Experiment: MRS + carbenicillin Time5hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12255 and BBR_RS12260 are separated by 461 nucleotides BBR_RS12260 and BBR_RS12265 are separated by 65 nucleotides
BBR_RS12255: BBR_RS12255 - hypothetical protein, at 500,083 to 500,997
_RS12255
BBR_RS12260: BBR_RS12260 - LemA family protein, at 501,459 to 502,046
_RS12260
BBR_RS12265: BBR_RS12265 - DUF2207 domain-containing protein, at 502,112 to 504,367
_RS12265
Position (kb) 501
502
503 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 500.467 kb on + strand, within BBR_RS12255 at 500.472 kb on + strand, within BBR_RS12255 at 500.475 kb on - strand, within BBR_RS12255 at 500.475 kb on - strand, within BBR_RS12255 at 500.483 kb on + strand, within BBR_RS12255 at 500.487 kb on + strand, within BBR_RS12255 at 500.487 kb on + strand, within BBR_RS12255 at 500.488 kb on + strand, within BBR_RS12255 at 500.489 kb on + strand, within BBR_RS12255 at 500.491 kb on - strand, within BBR_RS12255 at 500.496 kb on - strand, within BBR_RS12255 at 500.496 kb on - strand, within BBR_RS12255 at 500.510 kb on - strand, within BBR_RS12255 at 500.512 kb on + strand, within BBR_RS12255 at 500.524 kb on - strand, within BBR_RS12255 at 500.563 kb on - strand, within BBR_RS12255 at 500.568 kb on - strand, within BBR_RS12255 at 500.568 kb on - strand, within BBR_RS12255 at 500.568 kb on - strand, within BBR_RS12255 at 500.631 kb on + strand, within BBR_RS12255 at 500.668 kb on + strand, within BBR_RS12255 at 500.668 kb on + strand, within BBR_RS12255 at 500.683 kb on + strand, within BBR_RS12255 at 500.683 kb on + strand, within BBR_RS12255 at 500.722 kb on + strand, within BBR_RS12255 at 500.730 kb on - strand, within BBR_RS12255 at 500.783 kb on - strand, within BBR_RS12255 at 500.811 kb on + strand, within BBR_RS12255 at 500.819 kb on - strand, within BBR_RS12255 at 500.819 kb on - strand, within BBR_RS12255 at 500.819 kb on - strand, within BBR_RS12255 at 500.819 kb on - strand, within BBR_RS12255 at 500.819 kb on - strand, within BBR_RS12255 at 500.981 kb on + strand at 500.986 kb on - strand at 500.995 kb on + strand at 501.006 kb on + strand at 501.006 kb on + strand at 501.016 kb on + strand at 501.020 kb on + strand at 501.024 kb on - strand at 501.024 kb on - strand at 501.024 kb on - strand at 501.030 kb on + strand at 501.046 kb on + strand at 501.202 kb on + strand at 501.210 kb on + strand at 501.210 kb on - strand at 501.212 kb on + strand at 501.217 kb on + strand at 501.225 kb on - strand at 501.226 kb on + strand at 501.226 kb on + strand at 501.226 kb on + strand at 501.244 kb on - strand at 501.244 kb on - strand at 501.244 kb on - strand at 501.244 kb on - strand at 501.260 kb on + strand at 501.336 kb on + strand at 501.360 kb on + strand at 501.362 kb on + strand at 501.362 kb on + strand at 501.362 kb on + strand at 501.364 kb on - strand at 501.370 kb on + strand at 501.370 kb on - strand at 501.375 kb on + strand at 501.375 kb on + strand at 501.383 kb on - strand at 