Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13135

Experiment: LB, pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotidesBWI76_RS13130 and BWI76_RS13135 overlap by 1 nucleotidesBWI76_RS13135 and BWI76_RS13140 are separated by 28 nucleotides BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349 _RS13125 BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761 _RS13130 BWI76_RS13135: BWI76_RS13135 - 3-oxoadipyl-CoA thiolase, at 2,738,761 to 2,739,966 _RS13135 BWI76_RS13140: BWI76_RS13140 - phenylacetate-CoA ligase, at 2,739,995 to 2,741,311 _RS13140 Position (kb) 2738 2739 2740Strain fitness (log2 ratio) -1 0 1at 2737.786 kb on + strand, within BWI76_RS13125at 2737.794 kb on - strand, within BWI76_RS13125at 2737.877 kb on + strand, within BWI76_RS13125at 2738.546 kb on + strand, within BWI76_RS13130at 2738.845 kb on - strandat 2738.935 kb on - strand, within BWI76_RS13135at 2739.311 kb on - strandat 2739.452 kb on + strand, within BWI76_RS13135at 2739.460 kb on - strand, within BWI76_RS13135at 2739.928 kb on - strandat 2739.931 kb on + strandat 2739.931 kb on + strandat 2740.433 kb on + strand, within BWI76_RS13140at 2740.799 kb on + strand, within BWI76_RS13140at 2740.865 kb on + strand, within BWI76_RS13140

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH9
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2,737,786 + BWI76_RS13125 0.60 -0.0
2,737,794 - BWI76_RS13125 0.61 -0.2
2,737,877 + BWI76_RS13125 0.67 +1.7
2,738,546 + BWI76_RS13130 0.49 -1.1
2,738,845 - +0.6
2,738,935 - BWI76_RS13135 0.14 -0.2
2,739,311 - -1.1
2,739,452 + BWI76_RS13135 0.57 -0.7
2,739,460 - BWI76_RS13135 0.58 +0.2
2,739,928 - -0.6
2,739,931 + -0.7
2,739,931 + -0.6
2,740,433 + BWI76_RS13140 0.33 +0.1
2,740,799 + BWI76_RS13140 0.61 +0.2
2,740,865 + BWI76_RS13140 0.66 +0.4

Or see this region's nucleotide sequence