.244 kb on - strand, within IAI47_05080 at 1073.244 kb on - strand, within IAI47_05080 at 1073.266 kb on + strand at 1073.282 kb on + strand at 1073.282 kb on + strand at 1073.283 kb on - strand at 1073.283 kb on - strand at 1073.288 kb on + strand at 1073.288 kb on + strand at 1073.288 kb on + strand at 1073.289 kb on - strand at 1073.289 kb on - strand at 1073.289 kb on - strand at 1073.349 kb on + strand at 1073.365 kb on + strand at 1073.365 kb on + strand at 1073.366 kb on - strand at 1073.366 kb on - strand at 1073.366 kb on - strand at 1073.366 kb on - strand at 1073.366 kb on - strand at 1073.394 kb on - strand at 1073.394 kb on - strand at 1073.474 kb on + strand at 1073.474 kb on + strand at 1073.474 kb on + strand at 1073.474 kb on + strand at 1073.474 kb on + strand at 1073.474 kb on + strand at 1073.476 kb on + strand at 1073.477 kb on - strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.540 kb on + strand at 1073.541 kb on - strand at 1073.541 kb on - strand at 1073.541 kb on - strand at 1073.541 kb on - strand at 1073.541 kb on - strand at 1073.541 kb on - strand at 1073.559 kb on + strand at 1073.559 kb on + strand at 1073.571 kb on + strand at 1073.571 kb on + strand at 1073.571 kb on + strand at 1073.571 kb on + strand at 1073.572 kb on - strand at 1073.572 kb on - strand at 1073.578 kb on + strand at 1073.578 kb on + strand at 1073.579 kb on - strand at 1073.711 kb on + strand at 1073.728 kb on + strand at 1073.729 kb on - strand at 1073.734 kb on + strand at 1073.734 kb on + strand at 1073.734 kb on + strand at 1073.734 kb on + strand at 1073.735 kb on - strand at 1073.735 kb on - strand at 1073.735 kb on - strand at 1073.735 kb on - strand at 1073.923 kb on + strand, within IAI47_05085 at 1073.924 kb on - strand, within IAI47_05085 at 1073.924 kb on - strand, within IAI47_05085 at 1073.924 kb on - strand, within IAI47_05085 at 1073.936 kb on - strand, within IAI47_05085 at 1073.936 kb on - strand, within IAI47_05085 at 1073.960 kb on + strand, within IAI47_05085 at 1073.961 kb on - strand, within IAI47_05085
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=48hrs; Outgrowth=LBkan50 remove 1,071,369 + -0.7 1,071,369 + +0.9 1,071,369 + +0.5 1,071,370 - +0.6 1,071,404 + -2.4 1,071,404 + -0.7 1,071,404 + +0.5 1,071,405 - -0.9 1,071,405 - -0.7 1,071,405 - -0.3 1,071,405 - -0.6 1,071,418 + +0.6 1,071,418 + -0.0 1,071,418 + -0.3 1,071,430 + -1.5 1,071,446 + +1.1 1,071,448 + +3.1 1,072,975 + +1.1 1,073,103 + IAI47_05080 0.25 -1.0 1,073,128 + IAI47_05080 0.36 +0.0 1,073,129 - IAI47_05080 0.37 -0.3 1,073,174 + IAI47_05080 0.57 -1.1 1,073,200 + IAI47_05080 0.69 -0.2 1,073,200 + IAI47_05080 0.69 -1.5 1,073,243 + IAI47_05080 0.88 -2.6 1,073,243 + IAI47_05080 0.88 -1.3 1,073,243 + IAI47_05080 0.88 -0.4 1,073,244 - IAI47_05080 0.89 -1.5 1,073,244 - IAI47_05080 0.89 +0.3 1,073,244 - IAI47_05080 0.89 -0.3 1,073,266 + +1.0 1,073,282 + +0.2 1,073,282 + -0.5 1,073,283 - -0.8 1,073,283 - +2.5 1,073,288 + -0.4 1,073,288 + -1.1 1,073,288 + +0.6 1,073,289 - -1.4 1,073,289 - +2.3 1,073,289 - -1.0 1,073,349 + -0.4 1,073,365 + -0.0 1,073,365 + -0.0 1,073,366 - -0.6 1,073,366 - +0.0 1,073,366 - +0.5 1,073,366 - +2.7 1,073,366 - -0.9 1,073,394 - -1.6 1,073,394 - -0.6 1,073,474 + -2.3 1,073,474 + +0.1 1,073,474 + +2.4 1,073,474 + -0.7 1,073,474 + +0.3 1,073,474 + -0.8 1,073,476 + -0.4 1,073,477 - +0.7 1,073,540 + -2.2 1,073,540 + -2.4 1,073,540 + -0.3 1,073,540 + -0.4 1,073,540 + -0.5 1,073,540 + -0.4 1,073,540 + +1.7 1,073,540 + -0.9 1,073,541 - +0.3 1,073,541 - -1.3 1,073,541 - -0.8 1,073,541 - +0.4 1,073,541 - +0.6 1,073,541 - -0.4 1,073,559 + +0.7 1,073,559 + -0.1 1,073,571 + -0.8 1,073,571 + -0.9 1,073,571 + -0.9 1,073,571 + +1.0 1,073,572 - +0.7 1,073,572 - -1.4 1,073,578 + -0.3 1,073,578 + -0.1 1,073,579 - -0.9 1,073,711 + -0.8 1,073,728 + -2.0 1,073,729 - -1.4 1,073,734 + +0.3 1,073,734 + -0.4 1,073,734 + -0.4 1,073,734 + -0.5 1,073,735 - -0.6 1,073,735 - +0.5 1,073,735 - -0.0 1,073,735 - +0.1 1,073,923 + IAI47_05085 0.14 +0.4 1,073,924 - IAI47_05085 0.14 -0.9 1,073,924 - IAI47_05085 0.14 +1.7 1,073,924 - IAI47_05085 0.14 -0.9 1,073,936 - IAI47_05085 0.15 +1.1 1,073,936 - IAI47_05085 0.15 +0.0 1,073,960 + IAI47_05085 0.16 -0.6 1,073,961 - IAI47_05085 0.16 -0.4
Or see this region's nucleotide sequence