501.383 kb on - strand at 501.414 kb on + strand at 501.451 kb on + strand at 501.463 kb on + strand at 501.476 kb on - strand at 501.492 kb on + strand at 501.540 kb on + strand, within BBR_RS12260 at 501.548 kb on - strand, within BBR_RS12260 at 501.548 kb on - strand, within BBR_RS12260 at 501.561 kb on + strand, within BBR_RS12260 at 501.561 kb on + strand, within BBR_RS12260 at 501.561 kb on + strand, within BBR_RS12260 at 501.569 kb on - strand, within BBR_RS12260 at 501.605 kb on + strand, within BBR_RS12260 at 501.622 kb on + strand, within BBR_RS12260 at 501.638 kb on + strand, within BBR_RS12260 at 501.644 kb on - strand, within BBR_RS12260 at 501.648 kb on + strand, within BBR_RS12260 at 501.656 kb on - strand, within BBR_RS12260 at 501.656 kb on - strand, within BBR_RS12260 at 501.661 kb on - strand, within BBR_RS12260 at 501.665 kb on + strand, within BBR_RS12260 at 501.670 kb on + strand, within BBR_RS12260 at 501.670 kb on + strand, within BBR_RS12260 at 501.678 kb on - strand, within BBR_RS12260 at 501.678 kb on - strand, within BBR_RS12260 at 501.697 kb on - strand, within BBR_RS12260 at 501.729 kb on + strand, within BBR_RS12260 at 501.730 kb on + strand, within BBR_RS12260 at 501.745 kb on - strand, within BBR_RS12260 at 501.747 kb on - strand, within BBR_RS12260 at 501.776 kb on + strand, within BBR_RS12260 at 501.778 kb on + strand, within BBR_RS12260 at 501.793 kb on + strand, within BBR_RS12260 at 501.795 kb on + strand, within BBR_RS12260 at 501.796 kb on - strand, within BBR_RS12260 at 501.796 kb on - strand, within BBR_RS12260 at 501.824 kb on + strand, within BBR_RS12260 at 501.825 kb on - strand, within BBR_RS12260 at 501.834 kb on + strand, within BBR_RS12260 at 501.910 kb on - strand, within BBR_RS12260 at 501.910 kb on - strand, within BBR_RS12260 at 501.926 kb on + strand, within BBR_RS12260 at 501.946 kb on - strand, within BBR_RS12260 at 501.990 kb on + strand at 501.995 kb on + strand at 501.998 kb on - strand at 501.998 kb on - strand at 502.029 kb on + strand at 502.029 kb on + strand at 502.029 kb on + strand at 502.037 kb on - strand at 502.067 kb on + strand at 502.070 kb on + strand at 502.082 kb on + strand at 502.100 kb on + strand at 502.100 kb on + strand at 502.108 kb on - strand at 502.122 kb on + strand at 502.136 kb on + strand at 502.136 kb on + strand at 502.136 kb on + strand at 502.144 kb on - strand at 502.161 kb on - strand at 502.213 kb on - strand at 502.260 kb on + strand at 502.280 kb on + strand at 502.322 kb on - strand at 502.338 kb on + strand, within BBR_RS12265 at 502.338 kb on + strand, within BBR_RS12265 at 502.397 kb on - strand, within BBR_RS12265 at 502.404 kb on - strand, within BBR_RS12265 at 502.404 kb on - strand, within BBR_RS12265 at 502.431 kb on + strand, within BBR_RS12265 at 502.431 kb on + strand, within BBR_RS12265 at 502.434 kb on + strand, within BBR_RS12265 at 502.438 kb on - strand, within BBR_RS12265 at 502.444 kb on - strand, within BBR_RS12265 at 502.457 kb on - strand, within