Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01330 and PGA1_c01340 are separated by 116 nucleotides PGA1_c01340 and PGA1_c01350 are separated by 193 nucleotides PGA1_c01350 and PGA1_c01360 are separated by 301 nucleotides PGA1_c01360 and PGA1_c01370 are separated by 243 nucleotides
PGA1_c01330: PGA1_c01330 - Uncharacterized protein conserved in bacteria, at 125,671 to 126,417
_c01330
PGA1_c01340: PGA1_c01340 - tRNA-Trp, at 126,534 to 126,609
_c01340
PGA1_c01350: PGA1_c01350 - putative UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase, at 126,803 to 128,995
_c01350
PGA1_c01360: PGA1_c01360 - putative preprotein translocase, secE subunit, at 129,297 to 129,494
_c01360
PGA1_c01370: PGA1_c01370 - transcription antitermination protein NusG, at 129,738 to 130,271
_c01370
Position (kb)
126
127
128
129 Strain fitness (log2 ratio)
-2
-1
0
1 at 125.808 kb on + strand, within PGA1_c01330 at 125.816 kb on - strand, within PGA1_c01330 at 125.821 kb on - strand, within PGA1_c01330 at 125.968 kb on - strand, within PGA1_c01330 at 125.968 kb on - strand, within PGA1_c01330 at 125.968 kb on - strand, within PGA1_c01330 at 126.039 kb on - strand, within PGA1_c01330 at 126.375 kb on + strand at 126.375 kb on + strand at 126.375 kb on + strand at 126.383 kb on - strand at 126.383 kb on - strand at 126.383 kb on - strand at 126.383 kb on - strand at 126.451 kb on + strand at 126.632 kb on + strand at 126.766 kb on + strand at 126.818 kb on - strand at 126.833 kb on - strand at 126.835 kb on + strand at 126.838 kb on - strand at 126.843 kb on - strand at 126.843 kb on - strand at 126.843 kb on - strand at 126.915 kb on + strand at 126.958 kb on + strand at 126.975 kb on + strand at 126.975 kb on + strand at 126.975 kb on + strand at 126.990 kb on + strand at 126.998 kb on - strand at 127.005 kb on + strand at 127.005 kb on + strand at 127.063 kb on - strand, within PGA1_c01350 at 127.071 kb on + strand, within PGA1_c01350 at 127.071 kb on + strand, within PGA1_c01350 at 127.076 kb on + strand, within PGA1_c01350 at 127.076 kb on + strand, within PGA1_c01350 at 127.079 kb on - strand, within PGA1_c01350 at 127.079 kb on - strand, within PGA1_c01350 at 127.084 kb on - strand, within PGA1_c01350 at 127.084 kb on - strand, within PGA1_c01350 at 127.084 kb on - strand, within PGA1_c01350 at 127.192 kb on + strand, within PGA1_c01350 at 127.264 kb on - strand, within PGA1_c01350 at 127.280 kb on + strand, within PGA1_c01350 at 127.281 kb on + strand, within PGA1_c01350 at 127.281 kb on + strand, within PGA1_c01350 at 127.289 kb on - strand, within PGA1_c01350 at 127.289 kb on - strand, within PGA1_c01350 at 127.289 kb on - strand, within PGA1_c01350 at 127.289 kb on - strand, within PGA1_c01350 at 127.315 kb on - strand, within PGA1_c01350 at 127.326 kb on + strand, within PGA1_c01350 at 127.440 kb on + strand, within PGA1_c01350 at 127.440 kb on + strand, within PGA1_c01350 at 127.446 kb on - strand, within PGA1_c01350 at 127.446 kb on - strand, within PGA1_c01350 at 127.522 kb on - strand, within PGA1_c01350 at 127.565 kb on - strand, within PGA1_c01350 at 127.571 kb on + strand, within PGA1_c01350 at 127.613 kb on + strand, within PGA1_c01350 at 127.613 kb on + strand, within PGA1_c01350 at 127.678 kb on - strand, within PGA1_c01350 at 127.742 kb on + strand, within PGA1_c01350 at 127.755 kb on - strand, within PGA1_c01350 at 127.781 kb on - strand, within