Experiment: Succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0410 and Rru_A0411 are separated by 172 nucleotides Rru_A0411 and Rru_A0412 are separated by 39 nucleotides Rru_A0412 and Rru_A0413 are separated by 74 nucleotides Rru_A0413 and Rru_A0414 overlap by 4 nucleotides
Rru_A0410: Rru_A0410 - DnaB helicase (NCBI), at 487,197 to 488,720
_A0410
Rru_A0411: Rru_A0411 - Ribosomal protein L9 (NCBI), at 488,893 to 489,480
_A0411
Rru_A0412: Rru_A0412 - hypothetical protein (NCBI), at 489,520 to 490,446
_A0412
Rru_A0413: Rru_A0413 - Ribosomal protein S18 (NCBI), at 490,521 to 490,757
_A0413
Rru_A0414: Rru_A0414 - Ribosomal protein S6 (NCBI), at 490,754 to 491,236
_A0414
Position (kb) 489
490
491 Strain fitness (log2 ratio) -2
-1
0
1 at 488.731 kb on + strand at 488.732 kb on - strand at 488.907 kb on - strand at 488.907 kb on - strand at 488.907 kb on - strand at 489.210 kb on - strand, within Rru_A0411 at 489.210 kb on - strand, within Rru_A0411 at 489.739 kb on - strand, within Rru_A0412 at 489.739 kb on - strand, within Rru_A0412 at 489.739 kb on - strand, within Rru_A0412 at 489.739 kb on - strand, within Rru_A0412 at 489.939 kb on - strand, within Rru_A0412 at 489.939 kb on - strand, within Rru_A0412 at 490.224 kb on - strand, within Rru_A0412 at 490.377 kb on - strand at 491.353 kb on - strand at 491.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinate (C) remove 488,731 + -1.1 488,732 - +0.2 488,907 - +0.2 488,907 - +1.2 488,907 - +1.2 489,210 - Rru_A0411 0.54 -1.8 489,210 - Rru_A0411 0.54 -0.9 489,739 - Rru_A0412 0.24 -1.4 489,739 - Rru_A0412 0.24 +1.2 489,739 - Rru_A0412 0.24 -1.1 489,739 - Rru_A0412 0.24 -0.1 489,939 - Rru_A0412 0.45 -0.0 489,939 - Rru_A0412 0.45 -0.6 490,224 - Rru_A0412 0.76 -0.4 490,377 - +1.0 491,353 - +0.1 491,353 - -0.2
Or see this region's nucleotide sequence