BBR_RS12265 at 502.459 kb on + strand, within BBR_RS12265 at 502.459 kb on + strand, within BBR_RS12265 at 502.464 kb on + strand, within BBR_RS12265 at 502.467 kb on - strand, within BBR_RS12265 at 502.467 kb on - strand, within BBR_RS12265 at 502.467 kb on - strand, within BBR_RS12265 at 502.467 kb on - strand, within BBR_RS12265 at 502.467 kb on - strand, within BBR_RS12265 at 502.481 kb on - strand, within BBR_RS12265 at 502.504 kb on - strand, within BBR_RS12265 at 502.529 kb on + strand, within BBR_RS12265 at 502.547 kb on + strand, within BBR_RS12265 at 502.547 kb on + strand, within BBR_RS12265 at 502.552 kb on + strand, within BBR_RS12265 at 502.555 kb on - strand, within BBR_RS12265 at 502.563 kb on - strand, within BBR_RS12265 at 502.573 kb on - strand, within BBR_RS12265 at 502.575 kb on + strand, within BBR_RS12265 at 502.575 kb on + strand, within BBR_RS12265 at 502.575 kb on + strand, within BBR_RS12265 at 502.580 kb on - strand, within BBR_RS12265 at 502.583 kb on - strand, within BBR_RS12265 at 502.583 kb on - strand, within BBR_RS12265 at 502.583 kb on - strand, within BBR_RS12265 at 502.583 kb on - strand, within BBR_RS12265 at 502.583 kb on - strand, within BBR_RS12265 at 502.585 kb on - strand, within BBR_RS12265 at 502.591 kb on - strand, within BBR_RS12265 at 502.622 kb on + strand, within BBR_RS12265 at 502.622 kb on + strand, within BBR_RS12265 at 502.630 kb on - strand, within BBR_RS12265 at 502.644 kb on - strand, within BBR_RS12265 at 502.672 kb on + strand, within BBR_RS12265 at 502.680 kb on - strand, within BBR_RS12265 at 502.680 kb on - strand, within BBR_RS12265 at 502.687 kb on + strand, within BBR_RS12265 at 502.704 kb on + strand, within BBR_RS12265 at 502.705 kb on - strand, within BBR_RS12265 at 502.733 kb on - strand, within BBR_RS12265 at 502.769 kb on + strand, within BBR_RS12265 at 502.771 kb on + strand, within BBR_RS12265 at 502.776 kb on + strand, within BBR_RS12265 at 502.777 kb on - strand, within BBR_RS12265 at 502.781 kb on + strand, within BBR_RS12265 at 502.781 kb on + strand, within BBR_RS12265 at 502.781 kb on + strand, within BBR_RS12265 at 502.787 kb on - strand, within BBR_RS12265 at 502.796 kb on - strand, within BBR_RS12265 at 502.798 kb on - strand, within BBR_RS12265 at 502.804 kb on - strand, within BBR_RS12265 at 502.820 kb on - strand, within BBR_RS12265 at 502.823 kb on + strand, within BBR_RS12265 at 502.824 kb on + strand, within BBR_RS12265 at 502.879 kb on - strand, within BBR_RS12265 at 502.886 kb on + strand, within BBR_RS12265 at 502.886 kb on + strand, within BBR_RS12265 at 502.894 kb on - strand, within BBR_RS12265 at 502.919 kb on - strand, within BBR_RS12265 at 502.919 kb on - strand, within BBR_RS12265 at 502.946 kb on + strand, within BBR_RS12265 at 502.954 kb on - strand, within BBR_RS12265 at 502.954 kb on - strand, within BBR_RS12265 at 502.954 kb on - strand, within BBR_RS12265 at 502.957 kb on + strand, within BBR_RS12265 at 502.959 kb on + strand, within BBR_RS12265 at 502.960 kb on - strand, within BBR_RS12265 at 502.960 kb on - strand, within BBR_RS12265 at 502.977 kb on - strand, within BBR_RS12265
