Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14950 and PGA1_c14960 are separated by 65 nucleotides PGA1_c14960 and PGA1_c14970 are separated by 153 nucleotides PGA1_c14970 and PGA1_c14980 are separated by 136 nucleotides
PGA1_c14950: PGA1_c14950 - putative long-chain-fatty-acid-CoA ligase, at 1,554,361 to 1,555,968
_c14950
PGA1_c14960: PGA1_c14960 - putative transcriptional regulator, at 1,556,034 to 1,556,657
_c14960
PGA1_c14970: PGA1_c14970 - hypothetical protein, at 1,556,811 to 1,557,041
_c14970
PGA1_c14980: PGA1_c14980 - putative major intrinsic protein, at 1,557,178 to 1,557,834
_c14980
Position (kb)
1556
1557 Strain fitness (log2 ratio)
-2
-1
0
1 at 1555.085 kb on + strand, within PGA1_c14950 at 1555.182 kb on - strand, within PGA1_c14950 at 1555.276 kb on - strand, within PGA1_c14950 at 1555.281 kb on - strand, within PGA1_c14950 at 1555.309 kb on + strand, within PGA1_c14950 at 1555.309 kb on + strand, within PGA1_c14950 at 1555.345 kb on + strand, within PGA1_c14950 at 1555.363 kb on + strand, within PGA1_c14950 at 1555.516 kb on - strand, within PGA1_c14950 at 1555.559 kb on + strand, within PGA1_c14950 at 1555.559 kb on + strand, within PGA1_c14950 at 1555.579 kb on - strand, within PGA1_c14950 at 1555.587 kb on + strand, within PGA1_c14950 at 1555.602 kb on + strand, within PGA1_c14950 at 1555.605 kb on + strand, within PGA1_c14950 at 1555.605 kb on + strand, within PGA1_c14950 at 1555.613 kb on - strand, within PGA1_c14950 at 1555.629 kb on + strand, within PGA1_c14950 at 1555.695 kb on - strand, within PGA1_c14950 at 1555.747 kb on - strand, within PGA1_c14950 at 1555.780 kb on - strand, within PGA1_c14950 at 1555.780 kb on - strand, within PGA1_c14950 at 1555.831 kb on - strand at 1555.923 kb on + strand at 1555.923 kb on + strand at 1555.923 kb on + strand at 1555.961 kb on + strand at 1556.222 kb on + strand, within PGA1_c14960 at 1556.233 kb on + strand, within PGA1_c14960 at 1556.249 kb on - strand, within PGA1_c14960 at 1556.320 kb on - strand, within PGA1_c14960 at 1556.341 kb on - strand, within PGA1_c14960 at 1556.351 kb on - strand, within PGA1_c14960 at 1556.355 kb on + strand, within PGA1_c14960 at 1556.357 kb on - strand, within PGA1_c14960 at 1556.522 kb on + strand, within PGA1_c14960 at 1556.666 kb on - strand at 1556.704 kb on + strand at 1556.744 kb on + strand at 1556.753 kb on + strand at 1556.761 kb on + strand at 1556.761 kb on + strand at 1556.761 kb on + strand at 1556.761 kb on + strand at 1556.761 kb on + strand at 1556.769 kb on - strand at 1556.769 kb on - strand at 1556.769 kb on - strand at 1556.769 kb on - strand at 1556.769 kb on - strand at 1556.804 kb on + strand at 1556.804 kb on + strand at 1556.804 kb on + strand at 1556.809 kb on + strand at 1556.812 kb on - strand at 1556.812 kb on - strand at 1556.812 kb on - strand at 1556.851 kb on + strand, within PGA1_c14970 at 1556.859 kb on - strand, within PGA1_c14970 at 1556.859 kb on - strand, within PGA1_c14970 at 1557.037 kb on + strand at 1557.037 kb on + strand at 1557.037 kb on + strand at 1557.043 kb on + strand at 1557.045 kb on - strand at 1557.045 kb on - strand at 1557.164 kb on + strand at 1557.172 kb on - strand at 1557.172 kb on - strand at 1557.172 kb on - strand at 1557.219 kb on + strand at 1557.235 kb on - strand at 1557.255 kb on + strand, within PGA1_c14980 at 1557.258 kb on - strand, within PGA1_c14980 at 1557.263 kb on - strand, within PGA1_c14980 at 1557.266 