PGA1_c01350 at 127.823 kb on + strand, within PGA1_c01350 at 127.823 kb on + strand, within PGA1_c01350 at 127.823 kb on + strand, within PGA1_c01350 at 127.823 kb on + strand, within PGA1_c01350 at 127.825 kb on + strand, within PGA1_c01350 at 127.829 kb on - strand, within PGA1_c01350 at 127.831 kb on - strand, within PGA1_c01350 at 127.833 kb on - strand, within PGA1_c01350 at 127.840 kb on + strand, within PGA1_c01350 at 127.843 kb on - strand, within PGA1_c01350 at 127.870 kb on + strand, within PGA1_c01350 at 127.878 kb on - strand, within PGA1_c01350 at 127.894 kb on - strand, within PGA1_c01350 at 127.894 kb on - strand, within PGA1_c01350 at 127.921 kb on + strand, within PGA1_c01350 at 127.921 kb on + strand, within PGA1_c01350 at 127.929 kb on - strand, within PGA1_c01350 at 127.940 kb on - strand, within PGA1_c01350 at 128.039 kb on - strand, within PGA1_c01350 at 128.039 kb on - strand, within PGA1_c01350 at 128.082 kb on + strand, within PGA1_c01350 at 128.088 kb on + strand, within PGA1_c01350 at 128.122 kb on + strand, within PGA1_c01350 at 128.122 kb on + strand, within PGA1_c01350 at 128.122 kb on + strand, within PGA1_c01350 at 128.130 kb on - strand, within PGA1_c01350 at 128.194 kb on + strand, within PGA1_c01350 at 128.242 kb on + strand, within PGA1_c01350 at 128.268 kb on - strand, within PGA1_c01350 at 128.345 kb on + strand, within PGA1_c01350 at 128.402 kb on + strand, within PGA1_c01350 at 128.403 kb on + strand, within PGA1_c01350 at 128.410 kb on - strand, within PGA1_c01350 at 128.413 kb on + strand, within PGA1_c01350 at 128.413 kb on + strand, within PGA1_c01350 at 128.413 kb on + strand, within PGA1_c01350 at 128.453 kb on + strand, within PGA1_c01350 at 128.453 kb on + strand, within PGA1_c01350 at 128.453 kb on + strand, within PGA1_c01350 at 128.453 kb on + strand, within PGA1_c01350 at 128.453 kb on + strand, within PGA1_c01350 at 128.526 kb on + strand, within PGA1_c01350 at 128.667 kb on + strand, within PGA1_c01350 at 128.668 kb on - strand, within PGA1_c01350 at 128.671 kb on + strand, within PGA1_c01350 at 128.727 kb on + strand, within PGA1_c01350 at 128.735 kb on - strand, within PGA1_c01350 at 128.761 kb on + strand, within PGA1_c01350 at 128.769 kb on - strand, within PGA1_c01350 at 128.771 kb on + strand, within PGA1_c01350 at 128.787 kb on + strand at 128.787 kb on + strand at 128.826 kb on - strand at 128.904 kb on + strand at 128.904 kb on + strand at 128.934 kb on - strand at 128.938 kb on + strand at 128.938 kb on + strand at 128.956 kb on - strand at 128.983 kb on + strand at 128.991 kb on - strand at 129.039 kb on + strand at 129.053 kb on + strand at 129.056 kb on - strand at 129.056 kb on - strand at 129.066 kb on - strand at 129.066 kb on - strand at 129.107 kb on + strand at 129.110 kb on - strand at 129.164 kb on - strand at 129.209 kb on - strand at 129.501 kb on - strand at 129.537 kb on + strand at 129.537 kb on + strand at 129.537 kb on + strand at 129.545 kb on - strand at 129.545 kb on - strand at 129.