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + carbenicillin Time5hr remove 500,467 + BBR_RS12255 0.42 -1.9 500,472 + BBR_RS12255 0.43 +0.4 500,475 - BBR_RS12255 0.43 -1.0 500,475 - BBR_RS12255 0.43 +0.5 500,483 + BBR_RS12255 0.44 +1.2 500,487 + BBR_RS12255 0.44 -1.4 500,487 + BBR_RS12255 0.44 -2.6 500,488 + BBR_RS12255 0.44 -1.3 500,489 + BBR_RS12255 0.44 +0.1 500,491 - BBR_RS12255 0.45 -1.2 500,496 - BBR_RS12255 0.45 +0.6 500,496 - BBR_RS12255 0.45 +0.6 500,510 - BBR_RS12255 0.47 -2.1 500,512 + BBR_RS12255 0.47 -0.4 500,524 - BBR_RS12255 0.48 +1.6 500,563 - BBR_RS12255 0.52 -3.6 500,568 - BBR_RS12255 0.53 +0.4 500,568 - BBR_RS12255 0.53 +0.5 500,568 - BBR_RS12255 0.53 +0.5 500,631 + BBR_RS12255 0.60 -0.4 500,668 + BBR_RS12255 0.64 +0.4 500,668 + BBR_RS12255 0.64 -0.8 500,683 + BBR_RS12255 0.66 +0.9 500,683 + BBR_RS12255 0.66 +2.4 500,722 + BBR_RS12255 0.70 -2.1 500,730 - BBR_RS12255 0.71 -0.2 500,783 - BBR_RS12255 0.77 +1.9 500,811 + BBR_RS12255 0.80 +0.8 500,819 - BBR_RS12255 0.80 -0.7 500,819 - BBR_RS12255 0.80 -0.5 500,819 - BBR_RS12255 0.80 -0.5 500,819 - BBR_RS12255 0.80 +2.2 500,819 - BBR_RS12255 0.80 -0.0 500,981 + -1.1 500,986 - -0.6 500,995 + +0.1 501,006 + +1.0 501,006 + -1.0 501,016 + +1.7 501,020 + +1.4 501,024 - -1.0 501,024 - +2.2 501,024 - -0.6 501,030 + -1.1 501,046 + +0.4 501,202 + +0.4 501,210 + -2.5 501,210 - -0.6 501,212 + +1.4 501,217 + +0.7 501,225 - +2.9 501,226 + -0.6 501,226 + -0.6 501,226 + -0.1 501,244 - -0.7 501,244 - +1.0 501,244 - +1.3 501,244 - -0.2 501,260 + +0.1 501,336 + +0.3 501,360 + +2.0 501,362 + +1.9 501,362 + -0.7 501,362 + -0.6 501,364 - -2.2 501,370 + +0.6 501,370 - +0.1 501,375 + -0.1 501,375 + -0.6 501,383 - +0.6 501,383 - +0.6 501,414 + +1.4 501,451 + +0.4 501,463 + -2.2 501,476 - -1.2 501,492 + -0.6 501,540 + BBR_RS12260 0.14 +1.0 501,548 - BBR_RS12260 0.15 -0.4 501,548 - BBR_RS12260 0.15 +0.4 501,561 + BBR_RS12260 0.17 +0.4 501,561 + BBR_RS12260 0.17 -0.3 501,561 + BBR_RS12260 0.17 -0.7 501,569 - BBR_RS12260 0.19 +0.1 501,605 + BBR_RS12260 0.25 -0.0 501,622 + BBR_RS12260 0.28 -0.6 501,638 + BBR_RS12260 0.30 +0.4 501,644 - BBR_RS12260 0.31 -2.2 501,648 + BBR_RS12260 0.32 -1.4 501,656 - BBR_RS12260 0.34 -2.5 501,656 - BBR_RS12260 0.34 -1.2 501,661 - BBR_RS12260 0.34 +0.8 501,665 + BBR_RS12260 0.35 +1.0 501,670 + BBR_RS12260 0.36 -1.2 501,670 + BBR_RS12260 0.36 +3.6 501,678 - BBR_RS12260 0.37 -2.2 501,678 - BBR_RS12260 0.37 -0.6 501,697 - BBR_RS12260 0.40 -1.6 501,729 + BBR_RS12260 0.46 -2.6 501,730 + BBR_RS12260 0.46 -0.1 501,745 - BBR_RS12260 0.49 -0.7 501,747 - BBR_RS12260 0.49 -0.3 501,776 + BBR_RS12260 0.54 -0.0 501,778 + BBR_RS12260 0.54 -1.6 501,793 + BBR_RS12260 0.57 -0.6 501,795 + BBR_RS12260 0.57 +1.7 501,796 - BBR_RS12260 0.57 -1.1 501,796 - BBR_RS12260 0.57 -1.1 501,824 + BBR_RS12260 0.62 +0.6 501,825 - BBR_RS12260 0.62 +0.4 501,834 + BBR_RS12260 0.64 +0.3 501,910 - BBR_RS12260 0.77 +1.9 