kb on + strand, within PGA1_c14980 at 1557.274 kb on - strand, within PGA1_c14980 at 1557.274 kb on - strand, within PGA1_c14980 at 1557.274 kb on - strand, within PGA1_c14980 at 1557.274 kb on - strand, within PGA1_c14980 at 1557.353 kb on - strand, within PGA1_c14980 at 1557.387 kb on - strand, within PGA1_c14980 at 1557.387 kb on - strand, within PGA1_c14980 at 1557.395 kb on + strand, within PGA1_c14980 at 1557.395 kb on + strand, within PGA1_c14980 at 1557.480 kb on + strand, within PGA1_c14980 at 1557.489 kb on - strand, within PGA1_c14980 at 1557.569 kb on + strand, within PGA1_c14980 at 1557.569 kb on + strand, within PGA1_c14980 at 1557.577 kb on - strand, within PGA1_c14980 at 1557.598 kb on - strand, within PGA1_c14980 at 1557.619 kb on + strand, within PGA1_c14980 at 1557.637 kb on + strand, within PGA1_c14980
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 1,555,085 + PGA1_c14950 0.45 +0.3 1,555,182 - PGA1_c14950 0.51 -0.1 1,555,276 - PGA1_c14950 0.57 +0.3 1,555,281 - PGA1_c14950 0.57 +0.1 1,555,309 + PGA1_c14950 0.59 +1.1 1,555,309 + PGA1_c14950 0.59 -0.8 1,555,345 + PGA1_c14950 0.61 +0.5 1,555,363 + PGA1_c14950 0.62 +0.8 1,555,516 - PGA1_c14950 0.72 +0.0 1,555,559 + PGA1_c14950 0.75 -0.2 1,555,559 + PGA1_c14950 0.75 -1.0 1,555,579 - PGA1_c14950 0.76 +0.5 1,555,587 + PGA1_c14950 0.76 +1.0 1,555,602 + PGA1_c14950 0.77 +0.8 1,555,605 + PGA1_c14950 0.77 +1.1 1,555,605 + PGA1_c14950 0.77 -0.1 1,555,613 - PGA1_c14950 0.78 -0.3 1,555,629 + PGA1_c14950 0.79 -0.7 1,555,695 - PGA1_c14950 0.83 +0.8 1,555,747 - PGA1_c14950 0.86 +0.2 1,555,780 - PGA1_c14950 0.88 -1.1 1,555,780 - PGA1_c14950 0.88 +0.7 1,555,831 - -2.2 1,555,923 + +0.4 1,555,923 + -0.6 1,555,923 + -0.5 1,555,961 + -0.8 1,556,222 + PGA1_c14960 0.30 -1.1 1,556,233 + PGA1_c14960 0.32 +0.5 1,556,249 - PGA1_c14960 0.34 +1.6 1,556,320 - PGA1_c14960 0.46 +0.3 1,556,341 - PGA1_c14960 0.49 +0.3 1,556,351 - PGA1_c14960 0.51 +0.2 1,556,355 + PGA1_c14960 0.51 +0.2 1,556,357 - PGA1_c14960 0.52 +0.9 1,556,522 + PGA1_c14960 0.78 +0.7 1,556,666 - -0.3 1,556,704 + -1.6 1,556,744 + +0.4 1,556,753 + -0.0 1,556,761 + -0.5 1,556,761 + +0.1 1,556,761 + -0.3 1,556,761 + -1.2 1,556,761 + -0.1 1,556,769 - +0.9 1,556,769 - -0.4 1,556,769 - -0.8 1,556,769 - -0.9 1,556,769 - -0.5 1,556,804 + +0.7 1,556,804 + +0.2 1,556,804 + +0.0 1,556,809 + +1.1 1,556,812 - +0.7 1,556,812 - +0.3 1,556,812 - +0.1 1,556,851 + PGA1_c14970 0.17 +0.0 1,556,859 - PGA1_c14970 0.21 +0.1 1,556,859 - PGA1_c14970 0.21 -0.6 1,557,037 + +0.5 1,557,037 + +0.2 1,557,037 + -0.7 1,557,043 + -2.3 1,557,045 - -0.5 1,557,045 - +0.9 1,557,164 + -0.3 1,557,172 - -0.2 1,557,172 - -0.0 1,557,172 - -0.4 1,557,219 + +0.4 1,557,235 - +0.4 1,557,255 + PGA1_c14980 0.12 -0.8 1,557,258 - PGA1_c14980 0.12 +0.2 1,557,263 - PGA1_c14980 0.13 -0.1 1,557,266 + PGA1_c14980 0.13 +0.8 1,557,274 - PGA1_c14980 0.15 +1.0 1,557,274 - PGA1_c14980 0.15 -0.3 1,557,274 - PGA1_c14980 0.15 +0.2 1,557,274 - PGA1_c14980 0.15 -0.6 1,557,353 - PGA1_c14980 0.27 -0.3 1,557,387 - PGA1_c14980 0.32 -0.8 1,557,387 - PGA1_c14980 0.32 +0.1 1,557,395 + PGA1_c14980 0.33 +1.2 1,557,395 + PGA1_c14980 0.33 +1.7 1,557,480 + PGA1_c14980 0.46 -0.9 1,557,489 - PGA1_c14980 0.47 +0.3 1,557,569 + PGA1_c14980 0.60 -0.7 1,557,569 + PGA1_c14980 0.60 -1.5 1,557,577 - PGA1_c14980 0.61 +0.7 1,557,598 - PGA1_c14980 0.64 +0.1 1,557,619 + PGA1_c14980 0.67 +1.1 1,557,637 + PGA1_c14980 0.70 -1.0
Or see this region's nucleotide sequence