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 125,808 + PGA1_c01330 0.18 -0.1 125,816 - PGA1_c01330 0.19 -0.5 125,821 - PGA1_c01330 0.20 -0.7 125,968 - PGA1_c01330 0.40 -1.3 125,968 - PGA1_c01330 0.40 -0.4 125,968 - PGA1_c01330 0.40 +0.1 126,039 - PGA1_c01330 0.49 -2.3 126,375 + -0.8 126,375 + -0.6 126,375 + -0.0 126,383 - -0.6 126,383 - -1.2 126,383 - +0.9 126,383 - -0.7 126,451 + -0.5 126,632 + -0.1 126,766 + +1.1 126,818 - -0.2 126,833 - -0.5 126,835 + +0.0 126,838 - +0.1 126,843 - +0.5 126,843 - +0.8 126,843 - +1.1 126,915 + -1.9 126,958 + -1.3 126,975 + -0.3 126,975 + +1.6 126,975 + -0.3 126,990 + -0.1 126,998 - +0.2 127,005 + +0.0 127,005 + -0.2 127,063 - PGA1_c01350 0.12 -0.3 127,071 + PGA1_c01350 0.12 -1.6 127,071 + PGA1_c01350 0.12 +0.7 127,076 + PGA1_c01350 0.12 +0.1 127,076 + PGA1_c01350 0.12 +0.3 127,079 - PGA1_c01350 0.13 -0.1 127,079 - PGA1_c01350 0.13 +0.0 127,084 - PGA1_c01350 0.13 -2.1 127,084 - PGA1_c01350 0.13 -0.3 127,084 - PGA1_c01350 0.13 -0.1 127,192 + PGA1_c01350 0.18 -0.3 127,264 - PGA1_c01350 0.21 -0.7 127,280 + PGA1_c01350 0.22 -0.4 127,281 + PGA1_c01350 0.22 +0.3 127,281 + PGA1_c01350 0.22 -0.1 127,289 - PGA1_c01350 0.22 -1.9 127,289 - PGA1_c01350 0.22 -0.1 127,289 - PGA1_c01350 0.22 +0.0 127,289 - PGA1_c01350 0.22 -0.8 127,315 - PGA1_c01350 0.23 -0.5 127,326 + PGA1_c01350 0.24 -0.6 127,440 + PGA1_c01350 0.29 -0.6 127,440 + PGA1_c01350 0.29 -1.4 127,446 - PGA1_c01350 0.29 -0.4 127,446 - PGA1_c01350 0.29 +0.1 127,522 - PGA1_c01350 0.33 +0.2 127,565 - PGA1_c01350 0.35 +0.1 127,571 + PGA1_c01350 0.35 -1.1 127,613 + PGA1_c01350 0.37 -0.1 127,613 + PGA1_c01350 0.37 -0.5 127,678 - PGA1_c01350 0.40 -0.0 127,742 + PGA1_c01350 0.43 -0.3 127,755 - PGA1_c01350 0.43 +0.2 127,781 - PGA1_c01350 0.45 -0.1 127,823 + PGA1_c01350 0.47 -0.4 127,823 + PGA1_c01350 0.47 -0.4 127,823 + PGA1_c01350 0.47 +0.7 127,823 + PGA1_c01350 0.47 -0.5 127,825 + PGA1_c01350 0.47 -1.1 127,829 - PGA1_c01350 0.47 +1.2 127,831 - PGA1_c01350 0.47 +0.3 127,833 - PGA1_c01350 0.47 +0.3 127,840 + PGA1_c01350 0.47 -2.2 127,843 - PGA1_c01350 0.47 -0.3 127,870 + PGA1_c01350 0.49 -2.4 127,878 - PGA1_c01350 0.49 -0.5 127,894 - PGA1_c01350 0.50 -0.9 127,894 - PGA1_c01350 0.50 -0.2 127,921 + PGA1_c01350 0.51 -0.7 127,921 + PGA1_c01350 0.51 +0.9 127,929 - PGA1_c01350 0.51 -0.0 127,940 - PGA1_c01350 0.52 +0.2 128,039 - PGA1_c01350 0.56 +0.5 128,039 - PGA1_c01350 0.56 -1.1 128,082 + PGA1_c01350 0.58 -2.0 128,088 + PGA1_c01350 0.59 -1.1 128,122 + PGA1_c01350 0.60 -0.1 128,122 + PGA1_c01350 0.60 -0.3 128,122 + PGA1_c01350 0.60 -0.8 128,130 - PGA1_c01350 0.61 -0.4 128,194 + PGA1_c01350 0.63 -0.3 128,242 + PGA1_c01350 0.66 +0.1 128,268 - PGA1_c01350 0.67 +1.1 128,345 + PGA1_c01350 0.70 -1.0 128,402 + PGA1_c01350 0.73 -0.5 128,403 + PGA1_c01350 0.73 +0.5 128,410 - PGA1_c01350 0.73 -0.7 128,413 + PGA1_c01350 0.73 +0.1 128,413 + PGA1_c01350 0.73 -0.6 128,413 + PGA1_c01350 0.73 -0.7 128,453 + PGA1_c01350 0.75 +0.5 128,453 + PGA1_c01350 0.75 -0.2 128,453 + PGA1_c01350 0.75 +0.5 128,453 + PGA1_c01350 0.75 +1.0 128,453 + PGA1_c01350 0.75 -0.4 128,526 + PGA1_c01350 0.79 -1.2 128,667 + PGA1_c01350 0.85 -0.1 128,668 - PGA1_c01350 0.85 +1.1 128,671 + PGA1_c01350 0.85 -1.7 128,727 + PGA1_c01350 0.88 -0.2 128,735 - PGA1_c01350 0.88 +0.8 128,761 + PGA1_c01350 0.89 -0.7 128,769 - PGA1_c01350 0.90 -0.2 128,771 + PGA1_c01350 0.90 -0.6 128,787 + -0.9 128,787 + +1.1 128,826 - -1.2 128,904 + +1.0 128,904 + +0.6 128,934 - -0.8 128,938 + +1.3 128,938 + +0.1 128,956 - +0.3 128,983 + +0.7 128,991 - -0.2 129,039 + +0.4 129,053 + -0.3 129,056 - -0.7 129,056 - -0.1 129,066 - -0.6 129,066 - -2.2 129,107 + -0.5 129,110 - -1.3 129,164 - -1.1 129,209 - -0.8 129,501 - -1.0 129,537 + +0.7 129,537 + -0.3 129,537 + +1.5 129,545 - -0.5 129,545 - +0.2 129,545 - -0.7
Or see this region's nucleotide sequence