501,910 - BBR_RS12260 0.77 +0.0 501,926 + BBR_RS12260 0.79 -0.9 501,946 - BBR_RS12260 0.83 -0.1 501,990 + +3.5 501,995 + +1.8 501,998 - +0.6 501,998 - +0.4 502,029 + -0.7 502,029 + +0.7 502,029 + +1.7 502,037 - -0.6 502,067 + +0.5 502,070 + +1.8 502,082 + -3.2 502,100 + -0.5 502,100 + -1.5 502,108 - +0.9 502,122 + -0.3 502,136 + +1.0 502,136 + +0.4 502,136 + -2.5 502,144 - -0.3 502,161 - -0.2 502,213 - +1.0 502,260 + -1.6 502,280 + -1.3 502,322 - +1.1 502,338 + BBR_RS12265 0.10 -0.2 502,338 + BBR_RS12265 0.10 +0.6 502,397 - BBR_RS12265 0.13 -1.4 502,404 - BBR_RS12265 0.13 +1.3 502,404 - BBR_RS12265 0.13 -1.5 502,431 + BBR_RS12265 0.14 +0.6 502,431 + BBR_RS12265 0.14 -0.0 502,434 + BBR_RS12265 0.14 +2.4 502,438 - BBR_RS12265 0.14 -1.3 502,444 - BBR_RS12265 0.15 -0.4 502,457 - BBR_RS12265 0.15 +2.2 502,459 + BBR_RS12265 0.15 +0.4 502,459 + BBR_RS12265 0.15 -4.3 502,464 + BBR_RS12265 0.16 -0.6 502,467 - BBR_RS12265 0.16 -0.0 502,467 - BBR_RS12265 0.16 -2.6 502,467 - BBR_RS12265 0.16 +0.9 502,467 - BBR_RS12265 0.16 -0.4 502,467 - BBR_RS12265 0.16 +1.0 502,481 - BBR_RS12265 0.16 +1.0 502,504 - BBR_RS12265 0.17 +1.4 502,529 + BBR_RS12265 0.18 -0.1 502,547 + BBR_RS12265 0.19 -1.2 502,547 + BBR_RS12265 0.19 +3.7 502,552 + BBR_RS12265 0.20 +1.2 502,555 - BBR_RS12265 0.20 +1.4 502,563 - BBR_RS12265 0.20 +2.5 502,573 - BBR_RS12265 0.20 +0.4 502,575 + BBR_RS12265 0.21 -0.3 502,575 + BBR_RS12265 0.21 +0.4 502,575 + BBR_RS12265 0.21 -0.2 502,580 - BBR_RS12265 0.21 +0.1 502,583 - BBR_RS12265 0.21 -0.3 502,583 - BBR_RS12265 0.21 -0.6 502,583 - BBR_RS12265 0.21 +0.2 502,583 - BBR_RS12265 0.21 +0.4 502,583 - BBR_RS12265 0.21 -1.1 502,585 - BBR_RS12265 0.21 -0.6 502,591 - BBR_RS12265 0.21 +1.4 502,622 + BBR_RS12265 0.23 +1.5 502,622 + BBR_RS12265 0.23 +0.3 502,630 - BBR_RS12265 0.23 -0.5 502,644 - BBR_RS12265 0.24 +0.2 502,672 + BBR_RS12265 0.25 +1.2 502,680 - BBR_RS12265 0.25 -1.6 502,680 - BBR_RS12265 0.25 +2.2 502,687 + BBR_RS12265 0.25 -2.1 502,704 + BBR_RS12265 0.26 +0.8 502,705 - BBR_RS12265 0.26 +1.7 502,733 - BBR_RS12265 0.28 +1.6 502,769 + BBR_RS12265 0.29 -2.3 502,771 + BBR_RS12265 0.29 -1.2 502,776 + BBR_RS12265 0.29 +0.2 502,777 - BBR_RS12265 0.29 -0.0 502,781 + BBR_RS12265 0.30 -2.8 502,781 + BBR_RS12265 0.30 -0.6 502,781 + BBR_RS12265 0.30 +2.7 502,787 - BBR_RS12265 0.30 +0.4 502,796 - BBR_RS12265 0.30 -0.0 502,798 - BBR_RS12265 0.30 +0.3 502,804 - BBR_RS12265 0.31 +2.2 502,820 - BBR_RS12265 0.31 -1.3 502,823 + BBR_RS12265 0.32 -1.5 502,824 + BBR_RS12265 0.32 +2.1 502,879 - BBR_RS12265 0.34 +1.0 502,886 + BBR_RS12265 0.34 -1.4 502,886 + BBR_RS12265 0.34 -0.6 502,894 - BBR_RS12265 0.35 -0.4 502,919 - BBR_RS12265 0.36 -1.2 502,919 - BBR_RS12265 0.36 -1.6 502,946 + BBR_RS12265 0.37 +1.0 502,954 - BBR_RS12265 0.37 +2.2 502,954 - BBR_RS12265 0.37 +1.0 502,954 - BBR_RS12265 0.37 +0.4 502,957 + BBR_RS12265 0.37 +0.4 502,959 + BBR_RS12265 0.38 +0.4 502,960 - BBR_RS12265 0.38 +0.1 502,960 - BBR_RS12265 0.38 +1.4 502,977 - BBR_RS12265 0.38 -4.1
Or see this region's nucleotide sequence